請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51945
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈偉強(Wei-Chiang Shen) | |
dc.contributor.author | Syun-Wun Liang | en |
dc.contributor.author | 梁薰文 | zh_TW |
dc.date.accessioned | 2021-06-15T13:58:51Z | - |
dc.date.available | 2025-08-20 | |
dc.date.copyright | 2015-08-25 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-21 | |
dc.identifier.citation | Albert, H.H., Schenck, S., 1996. PCR amplification from a homolog of the bE mating-type gene as a sensitive assay for the presence of Ustilago scitaminea DNA. Plant Dis. 80, 1189-1192. Bakkeren, G., Gibbard, B., Yee, A., Froeliger, E., Leong, S., Kronstad, J., 1992. The a and b loci of Ustilago maydis hybridize with DNA sequences from other smut fungi. Mol. Plant Microbe Interact. 5, 347-355. Bakkeren, G., Jiang, G., Warren, R. L., Butterfield, Y., Shin, H., Chiu, R., Linning, R., Schein, J., Lee, N., Hu, G., Kupfer, D.M., Tang, Y., Roe, B.A., Jones, S., Marra, M., Kronstad, J.W., 2006. Mating factor linkage and genome evolution in basidiomycetous pathogens of cereals. Fungal Genet. Biol. 43, 655-666. Bakkeren, G., Kronstad, J.W., 1994. Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi. Proc. Natl. Acad. Sci. USA. 91, 7085-7089. Bauer, R., Begerow, D., Oberwinkler, E., Piepenbring, M., Berbee, M.L., 2001. Ustilaginomycetes, in: McLaughlin, D.J., McLaughlin, E.G., Lemke, P.A. (Eds.), The Mycota VII Part B-Systematics and Evolution. Springer-Verlag, Berlin Heidelberg, Germany, pp. 57-83. Bӧlker, M., Urban, M., Kahmann, R., 1992. The a mating type locus of U. maydis specifies cell signaling components. Cell 68, 441-450. Brefort, T., Doehlemann, G., Mendoza-Mendoza, A., Reissmann, S., Djamei, A., Kahmann, R., 2009. Ustilago maydis as a Pathogen. Annu. Rev. Phytopathol. 47, 423-445. Chan, Y.S., Thrower, L.B., 1980. The host-parasite relationship between Zizania caduciflora Turcz and Ustilago esculenta P. Henn. IV. Growth substances in the host-parasite combination. New Phytol. 85, 225-233. Chiu, S.W., Moore, D., 1999. Sexual development of higher fungi, in: Oliver, R.P., Schweizer, M. (Eds.), Molecular Fungal Biology. Cambridge University Press, Cambridge, pp. 231-271. Chung, K.R., Tzeng, D.D., 2004. Biosynthesis of indole-3-acetic acid by the gall-inducing fungus Ustilago esculenta. J. Biol. Sci. 4, 744-750. Daboussi, M.J., 1997. Fungal transposable elements and genome evolution. Genetica 100, 253-260. Daboussi, M.J., Langin, J., Brygoo, Y., 1992. Fotl, a new family of fungal transposable elements. Mol. Gen. Genet. 232, 12-16. Doehlemann, G., Schirawski, J., Kamper, J., 2014. Functional genomics of smut fungi: from genome sequencing to protein function. Adv. Bot. Res. 70, 143-172. Fedler, M., Luh, K.S., Stelter, K., Nieto-Jacobo, F., Basse, C.W., 2009. The a2 mating-type locus genes lga2 and rga2 direct uniparental mitochondrial DNA (mtDNA) inheritance and constrain mtDNA recombination during sexual development of Ustilago maydis. Genetics 181, 847-860. Frueliger, E.H., Leung, S.A., 1991. The a mating-type alleles of Ustilago maydis are idiomorphs. Genes 100, 113-122. Grandaubert, J., Lowe, R.G., Soyer, J.L., Schoch, C.L., Van de Wouw, A.P., Fudal, I., Robbertse, B., Lapalu, N., Links, M.G., Ollivier, B., Linglin, J., Barbe, V., Mangenot, S., Cruaud, C., Borhan, H., Howlett, B.J., Balesdent, M.H., Rouxel, T., 