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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 劉嚞睿(Je-Ruei Liu) | |
dc.contributor.author | Yu-Han Chiu | en |
dc.contributor.author | 邱昱翰 | zh_TW |
dc.date.accessioned | 2021-06-15T16:46:14Z | - |
dc.date.available | 2022-08-19 | |
dc.date.copyright | 2020-09-22 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-16 | |
dc.identifier.citation | 行政院衛生福利部統計處。2019。107年死因統計結果分析。 行政院衛生福利部統計處。2019。105-107年國人三高盛行率分析。 行政院衛生福利部國民健康局。2007。20歲以上成人高血脂判定標準。 行政院衛生福利部食品藥物管理署。2017。可供食品使用原料彙整一覽表。 Adams M. R., and Nicolaides L. 1997. Review of the sensitivity of different foodborne pathogens to fermentation. Food Control. 8(5-6): 227-239. Apostolou E., Kirjavainen P. V., Saxelin M., Rautelin H., Valtonen V., Salminen S. J., and Ouwehand A. C. 2001. Good adhesion properties of probiotics: a potential risk for bacteremia? FEMS Immunology and Medical Microbiology. 31(1): 35-39. Begley M., Hill C. and Gahan C. G., 2006. Bile salt activity in probiotics. Applied and Environmental Microbiology. 72(3): 1729-1738. Betters J. L., and Yu L. 2010. NPC1L1 and cholesterol transport. FEBS letters. 584(13): 2740-2747. Biggerstaff K. D., and Wooten J. S. 2004. Understanding lipoproteins as transporters of cholesterol and other lipids. Advances in Physiology Education. 28(3): 105-106. Bourrie B. C. Willing B. P., and Cotter P. D. 2016. The microbiota and health promoting characteristics of the fermented beverage kefir. Frontiers in Microbiology. 7: 647. Caggia C., De Angelis M., Pitino I., Pino A., and Randazzo C. L. 2015. Probiotic features of Lactobacillus strains isolated from Ragusano and Pecorino Siciliano cheeses. Food Microbiology. 50: 109-117. Charteris W. P., Kelly P. M., Morelli L., and Collins J. K. 1998. Antibiotic susceptibility of potentially probiotic Lactobacillus species. Journal of Food Protection. 61(12): 1636-1643. Chen P., Chen X., and Zhang S. 2019. Current status of familial hypercholesterolemia in China: a need for patient FH registry systems. Frontiers in Physiology. 10: 280. Hindler J. A., Humphries R. M., Richter S. S., Jorgensen J. H., Bernard K., Killian S. B., Bodeis-Jones S., Kohner P., Castanheira M., Matuschek E., Citron D. M., McDermott P., Couturier M. R., Patel S., and Fritsche T. R. 2015. Clinical and Laboratory Standards Institute. M45 Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. Wayne, PA, USA. Coppola R., Succi M., Tremonte P., Reale A., Salzano G., and Sorrentino E. 2005. Antibiotic susceptibility of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. Le Lait. 85(3): 193-204. De Smet I., Van Hoorde L., Vande Woestyne M., Christiaens H., and Verstraete W. 1995. Significance of bile salt hydrolytic activities of lactobacilli. Journal of Applied Bacteriology. 79(3): 292-301. Dashkevicz M. P., and Feighner S. D. 1989. Development of a differential medium for bile salt hydrolase-active Lactobacillus spp. Applied and Environmental Microbiology. 55(1): 11-16. Davis H. R., and Veltri E. P. 2007. Zetia: inhibition of Niemann-Pick C1 Like 1 (NPC1L1) to reduce intestinal cholesterol absorption and treat hyperlipidemia. Journal of Atherosclerosis and Thrombosis. 