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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝學真(Hsyue-Jen Hsieh) | |
| dc.contributor.author | Yu-Tsen Lee | en |
| dc.contributor.author | 李昱岑 | zh_TW |
| dc.date.accessioned | 2021-06-15T13:49:52Z | - |
| dc.date.available | 2020-10-28 | |
| dc.date.copyright | 2015-10-28 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-10-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51789 | - |
| dc.description.abstract | 本研究以靜電紡絲法製備幾丁聚醣/聚氧化乙烯/Eudragit之複合奈米纖維膜；其中Eudragit以乙醇當溶劑配成濃度為12 wt%的溶液，而幾丁聚醣/聚氧化乙烯之溶液則是使用甲、乙酸以7：3的比例作為溶劑溶解幾丁聚醣，再混入已均勻分散於水中的聚氧化乙烯，配成幾丁聚醣/聚氧化乙烯總濃度為5 wt%的混合溶液，因為幾丁聚醣屬於帶正電性的高分子，通常不易單獨電紡成奈米纖維絲，在以4：1的比例搭配聚氧化乙烯後即可順利電紡出穩定的奈米纖維，此方法避免了使用具有高毒性、高腐蝕性的溶劑，使整個製程更加環保且安全。 製備複合奈米纖維主要是利用雙針電紡的方法，分別把幾丁聚醣/聚氧化乙烯之混合物和Eudragit電紡成纖維，因為奈米纖維膜本身非常緻密，不利於物質的透過，主要藉由Eudragit纖維直徑比幾丁聚醣/聚氧化乙烯直徑大上許多的特性，以期可以加大纖維層中的空間，來探討在纖維膜中加入Eudragit纖維是否增大了纖維膜的滲透率，此外，也探討幾丁聚醣/聚氧化乙烯之纖維方向性是否對於滲透率有所影響；在滲透物的選擇方面，選用了體積小的鈣離子以及大分子的牛血清蛋白作為測定膜滲透率之代表因子，目的是利用大小不同的粒子來比較奈米纖維膜滲透率之改善程度；此外，也利用不同水柱壓力差的方式來比較纖維膜的水分子通透率的差異。 根據實驗成果顯示，以雙針電紡所製備出來的幾丁聚醣/聚氧化乙烯/Eudragit之複合奈米纖維膜，在Eudragit纖維的加入下或者使幾丁聚醣/聚氧化乙烯纖維排列具有方向性後，皆能提升纖維膜的滲透率，此種具有較好滲透率的纖維膜若作為細胞生長基材時，主預期將更利於養分的透過來促進細胞生長，未來可望應用於組織工程等相關領域中。 關鍵字：幾丁聚醣、聚氧化乙烯、Eudragit、雙針電紡、滲透率 | zh_TW |
| dc.description.abstract | In this research, a composite nanofiber membrane containing chitosan/poly(ethylene oxide)/Eudragit was fabricated by electrospinning. In preparation of solutions, ethanol was used as solvent to prepare a 12 wt% Eudragit solution, formic and acetic acid at the rate of 7:3 was used as solvent to dissolve chitosan, and then mixed with a well-mixed poly(ethylene oxide) water solution to form a solution of 5 wt%. Due to the positively-charged characteristics of chitosan, it is difficult to fabricate it into nanofiber structure; with the addition of poly(ethylene oxide), stable nanofiber structure could be obtained by electrospinning of chitosan- poly(ethylene oxide) mixture at a 4:1 ratio. This nontoxic and environment-friendly method could replace the using of highly toxic and erosive solvent. A dual-jet electrospinning system was used to fabricate this composite nanofiber membrane; chitosan/poly(ethylene oxide) mixture and Eudragit were separately electrospun. The main reason why Eudragit fibers was fabricated was to enlarge the 3 dimensional space of the nanofiber membrane and to help substances penetrate through the dense nanofiber membrane owing to its larger diameter characteristic. Membrane permeability was regarded as the indicator to verify the results. Besides, the effect of chitosan/poly(ethylene oxide) fiber directionality on membrane permeability was also studied. Calcium ion and bovine serum albumin (BSA) molecule were used as representative factors to determine the membrane permeability in different particle sizes; moreover, water permeation, which was driven by the hydrostatic head, was also taken as an indicator to compare the differences of membrane permeability. According to the experimental results, the permeability of this composite nanofiber membrane was successfully increased by fabricating