2014. Transposable element-assisted evolution and adaptation to host plant within the Leptosphaeria maculans-Leptosphaeria biglobosa species complex of fungal pathogens. BMC Genomics 15, 891-917. Hanna, W.F., 1929. Studies in the physiology and cytology of Ustilago zeae and Sorosporium reilianum. Phytopathology 19, 415-441. Hartmann, H.A., Kahmann, R., Bolker, M., 1996. The pheromone response factor coordinates filamentous growth and pathogenicity in Ustilago maydis. EMBO J. 15, 1632-1641. Heitman, J., Kronstad, J., Taylor, J., Casselton, L., 2007. Sex in fungi: molecular determination and evolutionary implications. ASM Press, Washington DC. Hoffmann, J.A., Kendrick, E.L., 1968. Genetic control of compatibility in Tilletia controversa. Phytopathology 59, 79-83. Horns, F., Petit, E., Yockteng, R., Hood, M.E., 2012. Patterns of repeat-induced point mutation in transposable elements of basidiomycete fungi. Genome Biol. Evol. 4, 240-247. Hu, G.G., Linning, R., Bakkeren, G., 2002. Sporidial mating and infection process of the smut fungus, Ustilago hordei, in susceptible barley. Can. J. Botany 80, 1103-1114. Kamper, J., Kahmann, R., Bolker, M., Ma, L.J., Brefort, T., Saville, B.J., Banuett, F., Kronstad, J.W., Gold, S.E., Muller, O., Perlin, M.H., Wosten, H.A., de Vries, R., Ruiz-Herrera, J., Reynaga-Pena, C.G., Snetselaar, K., McCann, M., Perez-Martin, J., Feldbrugge, M., Basse, C.W., Steinberg, G., Ibeas, J. I., Holloman, W., Guzman, P., Farman, M., Stajich, J.E., Sentandreu, R., Gonzalez-Prieto, J. M., Kennell, J.C., Molina, L., Schirawski, J., Mendoza-Mendoza, A., Greilinger, D., Munch, K., Rossel, N., Scherer, M., Vranes, M., Ladendorf, O., Vincon, V., Fuchs, U., Sandrock, B., Meng, S., Ho, E.C., Cahill, M.J., Boyce, K.J., Klose, J., Klosterman, S.J., Deelstra, H.J., Ortiz-Castellanos, L., Li, W., Sanchez-Alonso, P., Schreier, P.H., Hauser-Hahn, I., Vaupel, M., Koopmann, E., Friedrich, G., Voss, H., Schluter, T., Margolis, J., Platt, D., Swimmer, C., Gnirke, A., Chen, F., Vysotskaia, V., Mannhaupt, G., Guldener, U., Munsterkotter, M., Haase, D., Oesterheld, M., Mewes, H.W., Mauceli, E.W., DeCaprio, D., Wade, C.M., Butler, J., Young, S., Jaffe, D.B., Calvo, S., Nusbaum, C., Galagan, J., Birren, B.W., 2006. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature 444, 97-101. Kellner, R., Vollmeister, E., Feldbrugge, M., Begerow, D., 2011. Interspecific sex in grass smuts and the genetic diversity of their pheromone-receptor system. PLoS Genet. 7, e1002436. Kent, W.J., Baertsch, R., Hinrichs, A., Miller, W., Haussler, D., 2003. Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc. Natl. Acad. Sci. USA. 100, 11484-11489. Kues, U., James, T.Y., Heitman, J., 2011. 6 mating type in Basidiomycetes: unipolar, bipolar, and tetrapolar patterns of sexuality, in: Poggeler, S., Wostemeyer, J. (Eds.), The Mycota XIV-Evolution of Fungi and Fungal-Like Organisms. Springer-Verlag, Berlin Heidelberg Germany, pp. 97-160. Laurie, J.D., Ali, S., Linning, R., Mannhaupt, G., Wong, P., Guldener, U., Munsterkotter, M., Moore, R., Kahmann, R., Bakkeren, G., Schirawski, J., 2012. Genome comparison of barley and maize smut fungi reveals targeted loss of RNA silencing components and species-specific presence of transposable elements. Plant Cell 24, 