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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 王佩華(Pei-Hwa Wang) | |
dc.contributor.author | Bo-Chun Hsu | en |
dc.contributor.author | 許博淳 | zh_TW |
dc.date.accessioned | 2021-05-15T17:58:48Z | - |
dc.date.available | 2019-03-09 | |
dc.date.available | 2021-05-15T17:58:48Z | - |
dc.date.copyright | 2014-03-09 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-02-14 | |
dc.identifier.citation | 石憲諭。2007。台灣黑豬屠體與肉質性狀之探討。碩士論文。國立屏東科技大學畜產學研究所。屏東縣。
行政院農業委員會統計室。2010。臺灣地區養豬頭數調查報告。行政院農業委員會。台北市。 行政院農業委員會統計室。2012。農業統計年報。行政院農業委員會。台北市。 宋永義、李登元、劉瑞珍、陳聯民、黃添美、徐淑芳。1988。改良臺灣小耳種豬為實驗動物。第一屆家畜遺傳育種研討會專輯。第119~134頁。台南市。 呂秀英。2010a。超級市場影響消費者購買黑豬肉之因素分析與相關性檢定。臺灣銀行季刊 61(1):130-154。 呂秀英。2010b。影響傳統市場消費者購買黑豬肉之因素分析與相關性檢定。臺灣銀行季刊 61(3) 137-159。 黃存厚。2011。高畜黑豬加工與利用。臺灣農畜產工業股份有限公司。屏東市。 許晉賓、張伸、詹嬿嫆、黃憲榮、王治華、凃海南、陳佳萱、吳明哲、張秀鑾、王政騰。2011。多產豬種之選育: I.高畜黑豬之性能。畜產研究 44(2):139-152。 張秀鑾、鄒會良、池雙慶。2001。品種與遺傳改良。畜牧要覽養豬篇增修版。第36-45頁。中國畜牧學會。華香園出版社,台北市。 陳文賢、吳祥雲、涂榮珍、紀學斌。2007。黑豬肉質特性及感官品評探討。畜產研究 40(4):241-248。 陳文賢、陳義雄、李茂盛。2001。桃園仔豬與梅山仔豬供作烘烤乳豬及成豬肉質性狀分析。畜產研究 34(1):13-20。 陳怡蓁。2007。臺灣黑豬屠體性狀與體型及遺傳變異相關之分析。碩士論文。國立臺灣大學動物科學技術學研究所。台北市。 廖宗文、蔡金生、劉建甫、蘇天明。2004。增加母豬懷孕後期飼料餵量對畜試黑豬一號繁殖性能效果評估。畜產研究 37(2):205-210。 戴謙、張秀鑾、黃鈺嘉、顏念慈。1997。臺灣本地種之性能及種原利用。畜產研究30(3):215-229。 顏念慈、李恆夫、蘇天明、蘇清全、吳淑芬、劉建甫、蔡金生、謝昭賢、廖宗文、張秀鑾、吳明哲、王政騰。2001。畜試黑豬一號繁殖。行政院農委會畜產試驗所。台南市。 顏念慈、蔡金生、蘇天明、劉建甫、李茂盛、陳添福、黃鈺嘉、陳義雄、張秀鑾、戴謙、池雙慶。2003。梅山豬經濟性能之初期觀察。畜產研究 36(3):233-244。 Andrews, D. L., B. Beames, M. D. Summers, and D. W. Park 1988. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. J. Biol. Chem. 252: 199-206. Bejerholm, C., and P. Barton-Gade. 1986. Effect of intramuscular fat level on eating quality of pig meat. Page 389-391 in Proc. 30th Europ. Meet. Meat Res. Workers, Bristol, UK.. Campbell, S. E., N. N. Tandon, G. Woldegiorgis, J. J. Luiken, J. F. Glatz, and A. Bonen. 2004. A novel function for fatty acid translocase (FAT)/CD36 involvement in long chain fatty acid transfer into the mitochondria. J. Biol. Chem. 279: 36235-36241. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159. Dobrzyn, A., and P. Dobrzyn. 2006. Stearoyl-CoA desaturase-a new player in skeletal muscle metabolism regulation. J. Physiol. Phar. 57: 31-42. Faucitano, L., J. Rivest, J. P. Daigle, J. Levesque, and C. Gariepy. 2004. Distribution of intramuscular fat content and marbling within the longissimus muscle of pigs. Can. J. Anim. Sci. 84: 57-61. Fernandez, X., G. Monin, A. Talmant, J. Mourot, and B. Lebret. 1999. Influence of intramuscular fat content on the quality of pig meat—1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum. Meat Sci. 53: 59-65. Fredrickson, D. S., R. I. Levy, and R. S. Lees. 1967. Fat transport in lipoproteins—an integrated approach to mechanisms and disorders. New England J. Med. 276: 34-44. Fulmer, W., and S. Writer. 