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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃青真 | |
| dc.contributor.author | Jia-Wei Lin | en |
| dc.contributor.author | 林家暐 | zh_TW |
| dc.date.accessioned | 2021-06-15T13:49:37Z | - |
| dc.date.available | 2018-12-01 | |
| dc.date.copyright | 2015-12-01 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-10-21 | |
| dc.identifier.citation | 廖運文，苦瓜莖部與諾麗心材化學成分及生物活性之研究。2006，國立屏東科技
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51784 | - |
| dc.description.abstract | 能量失衡是造成肥胖的主因，肥胖容易增加糖尿病、心血管等代謝疾病風險危及健康，並且肥胖盛行率逐年提高，已成為全球重要的公衛議題。苦瓜 (Momordica charantia L.) 為產於熱帶亞洲地區之日常蔬果，已有文獻證實苦瓜具有降血糖、降血脂、對抗肥胖與糖尿病等代謝異常疾病之功效。近期本實驗室研究發現，長期餵食苦瓜之C57BL/6J小鼠，骨骼肌粒線體生合成相關基因Pgc1a、Nrf1、Tfam表現增加，並且體脂肪較少、個體能量消耗較高。因此本篇研究目標將以L6與C2C12肌肉細胞為模式，探討山苦瓜萃取物對肌肉細胞粒線體增殖與功能之影響。
第一部分實驗以咖啡因作為正控制組建立L6與C2C12肌肉細胞粒線體增殖與功能提升之模式，發現咖啡因處理24小時可顯著增加L6與C2C12肌肉細胞粒線體生合成相關基因表現，檸檬酸合成酶 (CS) 活性提升，粒線體DNA複本數較高。第二部分實驗以山苦瓜乙酸乙酯萃取物 (EAE)、氯仿粗萃物酸水解後正己烷萃取物 (HEX) 與未酸水解之HEX(un) 處理細胞，發現EAE、HEX、HEX(un) 皆顯著增加肌肉細胞CS活性，但不影響粒線體生合成相關基因，顯示山苦瓜萃取物在不促進細胞粒線體的情況下提升粒線體CS活性。第二部分另比較存在苦瓜中之熊果酸 (UA) 對肌肉細胞粒線體之影響，結果顯示UA能促進L6肌肉細胞CS活性、粒線體生合成相關基因表現，增加細胞氧氣消耗速率，在C2C12肌肉細胞則否。 綜合以上，山苦瓜萃取物在本模式下雖不使肌肉細胞粒線體增殖，但可以促進粒線體CS酵素活性提升，細胞耗氧量增加。而苦瓜當中所含之成分熊果酸，能夠促進L6肌肉細胞CS酵素活性提升、粒線體增殖與增加細胞氧氣消耗。因此山苦瓜實具有活化粒線體功能之潛力，以改善肥胖等代謝異常疾病。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T13:49:37Z (GMT). No. of bitstreams: 1 ntu-104-R02b22006-1.pdf: 3274165 bytes, checksum: 93f7626a2a78fbb3091001287ec47c25 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract iii 縮寫對照表 v 總目錄 viii 圖目錄 xii 表目錄 xv 第一章 緒論 1 第一節 前言 1 第二節 文獻回顧 2 一、 肥胖與代謝症候群 2 二、 糖尿病 3 三、 肌肉組織與代謝異常 4 四、 粒線體 5 4.1粒線體的功能 6 4.2粒線體的功能缺失與代謝疾病 7 4.3粒線體的分裂與融合 9 4.4粒線體生合成與調控之相關基因 11 五、 山苦瓜 15 5.1咖啡因 15 5.2苦瓜與三萜類化合物 16 5.4熊果酸與齊墩果酸 17 5.3苦瓜與抗肥胖 18 第三節 研究假說與實驗架構 25 一、 研究假說 25 二、 研究架構 26 第二章 促進肌肉細胞粒線體增殖之模式建立 27 第一節 前言與實驗架構 27 一、 前言 27 二、 實驗架構 28 第二節 材料與方法 29 一、 細胞培養 29 1.1細胞株 29 1.2培養基與藥品試劑 29 1.3方法步驟 30 二、 藥品試劑與藥品處理 31 三、 MTT assay 31 四、 檸檬酸合成酶活性測定 32 4.1樣品製備 32 4.2藥品試劑 32 4.3原理 32 4.4方法步驟 33 五、 Real-time PCR分析基因表現與粒線體複本數 