1733-1745. Lee, N., Bakkeren, G., Wong, K., Sherwood, J.E., Kronstad, J.W., 1999. The mating-type and pathogenicity locus of the fungus Ustilago hordei spans a 500-kb region. Proc. Natl. Acad. Sci. USA. 96, 15026-15031. Liu, K.H., Yeh, Y.L., Shen, W.C., 2011. Fast preparation of fungal DNA for PCR screening. J. Microbiol. Meth. 85, 170-172. McTaggart, A.R., Shivas, R.G., Geering, A.D., Callaghan, B., Vanky, K., Scharaschkin, T., 2012a. Soral synapomorphies are significant for the systematics of the Ustilago-Sporisorium-Macalpinomyces complex (Ustilaginaceae). Persoonia 29, 63-77. McTaggart, A.R., Shivas, R.G., Geering, A.D., Vanky, K., Scharaschkin, T., 2012b. A review of the Ustilago-Sporisorium-Macalpinomyces complex. Persoonia 29, 55-62. Piepenbring, M., Stoll, M., Oberwinkler, F., 2002. The generic position of Ustilago maydis, Ustilago scitaminea, and Ustilago esculenta (Ustilaginaceae). Mycol. Prog. 1, 71-80. Puhalla, J.E., 1968. Compatibility reactions on solid medium and interstrain inhibition in Uatilago maydis. Genetics 60, 461-474. Reineke, G., Heinze, B., Schirawski, J., Buettner, H., Kahmann, R., Basse, C.W., 2008. Indole-3-acetic acid (IAA) biosynthesis in the smut fungus Ustilago maydis and its relevance for increased IAA levels in infected tissue and host tumour formation. Mol. Plant Pathol. 9, 339-355. Scherer, M., Heimel, K., Starke, V., Kamper, J., 2006. The Clp1 protein is required for clamp formation and pathogenic development of Ustilago maydis. Plant Cell 18, 2388-2401. Schirawski, J., Heinze, B., Wagenknecht, M., Kahmann, R., 2005. Mating type loci of Sporisorium reilianum: novel pattern with three a and multiple b specificities. Eukaryot. Cell. 4, 1317-1327. Schirawski, J., Mannhaupt, G., Munch, K., Brefort, T., Schipper, K., Doehlemann, G., Di Stasio, M., Rossel, N., Mendoza-Mendoza, A., Pester, D., Muller, O., Winterberg, B., Meyer, E., Ghareeb, H., Wollenberg, T., Munsterkotter, M., Wong, P., Walter, M., Stukenbrock, E., Guldener, U., Kahmann, R., 2010. Pathogenicity determinants in smut fungi revealed by genome comparison. Science 330, 1546-1548. Schwartz, S., Kent, W.J., Smit, A., Zhang, Z., Baertsch, R., Hardison, R.C., Haussler, D., Miller, W., 2003. Human-mouse alignments with BLASTZ. Genome Res. 13, 103-107. Shimizu, K., Keller, N.P., 2001. Genetic involvement of a cAMP-dependent protein kinase in a G protein signaling pathway regulating morphological and chemical transitions in Aspergillus nidulans. Genetics 157, 591-600. Stakman, C., Christensen, J., 1927. Heterothallism in Ustilago zeae. Phytopathology 17, 827-834. Stanke, M., Diekhans, M., Baertsch, R., Haussler, D., 2008. Using native and syntenically mapped cDNA alignments to improve de novo gene finding. Bioinformatics 24, 637-644. Steinberg, G., Perez-Martin, J., 2008. Ustilago maydis, a new fungal model system for cell biology. Trends. Cell Biol. 18, 61-67. Ter-Hovhannisyan, V., Lomsadze, A., Chernoff, Y.O., Borodovsky, M., 2008. Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training. Genome Res. 18, 1979-1990. Urban, M., Kahmann, R., Bӧlker, M., 1996a. The biallelic a mating type locus of Ustilago maydis: remnants of an additional pheromone gene indicate evolution from a multiallelic ancestor. Mol. Gen. Genet. 250, 414-420. Urban, M., Kahmann, R., Bӧlker, M., 1996b. Identification of the pheromone response element in Ustilago maydis. Mol. Gen. Genet. 