2009. Not so FAS. SciBX. 2: (11); doi:10.1038/scibx.2009.430. Gao, S. Z., and S. M. Zhao. 2009. Physiology, affecting factors and strategies for control of pig meat intramuscular fat. Recent Pat. Food Nutr. Agric. 1: 59-74. Gerbens, F., G. Rettenberger, J. A. Lenstra, J. H. Veerkamp, and M. F. te Pas. 1997. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mamm. Genome 8: 328-332. Gerbens, F., D. J. De-Koning, F. L. Harders, T. H. Meuwissen, L. L. Janss, M. A. Groenen, and M. F. te Pas. 2000. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J. Anim. Sci. 78: 552-559. Gotthardt, M., M. Trommsdorff, M. F. Nevitt, J. Shelton, J. A. Richardson, W. Stockinger, and J. Herz. 2000. Interactions of the low density lipoprotein receptor gene family with cytosolic adaptor and scaffold proteins suggest diverse biological functions in cellular communication and signal transduction J. Biol. Chem. 275: 25616-25624. Granneman, J. G., H. H. P. Moore, L. G. Rachel, A. S. Greenberg, and Z. Zhu. 2007. Analysis of lipolytic protein trafficking and interactions. J. Biol. Chem. 282: 5726-5735. Guillet-Deniau, I., A. L. Pichard, A. Kone, C. Esnous, M. Nieruchalski, J. Girard, and C. Prip-Buus. 2004. Glucose induces de novo lipogenesis in rat muscle satellite cells through a sterol-regulatory-element-binding-protein-1c-dependent pathway. J. Cell Sci. 117: 1937-1944. Hertzel V. A., and A. D. Bernlohr. 2000. The mammalian fatty acid-binding protein multigene family: molecular and genetic insights into function. Trends Endocrin. Met. 11: 175-180. Hocquette, J. F., F. Gondret, E. Baeza, F. Medale, C. Jurie, and D. W. Pethick. 2010. Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Animal 4: 303-319. Horton, J. D., J. L. Goldstein, and S. M. Brown. 2002. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J. Clin. Invest. 109: 1125-1131. Horowitz, J. F. 2003. Fatty acid mobilization from adipose tissue during exercise. Trends Endocrinol. Metab. 14: 386-392. Huang, Q. C., X. Y. Han, Z. R. Xu, X. Y. Yang, T. Chen, and X. T. Zheng. 2009. Betaine suppresses carnitine palmitoyltransferase I in skeletal muscle but not in liver of finishing pigs. Livest. Sci. 126: 130-135. Jones, S. J., A. Guru, V. Singh, and T. F. Jones. 2005. Porcine Myology (software available from http://porcine.unl.edu). University of Nebraska, Lincoln/National Pork Board. USA. Kim, J. B., P. Sarraf, M. Wright, K. M. Yao, E. Mueller, G. Solanes, and B. M. Spiegelman. 1998. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. J. Clin. Invest. 101: 1-9. Kinyamu H. K., and R. C. Ewan. 1994. Energy and protein metabolism of the Chinese pig. J. Anim. Sci. 72: 2068-2074. Kouba, M., M. Bonneau, and J. Noblet. 