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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳媺玫(Meei-Mei Chen) | |
| dc.contributor.author | Ting-Chieh Wang | en |
| dc.contributor.author | 汪鼎傑 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:24:59Z | - |
| dc.date.available | 2025-08-14 | |
| dc.date.copyright | 2015-08-16 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-14 | |
| dc.identifier.citation | Aiba, A., & Mizobuchi, K. (1989). Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli. Journal of Biological Chemistry, 264(35), 21239-21246.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52727 | - |
| dc.description.abstract | Lactococcus garvieae is an aquatic pathogen that causes disease in a wide range of aquatic species all over the world. It is generally believed that latent bacterial infections and resulting antibiotic resistance are caused by the in vivo formation of biofilms. Moreover, previous studies have revealed that biofilm formation causes a change in protein expression and the antigen profile available for host-recognition. In view of this, proteomic and immunoproteomic analysis were used to compare the proteomes of L. garvieae grown either as a biofilm or in the planktonic state. Proteomic analysis using two-dimensional gel electrophoresis (2-DE) revealed varying amounts of protein in nine protein spots, among which seven were up-regulated and two were down-regulated in the biofilm form when compared to the planktonic controls. Among the up-regulated proteins, four of them (glmU, gtaB, rmlB, and rfbC) participated in extracellular polymeric substances (EPS) synthesis, which is the main component of biofilm. In the immunoproteomic analysis, polyclonal rabbit antibodies raised against biofilm and planktonic bacteria reduced biofilm formation by L. garvieae, while the anti-biofilm serum showed stronger inhibition of biofilm formation. The sera were later used to perform 2-DE Western blot assays on whole cell protein extracted from both biofilm and planktonic forms. Two immunoreactive proteins specifically recognized by anti-biofilm sera were selected, among which a moonlighting protein, arginine deiminase (AD), was considered to be associated with biofilm formation, and thus may be a potential vaccine candidate against biofilm infections. | en |
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| dc.description.tableofcontents | 摘要…………………………………………………………………………………… i
Abstract………………………………………………………………………………...ii 目錄……………………………………………………………………………………iii 圖次……………………………………………………………………………...……vii 表次……………………………………………………………………………...…...viii 第一章 緒論……………………………………………………………………………1 第二章 文獻回顧 2.1格氏乳酸球菌…………………………………………………………………2 2.1.1背景和生化特性………………………………………………………...2 2.1.2流行病學和宿主………………………………………………………...2 2.2生物膜介紹……………………………………………………………………4 2.2.1生物膜簡介……………………………………………………………...4 2.2.2生物膜的生長與組成…………………………………………………...5 2.2.3生物膜內微生物的性狀及基因表現…………………………………...7 2.2.4生物膜與宿主免疫……………………………………………………...9 2.2.5生物膜的控制……………………………………………………….......11 2.3蛋白質體學……………………………………………………………………13 2.3.1蛋白質體學簡介………………………………………………………...13 2.3.2蛋白質體學與基因表現………………………………………………...14 2.3.3二維電泳簡介…………………………………………………………...15 第三章 材料與方法 3.1實驗設計及流程………………………………………………………………18 3.2格氏乳酸球菌之生物膜及懸浮菌粒培養……………………………………19 3.2.1生物膜培養……………………………………………………………...19 3.2.2懸浮菌粒培養…………………………………………………………...20 3.3細菌全蛋白質樣本製備………………………………………………….