251, 31-37. Vanky, K., 2002. Illustrated genera of smut fungi, second ed. APS press, USA. Yang, H.C., Leu, L.S., 1978. Formation and histopathology of galls induced by Ustilago esculenta in Zizania latifolia. Phytopathology 68, 1572-1576. You, W., Liu, Q., Zou, K., Yu, X., Cui, H., Ye, Z., 2011. Morphological and molecular differences in two strains of Ustilago esculenta. Curr. Microbiol. 62, 44-54. Zhang, J.Z., Chu, F.Q., Guo, D.P., Hyde, K.D., Xie, G.L., 2011. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia. Mycol. Prog. 11, 499-508. Zhang, J.Z., Chu, F.Q., Guo, D.P., Ojaghian, M.R., Hyde, K.D., 2013. The vacuoles containing multivesicular bodies: a new observation in interaction between Ustilago esculenta and Zizania latifolia. Eur. J. Plant Pathol. 138, 79-91. 林天枝(2005)。茭白筍。臺灣農家要覽增修訂三版-農作篇(二),271-276。 林孟輝、方再秋、郭建志、姜金龍、李窓明、鄭隨和(2009)。茭白新品種「桃園1號」簡介。農政與農情,201,157-159。 洪聖峰(1997)。應用RAPD技術於茭白品種及其黑穗菌系鑑別之硏究。未出版之碩士論文,國立臺灣大學園藝學研究所,臺北市。 高紀清(1995)。應用RAPD 鑑別茭白品種之研究。未出版之碩士論文,國立臺灣大學園藝學研究所,臺北市。 黃晉興(2014a)。茭白筍之形成原因與發展史。農業試驗所技術服務季刊, 98,11-14。 黃晉興(2014b)。茭白筍之栽培與利用。農業試驗所技術服務季刊,頁7-10。 黃晉興、安寶貞(2008)。茭白筍產期調節與周年計畫生產。農業試驗所技術服務季刊,74,12-14。 楊秀珠(1976)。茭白筍之貯藏,組織解剖及其病原菌 Ustilago esculenta Henning 之研究。未出版之碩士論文,國立中興大學植物病理研究所,臺灣臺中市。 蔡正宏、陳世芳(2012)。茭白筍。臺中區農業改良場特刊,112,98-109。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51945 | - |
dc.description.abstract | 茭白(Zizania latifolia Turcz.),為禾本科多年生水生植物,受到茭白筍黑穗菌(Ustilago esculenta)侵染,造成莖基部膨大,而成為常見且供食用的茭白筍。茭白主要分布於中國、日本、臺灣等地,在臺灣以青殼及赤殼品種為主。目前,茭白與共生黑穗菌的相關研究有限,而其近緣模式物種玉米黑穗病菌(Ustilago maydis)的研究指出,其感染與有性生殖有關,入侵菌絲的形成及病害的發展,受到交配型基因所調控,因此本研究首先探討U. esculenta 的交配型及其基因,與有性生殖之誘導。本研究收集臺灣茭白筍主要產區的品種,包括埔里的青殼種、三芝及金山之赤殼種與青殼種,在144株小生子分離株中,59株菌株來自筍體中發芽之厚膜冬孢子,其餘85株則直接分離自植物組織。由誘導厚膜冬孢子發芽,分離減數分裂產生的4個單倍體小生子菌株,經由有性生殖配對試驗,觀察菌絲之生成,確認其交配型為兩兩相同,並且其生殖菌絲與U. maydis型態相似,部份橫隔間細胞具有細胞質中空化的現象,以及前端生殖菌絲部分細胞具有雙核之特性。而為分析茭白筍黑穗菌交配型基因之組成及結構,利用PCR及NGS技術,找到U. esculenta交配型基因位點,目前發現臺灣菌株中,具有三種交配型對偶基因:a1b1、a2b2、a3b3,並且與Sporisorium reilianum黑穗菌的交配型基因位點序列具有高度共線性,其中基因包含2個費洛蒙基因、1個費洛蒙基因接收蛋白質,以及bE與bW基因。另外,在部分基因位點中,亦有Fot1 Family 之跳躍子基因插入。目前,利用PCR技術,大量篩選有性生殖後代菌株及田間分離株的交配型基因,發現子代間或菌株中,交配型對偶基因間並無重組現象,顯示其交配系統可能非原預期之四極異宗型式。而為了進行茭白品種選育工作,亦嘗試進行茭白筍黑穗菌的接種試驗,目前發現青殼品種植株之接種成功率較赤殼植株為高。 | zh_TW |
dc.description.abstract | Zizania latifolia is a perennial aquatic plant. With the infection of smut fungus Ustilago esculenta, swollen stem is developed in Z. latifolia and becomes an edible vegetable, so called water bamboo. Z. latifolia is mainly distributed in China, Japan, and Taiwan. Green shell and red shell are two major cultivars currently grown in Taiwan. Although this crop has been grown for hundreds years, researches related to Z. latifolia and its smut fungus are limited. It is known that the infection process of related smut model fungus, Ustilago maydis, is coupled with sexual differentiation. Development of mating/infectious filaments and disease progression are under control of the mating type locus. Therefore, we aimed to characterize its mating system, mating type loci and induction of sexual development in U. esculenta. In this study, we collected plant materials from the major production areas in Taiwan, including green shell from Puli, red and green shells from Sanchi and Jinshan. In total of 144 U. esculenta strains collected, 59 were isolated from teliospores and 85 were directly isolated from stem