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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭典翰 | |
| dc.contributor.author | Wei-Yu Tao | en |
| dc.contributor.author | 陶威宇 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:36:57Z | - |
| dc.date.available | 2025-05-26 | |
| dc.date.copyright | 2015-08-11 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-05-27 | |
| dc.identifier.citation | Dale, L., Howes, G., Price, B. M., & Smith, J. C. (1992). Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development. Development, 115(2), 573-585.
Ferguson, E. L., & Anderson, K. V. (1992). Decapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo. Cell, 71(3), 451-461. Finnerty, J. R., Pang, K., Burton, P., Paulson, D., & Martindale, M. Q. (2004). Origins of bilateral symmetry: Hox and dpp expression in a sea anemone. Science, 304(5675), 1335-1337. doi: 10.1126/science.1091946 Francois, V., Solloway, M., O'Neill, J. W., Emery, J., & Bier, E. (1994). Dorsal-ventral patterning of the Drosophila embryo depends on a putative negative growth factor encoded by the short gastrulation gene. Genes Dev, 8(21), 2602-2616. Gawlik, K., Shiryaev, S. A., Zhu, W., Motamedchaboki, K., Desjardins, R., Day, R., . . . Strongin, A. Y. (2009). Autocatalytic activation of the furin zymogen requires removal of the emerging enzyme's N-terminus from the active site. PLoS One, 4(4), e5031. doi: 10.1371/journal.pone.0005031 Gurdon, J. B., & Bourillot, P. Y. (2001). Morphogen gradient interpretation. Nature, 413(6858), 797-803. doi: 10.1038/35101500 Huang, F. Z., & Weisblat, D. A. (1996). Cell fate determination in an annelid equivalence group. Development, 122(6), 1839-1847. Hwang, J. R., Siekhaus, D. E., Fuller, R. S., Taghert, P. H., & Lindberg, I. (2000). Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2. Insect cell-specific processing and secretion. J Biol Chem, 275(23), 17886-17893. doi: 10.1074/jbc.M000032200 Kuo, D. H., & Weisblat, D. A. (2011a). A new molecular logic for BMP-mediated dorsoventral patterning in the leech Helobdella. Current Biology, 21(15), 1282-1288. doi: 10.1016/j.cub.2011.06.024 Kuo, D. H., & Weisblat, D. A. (2011b). A New Molecular Logic for BMP-Mediated Dorsoventral Patterning in the Leech Helobdella. Current Biology, 21(15), 1282-1288. doi: DOI 10.1016/j.cub.2011.06.024 Matus, D. Q., Pang, K., Marlow, H., Dunn, C. W., Thomsen, G. H., & Martindale, M. Q. (2006). Molecular evidence for deep evolutionary roots of bilaterality in animal development. Proc Natl Acad Sci U S A, 103(30), 11195-11200. doi: 10.1073/pnas.0601257103 Mesnard, D., & Constam, D. B. (2010). Imaging proprotein convertase activities and their regulation in the implanting mouse blastocyst. Journal of Cell Biology, 191(1), 129-139. doi: DOI 10.1083/jcb.201005026 Mizutani, C. M., & Bier, E. (2008). EvoD/Vo: the origins of BMP signalling in the neuroectoderm. Nat Rev Genet, 9(9), 663-677. doi: 10.1038/nrg2417 Navarre, J., Bradford, A. J., Calhoun, B. C., & Goldenring, J. R. (1996). Quenching of endogenous peroxidase in western blot. Biotechniques, 21(6), 990-992. Sasai, Y., Lu, B., Steinbeisser, H., Geissert, D., Gont, L. K., & De Robertis, E. M. (1994). Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Cell, 79(5), 779-790. Seidah, N. G. (2011). The proprotein convertases, 20 years later. Methods Mol Biol, 768, 23-57. doi: 10.1007/978-1-61779-204-5_3 Seidah, N. G., & Prat, A. (2012). The biology and therapeutic targeting of the proprotein convertases. Nat Rev Drug Discov, 11(5), 367-383. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nat Methods, 2(12), 905-909. doi: 10.1038/nmeth819 Srinivasan, S., Rashka, K. E., & Bier, E. (2002). Creation of a Sog morphogen gradient in the Drosophila embryo. Dev Cell, 2(1), 91-101. Thomas, G. (2002). Furin at the cutting edge: From protein traffic to embryogenesis and disease. Nature Reviews Molecular Cell Biology, 3(10), 753-766. doi: Doi 10.1038/Nrm934 Weisblat, D. A., & Kuo, D. H. (2009). Helobdella (leech): a model for developmental studies. Cold Spring Harb Protoc, 2009(4), pdb emo121. doi: 10.1101/pdb.emo121 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54702 | - |
