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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳賢明(Hieng-Ming Ting) | |
dc.contributor.author | Yu-Cheng Chen | en |
dc.contributor.author | 陳鈺澄 | zh_TW |
dc.date.accessioned | 2021-06-15T16:50:22Z | - |
dc.date.available | 2020-08-24 | |
dc.date.copyright | 2020-08-24 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-18 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53197 | - |
dc.description.abstract | 3-butenenitrile (3BN) 是一種腈類化合物,其主要是植物在防禦中產生的次級代謝物硫代葡萄糖苷所水解而成。它可以觸發阿拉伯芥中防禦相關的信號途徑來對抗生物逆境。2.5 mM 3BN處理可使阿拉伯芥Col-0有細胞死亡的狀況,而這個過程應該是藉由一氧化氮所調控。在前人微矩陣資料以及GC-MS的代謝圖譜分析當中可知,在阿拉伯芥Col-0 處理2.5 mM 3BN可誘導植物中的防禦相關基因、單醣代謝相關基因的表現,並改變初級代謝物的含量。其中這些初級代謝物在植物防禦的調控上扮演了相當重要的角色,故也觀察阿拉伯芥在3BN處理後是否提升植物防禦的能力。而在Col-0處理2.0 mM 3BN後,植物中防禦相關的賀爾蒙水楊酸以及茉莉酸含量都有明顯上升的狀況,且沒有細胞死亡的狀況發生。如果進一步對在硝酸還原酶的突變株 (nia1nia2) 去進行2.0 mM 3BN處理,可發現茉莉酸含量相較於Col-0來說上升幅度更為明顯,而在水楊酸含量上升幅度則較小。而在前述實驗的基礎上進行壞死性病原菌 (Pectobacterium carotovorum subsp. carotovorum 以及 Botrytis cinerea) 接種,可發現Col-0處理2.0 mM 3BN後可提升植物對病原菌的抗性,而在nia1nia2中沒有看見抗性上升的狀況。此外,對其接種常見的啃食性昆蟲秋行軍蟲,可發現不論是Col-0或是nia1nia2處理2.0 mM 3BN後,植物對秋行軍蟲的抗性都有所提升。總體而言,上述結果表明,3BN可影響阿拉伯芥中的防禦反應,值得更進一步研究。 | zh_TW |
dc.description.abstract | 3-butenenitrile (3BN) is a type of nitrile and is one of the glucosinolate hydrolysis products which can activate defense responses in Arabidopsis thaliana to biotic stresses. A 2.5 mM 3BN treatment can cause cell death in Arabidopsis, which is regulated by nitric oxide. In previous studies, 2.5 mM 3BN treatment induces defense response genes and genes involved in monosaccharide metabolic process in wild type Arabidopsis. Furthermore, the same treatment up-regulated primary metabolites, which are known to be crucial components in modulating plant defense responses. We further investigate the defense response in Arabidopsis upon 3BN treatment. After 2.0 mM 3BN treatment, the amount of defense hormones, jasmonic acid (JA) and salicylic acid (SA), in Arabidopsis were increased, and the treatment did not lead to cell death. In addition, treatment with 2.0 mM 3BN induced the production of JA and SA in the nitrate reductase loss-of-function mutant (nia1nia2), which intrinsically has higher JA content but lower SA content. We also inoculated necrotrophic pathogen on Arabidopsis treated with 2.0 mM 3BN.The treatment