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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳志毅(Jyh-Yih Chen) | |
dc.contributor.author | Yi-Da Wang | en |
dc.contributor.author | 王亦大 | zh_TW |
dc.date.accessioned | 2021-06-16T02:57:51Z | - |
dc.date.available | 2020-07-20 | |
dc.date.copyright | 2015-07-20 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-07-07 | |
dc.identifier.citation | 1. Zapata A, Diez B, Cejalvo T, Gutierrez-de Frias C, Cortes A: Ontogeny of the immune system of fish. Fish Shellfish Immunol. 2006, 20(2):126-136.
2. Schr?der MB, Villena AJ, J?rgensen TØ: Ontogeny of lymphoid organs and immunoglobulin producing cells in Atlantic cod (Gadus morhua L.). Dev Comp Immunol. 1998, 22(5):507-517. 3. Lovy J, Savidant GP, Wright GM: Ontogeny and disease responses of Langerhans-like cells in lymphoid tissues of salmonid fish. Cell Tissue Res. 2011, 346(1):111-118. 4. Wei S, Huang Y, Cai J, Huang X, Fu J, Qin Q: Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides. Fish Shellfish Immunol. 2012, 33(2):186-196. 5. Lee K-K: Pathogenesis studies on Vibrio alginolyticus in the grouper, Epinephelus malabaricus, Bloch et Schneider. Microb Pathog. 1995, 19(1):39-48. 6. Sunyer JO, Boshra H, Lorenzo G, Parra D, Freedman B, Bosch N: Evolution of complement as an effector system in innate and adaptive immunity. Immunol Res. 2003, 27(2-3):549-564. 7. Sunyer JO, Zarkadis I, Sarrias MR, Hansen JD, Lambris JD: Cloning, structure, and function of two rainbow trout Bf molecules. J Immunol. 1998, 161(8):4106-4114. 8. Li J, Barreda DR, Zhang Y-A, Boshra H, Gelman AE, LaPatra S, Tort L, Sunyer JO: B lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities. Nat Immunol. 2006, 7(10):1116-1124. 9. Rieger AM, Hall BE, Barreda DR: Macrophage activation differentially modulates particle binding, phagocytosis and downstream antimicrobial mechanisms. Dev Comp Immunol. 2010, 34(11):1144-1159. 10. Delamarre L, Pack M, Chang H, Mellman I, Trombetta ES: Differential lysosomal proteolysis in antigen-presenting cells determines antigen fate. Science 2005, 307(5715):1630-1634. 11. Briggs RT, Drath DB, Karnovsky ML, Karnovsky MJ: Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J Cell Biol. 1975, 67(3):566-586. 12. Boltana S, Donate C, Goetz FW, MacKenzie S, Balasch JC: Characterization and expression of NADPH oxidase in LPS-, poly (I: C)-and zymosan-stimulated trout (Oncorhynchus mykiss W.) macrophages. Fish Shellfish Immunol. 2009, 26(4):651-661. 13. Rieger AM, Barreda DR: Antimicrobial mechanisms of fish leukocytes. Dev Comp Immunol. 2011, 35(12):1238-1245. 14. Roca FJ, Mulero I, López-Muñoz A, Sepulcre MP, Renshaw SA, Meseguer J, Mulero V: Evolution of the inflammatory response in vertebrates: Fish TNF-α is a powerful activator of endothelial cells but hardly activates phagocytes. J Immunol. 2008, 181(7):5071-5081. 15. Olavarría VH, Gallardo L, Figueroa JE, Mulero V: Lipopolysaccharide primes the respiratory burst of Atlantic salmon SHK-1 cells through protein kinase C-mediated phosphorylation of p47phox. Dev Comp Immunol. 2010, 34(12):1242-1253. 16. Neumann NF, Barreda DR, Belosevic M: Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures. Fish Shellfish Immunol. 2000, 10(1):1-20. 17. Kaplan JE, Chrenek RD, Morash JG, Ruksznis CM, Hannum LG: Rhythmic patterns in phagocytosis and the production of reactive oxygen species by zebrafish leukocytes. Comp Biochem Physiol A Mol Integr Physiol. 2008, 151(4):726-730. 18. MacMicking J, Xie Q-w, Nathan C: Nitric oxide and macrophage function. Annu Rev Immunol. 1997, 15(1):323-350. 19. Barroso JB, Carreras A, Esteban FJ, Peinado MA, Martínez-Lara E, Valderrama R, Jiménez A, Rodrigo J, Lupiáñez JA: Molecular and kinetic characterization and cell type location of inducible nitric oxide synthase in fish. Am J Physiol Regul Integr Comp Physiol. 2000, 279(2):R650-R656. 20. Saeij JP, Stet RJ, Groeneveld A, Verburg-van Kemenade LB, van Muiswinkel WB, Wiegertjes GF: Molecular and functional characterization of a fish inducible-type nitric oxide synthase. Immunogenetics 2000, 51(4-5):339-346. 21. Hanington PC, Hitchen SJ, Beamish LA, Belosevic M: Macrophage colony stimulating factor (CSF-1) is a central growth factor of goldfish macrophages. Fish Shellfish Immunol. 2009, 26(1):1-9. 22. Forlenza M, Magez S, Scharsack JP, Westphal A, Savelkoul HF, Wiegertjes GF: Receptor-mediated and lectin-like activities of carp (Cyprinus carpio) TNF-α. J Immunol. 