1999. Relative development of subcutaneous, intermuscular, and kidney fat in growing pigs with different body compositions. J. Anim. Sci. 77: 622-629. Kraemer, F. B., and W. J. Shen. 2002. Hormone-sensitive lipase control of intracellular tri-(di-) acylglycerol and cholesteryl ester hydrolysis. J. lipid Res. 43: 1585-1594. Kwiterovich Jr, P. O. 2000. The metabolic pathways of high-density lipoprotein, low-density lipoprotein, and triglycerides: a current review. Am. J. Cardiol. 86: 5-10. Le-Lay, S., I. Lefrere, I. C. Trautwein, I. Dugail, and S. Krief. 2002. Insulin and sterol-regulatory element-binding protein-1C (SREBP-1C) regulation of gene expression in 3T3-L1 adipocytes identification of CCAAT/enhancer-binding protein β as an SREBP-1C target. J. Biol. Chem. 277: 35625-35634. Lewis B. 1973. Classification of lipoproteins and lipoprotein disorders. J. Clin. Pathol. Clin. 5: 26-31. Li, B., N. Zerby, and K. Lee. 2007. Heart fatty acid binding protein is unregulated during porcine adipocyte development. J. Anim. Sci.85:1651-1659. Manini, T. M., B. C. Clark, M. A. Nalls, B. H. Goodpaster, L. L. Ploutz-Snyder, and T. B. Harris. 2007. Reduced physical activity increases intermuscular adipose tissue in healthy young adults. Am. J. Clin. Nutr. 85: 377-384. Mihara, K. 1990. Structure and regulation of rat liver microsomal stearoyl-CoA desaturase gene. J. Biochem. 108: 1022-1029. Minokoshi, Y., C. Toda, and S. Okamoto. 2012. Regulatory role of leptin in glucose and lipid metabolism in skeletal muscle. J. Endocr. Metab. 16: S562. Nygard, A. B., C. B. Jorgensen, S. Cirera, and M. Fredholm. 2007. Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Mol. Biol. 8: 67. Parraga, A., L. Bellsolell, A. R. Ferre-D'Amare, and S. K. Burley. 1998. Co-crystal structure of sterol regulatory element binding protein 1a at 2.3 A resolution. Structure 6: 661-672. Paton, C. M, and J. M. Ntambi, 2009. Biochemical and physiological function of stearoyl-CoA desaturase. Am. J. Physiol. Endocrinolo. Metab. 297: 28-37. Pelleymounter, M. A., M. J. Cullen, D. Healy, R. Hecht, D. Winters, and M. McCaleb. 1998. Efficacy of exogenous recombinant murine leptin in lean and obese 10-to 12-mo-old female CD-1 mice. Am. J. Physiol. Renal. Physiol. 275: R950-R959. Pethick D.W., G. S. Harper, and V. H. Oddy. 2004. Growth, development and nutritional manipulation of marbling in cattle: a review. Aust. J. Exp. Agric. 44: 705-715. Price, N. T., F. R. van der Leij, V. N. Jackson, C. G. Corstorphine, R. Thomson, A. Sorensen, and V. A. Zammit. 2002. A novel brain-expressed protein related to carnitine palmitoyltransferase I. Genomics 80: 433-442. Ramsay, T. G., X. Yan, and C. Morrison. 1998. The obesity gene in swine: sequence and expression of porcine leptin. J. Anim. Sci. 76: 484-490. Rydel, T. J., J. M. Williams, E. Krieger, F. Moshiri, W. C. Stallings, S. M. Brown, and M. F. Alibhai. 