以嵌入式系統作為自動化可攜帶式表面電漿共振(SPR)感測器平台

Abstract

中文摘要 隨著醫療技術不斷的發展，生物感測器的出現逐漸滿足了各種生物分子檢測的需求。另外，生物感測器也提供醫師或醫檢師量化的數據以方便診斷各種疾病，因此奠定了檢測醫學的里程碑。但由於醫療場所上所使用的生物感測器，居多為造價昂貴、不易攜帶的感測器，非一般使用者能使用，且須送到中央實驗室進行檢測，因此相當的花時間且很沒有效率。為了要改善這些問題，如何發展出一種具有經濟效益(cost-effective)、可攜帶的且一般人均可在任一定點使用的感測器是本論文的著重的方向。 本論文使用的感測器為表面電漿共振(Surface Plasmon Resonance , SPR)感測器，是一種可以即時監測且具高靈敏度(sensitivity)的感測器。由於不需要標定的(Label-free)1的緣故，製程相較於其他光學感測器省時以及便利，因此常被拿來使用。 為了讓使用者方便攜帶和操作，在此用嵌入式系統Raspberry Pi (RPi) 做為感測器的平台。其中有圖形化介面(Graphical User Interface , GUI)輔助使用者進行操作，包括測量、校正和分析三個部分。 目前經過酒精校正之後，確定系統的靈敏度可以達到2.8x10-4reflective index per unit (RIU)，之後會用利用此平台量測並分析生物分子檢測以達到目的：可攜帶式SPR生物感測器。

Abstract With the improvement of medical techniques, biosensor has developed for the need of bio-molecules testing, which provides quantified data to doctors for diagnosis and becomes the milestone of clinical laboratory testing. However, most of them are expansive and non-portable devices, which have to be analyzed through special users, medical laboratory scientist (MLS), in the center and make them time-consuming devices. So the purpose of the thesis is to develop a cost-effective and portable device, which can be used for anyone. In the thesis, we use Surface Plasmon Resonance (SPR) sensor as our sensor, which can do real-time analysis and provide high sensitivity. Compare to other optical devices, the procedure of SPR testing, a kind of label-free testing, is much easier. These are the reasons why SPR sensors have been used so frequently. For developing a potable sensor with easy user interface, we use embedded system Raspberry Pi (RPi) as our sensor’s platform. In the software of RPi, Graphical User Interface (GUI) is presented for guiding end-users to manipulate the SPR system, including three main workflows: Testing, Calibration, and Analysis. Recently, we have done the ethanol calibration, and the sensitivity is 2.8x10-4reflective index unit (RIU). After that, we will manipulate the platform to do the bio-molecule testing for developing portable SPR biosensor.