improved plant tolerance to the necrotrophic pathogen (Pectobacterium carotovorum subsp. carotovorum and Botrytis cinerea) in Col-0 but not in nia1nia2. Moreover, 2.0 mM 3BN treatment enhanced plant resistance toward chewing insect Spodoptera frugiperda. Overall, these results suggest that 3BN influence plant defense responses in Arabidopsis, which warrants further investigation. | en |
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dc.description.tableofcontents | 口試審定書 i 謝誌 ii 摘要 iii Abstract iv 常用縮寫與全名對照表 v 目錄 vii 圖目錄 x 附錄目錄 xi 第一章、前言 1 1. 植物病蟲害防禦機制 1 1.1. 植物免疫反應 2 1.2. DAMPs以及DTI反應 3 1.3. 植物防禦相關信號 4 2. 植物次級代謝物 8 2.1. 硫代葡萄糖苷 (glucosinolate) 9 2.2. 異硫氰酸酯 (isothiocyanate) 9 2.3. 腈 (nitrile) 10 3. 前人研究與研究動機 11 第二章、材料與方法 14 1. 植物材料與栽種條件 14 2. 植物快速DNA萃取 14 3. 聚合酶連鎖反應 15 4. DNA瓊脂糖凝膠電泳 16 5. 轉錄分析表現 16 5.1. 植物RNA萃取 16 5.2. 反轉錄聚合酶連鎖反應 17 5.3. RT-PCR 18 6. 3BN處理後性狀測定 19 6.1. 3BN treatment 19 6.2. 電解質滲透率 (ion leakage assay) 19 6.3. 葉綠素測定 20 6.4. ROS測定 20 7. MAPK磷酸化試驗 21 7.1. 3BN誘導MAPK磷酸化測定 21 7.2. 蛋白質抽取 21 7.3. 蛋白質定量 22 7.4. 蛋白質電泳 22 7.5. Commassie blue 染色 24 7.6. 西方墨點法 25 7.7. Ponceau S染色 26 8. 生物性逆境測試 27 8.1. 細菌性軟腐病菌接種測試 27 8.2. 真菌性灰黴病接種測試 28 8.3. 秋行軍蟲蟲害測試 29 9. 植物賀爾蒙萃取 29 第三章、結果 31 1. 3BN處理後阿拉伯芥的反應 31 2. 3BN處理後對MAPK信號傳遞途徑的影響 32 3. 3BN處理可誘導植物抗病相關基因的表現 32 4. 3BN處理可提升植物中防禦賀爾蒙的產量 33 5. 3BN處理後阿拉伯芥對於壞死性病原菌以及啃食性昆蟲反應之比較 33 四、討論 35 1. 3BN處理可藉由NO來調控植物的細胞死亡 35 2. 3BN處理後可能參與的信號傳遞途徑 36 3. 3BN處理後植株中代謝物改變和植物細胞死亡以及防禦反應之間的關係 37 3.1 單醣以及TCA cycle相關有機酸 39 3.2 胺基酸 41 3.3 其它相關代謝物 43 4. 阿拉伯芥處理3BN後對防禦反應之影響 44 4.1. 3BN處理可誘導阿拉伯芥Col-0對壞死性病原菌Pcc及Bc的抗性 44 4.2. 3BN處理無法誘導NO突變株nia1nia2對壞死性病原菌Pcc及Bc的抗性 45 4.3. 3BN處理可誘導Col-0以及nia1nia2對啃食性昆蟲秋行軍蟲的抗性 47 5. 結語 48 五、參考文獻 50 | |
dc.language.iso | zh-TW | |
dc.title | 3-butenenitrile在阿拉伯芥中誘導植物防禦及信號傳遞之研究 | zh_TW |
dc.title | The study of signal transduction of 3-butenenitrile-induced plant defense in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李金美(Chin-Mei Lee),楊淑怡(Shu-Yi Yang),蔡皇龍(Huang-Lung Tsai),曾怡潔(I-Chieh Tseng) | |
dc.subject.keyword | 3-butenenitrile,初級代謝,一氧化氮,DAMPs,植物防禦反應, | zh_TW |
dc.subject.keyword | 3-butenenitrile,primary metabolite,Nitric Oxide,DAMPs,plant defense response, | en |
dc.relation.page | 84 | |
dc.identifier.doi | 10.6342/NTU202002439 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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