2009, 183(8):5319-5332. 23. Stafford J, Neumann N, Belosevic M: Products of proteolytic cleavage of transferrin induce nitric oxide response of goldfish macrophages. Dev Comp Immunol. 2001, 25(2):101-115. 24. Haddad G, Hanington PC, Wilson EC, Grayfer L, Belosevic M: Molecular and functional characterization of goldfish (Carassius auratus L.) transforming growth factor beta. Dev Comp Immunol. 2008, 32(6):654-663. 25. Pietsch C, Vogt R, Neumann N, Kloas W: Production of nitric oxide by carp (Cyprinus carpio L.) kidney leukocytes is regulated by cyclic 3′, 5′-adenosine monophosphate. Comp Biochem Physiol A Mol Integr Physiol. 2008, 150(1):58-65. 26. Saeij JP, van Muiswinkel WB, van de Meent M, Amaral C, Wiegertjes GF: Different capacities of carp leukocytes to encounter nitric oxide-mediated stress: a role for the intracellular reduced glutathione pool. Dev Comp Immunol. 2003, 27(6):555-568. 27. Trachootham D, Lu W, Ogasawara MA, Valle NR-D, Huang P: Redox regulation of cell survival. Antioxid Redox Sign. 2008, 10(8):1343-1374. 28. Mayumi M, Takeda Y, Hoshiko M, Serada K, Murata M, Moritomo T, Takizawa F, Kobayashi I, Araki K, Nakanishi T: Characterization of teleost phagocyte NADPH oxidase: molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase. Mol Immunol. 2008, 45(6):1720-1731. 29. Forlenza M, Scharsack JP, Kachamakova NM, Taverne-Thiele AJ, Rombout JH, Wiegertjes GF: Differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in a host–parasite animal model. Mol Immunol. 2008, 45(11):3178-3189. 30. Faurschou M, Borregaard N: Neutrophil granules and secretory vesicles in inflammation. Microbes Infect. 2003, 5(14):1317-1327. 31. Wernersson S, Reimer JM, Poorafshar M, Karlson U, Wermenstam N, Bengtén E, Wilson M, Pilström L, Hellman L: Granzyme-like sequences in bony fish shed light on the emergence of hematopoietic serine proteases during vertebrate evolution. Dev Comp Immunol. 2006, 30(10):901-918. 32. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A: Neutrophil extracellular traps kill bacteria. science 2004, 303(5663):1532-1535. 33. Palić D, Ostojić J, Andreasen CB, Roth JA: Fish cast NETs: neutrophil extracellular traps are released from fish neutrophils. Dev Comp Immunol. 2007, 31(8):805-816. 34. Casadei E, Wang T, Zou J, Vecino JLG, Wadsworth S, Secombes CJ: Characterization of three novel β-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss). Mol Immunol. 2009, 46(16):3358-3366. 35. Cuesta A, Meseguer J, Esteban MÁ: The antimicrobial peptide hepcidin exerts an important role in the innate immunity against bacteria in the bony fish gilthead seabream. Mol Immunol. 2008, 45(8):2333-2342. 36. Mulero I, Noga EJ, Meseguer J, García-Ayala A, Mulero V: The antimicrobial peptides piscidins are stored in the granules of professional phagocytic granulocytes of fish and are delivered to the bacteria-containing phagosome upon phagocytosis. Dev Comp Immunol. 2008, 32(12):1531-1538. 37. Pan C-Y, Chen J-Y, Cheng Y-SE, Chen C-Y, Ni I-H, Sheen J-F, Pan Y-L, Kuo C-M: Gene expression and localization of the epinecidin-1 antimicrobial peptide in the grouper (Epinephelus coioides), and its role in protecting fish against pathogenic infection. DNA Cell Biol. 2007, 26(6):403-413. 38. Huang P-H, Chen J-Y, Kuo C-M: Three different hepcidins from tilapia, Oreochromis mossambicus: analysis of their expressions and biological functions. Mol Immunol. 2007, 44(8):1922-1934. 39. Tort L, Balasch J, Mackenzie S: Fish immune system. A crossroads between innate and adaptive responses. Inmunología 2003, 22(3):277-286. 40. Fletcher T, Grant P: Immunoglobulins in the serum and mucus of the plaice (Pleuronectes platessa). Biochem J. 1969, 115(5):65P. 41. Danilova N, Bussmann J, Jekosch K, Steiner LA: The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z. Nat Immunol. 2005, 6(3):295-302. 42. Mardis ER: The impact of next-generation sequencing technology on genetics. Trends Genet. 2008, 24(3):133-141. 43. Di Pinto A, Ciccarese G, Tantillo G, Catalano D, Forte VT: A collagenase-targeted multiplex PCR assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus. J Food Prot. 2005, 68(1):150-153. 44. Sakakura Y, Shiotani S, Chuda H, Hagiwara A: Improvement of the survival in the seven‐band grouper Epinephelus septemfasciatus larvae by optimizing aeration and water inlet in the mass‐scale rearing tank. Fisheries sci. 2006, 72(5):939-947. 