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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51984完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達 | |
| dc.contributor.author | Kai-Chun Lin | en |
| dc.contributor.author | 林愷浚 | zh_TW |
| dc.date.accessioned | 2021-06-15T14:01:14Z | - |
| dc.date.available | 2020-08-28 | |
| dc.date.copyright | 2015-08-28 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-20 | |
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Yoon H, Han N, Kim C (2004) Expression of Thermotoga maritima endo-β-1, 4-xylanase gene in E. coli and characterization of the recombinant enzyme. Agricultural Chemistry and Biotechnology. 林軒立，2010。納豆菌漆酶之異源表現。國立臺灣大學生命科學院微生物與生化學研究所碩士論文。 張惠芬，2014。由Paenibacillus macerans生產耐熱型木聚醣酶。國立臺灣大學生命科學院生化科技學系碩士班論文。 石馥維，2005。血纖維分解酶subtilisin NAT之發酵生產研究。國立臺灣大學生命科學院微生物與生化學研究所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51984 | - |
| dc.description.abstract | 由本實驗室所篩選出具有產生最高活性之木聚醣酶之Bacillus subtilis natto #14，其發酵上清液酵素最適活性條件為pH 5、50℃。利用合成培養基之銨鹽化合物及少量酵母萃取物作為氮源，並比較葡萄糖、蔗糖、木糖、甘油對其生長之影響，以葡萄糖作為最適培養碳源。使用多種誘導木聚醣酶之誘導取代物，如山毛櫸木聚醣、小麥麩皮、黃豆粉、白楊木，發現10%小麥麩皮所誘導之木聚醣酶活性比使用山毛櫸木聚醣誘導之對照組更高。測試葡萄糖對誘導木聚醣酶之影響，發現葡萄糖並不抑制木聚醣酶生成，且可誘導更多木聚醣酶活性產生。以合成培養基於搖瓶培養後第12小時可獲得12.1 U/mL的木聚醣酶活性，而於發酵槽饋料培養後在第12個小時可獲得最高木聚醣酶活性59.2 U/mL。 | zh_TW |
| dc.description.abstract | Bacillus subtilis natto #14 has the highest xylanase activity which optimum temperature and pH is 50℃ and pH 5. We used synthetic medium with ammonium salts and little yeast extract as nitrogen source. Compared with glucose, sucrose, xylose, and glycerol, the most suitable carbon source in growth stage was glucose. Compared with beechwood xylan, wheat bran, soya powder, and white poplar powder, the best inducer for xylanase production was wheat bran. Induction of xylanase activity by 10% wheat bran was higher than induction by beechwood xylan. While grown with glucose and xylan, we found that glucose would help to induce xylanase activity, instead of inhibition. While grown in synthetic medium by flask culture, we could obtain the xylanase activity with 12.1 U/mL for 12 hours. While grown in bioreactor, we could obtain the highest xylanase activity with 59.2 U/mL for 12 hours. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T14:01:14Z (GMT). No. of bitstreams: 1 ntu-104-R02b22027-1.pdf: 1696132 bytes, checksum: 44af9fdec10ba90886ca668af237679e (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 中文摘要 II Abstract III 縮寫表 IV 中英文對照表 V 目錄 VI 圖表目錄 IX 第一章 前言 1 1.1 納豆菌 (Bacillus subtilis natto) 2 1.2 木聚醣 3 1.2.1 木聚醣 3 1.2.2 木聚醣降解系統 4 1.3 內切型木聚醣酶 (β-1,4-endoxylanase) 4 1.3.1 內切型木聚醣酶之分類 4 1.3.2 內切型木聚醣酶之特性 5 1.3.3 內切型木聚醣酶之誘導及調控 6 1.3.4 內切型木聚醣酶之應用 7 1.3.5 內切型木聚醣酶之來源與生產 9 1.4 研究動機與目的 10 1.5 研究大綱 11 第二章 材料與方法 13 2.1 菌株來源 14 2.2 納豆菌之篩選 14 2.3 木聚醣酶活性測定 14 2.4 納豆菌之搖瓶培養 15 2.5 生產木聚醣酶之誘導物篩選 15 2.6 碳源抑制木聚醣酶生產之測試 16 2.7 納豆菌之發酵槽培養 16 2.8 培養液中殘糖測定 17 第三章 結果 19 3.1 納豆菌篩選結果 20 3.2 木聚醣酶活性測試之反應條件最適化 20 3.3 納豆菌之搖瓶培養最適化 21 3.3.1 培養溫度 21 3.3.2 培養基碳源最適化 21 3.3.3 誘導物之篩選 21 3.3.4 木聚醣酶生產之誘導物條件最適化 22 3.4 葡萄糖對木聚醣酶生產之影響 22 3.5 納豆菌之發酵槽培養 23 第四章 討論與結論 27 4.1 討論 28 4.1.1 發酵槽生產木聚醣酶 28 4.1.2 不同發酵技術生產木聚醣酶 28 4.1.3 未來展望 29 4.2 結論 30 圖表 31 參考文獻 47 附錄 55 | |
| dc.language.iso | zh-TW | |
| dc.subject | 納豆菌 | zh_TW |
| dc.subject | 發酵槽 | zh_TW |
| dc.subject | 小麥麩皮 | zh_TW |
| dc.subject | 木聚醣 | zh_TW |
| dc.subject | 木聚醣? | zh_TW |
| dc.subject | bioreactor | en |
| dc.subject | wheat bran | en |
| dc.subject | xylan | en |
| dc.subject | Bacillus subtilis natto | en |
| dc.subject | xylanase | en |
| dc.title | 以納豆菌生產木聚醣酶 | zh_TW |
| dc.title | Production of xylanase using Bacillus subtilis natto | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃鵬林,楊健志,劉?德 | |
| dc.subject.keyword | 納豆菌,木聚醣?,木聚醣,小麥麩皮,發酵槽, | zh_TW |
| dc.subject.keyword | Bacillus subtilis natto,xylanase,xylan,wheat bran,bioreactor, | en |
| dc.relation.page | 58 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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