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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾琬瑜(Wan-Yu Tseng) | |
dc.contributor.author | Wei-Chun Lin | en |
dc.contributor.author | 林煒峻 | zh_TW |
dc.date.accessioned | 2021-06-15T16:12:12Z | - |
dc.date.available | 2015-09-24 | |
dc.date.copyright | 2015-09-24 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-18 | |
dc.identifier.citation | 1. Misch, C.E., Contemporary implant dentistry. 3 ed. 2008: Mosby Elsevier.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52337 | - |
dc.description.abstract | 牙科植體(dental implant)於近年來蓬勃發展,是當前受矚目的牙科治療技術。透過人工牙根與患者齒槽骨達成骨整合(osseointegration),使病人在無牙區的贗復上能有更能承受咬合力量的治療選擇,在過往的研究中顯示牙科植體的表面處理方式對於骨整合的成功與否影響深遠。
當前最廣泛使用的人工牙根材料以商業級純鈦(commercially pure titanium, CPTi)及鈦六鋁四釩(Ti6Al4V)等鈦金屬為主。此類鈦金屬在自然環境下會在極短時間內形成二氧化鈦保護層(passive layer),然而此二氧化鈦保護層為非晶形(amorphous)結構,且厚度只有約5–6奈米。為了產生緻密度更佳、厚度更厚的二氧化鈦保護層以提供更好的成骨細胞(osteoblastic cells)生長環境,將使用原子層沉積技術(atomic layer deposition, ALD)於純鈦及鈦六鋁四釩表面成長二氧化鈦鍍膜,測試厚度包含0奈米,20奈米,50奈米,100奈米;測試晶形包含非晶形與銳鈦礦形(anatase)二氧化鈦。我們預期經由原子層沉積技術成長二氧化鈦保護層的鈦金屬能提高其生物相容性(biocompatibility)。 本研究主要分為材料分析及細胞實驗兩個主要部分。材料分析方面包含電子顯微鏡(scanning electron microscope, SEM)以及原子力顯微鏡(atomic force microscope, AFM)。細胞實驗部分包含細胞存活率(cell viability)、鹼性磷酸酶試驗(alkaline phosphatase assay, ALP assay)、骨鈣素試驗(osteocalcin assay, OCN assay)以及免疫螢光染色試驗(Immunofluorescence assay, IFA)。 | zh_TW |
dc.description.abstract | Dental implant has been very popular nowadays, and implant therapy has also been in the limelight. By osseointegrated with patient’s alveolar bone, dental implants provide dentists another prosthetic treatment choice which can bear more occlusal force during chewing. Previous research pointed out that implant surface treatment may play an important role on implant osseointegration.
The most commonly used dental implant materials are commercially pure titanium and Ti6Al4V. In natural environment, these titanium alloy mantles a cover of titanium dioxide passive layer in short time. Nevertheless, the cover of titanium dioxide is amorphous type and its depth is about 5-6 nm. For producing a more intensive and thicker cover of titanium dioxide to provide better environment for osteoblastic cells, atomic layer deposition technique is used. In this research, we tested four different thickness(0 nm, 20 nm, 50 nm, 100 nm)and two different crystal forms(amorphous, anatase)of titanium dioxide. Thicker titanium dioxides passive layer as expected to be more biocompatible. This research has two main parts, material analyses and cell experiments. Atomic force microscope, scanning electron microscope were carried out in material analyses. Cell viability, alkaline phosphatase assay, osteocalcin assay and immunofluorescence assay were carried out in cell experiments. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T16:12:12Z (GMT). No. of bitstreams: 1 ntu-104-R01422003-1.pdf: 19492732 bytes, checksum: cfb4878dda08a35017b167ca72e76471 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 iii Abstract iv 目錄 v 圖目錄 ix 表目錄 xvi 第一章 緒論 1 1.1 牙科植體之現況 1 1.2 原子層沉積技術 5 第二章 研究動機與目的 9 2.1 研究動機 9 2.2 研究目的 10 第三章 文獻回顧 11 3.1 鈦金屬 11 3.1.1 商業級純鈦與鈦六鋁四釩 14 3.1.2 二氧化鈦保護層 16 3.2 骨整合 17 3.3牙科植體表面處理 19 3.3.1 機械加工植體表面(Machined surface) 21 3.3.2 鈦電漿噴覆植體表面(Titanium plasma sprayed surface) 22 3.3.3 噴砂處理植體表面(Sandblasted surface) 22 3.3.4 酸蝕處理植體表面(Acid etched surface) 24 3.3.5 噴砂及酸蝕植體表面(Sandblasted and acid etched surface, SLA surface) 25 3.3.6 雷射處理植體表面(Laser treated surface) 27 3.3.7 陽極氧化處理植體表面(Anodized implant surface) 28 3.3.8 生物活性物質披覆植體表面(Bioactive material coated surface) 29 3.3.9 總結 30 第四章 實驗材料與方法 32 4.1 實驗流程圖 32 4.2 實驗材料製備 33 4.3 實驗細胞及培養環境 34 4.4 掃描式電子顯微鏡(SEM) 35 4.4.1 實驗步驟 35 4.5 Alamar blue assay 36 4.5.1 說明 36 4.5.2 實驗步驟 38 4.6 MTT assay 39 4.6.1 說明 39 4.6.2 實驗步驟 39 4.7 鹼性磷酸酶試驗(ALP assay) 40 4.7.1 說明 40 4.7.2 實驗步驟 41 4.8 骨鈣素試驗(Osteocalcin assay) 43 4.8.1 說明 43 4.8.2 實驗步驟 44 4.9 免疫螢光染色試驗(Immunofluorescence assay, IFA) 45 4.9.1 說明 45 4.9.2 實驗步驟 45 4.10 原子力顯微鏡(Atomic force microscope) 47 4.10.1 說明 47 4.10.2 實驗步驟 47 4.11 統計分析 48 第五章 實驗結果 50 5.1 掃描式電子顯微鏡(SEM) 50 5.2 Alamar blue assay 51 5.3 MTT assay 58 5.4 鹼性磷酸酶試驗(ALP assay) 60 5.5 骨鈣素試驗(Osteocalcin assay) 66 5.6 免疫螢光染色試驗(Immunofluorescence assay, IFA) 72 5.7 原子力顯微鏡(Atomic force microscope) 73 第六章 討論 78 6.1 材料測試 78 6.1.1 非晶形二氧化鈦鍍膜 78 6.1.2 銳鈦礦形二氧化鈦鍍膜 81 6.2 細胞實驗 83 6.2.1 生物相容性 84 6.2.2 體外細胞礦化能力分析 87 6.2.3 細胞型態 90 6.3 未來研究方向 95 第七章 結論 96 參考文獻 97 圖附錄 105 | |
dc.language.iso | zh-TW | |
dc.title | 利用原子層沉積技術改質牙科植體表面及其表現之研究 | zh_TW |
dc.title | Research on the Dental Implant Surface Properties and Performances Using Atomic Layer Deposition Technique | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林立德(Li-De Lin),洪志遠(Zhi-Yuan Hong) | |
dc.subject.keyword | 植體表面處理,原子層沉積技術,二氧化鈦, | zh_TW |
dc.subject.keyword | implant surface treatment,atomic layer deposition techinique,titanium dioxide, | en |
dc.relation.page | 138 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-18 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 19.04 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。