45. Mu Y, Ding F, Cui P, Ao J, Hu S, Chen X: Transcriptome and expression profiling analysis revealed changes of multiple signaling pathways involved in immunity in the large yellow croaker during Aeromonas hydrophila infection. BMC genomics 2010, 11(1):506. 46. Zhu Y, Thangamani S, Ho B, Ding JL: The ancient origin of the complement system. EMBO J. 2005, 24(2):382-394. 47. Al-Sharif WZ, Sunyer JO, Lambris JD, Smith LC: Sea urchin coelomocytes specifically express a homologue of the complement component C3. J Immunol. 1998, 160(6):2983-2997. 48. Nonaka M: Evolution of the Complement System. In: MACPF/CDC Proteins-Agents of Defence, Attack and Invasion. Springer; 2014: 31-43. 49. Boshra H, Gelman AE, Sunyer JO: Structural and functional characterization of complement C4 and C1s-like molecules in teleost fish: insights into the evolution of classical and alternative pathways. J Immunol. 2004, 173(1):349-359. 50. Boshra H, Li J, Sunyer J: Recent advances on the complement system of teleost fish. Fish Shellfish Immunol. 2006, 20(2):239-262. 51. Sunyer JO, Zarkadis IK, Sahu A, Lambris JD: Multiple forms of complement C3 in trout that differ in binding to complement activators. Proc Natl Acad Sci U S A. 1996, 93(16):8546-8551. 52. Sunyer J, Tort L, Lambris JD: Structural C3 diversity in fish: characterization of five forms of C3 in the diploid fish Sparus aurata. J Immunol. 1997, 158(6):2813-2821. 53. Nakao M, Mutsuro J, Obo R, Fujiki K, Nonaka M, Yano T: Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio): presence of variants lacking the catalytic histidine. Eur J Immunol. 2000, 30(3):858-866. 54. Holland MCH, Lambris JD: The complement system in teleosts. Fish Shellfish Immunol. 2002, 12(5):399-420. 55. Ellis A: Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp Immunol. 2001, 25(8):827-839. 56. Sunyer JO, Tort L: Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are effected by the alternative complement pathway. Vet Immunol Immunopathol. 1995, 45(3):333-345. 57. Wang T, Secombes CJ: Complete sequencing and expression of three complement components, C1r, C4 and C1 inhibitor, of the classical activation pathway of the complement system in rainbow trout Oncorhynchus mykiss. Immunogenetics 2003, 55(9):615-628. 58. Sunyer JO, Zarkadis IK, Lambris JD: Complement diversity: a mechanism for generating immune diversity? Immunol Today. 1998, 19(11):519-523. 59. Qi Z-H, Liu Y-F, Wang W-N, Wu X, Xin Y, Lu Y-F, Wang A-L: Molecular characterization and functional analysis of a complement C3 molecule in the orange-spotted grouper (Epinephelus coioides). Fish Shellfish Immunol. 2011, 31(6):1284-1290. 60. Müller-Eberhard HJ: The killer molecule of complement. Br J Dermatol. 1985, 85:47s-52s. 61. Franchini S, Zarkadis IK, Sfyroera G, Sahu A, Moore WT, Mastellos D, LaPatra SE, Lambris JD: Cloning and purification of the rainbow trout fifth component of complement (C5). Dev Comp Immunol. 2001, 25(5):419-430. 62. Sunyer J, TORT L, Lambris J: Diversity of the third form of complement, C3, in fish: functional characterization of five forms of C3 in the diploid fish Sparus aurata. Biochem J 1997, 326:877-881. 63. Nonaka M, Yamaguchi N, Natsuume-Sakai S, Takahashi M: The complement system of rainbow trout (Salmo gairdneri). I. Identification of the serum lytic system homologous to mammalian complement. J Immunol. 1981, 126(4):1489-1494. 64. Nakao M, Uemura T, Yano T: Terminal components of carp complement constituting a membrane attack complex. Mol Immunol. 1996, 33(11):933-937. 65. Kemper C, Zipfel PF, Gigli I: The complement cofactor protein (SBP1) from the barred sand bass (Paralabrax nebulifer) mediates overlapping regulatory activities of both human C4b binding protein and factor H. J Biol Chem. 1998, 273(31):19398-19404. 66. Nakao M, Hisamatsu S, Nakahara M, Kato Y, Smith SL, Yano T: Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio). Immunogenetics 2003, 54(11):801-806. 67. Neumann NF, Stafford JL, Barreda D, Ainsworth AJ, Belosevic M: Antimicrobial mechanisms of fish phagocytes and their role in host defense. Dev Comp Immunol. 2001, 25(8):807-825. 68. GANASSIN R, BOLS N: Development of long-term rainbow trout spleen cultures that are haemopoietic and produce dendritic cells. Fish Shellfish Immunol. 1996, 6(1):17-34. 