請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54080
標題: | 北冬蟲夏草及茶蠶砂之化學成分分析研究 Chemical investigation of Cordyceps militaris and Andraca theae droppings |
作者: | Tzu-Yun Chou 周姿妘 |
指導教授: | 張嘉銓 |
共同指導教授: | 李水盛 |
關鍵字: | 北蟲草,茶蠶砂, Cordyceps militaris,Andraca theae droppings, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 本論文包含兩部分: 第一部分:北冬蟲夏草之成分分析之研究 北冬蟲夏草(Cordyceps militaris)是使用多年的中草藥具有許多活性,例如:抗發炎、抗糖尿病、抗腫瘤等功效。由於天然的北冬蟲夏草的來源不足,因此人工培養之北蟲草為目前主要的生產方式。本研究探討以白米(CM-A)、白米添加3%茶葉(CM-B)以及白米添加3%茶蠶砂(CM-C)等三種培養基培養出北冬蟲夏草其活性成分的差異。取白米培養得到的蟲草子實體乙醇抽取物,極性劃分成氯仿、乙酸乙酯、正丁醇和水可溶部分及沉澱物,氯仿及正丁醇可溶部分經Sephadex LH-20、半製備HPLC等管柱層析法於得到1個新化合物及9個已知結構,分別為phenylalanine (1)、cordycedipeptide B (2)、nicotinic acid (3)、tryptophan (4)、N6-(2-hydroxyethyl)-adenosine (5)、uracil (6)、uridine (7)、cordycepin (8)、ergosterol (9) 和 mannitol (10)。其中化合物2為首次於天然物中分離,為含有ornithine骨架的類雙肽新化合物。大部分所分離到的化合物與天然蟲草的成分是相似的;化合物5並未自此子實體中被發現,且蟲草素(cordycepin)含量比天然的蟲草多(125倍)。以白米培養之北蟲草子實體成分與另兩種培養基培養的比較,3 %之茶葉添加物在化合物5、8及9含量上無顯著影響;3 %茶蠶砂添加物所培養的子實體中並無發現化合物9,但化合物5的含量增加2~3倍。這些培養的北蟲草子實體因培養的方式不同而有成分上的改變,可以進一步作為增加特定活性成分的方法。 第二部分:茶蠶砂之成分分析之研究 蠶砂為蠶排泄物,具有促進傷口癒合、抗腫瘤、降血糖及血脂等功能。茶蠶(Andraca theae)為危害茶樹的害蟲。茶蠶砂為其排泄物,其化學成分尚未有任何報告,其可能用途亦未有報告。基於上述原因本研究以層析法分離茶蠶砂之化學成分,釐清與茶葉組成的差異,進而評估茶蠶砂開發為藥用的潛力。 茶蠶砂的乙醇萃取物經極性劃分成氯仿、乙酸乙酯、正丁醇和水可溶部分,乙酸乙酯可溶部分利用Sephadex LH-20、CPC、Lobar RP-18和半製備HPLC等管柱分離得到1個生物鹼(11)、3個羧酸類(12、13及15)及10個黃酮類(14與16-24)。黃酮類化合物16、17及19-24之總含量比茶葉多4.2倍,所以茶蠶這些化合物可能無法為所利用,或經其腸道細菌轉化而排出,化合物11並未見於茶葉成分,是茶蠶代謝咖啡因的產物。故此茶蠶砂可成為提供前述黃酮類活性成分的重要來源。 This dissertation includes two parts: Part 1. Chemical investigation of the Cordyceps militaris Cordyceps militaris (L.) Link is a traditional Chinese medicine, with various biological activities, including anti-inflammation, anti-tumor, and anti-diabetes. Because of its limited natural supply, culture of C. militaris has become the major source. The aim is to discuss the active ingredients of the cultured C. militaris which was cultured with different sources such as rice (CM-A), rice plus 3 % tea leaves (CM-B) and rice plus 3 % feces of Andraca bipunctata (CM-C). The ethanolic extract of dried C. militaris (cultured with rice) was divided into fractions soluble in CHCl3, EtOAc, n-BuOH and a precipitate via liquid-liquid partitioning process. The n-BuOH- and CHCl3- soluble fractions were separated by chromatographic methods to afford ten compounds (1-10). Through NMR and MS spectroscopic analyse, they were characterized as phenylalanine (1), cordycedipeptide B (2), nicotinic acid (3), tryptophan (4), N6-(2-hydroxyethyl)-adenosine (5), uracil (6), uridine (7), cordycepin (8), ergosterol (9), and mannitol (10). Of that cordycedipeptide B (2) is a new compound. The chemical components from cultured C. militaris are found to be similar to those from the natural one. Among them, N6-(2-hydroxyethyl)-adenosine (5) has never been identified from this species, and the content of cordycepin from the cultured is 125 folds more than that from the nature. This study indicates that the ingredients of CM-B has no significant difference from those of CM-A. Nevertheless, the amount of N6-(2-hydroxyethyl)-adenosine (5) in CM-C is increased 2~3 folds. With different culture conditions, the compounds of C. militaris are not the same. Hence, the culture conditions can be used as a tool to produce specific active compound(s). Part 2. Chemical investigation of the Andraca theae droppings. Silkworm Feculae (droppings of silkworm), a traditional Chinese medicine possess to a number of bioactivities, including promotion of wound healing, anti-tumor, anti-hyperglycemia, and anti-hyperlipidemia. Tea silkworm (Andraca theae) is a common tea pestsin, and its droppings have not reported yet, nor chemical constitute of its medical use. For the above reasons, the present study was aimed to isolate the chemical constituents of tea silkworm feces by chromatographic methods, and evaluate its potential for medical uses. The ethanolic extract of the dried droppings of tea silkworm was divided into fractions soluble in CHCl3, EtOAc, n-BuOH, and a precipitate via liquid-liquid partitioning process. The EtOAc-soluble fractions was separated by chromatographic methods including Sephadex LH-20, CPC, and RP-18 column to afford 14 compounds (11-24). They were characterized as one xanthine alkaloid (11), three benzoic acids (12, 13 and 15) and ten flavonoids (14, 16-24). 1,7-Dimethyl xanthine (11) is not present in tea leaves. The amount of compounds 16, 17, and 19-24 in the feces is about 4.2 folds related to that of tea droppings. Thus, these compounds are not absorted and eliminated directly, except for 11, which might be transformed from caffine by microflora of the insect. This study suggest the tea silkworm droppings to be a good source of the bioactive flavonoids from tea leaves. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54080 |
全文授權: | 有償授權 |
顯示於系所單位: | 藥學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 13.78 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。