2003. The crystal structure, mutagenesis, and activity studies reveal that patatin is a lipid acyl hydrolase with a Ser-Asp catalytic dyad. Biochemistry 42: 6696-6708. SAS. 2013. SAS/STAT User's guide, Release 9.3 ed. Cary, NC, USA SAS Institute Inc. Sellier, P. 1998. Genetics of meat and carcass traits. Page 463. In: The Genetics of the Pig. Rothschild, M. F., and Ruvinsky, A. (ed.) CAB International, Wallingford, UK. Smirnova, E., E. B. Goldberg, K. S. Makarova, L. Lin, W. J. Brown, and C. L. Jackson. 2005. ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells. EMBO reports 7: 106-113. Smith, S. B., H. Kawachi, C. B. Choi, C. W. Choi, G. Wu, and J. E. Sawyer. 2009. Cellular regulation of bovine intramuscular adipose tissue development and composition. J. Anim. Sci. 87: 72-82. Stuart S., W. Andrzej, and J. K. Anik. 2003. Structural and functional organization of the animal fatty acid synthase. J. lipid Res. 42: 289-317. Suzuki, K., H. Kadowaki, T. Shibata, H. Uchida, and A. Nishida. 2005. Selection for daily gain, loin-eye area, backfat thickness and intramuscular fat based on desired gains over seven generations of Duroc pigs. Livest. Proc. Sci. 97: 193-202. Tyra, M., K. Ropka-Molik, A. Terman, K. Piorkowska, M. Oczkowicz, and A. Bereta. 2013. Association between subcutaneous and intramuscular fat content in porcine ham and loin depending on age, breed and FABP3 and LEPR genes transcript abundance. Mol. Biol. Rep. 40: 2301-2308. Van Laack, R. L., S. G. Stevens, and K. J. Stalder. 2001 The influence of ultimate pH and intramuscular fat content on pork tenderness and tenderization. J. Anim. Sci. 79: 392-397. van der Leij, F. R., K. B. Cox, V. N. Jackson, N. C. Huijkman, B. Bartelds, J. R. Kuipers, and N. T. Price. 2002. Structural and functional genomics of the CPT1B gene for muscle-type carnitine palmitoyltransferase I in mammals. J. Biol. Chem. 277: 26994-27005. Webber, J. 2003. Energy balance in obesity. Proc. Nutr. Soc. 62: 539-543. Weber, J. M., and F. Haman. 2004. Oxidative fuel selection: adjusting mix and flux to stay alive. Int. Congr. 1275: 22-31. Yamazaki, N., Y. Yamanaka, Y. Hashimoto, Y. Shinohara, A. Shima, and H. Terada. 1997. Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I. FEBS Lett. 409: 401-406. Young L. D. 1992. Comparison of Meishan, Fengjing, Minzhu and Duroc swine: effects on postweaning growth, feed efficiency, and carcass traits. J. Anim. Sci. 70: 2020-2029. Yokoyama, C., X. Wang, M. R. Briggs, A. Admon, J. Wu, X. Hua, and M. S. Brown. 1993. SREBP-1, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197. Zhang, Y., R. M. Proenca, M. Maffei, M. Barone, L. Leopold, and J. M. Friedman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425-432. Zhao, S. M., L. J. Ren, L. Chen, X. Zhang, M. L. Cheng, W. Z. Li, Y. Y. Zhang, and S. Z. Gao. 2009. Differential expression of lipid metabolism related genes in porcine muscle tissue leading to different intramuscular fat deposition. Lipids 44: 1029-1037. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5435 | - |