69. Vasta GR, Nita-Lazar M, Giomarelli B, Ahmed H, Du S, Cammarata M, Parrinello N, Bianchet MA, Amzel LM: Structural and functional diversity of the lectin repertoire in teleost fish: relevance to innate and adaptive immunity. Dev Comp Immunol. 2011, 35(12):1388-1399. 70. Rodrıguez A, Esteban M, Meseguer J: A mannose-receptor is possibly involved in the phagocytosis of Saccharomyces cerevisiae by seabream (Sparus aurata L.) leucocytes. Fish Shellfish Immunol. 2003, 14(5):375-388. 71. Herbomel P, Thisse B, Thisse C: Ontogeny and behaviour of early macrophages in the zebrafish embryo. Development 1999, 126(17):3735-3745. 72. Romano N, Picchietti S, Taverne-Thiele J, Taverne N, Abelli L, Mastrolia L, Verburg-van Kemenade B, Rombout J: Distribution of macrophages during fish development: an immunohistochemical study in carp (Cyprinus carpio, L.). Anat Embryol (Berl). 1998, 198(1):31-41. 73. Brogden KA: Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol. 2005, 3(3):238-250. 74. Douglas SE, Gallant JW, Liebscher RS, Dacanay A, Tsoi SC: Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish. Dev Comp Immunol. 2003, 27(6):589-601. 75. Qu H, Chen B, Peng H, Wang K: Molecular cloning, recombinant expression, and antimicrobial activity of EC-hepcidin3, a new four-cysteine hepcidin isoform from Epinephelus coioides. Biosci Biotechnol Biochem. 2013, 77(1):103-110. 76. Nemeth E, Valore EV, Territo M, Schiller G, Lichtenstein A, Ganz T: Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein. Blood 2003, 101(7):2461-2463. 77. Hsieh J-C, Pan C-Y, Chen J-Y: Tilapia hepcidin (TH) 2-3 as a transgene in transgenic fish enhances resistance to Vibrio vulnificus infection and causes variations in immune-related genes after infection by different bacterial species. Fish Shellfish Immunol. 2010, 29(3):430-439. 78. Sepulcre MP, Alcaraz-Pérez F, López-Muñoz A, Roca FJ, Meseguer J, Cayuela ML, Mulero V: Evolution of lipopolysaccharide (LPS) recognition and signaling: fish TLR4 does not recognize LPS and negatively regulates NF-κB activation. J Immunol. 2009, 182(4):1836-1845. 79. Li Y-W, Luo X-C, Dan X-M, Huang X-Z, Qiao W, Zhong Z-P, Li A-X: Orange-spotted grouper (Epinephelus coioides) TLR2, MyD88 and IL-1β involved in anti-Cryptocaryon irritans response. Fish Shellfish Immunol. 2011, 30(6):1230-1240. 80. Basu M, Swain B, Maiti N, Routray P, Samanta M: Inductive expression of toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp, mrigal (Cirrhinus mrigala). Fish Shellfish Immunol. 2012, 32(1):121-131. 81. Laing KJ, Secombes CJ: Chemokines. Dev Comp Immunol. 2004, 28(5):443-460. 82. Bruna-Romero O, Schmieg J, Del Val M, Buschle M, Tsuji M: The dendritic cell-specific chemokine, dendritic cell-derived CC chemokine 1, enhances protective cell-mediated immunity to murine malaria. J Immunol. 2003, 170(6):3195-3203. 83. Su Y, Guo Z, Xu L, Jiang J, Wang J, Feng J: Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response. Fish Shellfish Immunol. 2012, 32(1):204-210. 84. Lin C-Y, Chen Y-M, Hsu H-H, Shiu C-T, Kuo H-C, Chen T-Y: Grouper (Epinephelus coioides) CXCR4 is expressed in response to pathogens infection and early stage of development. Dev Comp Immunol. 2012, 36(1):112-120. 85. Hsu Y-J, Hou C-Y, Lin S-J, Kuo W-C, Lin H-T, Lin JH-Y: The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity. Fish Shellfish Immunol. 2013, 35(6):1891-1898. 86. Alejo A, Tafalla C: Chemokines in teleost fish species. Dev Comp Immunol. 2011, 35(12):1215-1222. 87. Laing KJ, Secombes CJ: Trout CC chemokines: comparison of their sequences and expression patterns. Mol Immunol. 2004, 41(8):793-808. 88. Schutyser E, Struyf S, Van Damme J: The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 2003, 14(5):409-426. 89. Khokha R, Murthy A, Weiss A: Metalloproteinases and their natural inhibitors in inflammation and immunity. Nat Rev Immunol. 2013, 13(9):649-665. 90. Volkman HE, Pozos TC, Zheng J, Davis JM, Rawls JF, Ramakrishnan L: Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium. Science 2010, 327(5964):466-469. 91. Uhlar CM, Whitehead AS: Serum amyloid A, the major vertebrate acute‐phase reactant. Eur J Biochem. 1999, 265(2):501-523. 92. Fujiki K, Shin D-H, Nakao M, Yano T: Molecular cloning and expression analysis of carp (Cyprinus carpio) interleukin-1β, high affinity immunoglobulin E Fc receptor γ subunit and serum amyloid A. Fish Shellfish Immunol. 