14(3): 99-108. During A., Dawson H. D., and Harrison, E. H. 2005. Carotenoid transport is decreased and expression of the lipid transporters SR-BI, NPC1L1, and ABCA1 is downregulated in Caco-2 cells treated with ezetimibe. The Journal of Nutrition. 135(10): 2305-2312. Dobson A., O'Sullivan O., Cotter P. D., Ross P., and Hill C. 2011. High-throughput sequence-based analysis of the bacterial composition of kefir and an associated kefir grain. FEMS Microbiology Letters. 320(1): 56-62. Durrington P. 2003. Dyslipidaemia. The Lancet. 362(9385): 717-731. Filipic B., Golic N., Jovcic B., Tolinacki M., Bay D. C., Turner R. J., Antic-Stankovic J, Kojic M., and Topisirovic L. 2013. The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. Research in Microbiology. 164(1): 46-54. Garcia-Calvo M., Lisnock J., Bull H. G., Hawes B. E., Burnett D. A., Braun M. P., Crona J. H., Davis H. R., Dean D. C., Detmers P. A., Graziano M. P., Hughes M., Maclntyre D. E., Ogawa A., O’Neill K. A., Iyer S. P. N., Shevell D. E., Smith M. M., Tang Y. S., Makarawicz A. M., Ujjainwalla F., Altmann S. W., Chapman K. T., and Thornberry N. A. 2005. The target of ezetimibe is Niemann-Pick C1-Like 1 (NPC1L1). Proceedings of the National Academy of Sciences. 102(23): 8132-8137. Garofalo C., Osimani A., Milanović V., Aquilanti L., De Filippis F., Stellato G., Di Mauro S., Turchetti B., Buzzini P., Ercolini D., and Clementi F. 2015. Bacteria and yeast microbiota in milk kefir grains from different Italian regions. Food Microbiology. 49: 123-133. Groisillier A., and Lonvaud-Funel A. 1999. Comparison of partial malolactic enzyme gene sequences for phylogenetic analysis of some lactic acid bacteria species and relationships with the malic enzyme. International Journal of Systematic and Evolutionary Microbiology. 49(4): 1417-1428. Harrigan W. F., and McCance M. E. 1966. Laboratory Methods in Microbiology. Academic Press. London and New York. 51. Heitzer T., Ylä-Herttuala S., Luoma J., Kurz S., Münzel T., Just H., Olschewski M., and Drexler H. 1996. Cigarette smoking potentiates endothelial dysfunction of forearm resistance vessels in patients with hypercholesterolemia: role of oxidized LDL. Circulation. 93(7): 1346-1353. Hobbs H. H., Brown M. S., and Goldstein J. L. 1992. Molecular genetics of the LDL receptor gene in familial hypercholesterolemia. Human Mutation. 1(6): 445-466. Hörnberg E., Ylitalo E. B., Crnalic S., Antti H., Stattin P., Widmark A., Bergh H., and Wikström P. 2011. Expression of androgen receptor splice variants in prostate cancer bone metastases is associated with castration-resistance and short survival. PLOS ONE. 6(4). Huang Y., Le J., Quan G., Wang X., Yang L., and Zhong L. 2014. Lactobacillus acidophilus ATCC 4356 prevents atherosclerosis via inhibition of intestinal cholesterol absorption in apolipoprotein E-knockout mice. Applied and Environmental Microbiology. 80(24): 7496-7504. Huang, Y., Wang, X., Wang, J., Wu, F., Sui, Y., Yang, L., and Wang, Z. 2013. Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. Journal of Dairy Science. 96(5): 2746-2753. Huang Y., Wu F., Wang X., Sui Y., Yang L., and Wang J. 2013. Characterization of Lactobacillus plantarum Lp27 isolated from Tibetan kefir grains: a potential probiotic bacterium with cholesterol-lowering effects. Journal of Dairy Science. 