dc.description.abstract | 肉中的肌內脂肪能夠影響肉之柔嫩度(tenderness)、多汁性(juiciness)及風味(flavor),在進行屠宰時常發現,臺灣本土黑豬其肌內脂肪含量高於瘦肉型洋種豬,而前人研究中對於豬隻基因表現與肉質相關的研究則常著重在背最長肌。因此,本研究除了比較臺灣黑豬與洋種豬的背最長肌之脂質代謝相關基因表現差異,亦同時納入分析肩胛與後腿其脂質代謝基因表現,探討不同豬種以及不同肌肉間的肌內脂肪累積潛能。
本試驗豬隻包括來自行政院農委會高雄種畜繁殖場之14頭高畜黑豬,以及來自民間豬場之14頭洋種豬(包含7頭藍瑞斯與7頭杜洛克)與16頭黑豬;收集其屠體性狀資料(屠體重、屠體長、第一肋、最後肋及最後腰椎三點平均背脂厚度)。此外,採集其第10至11肋間背最長肌、肩胛半棘肌與後腿股直肌之肌肉樣本以抽取RNA,並以即時定量聚合酶(real-time PCR)SYBR法偵測其脂質代謝相關基因表現,包括ATGL、SCD、 HSL、LDLR、SREBP1c、CPT1b、FAS、Leptin 以及H-FABP。 試驗結果顯示,在高畜黑豬及民間黑豬的背最長肌及半棘肌中,其脂質生成相關基因SREBP1c及H-FABP的表現,顯著高於洋種豬(P<0.05),而其脂質氧化基因CPT1b顯著低於洋種豬(P<0.05),但高畜黑豬及民間黑豬兩者間沒有顯著差異;此外,洋種豬半棘肌之脂質分解相關基因Leptin的表現顯著高於高畜黑豬及民間黑豬,但兩種黑豬間則無顯著差異。在股直肌中,高畜黑豬與民間黑豬之脂質分解相關基因Leptin的表現顯著低於洋種豬(P<0.05),但高畜黑豬與民間黑豬兩者間沒有顯著差異;而民間黑豬其脂質生成相關基因SREBP1c顯著高於高畜黑豬及洋種豬(P<0.05),但高畜黑豬及洋種豬兩者間沒有顯著差異,脂肪酸轉運基因H-FABP表現於三者之股直肌中以民間黑豬最高,高畜黑豬次之,洋種豬最低(P<0.05)。 在不同肌肉部位的比較中,高畜黑豬中在股直肌之H-FABP、FAS與SREBP1c表現顯著低於其背最長肌及半棘肌(P<0.05),且在背最長肌及半棘肌之間並沒有顯著差異;然而,背最長肌與半棘肌的ATGL表現顯著高於股直肌(P<0.05),但在兩者間沒有顯著差異。洋種豬股直肌之ATGL、H-FABP及CPT1b顯著地低於背最長肌與半棘肌(P<0.05),而背最長肌與半棘肌之間則無顯著差異;背最長肌之FAS表現顯著高於其半棘肌及股直肌(P<0.05),但在半棘肌及股直肌兩者間則無差異,而SREBP1c的表現以背最長肌為最高,半棘肌次之,股直肌為最低(P<0.05);Leptin基因在股直肌的表現量高於半棘肌及背最長肌(P<0.05),但於半棘肌及背最長肌則無差異。民間黑豬之SREBP1c表現在股直肌中為最高(P<0.05),而在其背最長肌與半棘肌中則有相似的表現;半棘肌之H-FABP表現顯著高於背最長肌及股直肌(P<0.05),其在背最長肌與股直肌之間沒有差異;背最長肌之ATGL表現顯著高於半棘肌(P<0.05),但在其餘肌肉之間則無顯著差異。 綜上所述,高畜黑豬與民間黑豬在背最長肌、肩胛及後腿之肌內脂肪累積潛力高於洋種豬,而此三豬種之背最長肌與肩胛之肌內脂肪累積潛能應高於後腿。為確認表型與基因表現之間的關係,則需要更多資訊,如一般成分分析、肉質性狀分析等。 | zh_TW |
dc.description.abstract | Intramuscular fat (IMF) affects meat quality, especially juiciness, tenderness and flavor. The content of IMF are various in different types of muscle. Previous literature indicated that IMF content in M. longissimus muscle was higher in Kaohsiung Animal Propagation Station Black Pigs (KHAPS Black pigs) than in exotic pigs. However, the study in pig meat quality and phenotype of gene expression always focused on the longissimus muscle. Therefore, the objective of this study was to investigate the expression levels of lipid metabolism-related genes in different types of muscle among breeds. The study collected muscle samples from M. Longissimus Dorsi (MLD) between the 10th and 11th ribs, shoulder (M. Semispinalis Capitis, MSC) and ham (M. Rectus Femoris, MRF) of 14 KHAPS Black Pigs (K), 14 exotic pigs (E, seven Duroc and seven Landrace pigs) and 16 black pigs (B). Real-time PCR was used to quantitate mRNA expressions of the lipid metabolism-related gene, including ATGL, SCD, HSL, LDLR, SREBP1c, CPT1b, FAS, Leptin and H-FABP genes.