| dc.description.abstract | BMP訊息路徑在動物胚胎體軸發育上扮演重要角色，目前已知其訊息傳遞的範圍，受前驅蛋白酶對BMP前驅蛋白的切割方式所影響。在脊椎動物和果蠅上，BMP4�Dpp均以擴散形成濃度梯度的形式調控胚胎背腹體軸建立，但在澤蛭系統中，BMP5-8才是調控神經外胚層背腹體軸的關鍵BMP訊息分子，其作用方式也不同於前兩者，必須透過細胞間的直接接觸產生作用。先前研究得知，澤蛭不同細胞系譜中的BMP訊息傳遞範圍也有差異。我們可以分析BMP5-8前驅蛋白之切割處理來進一步瞭解水蛭胚胎體軸建立的上游分子機制。本研究分析澤蛭中十二個前驅蛋白轉換酶（proprotein convertase, PCs）與已知的前驅蛋白轉換酶家族之親緣關係，並以反轉錄PCR和RNA原位雜交染色法，確立澤蛭中十二個前驅蛋白轉換酶的表現。為測試前驅蛋白轉換酶的活性，嘗試以細胞表面聯結蛋白水解標示法（cell surface-linked indicator of proteolysis assay, CLIP），以及異位表現切位定點突變的受標定BMP5-8兩種方法分析，結果發現切位突變後的BMP，並不影響BMP在建立神經外胚層背腹體軸的功能。 | zh_TW |
| dc.description.abstract | Bone morphogenetic protein (BMP) morphogen is known to play a vital role in body axis patterning during embryogenesis. Unlike BMP4/Dpp functions as a morphogen patterning embryo dorsoventral (DV) axis in vertebrate and fly, BMP5-8 in leech, the key BMP signaling ligand specifies DV patterning, acts in a “cell-cell contact” fashion. Evidences indicate that the ligand BMP proprotein’s differential proteolytic processing by proprotein convertases (PCs or PCSKs) influences its signaling range. Further, different signaling ranges were found between teloblast lineages (N/Q and O/P), above which make the proteolytic processing of BMP5-8 in leech embryo DV patterning an unsolved question. To characterize the role of PCSK-mediated cleavage in leech DV patterning, I carried out the following experiments. First, by reverse transcription-PCR and whole mount in situ hybridization, expressions of all 12 PCSK-related genes in leech have been confirmed and localized at embryogenesis stage 8. Second, a cell surface-linked indicator of proteolysis assay (CLIP) is prepared to test PC activity in vivo. Third, ectopic expression of cleavage site-mutated BMP5-8 in leech has been used. The above results indicate that the proteolytic processing of BMP5-8 is not required for development of normal neuroectodermal, which is also a key event of body axis formation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:36:57Z (GMT). No. of bitstreams: 1 ntu-104-R00b41026-1.pdf: 8514563 bytes, checksum: 25d9b79d17ae4275f0f6a96c6280327f (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 口試委員會審定書．．．I
誌謝．．．II 中文摘要．．．III ABSTRACT．．．IV CONTENT．．．V INTRODUCTION．．．1 MATERIALS AND METHODS．．．6 Leech husbandry．．．6 Identification and isolation of PCSK genes．．．6 RT-PCR analysis．．．8 Cleavage-resistant mutation of BMP．．．9 CLIP expression．．．12 Western blotting．．．15 Histological analyses．．．16 RESULTS．．．18 Identification of PCSK genes in Helobdella genome．．．18 Phylogenetic analysis of metazoan PCSK genes．．．19 Developmental expression of Helobdella PCSK genes．．．21 Furin-mediated cleavage is not required for BMP signaling activity in germinal band．．．23 Monitoring BMP cleavage in germinal band tissue．．．24 DISCUSSION．．．28 REFERENCES．．．33 FIGURES and TABLES．．．37 | |
| dc.language.iso | en | |
| dc.subject | 澤蛭 | zh_TW |
| dc.subject | PCSK蛋白 | zh_TW |
| dc.subject | 背腹體軸發育 | zh_TW |
| dc.subject | 骨形態發生蛋白 | zh_TW |
| dc.subject | BMP | en |
| dc.subject | Helobdella | en |
| dc.subject | Dorsoventral patterning | en |
| dc.subject | Proprotein convertase subtilisin/kexin- like | en |
| dc.subject | Leech | en |
| dc.title | 澤蛭神經外胚層背腹體軸發育過程中PCSK表現與BMP前驅蛋白調控之初探 | zh_TW |
| dc.title | Expression of PCSK and Proteolytic Processing of BMP Propeptide on Dorso-Ventral Patterning of Helobdella Neuroectoderm: a Preliminary Study | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳俊宏,游智凱 | |
| dc.subject.keyword | 澤蛭,骨形態發生蛋白,背腹體軸發育,PCSK蛋白, | zh_TW |
| dc.subject.keyword | Leech,Helobdella,BMP,Dorsoventral patterning,Proprotein convertase subtilisin/kexin- like, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-05-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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