96(5): 2816-2825. Huang Y., and Zheng Y. 2010. The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells. British Journal of Nutrition. 103(4): 473-478. Hui D. Y., and Howles P. N. 2005. Molecular mechanisms of cholesterol absorption and transport in the intestine. Seminars in Cell and Developmental Biology. Academic Press. 16(2): 183-192. Igel M., Sudhop T., and von Bergmann K. 2002. Pharmacology of 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase inhibitors (statins), including rosuvastatin and pitavastatin. The Journal of Clinical Pharmacology. 42(8): 835-845. Iqbal J., and Hussain M. M. 2009. Intestinal lipid absorption. American Journal of Physiology-Endocrinology and Metabolism. 296: E1183-1194. Jones B. V., Begley M., Hill C., Gahan C. G., and Marchesi J. R. 2008. Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome. Proceedings of the National Academy of Sciences. 105(36): 13580-13585. Jones M. L., Tomaro-Duchesneau C., Martoni C. J., and Prakash S. 2013. Cholesterol lowering with bile salt hydrolase-active probiotic bacteria, mechanism of action, clinical evidence, and future direction for heart health applications. Expert Opinion on Biological Therapy. 13(5): 631-642. Katz S. E. 2016. Wild fermentation: The flavor, nutrition, and craft of live-culture foods. Chelsea Green Publishing. 202-203. Kielar D., Kaminski W. E., Liebisch G., Piehler A., Wenzel J. J., Möhle C. Heimer S., Langmann T., Friedrich S. O., Böttcher A., Barlage S., Drobnikand W., and Schmitz G. 2003. Adenosine triphosphate binding cassette (ABC) transporters are expressed and regulated during terminal keratinocyte differentiation: a potential role for ABCA7 in epidermal lipid reorganization. Journal of Investigative Dermatology. 121(3): 465-474. Kurniasih I. N., Liang H., Mohr P. C., Khot G., Rabe J. P., and Mohr A. 2015. Nile red dye in aqueous surfactant and micellar solution. Langmuir. 31(9): 2639-2648. Le B., and Yang S. H. 2018. Identification of a novel potential probiotic Lactobacillus plantarum FB003 isolated from salted-fermented shrimp and its effect on cholesterol absorption by regulation of NPC1L1 and PPARα. Probiotics and Antimicrobial Proteins. 11(3): 785-793. Le B., and Yang S. H. 2019. Effect of potential probiotic Leuconostoc mesenteroides FB111 in prevention of cholesterol absorption by modulating NPC1L1/PPARα/SREBP-2 pathways in epithelial Caco-2 cells. International Microbiology. 22(2): 279-287. Lee Y., Ho P. S., Low C. S., Arvilommi H., and Salminen S. 2004. Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut. Applied and environmental microbiology. 70(2): 670-674. Lim H. J., Kim S. Y., and Lee, W. K. 2004. Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use. Journal of Veterinary Science. 5(4): 391-395. Lim F. T., Lim S. M., and Ramasamy K. 2017. Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 assimilate cholesterol and modulate ABCA1, CD36, NPC1L1 and SCARB1 in vitro. Beneficial microbes. 8(1): 97-109. Lin C., Zhao J., and Jiang R. 2008. Nile red probing for the micelle-to-vesicle transition of AOT in aqueous solution. Chemical Physics Letters. 