Eudragit fibers into the membrane and also by changing the directionality of chitosan/poly(ethylene oxide) fibers. Membrane with better permeability would enhance the permeation of nutrients and help cells grow when it is applied to cell culture. These results hold great potential for future applications in tissue engineering-related fields. Key words: chitosan, poly(ethylene oxide), Eudragit, dual-jet electrospinning, permeability | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T13:49:52Z (GMT). No. of bitstreams: 1 ntu-104-R02524090-1.pdf: 7248629 bytes, checksum: c4b357df14200f0fa2bfa11c51e4090d (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄 誌謝 I 摘要 III ABSTRACT V 目錄 VII 表目錄 XI 圖目錄 XIII 符號縮寫說明 XIX 中英文對照表 XXI 1. 緒論 1 1.1. 研究背景與動機 1 1.2. 實驗架構與流程 3 2. 文獻回顧 5 2.1. 組織工程 5 2.2. 奈米纖維材料優缺點 7 2.3. 靜電紡絲法 8 2.3.1. 靜電紡絲原理 8 2.3.2. 靜電紡絲影響因素 11 2.3.2.1. 溶液物理性質 11 2.3.2.2. 靜電紡絲操作參數 14 2.3.2.3. 環境變因 15 2.3.3. 靜電紡絲裝置種類 16 2.3.3.1. 靜電紡絲之針頭設計 16 2.3.3.2. 收集板類別 20 2.3.4. 靜電紡絲法材料種類 22 2.3.4.1. 天然高分子 22 2.3.4.2. 合成高分子 23 2.4. 生醫材料 24 2.4.1. 幾丁聚醣 24 2.4.2. 聚氧化乙烯 26 2.4.3. Eudragit® S-100 27 2.5. 交聯劑 28 3. 實驗材料、儀器與方法 31 3.1. 實驗材料 31 3.2. 實驗儀器 32 3.3. 實驗方法 34 3.3.1. 幾丁聚醣/聚氧化乙烯混合溶液配製 34 3.3.2. Eudragit溶液配製 35 3.3.3. 混合溶液物化性質分析 36 3.3.3.1. 黏度量測 36 3.3.3.2. 導電度量測 36 3.3.4. 靜電紡絲法 37 3.3.4.1. 無特定方向性電紡 37 3.3.4.2. 具特定方向性電紡 39 3.3.5. 纖維膜交聯 40 3.3.6. 纖維膜乙醇清洗 41 3.3.7. 奈米纖維膜之分析 43 3.3.7.1. SEM觀察 43 3.3.7.2. 奈米纖維孔隙度測定 43 3.3.7.3. 機械性質測試-抗拉強度 44 3.3.7.4. FT-IR分析 45 3.3.7.5. TGA分析 45 3.3.8. 電紡纖維膜滲透率探討 46 3.3.8.1. 鈣離子滲透率測定 48 3.3.8.2. 牛血清蛋白滲透率測定 49 3.3.8.3. 水分子通透率測定 50 4. 實驗結果與討論 53 4.1. 溶液性質分析 53 4.1.1. 黏度 53 4.1.2. 導電度 56 4.2. 電紡最佳條件之探討(SEM觀察) 58 4.2.1. 幾丁聚醣/聚氧化乙烯混合比例對於奈米纖維之影響 58 4.2.2. 電壓及流量對於幾丁聚醣/聚氧化乙烯奈米纖維之影響 63 4.2.3. Eudragit濃度及流量對奈米纖維之影響 66 4.2.4. 電壓及流量對於複合奈米纖維之影響 70 4.3. 收集板轉速及轉向之探討 72 4.3.1. 收集板轉速對於電紡纖維型態之影響 74 4.3.2. 收集板轉向對於電紡纖維膜厚之影響 77 4.4. 交聯時間與乙醇清洗時間對於奈米纖維膜之影響(SEM觀察) 78 4.5. 機械性質測試 81 4.6. 奈米纖維膜膜厚測定 86 4.7. 奈米纖維膜孔隙率測定 87 4.8. 滲透率測定 88 4.8.1. 鈣離子之滲透 89 4.8.2. 牛血清蛋白(BSA)之滲透 96 4.8.3. 水分子 100 4.9. 材料物理性質測定- FT-IR分析 103 4.10. 熱性質分析-TGA分析 105 5. 結論與未來研究方向 107 5.1. 結論 107 5.2. 未來研究方向 110 6. 參考文獻 111 | |
| dc.language.iso | zh-TW | |
| dc.subject | Eudragit | zh_TW |
| dc.subject | 滲透率 | zh_TW |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | 聚氧化乙烯 | zh_TW |
| dc.subject | 雙針電紡 | zh_TW |
| dc.subject | dual-jet electrospinning | en |
| dc.subject | Eudragit | en |
| dc.subject | poly(ethylene oxide) | en |
| dc.subject | chitosan | en |
| dc.subject | permeability | en |
| dc.title | 幾丁聚醣/聚氧化乙烯暨Eudragit電紡纖維膜之製備及特性探討 | zh_TW |
| dc.title | Fabrication and Characterization of Chitosan/ Polyethylene Oxide and Eudragit Composite Nanofiber Membranes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 何明樺,胡晉嘉 | |
| dc.subject.keyword | 幾丁聚醣,聚氧化乙烯,Eudragit,雙針電紡,滲透率, | zh_TW |
| dc.subject.keyword | chitosan,poly(ethylene oxide),Eudragit,dual-jet electrospinning,permeability, | en |
| dc.relation.page | 119 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-10-22 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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