2000, 10(3):229-242. 93. Lawler J: Thrombospondin‐1 as an endogenous inhibitor of angiogenesis and tumor growth. J Cell Mol Med. 2002, 6(1):1-12. 94. Vallejo AN, Mügge LO, Klimiuk PA, Weyand CM, Goronzy JJ: Central role of thrombospondin-1 in the activation and clonal expansion of inflammatory T cells. J Immunol. 2000, 164(6):2947-2954. 95. Oshima M, Dinchuk JE, Kargman SL, Oshima H, Hancock B, Kwong E, Trzaskos JM, Evans JF, Taketo MM: Suppression of intestinal polyposis in Apc Δ716 knockout mice by inhibition of cyclooxygenase 2 (COX-2). Cell 1996, 87(5):803-809. 96. Ishikawa To, Herschman HR: Two inducible, functional cyclooxygenase‐2 genes are present in the rainbow trout genome. J Cell Biochem. 2007, 102(6):1486-1492. 97. Opal SM, Esmon CT: Bench-to-bedside review: functional relationships between coagulation and the innate immune response and their respective roles in the pathogenesis of sepsis. Crit Care. 2002, 7(1):23. 98. Pietretti D, Spaink HP, Falco A, Forlenza M, Wiegertjes GF: Accessory molecules for Toll-like receptors in Teleost fish. Identification of TLR4 interactor with leucine-rich repeats (TRIL). Mol Immunol. 2013, 56(4):745-756. 99. Yi M-J, Park S-H, Cho H-N, Yong Chung H, Kim J-I, Cho C-K, Lee S-J, Lee Y-S: Heat-shock protein 25 (Hspb1) regulates manganese superoxide dismutase through activation of Nfkb (NF-κB). Radiat Res. 2002, 158(5):641-649. 100. van Noort JM, Bsibsi M, Nacken P, Gerritsen WH, Amor S: The link between small heat shock proteins and the immune system. Int J Biochem Cell Biol. 2012, 44(10):1670-1679. 101. Ojima N: Rainbow trout hspb1 (hsp27): identification of two mRNA splice variants that show predominant expression in muscle tissues. Comp Biochem Physiol B Biochem Mol Biol. 2007, 148(3):277-285. 102. Middleton RC, Shelden EA: Small heat shock protein HSPB1 regulates growth of embryonic zebrafish craniofacial muscles. Exp Cell Res. 2013, 319(6):860-874. 103. Li B, Tournier C, Davis RJ, Flavell RA: Regulation of IL‐4 expression by the transcription factor JunB during T helper cell differentiation. EMBO J. 1999, 18(2):420-432. 104. Wagner EF, Eferl R: Fos/AP‐1 proteins in bone and the immune system. Immunol Rev. 2005, 208(1):126-140. 105. Ewart KV, Williams J, Richards RC, Gallant JW, Melville K, Douglas SE: The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish. Dev Comp Immunol. 2008, 32(4):380-390. 106. Ellis A: Immunity to bacteria in fish. Fish Shellfish Immunol. 1999, 9(4):291-308. 107. Lieschke GJ, Oates AC, Crowhurst MO, Ward AC, Layton JE: Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish. Blood 2001, 98(10):3087-3096. 108. Gu Y, Parker A, Wilson TM, Bai H, Chang D-Y, Lu A-L: Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6. J Biol Chem. 2002, 277(13):11135-11142. 109. Teachey DT, Seif AE, Brown VI, Bruno M, Bunte RM, Chang YJ, Choi JK, Fish JD, Hall J, Reid GS: Targeting Notch signaling in autoimmune and lymphoproliferative disease. Blood 2008, 111(2):705-714. 110. Lorent K, Yeo S-Y, Oda T, Chandrasekharappa S, Chitnis A, Matthews RP, Pack M: Inhibition of Jagged-mediated Notch signaling disrupts zebrafish biliary development and generates multi-organ defects compatible with an Alagille syndrome phenocopy. Development 2004, 131(22):5753-5766. 111. Melby TE, Ciampaglio CN, Briscoe G, Erickson HP: The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge. J Cell Biol. 1998, 142(6):1595-1604. 112. Boggs J: Myelin basic protein: a multifunctional protein. Cell Mol Life Sci. 2006, 63(17):1945-1961. 113. Lider O, Santos L, Lee C, Higgins P, Weiner H: Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. II. Suppression of disease and in vitro immune responses is mediated by antigen-specific CD8+ T lymphocytes. J Immunol. 1989, 142(3):748-752. 114. Khoury S, Hancock W, Weiner H: Oral tolerance to myelin basic protein and natural recovery from experimental autoimmune encephalomyelitis are associated with downregulation of inflammatory cytokines and differential upregulation of transforming growth factor beta, interleukin 4, and prostaglandin E expression in the brain. J Exp Med. 1992, 176(5):1355-1364. 115. Wang Y-D, Huang S-J, Chou H-N, Liao W-L, Gong H-Y, Chen J-Y: Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity-related complement pathway in Epinephelus coioides. BMC genomics 2014, 15(1):1102. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54454 | - |