464(1-3): 77-81. Ma C., Zhang S., Lu J., Zhang C., Pang X., and Lv, J. 2019. Screening for cholesterol-lowering probiotics from lactic acid bacteria isolated from corn silage based on three hypothesized pathways. International Journal of Molecular Sciences. 20(9): 2073. Maiti N. C., Krishna M. M. G., Britto P. J., and Periasamy N. 1997. Fluorescence dynamics of dye probes in micelles. The Journal of Physical Chemistry B. 101(51): 11051-11060. Michael D. R., Davis T. S., Moss J. W. E. Calvente D. L., Ramji D. P., Marchesi J. R. Pechlivanis A, Plummer S. F., and Hughes T. R. 2017. The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice. Scientific Reports. 7(1): 2883. Michael D. R., Moss J. W., Calvente D. L., Garaiova I., Plummer S. F., and Ramji D. P. 2016. Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes. Beneficial Microbes. 7(3): 443-451. Moser S. A., and Savage D. C. 2001. Bile salt hydrolase activity and resistance to toxicity of conjugated bile salts are unrelated properties in lactobacilli. Applied and Environmental Microbiology. 67(8): 3476-3480. Murphy A., Faria-Neto J. R., Al-Rasadi K., Blom D., Catapano A., Cuevas A., Lopez-Jimenez F, Perel P, Santos R, Sniderman A, Sy R, Watts G. F., Zhao D, Yusuf S., and Wood D. 2017. World heart federation cholesterol roadmap. Global Heart. 12(3): 179-197. Nyanzi R., Jooste P. J., Cameron M., and Witthuhn C. 2013. Comparison of rpoA and pheS gene sequencing to 16S rRNA gene sequencing in identification and phylogenetic analysis of LAB from probiotic food products and supplements. Food Biotechnology. 27(4): 303-327. Owen S. C., Chan D. P., and Shoichet M. S. 2012. Polymeric micelle stability. Nano today. 7(1): 53-65. Rader D. J., Cohen J., and Hobbs H. H. 2003. Monogenic hypercholesterolemia: new insights in pathogenesis and treatment. The Journal of Clinical Investigation. 111(12): 1795-1803. Reboul E., Goncalves A., Comera C., Bott R., Nowicki M., Landrier J. F., ourdheuil-Rahmani D., Dufour C., Collet X., and Borel P. 2011. Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. Molecular Nutrition and Food Research. 55(5): 691-702. Rosa D. D., Dias M. M., Grześkowiak Ł. M., Reis S. A., Conceição L. L., and Maria do Carmo G. P. 2017. Milk kefir: nutritional, microbiological and health benefits. Nutrition Research Reviews. 30(1): 82-96. Sedláčková, P., Horáčková, Š., Shi, T., Kosova, M., and Plocková, M. 2015. Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains. Czech Journal of Food Sciences. 33(1): 13-18. Sharma M., Dangi P., and Choudhary M. 2014. Actinomycetes: source, identification, and their applications. International Journal of Current Microbiology Applied Sciences. 3(2): 801-832. Shehata M. G., El-Sahn M. A., El Sohaimy S. A., and Youssef M. M. 2019. In vitro assessment of hypocholesterolemic activity of Lactococcus lactis subsp. lactis. Bulletin of the National Research Centre. 43(1): 60. Shin H. S., Park S. Y., Lee D. K., Kim S. A., An H. M., Kim J. R., Kim M. J., Cha M. G., Lee S. W., Lim K. J., Lee K. O., and Ha N. J. 2010. Hypocholesterolemic effect of sonication-killed Bifidobacterium longum isolated from healthy adult Koreans in high cholesterol fed rats. Archives of Pharmacal Research. 