化學氣相沉積功能性聚對二甲苯鍍膜用於製備抗垢基材

Abstract

細胞與生物材料相互作用是許多醫療設備，如血液接觸的設備，生物傳感器和植入物的性能表現的關鍵。生物材料的表面改質是一項常見的戰略，用以控制材料和生物系統間的界面交互作用。可應用到大多數材料的簡易表面改質技術是被高度期望的。藉由化學氣相沉積(Chemical Vapor Deposition ,CVD)的功能性聚對二甲苯超薄層沉積可以適用於各種各樣的基材。 在這項研究中，我們製備炔官能基塗層並且經由點擊化學接上含疊氮化物（Az）的分子。合成被接枝的疊氮分子聚合物，然後藉由點擊化學固定在CVD-炔基基材上。我們發現所沉積的聚合物層是穩定的，並能調節細胞間的相互作用。P(SBMA)與PEGMA可以抗蛋白質吸附和細胞貼附，而PNIPAAm可以藉由其熱敏感反應特性，製作細胞薄片(cell sheet)。我們指出在CVD-炔基基材是一個很好的平台製造功能性表面去調節細胞與材料間交互作用。 在第二部分中，我們利用開環聚合的方式在CVD-OH表面上製造高度分支化聚丙三醇的抗髒污表面。此外，我們還利用開環聚合的方式製造具有尾端烯基的反應性表面。接著我們使用硫醇基分子形成特異性粘附表面，如RGD-SH和生物素-PEG-SH。高度分支化聚丙三醇表面與具有烯基官能基的表面還提供了抗細胞的背景，同時提供烯基可聚合其它分子。我們已經證明在表面可用與RGD-SH的結合以增強細胞附著和生物素-PEG-SH的專一性抗生物素蛋白- FITC結合。我們的方法提供了一個簡單的工具來製作具有可以控細胞親和性的表面。

Cell-biomaterial interaction is critical for the performance of many medical devices such as blood-contacting devices, biosensors and implants. Surface modification of biomaterials is a common strategy to control interfacial interactions between materials and biological systems. A facile surface modification technique that could be applied to most materials is highly desirable. Deposition of an ultra-thin layer of functional poly-(p-xylylenes) via chemical-vapor-deposition of [2.2]paracyclophanes could be applied to a wide variety of substrates. In this study, we prepared an alkyne-functionalized coating on which azide (Az)-containing molecules could be conjugated via click chemistry. Several polymers that are grafted with azido molecules were synthesized and then immobilized on the CVD-alkyne substrates via click chemistry. We found that the deposited polymer layers were stable and could modulate cell interactions. The non-fouling P(SBMA) and PEGMA resist protein adsorption and cell adhesion, while the PNIPAAm substrate could be used to fabricate cell sheets by its thermo-responsive property. We demonstrate that the CVD-alkyne substrate is a good platform for the fabrication of functional surfaces in order to modulate of cell-material interactions. In the second part, we create a nonfouling surface by hyperbranched polyglycerol ring-opening polymerization on the CVD-OH coated surface. Moreover, we also create a reactive surface by the ring-opening polymerization which provide terminal alkenyl group. Then we use the molecules with thiol group, RGD-SH and biotin-PEG-SH, to form specific adhesion surface. Furthermore, hyperbranched polyglycerol surface with functional alkenyl group provided a cell-resisting background surface, at the same time providing accessible functional alkenyl group can be conjugated to the other molecules. We have demonstrated that the surface can be used for the binding of RGD-SH to enhance the cell attachment and the binding of biotin-PEG-SH to the specific binding of avidin-FITC. Our polymerization method provides a simple tool to fabricate surfaces with controllable cell affinity.