dc.description.abstract | 點帶石斑 (Epinephelus coioides) 在台灣是一種極富經濟潛力的養殖魚種。然而在幼苗時期的石斑魚對細菌病原體如溶藻弧菌很容易受到感染。為了更加了解溶藻弧菌感染點帶石斑幼苗的免疫機制,我們使用了高通量次世代定序技術來研究感染時的基因表現,總讀值為114,851,002,共有9,687,355,560核苷酸,總計209,082個重疊群,平均長度為372bp。Gene ontology (GO) 分析在 transcriptome 中顯示出12組細胞組成 (cellular component)群集,16組分子功能 (molecular function)的群集,以及42組生物過程 (biological process)的群集 (P value < 0.05)。共有32664 點帶石斑的基因在京都基因與基因组百科全書 (KEGG)被比對出來。1504不同表現的基因 (DEGs)在12個群集中被辨識出來 (P value < 0.05)。弧菌感染造成的基因表現包含補體系統、凝血因子、金黃色葡萄球菌感染、吞噬體活性、抗原呈獻系統以及抗原表現途徑。我們總結出在先天性免疫反應中的補體路徑以及抗菌胜肽 hepicidin可能在點帶石斑對抗溶藻弧菌感染之時扮演了重要的角色,其免疫反應的啟動時間約在感染後四個小時左右,這些結果顯示石斑魚在早期發育階段,溶藻弧菌感染時可能誘發補體反應,增進了我們對弧菌感染點帶石斑其免疫機制的瞭解。
為了更進一步了解其他種類的石斑魚幼苗感染溶藻弧菌的情況,我們使用了具有高經濟價值的龍膽石斑 (Epinephelus lanceolatus) 幼苗進行高通量次世代定序技術,結果顯示,檢測出的總讀值為28,705,411,共有2,152,905,850 個base,組出的unigene數目共有100,848個,經過篩選後剩餘5,913 unigene (篩選方式為 FPKM>0.3, 2FC, p<0.05)。GO分析中,細胞組成 (cellular component)共有30個GO 數量,生物過程 (biological process)以及分子功能 (molecular function)分別各有58個GO數量。從中挑選出與免疫相關的群組,生物過程中參與免疫反應(immune response)群組的 unigene 總共有27個,免疫系統過程 (immune system process)有31個, 發炎反應 (inflammatory response)有9個,壓力反應 (response to stress)有43個 unigene。細胞組成中參與膜攻擊複合物 (membrane attack complex; MAC)有8個unigene。分子功能中參與趨化因子活性 (chemokine activity)以及趨化因子受體結合(chemokine receptor binding)分別各有9個,細胞因子 (cytokine activity)有13個,細胞因子受體結合 (cytokine receptor binding) 有12個unigene。而在KEGG的分析上,共有47個pathway。由於從 KEGG的分析資料中所解出的pathway 所參與的基因數量偏低,只有1到4個unigene參與其中,無法從KEGG pathway中挑選出可能與免疫相關的途徑。因此最終以GO 所分析出的免疫基因利用GeneSpring連結出可能的pathway。從Real-time PCR的比對中得知TLR5、IL-1β、IL-8、SAA以及hepcidin 都具有大量表現,時間平均分布在4到16小時之間,整理後比對參考文獻以及GeneSpring 規畫出可能的路徑圖,其結果顯示感染途徑可能由溶藻弧菌的鞭毛刺激TLR5的活化,由MyD88 經一連串的訊息傳遞,最後刺激NFκB 產生如IL-1β、IL-8 誘導前發炎反應 (proinflammatory)或是趨化性 (chemotactic)。另一方面也可能經由serum amyloid A 刺激嗜中性球 (neutrophils) 到感染部位分泌MMP9 對IL-8進行剪切與活化,活化後的IL-8則會增強嗜中性球的化學毒性。而C3、C6、C7、C8以及C9的基因表現也顯示出補體系統同時也會受到刺激,組成MAC分解細菌細胞膜,抗菌胜肽 hepcidin的大量表現也可能殺死溶藻弧菌。結果表明其免疫調控路徑可能是經由TLR5調控下游cytokine的表現。總結以上言論,點帶石斑幼苗感染溶藻弧菌會啟動補體系統以及抗菌胜肽hepcidin的表現,而龍膽石斑幼苗感染相同細菌除上述兩種途徑外,更發現溶藻弧菌可能其鞭毛會誘導TLR5的強烈反應,調控後續細胞因子以及發炎反應的進行。 | zh_TW |
dc.description.abstract | Orange-spotted grouper (Epinephelus coioides) with protogynous hermaphroditic features are one of the most economically important aquaculture species in Taiwan. However, larvae stage grouper are susceptible to infection by the bacterial pathogen Vibrio alginolyticus. To better understand the molecular mechanisms of the immune response to V. alginolyticus in Epinephelus coioides larvae, we used high-throughput deep sequencing technology to study the effect of infection on gene expression. A total of 114,851,002 reads were assembled, consisting of 9,687,355,560 nucleotides; these were further assembled into 209,082 contigs with a mean length of 372 bp. Gene ontology (GO) analysis of the transcriptome revealed 12 cellular component subcategories, 16 molecular function subcategories, and 42 biological process subcategories (P value < 0.05). A total of 32,664 Epinephelus coioides genes were mapped to the Kyoto Encyclopedia of Genes and Genomes (KEGG); 1,504 differentially expressed genes (DEGs) were subsequently identified, in 12 categories (P value < 0.05). Vibrio infection affected the expression of genes involved in complementation, coagulation cascades, pathogen (Staphylococcus aureus) infection, phagosome activity, antigen processing, and the antigen presentation pathway. We conclude that the complement pathway of innate immunity and the hepicidin antimicrobial peptide may play important roles in the defense of Epinephelus coioides larvae against V. alginolyticus, and the immune response may activate at 4h after bacterial infection. These results implicate the complement pathway signal pathway in immunity during V. alginolyticus infection at early developmental stages, enhancing our understanding of the mechanisms underlying the immune response to Vibrio infection in Epinephelus coioides.