33(9): 1425-1431. Tunçer S., and Banerjee S. 2017. Determination of autophagy in the Caco-2 spontaneously differentiating model of intestinal epithelial cells. Autophagy in Differentiation and Tissue Maintenance. Humana Press. New York. 55-70. Vidal R., Hernandez-Vallejo S., Pauquai T., Texier O., Rousset M., Chambaz J., Demignot S., and Lacorte J. M. 2005. Apple procyanidins decrease cholesterol esterification and lipoprotein secretion in Caco-2/TC7 enterocytes. Journal of Lipid Research. 46(2): 258-268. Yeo S., Lee S., Park H., Shin H., Holzapfel W., and Huh C. S. 2016. Development of putative probiotics as feed additives: validation in a porcine-specific gastrointestinal tract model. Applied Microbiology and Biotechnology. 100(23): 10043-10054. Yilmaz, M., Soran, H., and Beyatli, Y. 2006. Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiological Research. 161(2): 127-131. Yoon H. S., Ju J. H., Kim H. N., Park H. J., Ji Y., Lee J. E., Shin H. K., Do M. S., and Holzapfel W. 2013. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods. International Journal of Food Sciences and Nutrition. 64(1): 44-52. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53131 | - |
dc.description.abstract | 高膽固醇血症是導致心血管疾病的關鍵因素之一,家族性高膽固醇血症的低診斷率,加上不正常的飲食與運動習慣,使高膽固醇血症成為現代一大問題。已知特定的益生菌菌株具有降低腸道和血漿中膽固醇濃度的能力,益生菌可藉由膽鹽水解酶的活性和降低與膽固醇代謝相關的基因(例如NPC1L1,ABCG5,ABCG8和HMGCR)表現來降低腸細胞的膽固醇吸收,故益生菌可能是一種預防高膽固醇血症與心血管疾病的新方法。本研究的目的是以Caco-2細胞模式篩選具有降低膽固醇吸收能力的乳酸菌株,從克弗爾粒中分離出總共29株乳酸菌株,篩選出對Caco-2腸上皮細胞無細胞毒性的菌株,並評估其降低膽固醇吸收的能力。並以即時定量聚合酶連鎖反應進一步定量Caco-2細胞中與膽固醇代謝相關的基因之表現量。結果顯示Lactococcus lactis subsp. lactis YPK20和YPK23可以降低Caco-2細胞對膽固醇的吸收,並非透過細菌的膽鹽水解酶活性或細菌吸附膽固醇微胞的能力。以Lc. lactis subsp. lactis YPK20和YPK23預處理細胞,可以下調Caco-2細胞中NPC1L1基因的表達,而YPK23也可以降低Caco-2細胞中HMGCR基因的表現。此外,此二分離菌株在pH3環境下具有耐酸性,菌株代謝物具有抗病原菌活性。總的來說,本研究建構了一個檢測Caco-2細胞吸收膽固醇的模型,並發現Lc. lactis subsp. lactis YPK20和YPK23具有調節腸道上皮細胞基因表達和降低膽固醇吸收的潛在能力。 | zh_TW |
dc.description.abstract | Hypercholesterolaemia is one of the critical factors that cause cardiovascular disease (CVD). The low diagnosis of familial cholesterolaemia, abnormal diet, and exercise habits make cholesterolaemia become a big problem in modern life. It was reported that specific strains of probiotics had abilities to lower intestinal and plasma cholesterol levels, and supposed that probiotics would be a novel method to prevent hypercholesterolaemia and CVD. Probiotics lower cholesterol absorption of enterocytes by the activity of bile salt hydrolase (BSH) and down-regulation of the gene related to cholesterol metabolism, such as NPC1L1, ABCG5, ABCG8 and HMGCR. The aim of this study was to screen lactic acid bacterial strains with abilities to reduce cholesterol absorption in Caco-2 cells. A total of 29 lactic acid bacterial strains were isolated from kefir grains, and the strains without cytotoxicity to Caco-2 cells were selected. The isolates were evaluated by reduction of cholesterol absorption through an in vitro model. The expressions of several candidate genes that associated with cholesterol metabolism in Caco-2 cells were further quantified by real-time PCR. The results showed that Lactococcus lactis subsp. lactis YPK20 and YPK23 could reduce cholesterol absorption by Caco-2 cells. Not by BSH activity or assimilation of cholesterol by bacteria, pre-treatment