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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 繆希椿(Shi-Chuen Miaw) | |
| dc.contributor.author | Wei-Ting Wang | en |
| dc.contributor.author | 王韋婷 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:19:48Z | - |
| dc.date.available | 2020-09-24 | |
| dc.date.copyright | 2015-09-24 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-16 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52595 | - |
| dc.description.abstract | 半乳糖凝集素-3 (Galectin-3)是半乳糖凝集素家族的一員，含有能特定和半乳糖苷結合的編碼區 (CRD)。Galectin-3的功能十分多樣化，包含活化、黏附、細胞凋亡、細胞遷移和吞噬作用。 不變的自然殺手T細胞 (iNKT cell)的T細胞受體 (TCRs)會專一性地去辨認CD1d-α-GalCer複合體。經由CD1d-α-GalCer的刺激後，iNKT cell 會快速且大量地產生細胞激素 (cytokines)。這些由iNKT cell所產生的cytokines，經報導證實在許多過敏性、自體免疫及感染性疾病的致病過程中扮演重要的角色。雖然目前已知Galectin-3參與在人類疾病和免疫反應，但是Galectin-3在iNKT cell產生cytokines及細胞相關的發炎反應的影響尚未釐清。 在我們的研究中，我們首先檢驗Galectin-3是否有表現在iNKT cell中。我們發現Galectin-3在胰臟、腸繫膜淋巴結及周邊淋巴結的iNKT cell中皆有表現。 接下來我們使用野生型(WT)及半乳糖凝集素-3缺失型(Gal-3 KO)的小鼠，觀察在注射α-GalCer後iNKT cell的族群數量。我們得知Galectin-3的缺失並不會影響iNKT cell在胰臟、腸繫膜淋巴結及周邊淋巴結的族群數目。我們更進一步研究以體內施打α-GalCer以刺激小鼠，觀察其iNKT cell的細胞激素分佈型 (cytokine profile)。我們的結果顯示Galectin-3缺失的周邊淋巴結iNKT cell跟野生型比較顯著地分泌較少的介白素-17 (IL-17)。 研究結果指出Galectin-3在iNKT cell所調節的IL-17分泌中，扮演了正向的特定性角色。 | zh_TW |
| dc.description.abstract | Galectin-3 (gal-3), a member of the galectin family, contains CRD that enable the specific binding of β-galactosides. The functions of galectin-3 are diverse, including activation, adhesion, apoptosis, cell migration and phagocytosis. The T cell receptors (TCRs) of invariant NKT (iNKT) cells specifically recognize CD1d-α-GalCer complex. Upon activation by CD1d-α-GalCer, iNKT cells rapidly produce large amounts of cytokines. These cytokines produced by iNKT cells have been reported to play a crucial role in the pathogenesis of various allergic, autoimmune and infectious diseases. Although galectin-3 is involved in various human diseases and immune responses, its role in cytokine production by iNKT cells and the effect in iNKT cell-mediated inflammation remain unclear. In our study, we first examined whether galectin-3 is expressed by iNKT cells. We found that galectin-3 is expressed in iNKT cells from spleen (SP), mesenteric lymph nodes (mLNs) and peripheral lymph nodes (pLNs). We next used WT and Gal-3 KO mice to examine the population of iNKT cells after injection with α-GalCer. Deficiency of galectin-3 doesn’t affect the iNKT population in SP, mLN and pLN. We further studied the cytokine profile of iNKT cells after stimulation with α-GalCer in vivo. Our data indicated the galectin-3-deficent iNKT cells in pLN secret less IL-17 than WT iNKT cells. Our results suggest that the positive and specific role of galectine-3 in regulating IL-17 production by iNKT cells. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:19:48Z (GMT). No. of bitstreams: 1 ntu-104-R02449003-1.pdf: 2177994 bytes, checksum: 48b1890dfa73880a70b5b7c1427deef5 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | Acknowledgements i 中文摘要 ii Abstract iii Table of contents iv Introduction 1 1. Galectin-3 1 2. iNKT cells and its function in immune system 2 3. Overview of IL-17 4 4. Significance and specific aim 6 Materials and Methods 7 1. Materials 7 1.1 Chemicals and Reagents 7 1.2 Antibodies 8 1.3 Cytokines 9 1.4 Kits 9 1.5 Mice 9 2. Methods 11 2.1. Expansion for iNKT cells 11 2.2. Real-time PCR analysis 11 2.3. Galectin-3 expression analysis 12 2.4. In vivo stimulation and cytokine analysis 12 2.5. Polarization of iNKT 17 cells and ELISA analysis 13 Results 14 1. Galectin-3 is expressed in iNKT cells. 14 2. Population of iNKT cells in the spleen, mesenteric lymph nodes and peripheral lymph nodes from wild type and galectin-3 knockout mice is comparable. 15 3. Upon α-GalCer stimulation, iNKT cells from the peripheral lymph nodes of galectin-3 knockout mice secret less IL-17A than WT iNKT cells. 16 4. Invariant NKT17-skewed cells from spleen of galectin-3 knockout mice secret less IL-17 than iNKT17-skewed cells from spleen of WT mice. 18 5. The neutrophil recruitment in the airway of WT and Gal-3 KO mice is comparable after 48 hours stimulation with α-GalCer intratracheally. 18 Discussion 20 Figures 22 Figure 1. Differential expression of galectin family in iNKT cells. 24 Figure 2. Expression of galectin-3 in iNKT cells. 26 Figure 3. Population of iNKT cells in spleen, mesenteric lymph nodes and peripheral lymph nodes from wild type and galectin-knockout mice is comparable. 28 Figure 4. Cytokines expression of iNKT cells in spleen, mesenteric lymph nodes and peripheral lymph nodes of wild type and galectin-3 knockout mice. 30 Figure 5. The IL-17 production by iNKT17-skewed cells from spleen of wild type and galectin-3 knockout mice was determined by ELISA. 32 Figure 6. Neutrophil recruitment in the airway of wild type and galectin-3 knockout mice is comparable after 48 hours stimulation with α-GalCer intratracheally. 34 References 35 | |
| dc.language.iso | en | |
| dc.subject | 自然殺手T細胞 | zh_TW |
| dc.subject | 介白素-17 | zh_TW |
| dc.subject | 半乳糖凝集素-3 | zh_TW |
| dc.subject | iNKT | en |
| dc.subject | galectin-3 | en |
| dc.subject | IL-17 | en |
| dc.title | 半乳糖凝集素-3在iNKT細胞產生的介白素-17中所扮演的角色 | zh_TW |
| dc.title | The role of galectin-3 in IL-17 production by iNKT cell | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 伍安怡(Betty Wu-Hsieh),劉扶東(Fu-Tong Liu),張雅貞(Ya-Jen Chang) | |
| dc.subject.keyword | 半乳糖凝集素-3,自然殺手T細胞,介白素-17, | zh_TW |
| dc.subject.keyword | galectin-3,IL-17,iNKT, | en |
| dc.relation.page | 43 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-17 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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