To realize other kind of grouper, we choose Epinephelus lanceolatus which were higher economic value then Epinephelus coioides, we have established and conducted first-round annotation of transcriptome profiles in V. alginolyticus -infected and non-infected Epinephelus lanceolatus larva. The result shown that total read value became to 28,705,411, 2,152,905,850 total base, 100,848 unigenes number, 5,913 unigenes number filter for FPKM>0.3, 2FC, p<0.05. On the GO analysis, total 30 GO numbers in cellular component, both biological process and molecular function are 58 GO numbers. We choose the cluster of immune pathway, the cluster of biological process contains 27 unigenes in immune response, 31 unigenes in immune system process, 9 unigenes in inflammatory response, and 43 unigenes in response to stress. Cellular component contain 8 unigenes in membrane attack complex. Both chemokine activity and chemokine receptors binding contain 9 unigenes in molecular function, cytokine activity obtained 13 and cytokine receptor binding contain 12 unigenes. On the KEGG analysis, contain 47 total pathways. Because of KEGG pathway involved lower then GO analysis, only include 1-4 genes. Finally we choose GO analysis became a main sign decided the pathway by GeneSpring software. The result of Real-time qPCR shown that TLR5, IL-1β, IL-8, SAA and hepcidin consisted strong expression between 4 to 16h, and indicate that V. alginolyticus probably stimulate TLR5 activity by bacterial flagellum, through by MyD88 dependent pathway, finally produce JunB, IL-1βand IL-8 from NFκB pathway induce pro-inflammatory effects or chemotactic effects. Otherwise possibility via by serum amyloid A stimulate neutrophils to infect tissue secret MMP9 then cleave IL-8 and to activate. IL-8 would enhance neutrophil chemotaxis. The express gene from C3, C6, C7, C8 and C9 indicate they would induction complement system and become a membrane attack complex to lysis bacteria membrane. Antimicrobial peptide contain highly expression possibility destroy the V. alginolyticus. The result indicate that immune pathway of V. alginolyticus infected Epinephelus lanceolatus probably via TLR5 regulate down- stream cytokine gene expression. Summarize two species of grouper, Epinephelus coioides infected by V. alginolyticus stimulated complement system and hepcidin expression, in Epinephelus lanceolatus both above-mentioned immune pathway also activation. Moreover, V. alginolyticus probably stimulated TLR5 by flagellum to mediate cytokine and inflammatory response. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T02:57:51Z (GMT). No. of bitstreams: 1 ntu-104-D99b45002-1.pdf: 6477067 bytes, checksum: 4397ae247e25e1b185ed1dedccf37613 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝…………………………………………………………………………....…….......i
中文摘要………………………………………………………………………....….......ii 英文摘要………………………………………………………………………..............iv 目錄................................................................................................................................vii 圖目錄...............................................................................................................................x 表目錄............................................................................................................................xii 第一章、序言…………………………………………………………………..1 第二章、文獻整理…………………………………………………………………....4 1. 石斑魚簡介………………………………..…..……………………….……….4 2. 溶藻弧菌介紹......................................................................................................5 3. 硬骨魚類的免疫系統表現..................................................................................5 4. 魚類抗菌機制......................................................................................................5 5. 補體系統..............................................................................................................6 6. 吞噬體融合..........................................................................................................6 7. 呼吸爆..................................................................................................................7 8. 一氧化氮的作用..................................................................................................8 9. 發炎反應..............................................................................................................9 10. 嗜中性顆粒球、巨噬細胞、單核球以及B細胞...........................................9 11. 嗜中性顆粒球與胞外陷阱 (extracellular traps).............................................10 12. 抗菌胜肽..........................................................................................................10 13. 專一性免疫......................................................................................................11 14. 數位基因表現..................................................................................................11 第三章、實驗材料.........................................................................................................12 1. 