with Lc. lactis subsp. lactis YPK20 and YPK23 could down-regulate NPC1L1 gene expression in Caco-2 cells, and down-regulation of HMGCR gene in Caco-2 cells was also observed by pre-treatment with Lc. lactis subsp. lactis YPK23. Furthermore, these two isolates showed anti-pathogenic activities by metabolites and acid tolerance at pH3. In conclusion, this study constructed a model for detecting cholesterol absorption by Caco-2 cells, and found that Lc. lactis subsp. lactis YPK20 and YPK23 possess potential abilities to regulate gene expressions and lower cholesterol absorption in Caco-2 cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T16:46:14Z (GMT). No. of bitstreams: 1 U0001-0508202014003400.pdf: 3380183 bytes, checksum: f4c797790b6ac8807ab1b6198a97ef72 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 中文摘要 i Abstract ii 目錄 iii 圖目錄 vii 表目錄 ix 第一章 文獻探討 1 一、高膽固醇血症 1 (一)膽固醇的代謝 1 (二)高膽固醇血症的成因及其與心血管疾病的關聯 2 (三)高膽固醇血症的現況 3 (四)高膽固醇血症的預防與治療 4 二、益生菌與克弗爾粒 5 (一)益生菌和乳酸菌 5 (二)克弗爾粒之起源及開發潛力 5 (三)乳酸菌預防高膽固醇血症之潛力 6 三、益生菌降低腸道內膽固醇吸收機制 7 (一)膽鹽水解酶的活性 7 (二)細菌表面吸附膽固醇微胞能力 8 (三)乳酸菌影響宿主腸道中膽固醇代謝相關基因表現 8 四、實驗動機與目的 11 第二章 材料與方法 12 一、實驗架構 12 二、克弗爾粒的活化與保存 13 三、克弗爾粒中乳酸菌分離、篩選、活化、繼代與保存 13 (一)乳酸菌的分離與篩選 13 (二)乳酸菌的活化、繼代與保存 13 四、乳酸菌菌種鑑定分析 14 (一)基本特性分析 14 (二)16S rRNA 基因定序 14 (三)rpoA基因定序 14 (四)親緣關係樹的建立 15 五、膽固醇微胞製備 15 六、小腸上皮細胞膽固醇吸收模型建立 16 (一)細胞株的活化、繼代與保存 16 (二)Caco-2細胞對膽固醇濃度毒性測試 17 (三)對正控制組之Caco-2細胞毒性測試 17 (四)對候選乳酸菌株之Caco-2細胞毒性測試 18 七、細胞吸收膽固醇之評估 19 (一)預處理模式 19 (二)膽固醇定量分析 19 (三)乳酸菌吸附膽固醇微胞能力評估 19 八、細胞膽固醇代謝基因表現量分析 20 (一)與細胞共培養之樣品製備 20 (二)樣品細胞共培養與抽取細胞RNA 21 (三)純化RNA 21 (四)cDNA的反轉錄 21 (五)即時定量聚合酶連鎖反應 22 九、菌株的生長與生化特性評估 22 (一)菌株的碳源利用能力分析與菌種確認 22 (二)耐酸試驗 22 (三)耐膽鹽試驗 23 (四)抗生素敏感性試驗 23 (五)抗病原菌能力評估 23 (六)菌株腸道貼附能力評估 23 (七)膽鹽水解酶活性定性分析 24 十、統計分析方法 25 第三章 實驗結果 34 一、乳酸菌菌種鑑定 34 (一)16S rRNA基因定序 34 (二)rpoA基因定序 34 二、小腸上皮細胞吸收模型建立 34 (一)Caco-2細胞對膽固醇濃度毒性測試 34 (二)Caco-2細胞對正控制組毒性測試 35 (三)Caco-2細胞對候選乳酸菌株的毒性測試 35 (四)微胞穩定性與染色觀察 35 三、細胞膽固醇吸收能力評估 35 四、乳酸菌吸附膽固醇微胞能力評估 36 五、細胞膽固醇代謝相關基因表現量分析 37 (一)預處理模式對細胞基因表現影響 37 (二)死菌、細胞壁、胞外液、胞內液預處理模式對細胞基因影響 37 六、乳酸菌的生長與生化特性能力評估 37 (一)潛力菌株之基本特性 37 (二)親緣關係樹建立 38 (三)碳源利用能力分析 38 (四)耐酸試驗 38 (五)耐膽鹽試驗 39 (六)抗生素敏感性測試 39 (七)病原菌抑制能力評估 39 (八)腸道貼附能力評估 39 (九)膽鹽水解酶活性定性分析 40 第四章 討論 62 一、乳酸菌鑑定 62 二、小腸上皮細胞吸收模型建立 62 三、細胞膽固醇吸收能力評估 64 四、乳酸菌吸附膽固醇能力評估 64 五、細胞膽固醇代謝相關基因表現量分析 65 六、乳酸菌的生長與生化特性能力評估 66 第五章、結論 69 第六章、參考資料 70 | |
dc.language.iso | zh-TW | |
dc.title | 以Caco-2細胞模式篩選具有減少吸收膽固醇能力之乳酸菌株 | zh_TW |
dc.title | Screening of potential lactic acid bacteria strains with the ability to reduce the absorption of cholesterol by Caco-2 cell model | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張惠雯(Hui-Wen Chang),劉啟德(Chi-Te Liu),彭及忠(Chi-Chung Peng),謝建元(Chien-Yan Hsieh) | |
dc.subject.keyword | 克弗爾粒,乳酸菌,膽固醇吸收,NPC1L1,Lactococcus lactis subsp. lactis, | zh_TW |
dc.subject.keyword | kefir grains,lactic acid bacteria,cholesterol absorption,NPC1L1,Lactococcus lactis subsp. lactis, | en |
dc.relation.page | 76 | |
dc.identifier.doi | 10.6342/NTU202002460 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-17 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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U0001-0508202014003400.pdf 目前未授權公開取用 | 3.3 MB | Adobe PDF |
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