FAS is a key enzyme in fatty acid synthesis, and SREBP1c is the principal regulatory transcription factor for fatty acid synthesis in mammals. H-FABP is critical for fatty acid uptake as well as intracellular trafficking in muscles. Our results showed that the expression levels of SREBP1c and H-FABP in E pigs were the lowest of all (P<0.05), and were similar in K/B. However, the expression level of FAS was significantly higher in MSC of K than E (P<0.05) , which suggested that the lipid synthesis and fatty acid transportation were higher in K and B compared with E, indicated the MSC of K expected more fatty acid synthesis than E pigs. ATGL is present in all cell types that can start initial step in lipid hydrolysis. Unlike HSL, ATGL shows strong preference for triacylglycerol as substrate. In skeletal muscle, Leptin played a major role in anti-obesity by directly affecting glucose and fatty acid metabolism.The expression levels of ATGL and Leptin in E pigs were the highest of all (P<0.05),and were similar in K/B. In KHAPS Black Pigs, the expression levels of H-FABP, FAS, and SREBP1c were the lowest in MRF (P<0.05); but there were no significant difference between MLD and MSC. ATGL was significantly higher in MLD than in MSC (P<0.05); but there were no significant difference between MSC and MRF. In exotic pigs, the expression levels of ATGL, H-FABP and CPT1b in MRF were the lowest of all (P<0.05), and were similar in MLD/MSC. FAS in MLD was the highest of all (P<0.05), and there were similar in MSC/MRF. The expression levels of SREBP1c were in following order: MLD, MRF and MSC; MLD was the highest (P<0.05). Leptin was the highest in MRF (P<0.05), but not significantly difference between MLD and MSC. In black pigs, MRF showed the highest expression levels of SREBP1c (P<0.05), but was similar in MLD and MSC. The expression levels of H-FABP in MRF was the lowest of all (P<0.05), but there were no significant difference between MLD and MSC. ATGL was significantly higher in MLD than in MSC (P<0.05), but there were not significantly difference between MSC and MRF. In conclusion, high potential of IMF accumulation could be expected in loin,shoulder and ham in K and B than in comparison of the expressions of lipid metabolism-related genes to exotic pigs. However, We need more information like carcass trait to analysis the association between phenotype and mRNA expressions. | en |
dc.description.provenance | Made available in DSpace on 2021-05-15T17:58:48Z (GMT). No. of bitstreams: 1 ntu-103-R00626016-1.pdf: 1853328 bytes, checksum: ce49c8189f9b9ee9570f095f0962e89d (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 目錄
誌謝 I 目錄 II 圖次 IV 表次 V 中文摘要 VI AbstractVIII 壹、前言 1 貳、文獻檢討 3 一、臺灣本地豬種與發展進程 3 (一)臺灣本地豬種 3 (二)臺灣本土豬隻發展進程 6 (三)臺灣黑豬之肉質特性 7 二、肌內脂肪之介紹 10 (一)肌內脂肪與肉質之關聯性 10 (二)肌內脂肪累積與代謝 10 (三)肌內脂肪與豬種之關聯性 10 三、肌內脂質代謝相關基因 12 (一)脂質生成相關基因 12 (二)脂質運送相關基因 16 (三)脂質分解相關基因 19 (四)脂肪酸去飽和基因 27 四、以基因表現預測肌內脂肪累積潛能 28 参、材料與方法 30 一、試驗豬隻 30 二、部位採樣 30 三、屠宰資訊收集 30 四、即時定量聚合酶鏈鎖反應(real-time PCR)分析 35 (一)肌肉組織總RNA萃取 35 (二)反轉錄DNA製備 35 (三)引子設計 36 (四)SYBR Green法進行即時定量聚合酶鏈鎖反應 36 (五)標準曲線定量 36 (六)統計分析 36 肆、結果與討論 39 一、結果 39 (一)杜洛克與藍瑞斯脂質代謝相關基因表現比較 39 (二)屠體性狀表現差異分析 39 (三)不同豬種間其背最長肌(MLD)脂質代謝相關基因表現差異 42 (四)不同豬種間其肩胛半棘肌(MSC)脂質代謝相關基因表現差異 42 (五)不同豬種間其後腿股直肌(MRF)脂質代謝相關基因表現之差異 42 (六)豬種間不同部位肌肉脂質代謝相關基因表現之差異 43 二、討論 48 (一)脂質代謝相關基因表現與IMF累積研究探討 48 (二)MLD與MSC脂質相關代謝基因表現模式 50 (三)MRF脂質相關代謝基因表現模式 50 (四)不同豬種間脂質代謝相關基因表現之差異性 51 (五)屠體性狀與基因表現探討IMF累積潛力 51 伍、結論 58 陸、參考文獻 59 | |
dc.language.iso | zh-TW | |
dc.title | 豬隻不同品種及部位肌肉之脂質代謝相關基因表現 | zh_TW |
dc.title | Expression of lipid metabolism-related genes in different muscles and different breeds of pigs | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 羅玲玲(Ling-Ling Lo),林榮信(Rong-Shinn Lin),林正鏞(Cheng-Yung Lin) | |
dc.subject.keyword | 背最長肌,半棘肌,股直肌,肌內脂肪,脂質代謝相關基因,豬, | zh_TW |
dc.subject.keyword | intramuscular fat,Lipid metabolism-related genes,M. Longissimus Dorsi,M. Semispinalis Capitis,M. Rectus Femoris,pig, | en |
dc.relation.page | 65 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2014-02-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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