實驗動物...........................................................................................................12 2. 定量PCR檢測所篩選的基因以及專一性引子.............................................12 3. 細菌株...............................................................................................................12 4. 實驗用試劑與器材...........................................................................................12 5. 儀器設備...........................................................................................................13 6. 使用軟體...........................................................................................................13 第四章、實驗方法........................................................................................................15 1. 細菌株培養.......................................................................................................15 2. 感染試驗...........................................................................................................15 3. RNA萃取以及溶藻弧菌PCR檢測.................................................................16 4. Transcriptome......................................................................................................16 5. Unigene的GO分類..........................................................................................16 6. Unigene之細胞代謝通路分析..........................................................................17 7. RNA轉cDNA...................................................................................................17 8. Real-Time PCR分析..........................................................................................17 第五章、實驗結果.........................................................................................................19 1. 以NGS分析點帶石斑幼苗感染溶藻弧菌後之基因表現..............................19 2. 經由GO以及KEGG分析辨識不同基因的表現...........................................19 3. 點帶石斑幼苗中感染溶藻弧菌的細菌數量....................................................20 4. 分析受感染的魚免疫相關訊息傳遞路徑........................................................20 5. 分析在補體相關路徑的基因表現....................................................................20 6. 分析在吞噬作用相關路徑的基因表現............................................................21 7. 分析抗菌胜肽的基因表現................................................................................22 8. 從KEGG分析補體與吞噬作用相關路徑.......................................................23 9. 龍膽石斑幼苗感染溶藻弧菌的NGS分析......................................................23 10. 分析cytokine、chemokine、抗菌胜肽、發炎相關蛋白以及補體相關的基因表現.......................................................................................................................24 11. 分析stress response相關基因的表現...........................................................26 12. 分析龍膽石斑幼苗感染溶藻弧菌可能的免疫表現路徑............................27 第六章、實驗討論.......................................................................................................28 1. 點帶石斑幼苗感染溶藻弧菌其補體相關基因所產生的調控路徑..............28 2. 點帶石斑幼苗感染溶藻弧菌在抗菌胜肽表現上的研究..............................30 3. 龍膽石斑幼苗感染溶藻弧菌在各個免疫基因表現上的研究......................30 4. 結論..................................................................................................................35 第七章、已經發表的期刊...........................................................................................36 第八章、參考文獻.......................................................................................................37 第九章、附錄(圖).........................................................................................................52 第十章、附錄(表).........................................................................................................73 | |
dc.language.iso | zh-TW | |
dc.title | 利用Transcriptome分析技術了解點帶石斑以及龍膽石斑魚苗感染溶藻弧菌其先天免疫的調控機制 | zh_TW |
dc.title | Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity in Epinephelus coioides and Epinephelus lanceolatus | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 許祖法(Cho-Fat Hui),韓玉山(Yu-Shan Han),陳健祺(Jian-Chyi Chen),潘婕玉(Chieh-Yu Pan) | |
dc.subject.keyword | Transcriptome,溶藻弧菌,補體路徑,TLR5,qPCR, | zh_TW |
dc.subject.keyword | Transcriptome,Vibrio alginolyticus,complement pathway,TLR5,qPCR., | en |
dc.relation.page | 112 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-07-07 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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