tissues. By mating assay, we determined the mating compatibility and defined the mating type. In addition, morphological features of mating hyphae in U. esculenta were characterized. The features such as empty sections in its mating filaments and dikaryons at the front ends of hyphae, which are similar to those in U. maydis, were observed. To determine the mating system and genetic diversity of mating type in U. esculenta, we employed PCR and NGS approaches and identified the mating type locus. Three idiomorphs, including 2 mating pheromones, 1 pheromone receptor, and bW and bE genes were identified from the mating type loci of Taiwan isolates and they shared great synteny conservation with the mating type locus of Sporisorium reilianum. Besides, the Fot1 family transposons were inserted in some idiomrphs of U. esculenta. Idiomorph PCR screening among meiotic offsprings and field isolates found no recombination between idiomorphs, suggesting the heterothallic mating system of U. esculenta may not be tetrapolar as previously thought. In order to breed new variety of water bamboo, we conducted inoculation experiment. Preliminary data showed that green shell showed higher successful rate than red shell. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:58:51Z (GMT). No. of bitstreams: 1 ntu-104-R01633020-1.pdf: 3484926 bytes, checksum: 29946d82e16ace71f524ceeac38e5727 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 致謝 中文摘要 i 英文摘要 ii 目錄 iii 圖目錄 iv 表目錄 v 第一章 研究背景及動機 1 第二章 前人研究 2 2.1 茭白的特徵 2 2.2 茭白與茭白筍黑穗菌之關係 3 2.3 真菌之交配系統 4 2.3.1 同宗及異宗交配(homothallism heterothallism) 4 2.3.2 四極異宗交配系統(tetrapolar heterothallism) 4 2.3.3 二極異宗交配系統(bipolar heterothallism) 5 2.4 黑穗病菌(Smut fungi) 5 2.4.1 黑穗病菌的共同特徵 6 2.4.2 玉米黑穗病菌 Ustilago maydis : 四極異宗交配 6 2.4.3 大麥黑穗病菌 Ustilago hordei : 二極異宗交配 8 2.4.4 茭白筍黑穗菌Ustilago esculenta 9 2.5 黑穗菌基因體之比較 10 2.5.1 U. maydis、S. reilianum 及 U. hordei 之基因體介紹 10 2.5.2 U. maydis、S. reilianum 及 U. hordei 間基因體的比較 10 2.5.3 交配型基因可能之演化模型 11 第三章 材料與方法 12 3.1 菌株分離培養及保存 12 3.2 茭白筍黑穗菌細胞核及橫隔之染色 12 3.3 有性生殖配對 12 3.4 茭白筍黑穗菌DNA的純化 13 3.5 茭白接種試驗 13 3.6 交配型基因的PCR增幅及選殖 14 3.7 多重PCR(multiplex PCR)篩選茭白筍黑穗菌之交配基因型 14 3.8 茭白筍黑穗菌的基因體定序 14 3.8.1 次世代基因體定序DNA的萃取 15 3.8.2 基因庫的建立(library construction) 15 3.8.3 次世代定序 16 3.8.4 生物資訊分析 16 第四章 結果 17 4.1. 臺灣各地茭白植株之採集及其茭白筍黑穗菌之分離 18 4.1.1 臺灣各地茭白植株之採集 18 4.1.2 茭白筍黑穗菌之分離 18 4.2. 茭白筍黑穗菌之生活史 19 4.3. 尋找茭白筍黑穗菌交配型基因之策略 20 4.3.1 PCR增幅反應 20 4.3.2 NGS次世代基因體定序 21 4.3.3 a2b2對偶基因(idiomorph)的發現 21 4.4. 茭白筍黑穗菌A交配型基因位點(A locus) 22 4.5. 茭白筍黑穗菌B交配型基因位點(B locus) 23 4.6. 茭白筍黑穗菌有性生殖後代交配型基因之鑑定 24 4.7. 茭白筍黑穗菌有性生殖配對試驗 25 4.8. 分析Ensembel資料庫及PCR技術,尋找交配型基因位點之初步結果 26 4.9. 茭白筍黑穗菌之接種試驗 26 第五章 討論 27 圖 31 表 46 參考文獻 54 附錄 60 | |
dc.language.iso | zh-TW | |
dc.title | 茭白筍黑穗菌Ustilago esculenta交配型系統及其基因多型性 | zh_TW |
dc.title | Mating system of Ustilago esculenta and its polymorphisms | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 曾顯雄(Shean-Shong Tseng),鍾光仁(Kuang-Ren Chung),黃晉興(Jin-Hsing Haung),王廷方(Ting-Fang Wang) | |
dc.subject.keyword | 茭白筍,茭白筍黑穗菌,異宗交配,交配型基因,接種, | zh_TW |
dc.subject.keyword | Zizania latifolia,water bamboo,Ustilago esculenta,smut fungus,heterothallism,mating type locus,inoculation, | en |
dc.relation.page | 63 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-21 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 3.4 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。