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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 陳中平(Chung-Ping Chen) | |
dc.contributor.author | I-Man Ng | en |
dc.contributor.author | 吳禕文 | zh_TW |
dc.date.accessioned | 2021-06-15T13:52:56Z | - |
dc.date.available | 2016-12-01 | |
dc.date.copyright | 2015-12-01 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-09-14 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51844 | - |
dc.description.abstract | 跳躍基因(亦稱為轉座子)是一類DNA序列,它們能夠從染色體DNA上單獨複製或‘跳躍’出來,再而插入另一位點,因而對插入位點上的基因調控造成影響。PiggyBac跳躍基因是一種從粉紋夜蛾 (cabbage looper moth) 基因組中取得的跳躍基因, 其系統已被廣泛應用在各種哺乳動物細胞系中作為基因組操縱的工具, 已在基因組功能研究和誘導多能幹細胞等領域得到了廣泛的應用。PiggyBac系統的主要特徵包括在不同的物種上有高轉效率,有相對低的插入位點偏好,以及跳離基因體時會不留痕跡。而此研究的對象NP-mPB跳躍酶,則是一種針對核仁的PiggyBac跳躍酶 (PBase),可以通過在哺乳類慣用轉譯碼優化之PiggyBac跳躍酶 (mPB) 上添加來自HIV-1的TAT蛋白訊號多肽來建造。在之前的研究發現NP-mPB跳躍酶可以有效的提升跳躍效率。另外,建造NP-mPB的目的是要透過修改mPB,將跳躍基因引導進核仁組織區(nucleolus organizer regions; NORs),因為在NORs中有很多rDNA 的複本,若進入此區域對基因的造成破壞比較可以避免影響其他基因的正常運作,以達到有效的基因治療。本研究的目的是分析次世代定序(next generation sequencing)的數據,,找出實驗中所有NP-mPB與mPB的插入位點,以顯示其在小鼠基因組的分佈,研究是否有偏向於NORs或其它基因區域的插入趨性。 | zh_TW |
dc.description.abstract | PiggyBac is a popular transposon system used to diver transgenes and explore the unknown genomic territory. PiggyBac transposase (PBase) has been widely applied as a genomic manipulation tool to various mammalian cell lines and model organisms. Major features of the piggyBac system include high transposition efficiency in different species, relatively low insertion site preference, and the ability of seamless removal from genome. These features allow its potential uses in functional genomics in a wide range of organisms, such as plants, cattle, pigs, mice, rats, flies, yeast, and several non-model insects. A novel nucleolus-predominant PBase, NP-mPB, was constructed by adding a nucleolus-predominant (NP) signal peptide from HIV-1 TAT protein to a mammalian codon-optimized PBase (mPB). The initial goal is to create a modified mPB that would increase transposition efficiency and mediate transposition towards the nucleolus organizer regions (NORs), which contains several tandem copies of ribosomal DNA genes. Gene disruption at NORs are believed to be less harmful to the species. This research aims at analyzing raw next generation sequencing (NGS) data of mouse ES cells after being transfected with mPB and NP-mPB. Insertion sites of the two PBs was identified by aligning the processed NGS data to the reference genome. Comparisons of the datasets reveal the transposition preferences of NP-mPB towards NORs and other genome regions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:52:56Z (GMT). No. of bitstreams: 1 ntu-104-R02945042-1.pdf: 2827015 bytes, checksum: 32a0d0ca84619f153b1290754b42f5fe (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vii LIST OF TABLES ix Chapter 1 Introduction 1 1.1 Background 1 1.2 Motivation and Objectives 2 1.3 Significance 3 1.4 Restrictions 4 1.5 Research Procedure 5 1.6 Thesis Organization 6 Chapter 2 Extended Background and Basic Concepts 7 2.1 DNA Transposable Elements 7 2.1.1 Classification of Transposable Elements 7 2.1.2 Transposons-based Applications for Functional Genomics 8 2.1.3 PiggyBac Transposition System 9 2.1.4 A Nucleolus-Predominant piggyBac Transposase NP-mPB 11 2.2 Splinkerette-PCR method 14 2.3 NGS sequencing 16 2.4 Bioinformatics 17 2.4.1 System and Programming 17 2.4.2 Databases and Tools 17 Chapter 3 Data Pre-processing of Raw NGS Data 18 3.1 NGS Raw Data 18 3.1.1 FASTQ and FASTA Format 18 3.1.2 Structure of Single End Reads 19 3.2 Data Pre-processing 20 3.2.1 Pre-processing pipeline 20 3.2.2 Primer and Transposon Sequences Removal 21 3.2.3 Trimming Adaptor Sequences 22 3.2.4 Read Length Selection 23 3.2.5 Grouping Repeat Reads 24 3.2.6 Unique Read Identifier 26 3.3 Data Quality 27 3.4 Data size 29 3.5 Methodology for Target Site Motif analysis 30 Chapter 4 Alignment for Insertion Sites 31 4.1 Sequence Alignment 31 4.1.1 Basic Local Alignment Search Tool 31 4.1.2 Output Format 33 4.2 Databases Selection 33 4.2.1 The Mouse Genome Database 33 4.2.2 Database for the nucleolar organizer regions (NORs) 35 4.2.3 Gene Annotation Database 36 4.3 BLAST Queries and Outputs 37 4.3.1 BLAST for Chromosomal Regions 37 4.3.2 BLAST for NORs 38 4.4 Process Flow for BLAST Results 40 4.4.1 BLAST output 40 4.4.2 Processing pipeline for BLAST Output 42 4.4.3 Total Amount of Repeat Sequences 44 4.4.4 Strategies for Multiple Matches 46 4.4.5 Plotting of Insertion Sites 48 Chapter 5 Statistical Evaluation 49 5.1 Unequal Variances t-test 49 5.2 Poisson Distribution for CIS Analysis 50 5.3 Multiple Hypothesis Correction 51 Chapter 6 Results and Discussion 52 6.1 Target Site Motif Preference of PiggyBac Transposon 52 6.2 Insertions Distributed Throughout Mouse Genome 54 6.2.1 Profile of mPB and NP-mPB Insertion sites 54 6.2.2 Insertions of mPB and NP-mPB at Intragenic Regions 56 6.2.3 Common Insertion Sites of Intragenic Regions 58 6.3 NP-mPB Mediates Insertion Preference towards NORs 60 Chapter 7 Conclusion and Future Work 62 7.1 Conclusions 62 7.2 Future Work 63 BIBLIOGRAPHY 64 | |
dc.language.iso | en | |
dc.title | 以次世代定序結果基因體定位分析NP-mPB跳躍酶的插入點趨性 | zh_TW |
dc.title | Transposition Preference Analysis of a Nucleolus-Predominant PiggyBac Transposase (NP-mPB) by Mapping Next Generation Sequencing- Determined Genomic Insertion Sites | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳佑宗(You-Tzung Chen) | |
dc.contributor.oralexamcommittee | 高成炎(Cheng-yan Kao),賴飛羆(Fei-Pei Lai),李盛安(Sheng-An Li) | |
dc.subject.keyword | 跳躍基因,次世代定序,生醫資訊, | zh_TW |
dc.subject.keyword | PiggyBac,transposon,nucleolus organizer regions,next generation sequencing, | en |
dc.relation.page | 69 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-09-14 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
Appears in Collections: | 生醫電子與資訊學研究所 |
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