…...21 3.3.1蛋白質粗萃取…………………………………………………………...21 3.3.2蛋白質純化……………………………………………………………...22 3.4比較生物膜及懸浮菌粒之蛋白質表現……………………………………....23 3.4.1蛋白質樣本定量………………………………………………………...23 3.4.2二維電泳–等電膠集電泳……………………………………………...25 3.4.3二維電泳–聚丙烯醯胺膠片電泳……………………………………...27 3.4.4二維電泳之影像軟體分析……………………………………………...30 3.4.4.1圖檔分析前處理……………………………………………......30 3.4.4.2分析參數設定………………………………………………......31 3.5抗生物膜及抗懸浮菌粒之血清抑制試驗…………………………………....32 3.5.1生長抑制試驗………………………………………………...……........32 3.5.2生物膜抑制試驗……………………………………………...……........33 3.6比較生物膜及懸浮菌粒之抗原性與免疫原性…………………...…….........34 3.6.1二維電泳–蛋白質轉印法…………………………………...……........34 3.6.2二維電泳–西方墨點法……………………………………...……........36 3.7蛋白質身分鑑定………………………………………………………….......37 3.7.1膠體內水解………………………………………………………….......37 3.8重組表現與生物膜形成相關之抗原蛋白………………………………........40 3.8.1目標基因序列的增幅及純化……………………………………….......41 3.8.2建構含有目標基因序列的表現質體……………………………….......44 3.8.3表現重組蛋白……………………………………………………….......47 3.8.3.1表現條件測試…………………………………………...….......48 3.8.3.2大量表現重組蛋白……………………………………...….......49 3.8.4純化重組蛋白……………………………………………………….......50 3.8.5重組蛋白抗原特性檢驗…………………………………………….......52 3.9抗重組蛋白之多株抗體製備及血清抑菌能力試驗……………………........52 第四章 結果 4.1格氏乳酸球菌之生物膜及懸浮菌粒培養………………………………........54 4.2細菌全蛋白質樣本製備……………………………………………................54 4.2.1蛋白質粗萃取…………………………………………….......................54 4.2.2蛋白質純化……………………………………………...........................54 4.3比較生物膜及懸浮菌粒之蛋白質表現……………………............................55 4.3.1蛋白質樣本定量………………………………………...........................55 4.3.2二維電泳……………………………………………...............................55 4.3.3影像軟體分析………………………………………...............................55 4.4抗生物膜及抗懸浮菌粒之血清抑菌試驗……………………………............56 4.4.1生長抑制試驗………………………………………...............................56 4.4.2生物膜抑制試驗…………………………………..….............................56 4.5比較生物膜及懸浮菌粒之抗原性與免疫原性……………............................57 4.6蛋白質身份鑑定…………………………………………................................57 4.6.1生物膜和懸浮菌粒之間表現量具有差異的蛋白質...............................58 4.6.2生物膜形成相關之免疫原性蛋白…………………...............................58 4.7重組表現與生物膜形成相關之抗原蛋白………………................................59 4.7.1目標基因序列的增幅及純化………………………...............................59 4.7.2建構含有目標基因序列的表現質體………………...............................59 4.7.3表現重組蛋白………………………………………...............................59 4.7.4純化重組蛋白………………………………………...............................60 4.7.5重組蛋白之抗原特性檢驗及多珠抗體製備………………...................60 4.7.6抗重組蛋白之血清抑菌能力測試……………………………………...60 第五章 討論 5.1細菌全蛋白質樣本製備…………………………………................................61 5.2二維電泳………………………………………................................................62 5.3比較生物膜及懸浮菌粒之蛋白質表現………................................................63 5.4抗生物膜及抗懸浮菌粒之血清抑菌試驗………………………....................67 5.5比較生物膜及懸浮菌粒之抗原性與免疫原性................................................68 5.6重組表現與生物膜形成相關之抗原蛋白及多珠抗體製備............................71 5.7抗重組蛋白之血清抑菌能力測試………………............................................71 第六章 參考文獻………………………………………...............................................87 | |
| dc.language.iso | zh-TW | |
| dc.title | 格氏乳酸球菌在懸浮態和生物膜態之蛋白質表現分析及辨識生物膜形成相關的免疫原性蛋白 | zh_TW |
| dc.title | Proteomic Analysis of Lactococcus garvieae Cultivated under Planktonic and Biofilm Conditions and Identification of Biofilm Formation-Related Immunogenic Proteins | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張本恆,張錦宜,黃子鳴 | |
| dc.subject.keyword | 格氏乳酸球菌,生物膜,二維電泳,免疫蛋白質體學,精氨酸脫亞氨?, | zh_TW |
| dc.subject.keyword | Lactococcus garvieae,biofilm,two-dimensional gel electrophoresis,immunoproteomic,arginine deiminase, | en |
| dc.relation.page | 100 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-14 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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