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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 管永恕(Yuan-Shu Kuan) | |
| dc.contributor.author | Bang-Chi Lin | en |
| dc.contributor.author | 林邦齊 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:37:53Z | - |
| dc.date.available | 2015-08-11 | |
| dc.date.copyright | 2015-08-11 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-04-14 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54755 | - |
| dc.description.abstract | Wnt ligand是一個在多細胞生物中被廣泛使用之蛋白質訊息分子。Wnt所誘發的訊息傳遞路徑被廣泛使用在眾多生物現象之中，包含胚胎早期發育、幹細胞多型性維持和細胞癌化。對於Wnt訊息所引發之反應已有廣泛的研究，但關於Wnt如何被製造和如何自細胞釋放等的詳細資訊仍有所不足。Wntless蛋白被報導負責將Wnt由高基氏體運送至細胞膜的攜帶蛋白質，Wntless存在與否對於多數Wnt之分泌皆有重大影響。透過研究Wntless，我們即可從側面探索Wnt如何在細胞中被輸送和釋放。儘管數個Wntless的功能性區段已被報導，但Wntless羧基端區段在斑馬魚中所扮演之功能仍然尚未釐清。在本實驗室先前研究中推測Wntless的羧基端150個胺基酸殘基對於其功能扮演重要角色。而在本研究中，我進一步針對Wntless最末端49個胺基酸區塊設計切除版本，並且將受部分切除的mRNAs以顯微注射的方式使其表現在Wntless突變的斑馬魚胚胎中。實驗組中，表現末端缺乏的Wntless和單獨只有最末端兩種版本皆無法拯救wls突變之斑馬魚胚胎，據此我推測Wntless羧基端為其生物功能必須之區段。此外，我使用特定辨識羧基端之抗體來進行免疫螢光染色，發現單獨只剩羧基端的切除版本和切除中間第221至420胺基酸區段的Wntless之細胞內分布與全長的Wntless不同。據此我推測這兩個切除版本的切除區段皆為Wntless在細胞之中被正確運送所必須。 | zh_TW |
| dc.description.abstract | Wnt is a secreted ligand and involved in numerous cell-cell signaling processes, especially in embryo development and tissue homeostasis. Comparing to the understanding of the responding signaling in Wnt-receiving cells, the sorting and secretion of Wnt in Wnt-producing cell is less clear. Wntless (Wls, also known as Gpr177 in mammal) plays a crucial role in carrying Wnts from Golgi to plasma membrane. Although several important functional motifs of Wls have been identified, whether the carboxyl-terminal domain of Wls is functional in zebrafish embryonic development is unknown. I have evaluated the abilities of different truncated Wls proteins in rescuing the inner ear and jaw cartilage defects of wls mutants. I found that neither the carboxyl-terminal 49 amino acids deleted form nor the form containing only the carboxyl-terminal 49 a.a rescue the defects of wls mutants. These data indicate that the carboxyl terminal 49 amino acids are important for Wls function in modulating Wls-mediated development in zebrafish embryo. In addition, utilizing antibody against Wls carboxyl-terminal 49 amino acids, I found the subcellular location of the truncated Wls containing only the carboxyl-terminal 49 a.a or carrying a.a. 221-420 deletion is distinct from endogenous Wls. I suggest these deleted region are both required for Wls subcellular sorting. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:37:53Z (GMT). No. of bitstreams: 1 ntu-104-R01b46023-1.pdf: 2160890 bytes, checksum: 05d036ff3d9bef1d0dc10d5ee6f0a64c (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 中文摘要 vii
Abstract viii Chapter 1 Introduction 1 1.1 Wnt and Wnt signal pathway 1 1.2 Intracellular trafficking of Wnt and Wntless 2 1.3 Regulation of Wls 4 1.4 The C-terminal domain of Wls is important for its biological function in rescuing jaw cartilage and ear development 5 Chapter 2 Material and Methods 7 2.1 Primers design and PCR 7 2.2 Wls mutant fish 8 2.3 Jaw cartilage alcian blue staining 8 2.4 Quantification of Phenotypes 9 2.4.1 Jaw cartilage and fish standard length 9 2.4.2 Inner ear area 9 2.5 Capped mRNA in vitro transcription 10 2.6 Microinjection of fish egg 10 2.7 Yeast two-hybrid 11 2.8 Whole mount Immunohistochemistry of fish embryos 11 Chapter 3 Result 13 3.1 Inner ear size and jaw cartilage is chosen as a mutant phenotype indicator 13 3.2 Carboxyl-terminal domain deleted Wls failed to rescue loss-of-Wls phenotype. 14 3.3 Expression of truncated Wls is confirmed by anti-Wls antibody immunostaining 15 3.4 Interaction partner of Wls C-terminal domain is still waiting for further research 17 Chapter 4 Discussion 18 4.1 The carboxyl-terminal 49 amino acids are required for Wls function 18 4.2 The subcellular location of Wls 20 4.3 The reasons of failure in finding interaction protein in yeast two-hybrid screening 22 References 24 Table & Figures 29 | |
| dc.language.iso | en | |
| dc.subject | Wnt signaling | zh_TW |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | Wntless | zh_TW |
| dc.subject | Wnt | zh_TW |
| dc.subject | Wntless | en |
| dc.subject | Zebrafish | en |
| dc.subject | Retromer | en |
| dc.subject | Wnt | en |
| dc.subject | Wnt signaling pathway | en |
| dc.title | Wntless蛋白之羧基端功能在斑馬魚胚胎發育扮演角色之探討 | zh_TW |
| dc.title | Investigating the in vivo role of Carboxyl-terminal Domain of Wntless protein in Zebrafish embryonic development | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 胡清華(Chin-Hwa Hu),黃銓珍(Chang-Jen Huang),黃聲蘋(Sheng-ping Hwang) | |
| dc.subject.keyword | 斑馬魚,Wntless,Wnt,Wnt signaling, | zh_TW |
| dc.subject.keyword | Wnt,Wnt signaling pathway,Wntless,Retromer,Zebrafish, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-04-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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