34 5.1總RNA抽取 34 5.2 RNA反轉錄為cDNA 34 5.3總DNA抽取 34 5.4 Real-time PCR分析基因表現 35 六、 海馬生物能量分析儀分析細胞氧氣消耗與產酸速率 36 6.1細胞培養 36 6.2螢光探針活化與校正 36 6.3細胞氧氣消耗與產酸速率分析 36 七、 儀器設備 37 八、 統計分析 37 第三節 結果 38 一、 咖啡因處理細胞5天，每天5小時 38 1.1 CS活性分析 38 1.2粒線體生合成相關基因表現分析 38 二、 咖啡因處理細胞24小時 38 2.1 MTT assay 39 2.2 CS活性分析 39 2.3粒線體相關基因表現分析 39 2.4粒線體DNA複本數分析 40 2.5細胞氧氣消耗速率 (OCR) 與產酸速率 (ECAR) 分析 40 第四節 討論 49 第五節 結論 50 第三章 山苦瓜萃取物對L6與C2C12肌肉細胞粒線體增殖與功能之影響 51 第一節 前言與實驗架構 51 一、 前言 51 二、 實驗架構 53 第二節 材料與方法 54 一、 細胞培養 54 二、 山苦瓜樣品製備與樣品處理 54 2.1 山苦瓜樣品製備 54 2.2樣品處理 55 三、 MTT assay 55 四、 檸檬酸合成酶活性測定 55 五、 Real-time PCR分析基因表現與粒線體複本數 56 六、 海馬生物能量分析儀分析細胞氧氣消耗與產酸速率 56 七、 薄層層析 56 八、 總三萜類呈色法測定 56 九、 山苦瓜萃取物三酸甘油酯含量測定 57 9.1原理 57 9.2方法步驟 57 十、 儀器設備 58 十一、 統計分析 58 第三節 結果 59 一、 山苦瓜萃取物處理細胞24小時 59 1.1 MTT assay 59 1.2 CS活性分析 59 1.3粒線體相關基因表現分析 60 1.4粒線體DNA複本數分析 60 二、 熊果酸、齊墩果酸處理細胞24小時 61 2.1 MTT assay 61 2.2 CS活性 61 2.3 粒線體相關基因表現 61 2.4粒線體DNA複本數 62 2.5細胞氧氣消耗速率 (OCR) 與產酸速率 (ECAR) 分析 62 三、 山苦瓜萃取物、熊果酸處理細胞4天 62 3.1 CS活性分析 62 3.2 粒線體相關基因表現量分析 63 四、 分析山苦瓜樣品中之組成 63 4.1 TLC薄層層析 63 4.2 HEX與HEX(un) 總三萜類含量分析與山苦瓜萃取物總三酸甘油酯含量分析 64 第四節 討論 87 第五節 結論 90 第四章 綜合討論與總結論 91 第一節 綜合討論 91 第二節 總結論 92 第五章 參考文獻 95 附錄 110 | |
| dc.language.iso | zh-TW | |
| dc.subject | 肌肉細胞 | zh_TW |
| dc.subject | 粒線體 | zh_TW |
| dc.subject | 檸檬酸合成? | zh_TW |
| dc.subject | 山苦瓜 | zh_TW |
| dc.subject | myotube | en |
| dc.subject | mitochondria | en |
| dc.subject | Bitter gourd | en |
| dc.subject | citrate synthase | en |
| dc.title | 初探山苦瓜萃取物對L6與C2C12肌肉細胞粒線體增殖與功能之影響 | zh_TW |
| dc.title | An initial approach to explore the effects of Momordica chanratia L. extracts on mitochondrial biogenesis and functions in L6 and C2C12 myotubes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 呂紹俊,蘇慧敏,林甫容,張美鈴 | |
| dc.subject.keyword | 山苦瓜,肌肉細胞,粒線體,檸檬酸合成?, | zh_TW |
| dc.subject.keyword | Bitter gourd,mitochondria,myotube,citrate synthase, | en |
| dc.relation.page | 114 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-10-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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