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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 施明哲(Ming-Che Shih) | |
| dc.contributor.author | Sang-Chu Lin | en |
| dc.contributor.author | 林桑竹 | zh_TW |
| dc.date.accessioned | 2021-06-15T13:32:25Z | - |
| dc.date.available | 2016-03-08 | |
| dc.date.copyright | 2016-03-08 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-02-02 | |
| dc.identifier.citation | Acheson, S.A., Kirkman, H.N., and Wolfenden, R. (1988). Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration. Biochemistry 27, 7371-7375.
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NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences. Antioxid. Redox Signal. 10, 179-206. Yoshida, A., and Dave, V. (1975). Inhibition of NADP-dependent dehydrogenases by modified products of NADPH. Arch. Biochem. Biophys. 169, 298-303. Zeidler, J., Gupta, R.N., Sayer, B.G., and Spenser, I.D. (2003). Biosynthesis of Vitamin B(6) in yeast. Incorporation pattern of trioses. J. Org. Chem. 68, 3486-3493. Zein, F., Zhang, Y., Kang, Y.N., Burns, K., Begley, T.P., and Ealick, S.E. (2006). Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima. Biochemistry 45, 14609-14620. Zhao, G., and Winkler, M.E. (1995). Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12. J. Bacteriol. 177, 883-891. Zhao, G., and Winkler, M.E. (1996). 4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12. FEMS Microbiol. Lett. 135, 275-280. Zhu, J., Burgner, J.W., Harms, E., Belitsky, B.R., and Smith, J.L. (2005). A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase. J. Biol. Chem. 280, 27914-27923. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51384 | - |
| dc.description.abstract | 穀氨醯胺(Glutamine)是植物體內含量最豐富的胺基酸之一，做為初級氮同化作用最初始的有機氮產物，也是合成其他胺基酸、核酸、葉綠素等含氮化合物時氮的提供來源。我們發現以glutamine作為唯一氮源時，阿拉伯芥可以有效的吸收利用以供植物生長發育，但過量的glutamine則會抑制植物生長，因此我們使用能使野生種正常生長的glutamine濃度，篩選對glutamine過度敏感而出現生長抑制的突變株。突變株23844/pdx3-3為其中之一，在以glutamine作為唯一氮源時出現主根生長被顯著抑制、細胞大小排列異常、細胞分裂數量較少且在根部表面出現不正常的細胞凋亡等性狀，最終造成根系發育受到影響。根據map-based cloning及next generation sequencing定位pdx3-3之突變基因，發現一個點突變在At5g49970基因上，使Gln226轉換成終止密碼子，影響了維生素B6生合成補救途徑(vitamin B6 salvage pathway)中pyridoxine/ pyridoxamine phosphate oxidase (PDX3/PPOX)的表現。從遺傳互補及基因靜默植株的性狀觀察中，顯示pdx3-3對glutamine過度敏感的性狀確實來自於PDX3失去功能所造成。在另一個維生素B6補救途徑突變株sos4 (salt overly sensitive 4)也觀察到和pdx3-3相似的性狀，而在pdx3-3/sos4雙重突變株有性狀加乘的效果，此外在pdx3-3及sos4中則有維生素B6衍生物含量不正常累積的現象，顯示維生素B6補救途徑的缺失會導致對glutamine過度敏感的性狀。由於PDX3及維生素B6補救途徑的功能尚未被完全了解透徹，因此我們會再進一步研究glutamine和維生素B6補救途徑的關係，以及對維生素B6恆定的調控。 | zh_TW |
| dc.description.abstract | Glutamine is one of the most abundant free amino acids in plants. As the first organic nitrogen from primary nitrogen assimilation, glutamine is a major amino donor for the synthesis of amino acids, nucleotides, and other nitrogen-containing compounds. We showed that glutamine could efficiently support Arabidopsis growth when used as a sole nitrogen source in the growth medium, but the addition of excess glutamine significantly inhibited Arabidopsis growth. We thus used appropriate glutamine concentration as sole nitrogen source to screen for “glutamine hypersensitive” mutants. One of the mutants, line 23844/pdx3-3, had dramatically reduced root growth, irregular cell arrangement, less cell proliferation and abnormal cell death in glutamine-containing medium. Line 23844 is defective in At5g49970 that encodes pyridoxine/ pyridoxamine phosphate oxidase (PDX3) of the vitamin B6 salvage pathway. The mutant has a point mutation that changes Gln226 to a stop codon. In addition to the mutant, we have obtained the genetic complementation line and PDX3 gene silencing lines that show similar phenotypes. These results suggested that the growth defects in 23844 are caused by loss-of-function in the PDX3 gene. sos4 (salt overly sensitive 4), another mutant involved in vitamin B6 salvage pathway, had similar phenotypes with pdx3-3, and pdx3-3/sos4 double mutant had more severe phenotypes. Furthermore, by analysis vitamin B6 contents in pdx3-3 and sos4, we found abnormal vitamin B6 levels in mutants. These data indicated that defects in vitamin B6 salvage pathway might result in glutamine hypersensitive phenotypes. Nevertheless, the functions of PDX3 and the vitamin B6 salvage pathway are not well understood in plants. We will further study the relationship between glutamine and vitamin B6 salvage pathway, and the regulation of vitamin B6 homeostasis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T13:32:25Z (GMT). No. of bitstreams: 1 ntu-105-R02b42005-1.pdf: 3618111 bytes, checksum: e98359ae290e14b7891b7eb8f1b4e326 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 i 誌謝 ii 中文摘要 iii ABSTRACT iv 目錄 v 圖目錄 x 附錄目錄 xi 第一章 前言 1 1. 初級氮同化作用 (Primary nitrogen assimilation) 1 2. 轉氨作用(Transamination) 2 3. 磷酸吡哆醛(pyridoxal 5’-phosphate, PLP) 3 4. 維生素B6 (vitamin B6) 4 5. 維生素B6生合成機制 5 5.1 de novo pathway 5 5.1.1 DXP-dependent de novo pathway 5 5.1.2 DXP-independent de novo pathway 6 5.2 Salvage pathway 7 6. 前人研究及研究動機 9 第二章 材料與方法 10 1. 植物材料及栽培條件 10 2. 葉綠素含量分析 10 3. 花青素含量分析 11 4. Propidium iodide染色 11 5. EdU染色 11 6. Evans blue染色 11 7. 阿拉伯芥DNA萃取 12 8. 聚合酶連鎖反應(Polymerase Chain Reaction, PCR) 12 9. DNA瓊脂糖凝膠電泳(DNA gel electrophoresis) 13 10. DNA純化 13 11. Map-based cloning 14 12. 次世代定序( Next generation sequencing, NGS) 14 13. T-DNA插入突變株pdx3-1之基因型鑑定(Genotyping) 14 14. RNA定量分析 15 14.1阿拉伯芥RNA萃取 15 14.2 DNase處理 15 14.3 反轉錄反應(Reverse Transcription) 16 14.4即時定量聚合酶連鎖反應(Quantitative Real-Time Polymerase Chain Reaction, qRT-PCR) 16 15. 選殖技術(Cloning) 17 15.1 TOPO Cloning 17 15.2 大腸桿菌轉型作用(Transformation) 17 15.3 抽取質體DNA 17 15.4 LR reaction 18 15.5 農桿菌轉型作用 18 16. 阿拉伯芥轉殖株製備及篩選 19 16.1 Flower dipping 19 16.2 阿拉伯芥轉殖株篩選 19 17. anti-PDX3多株抗體製備 20 17.1 限制酶水解實驗(Restriction enzyme digestion) 20 17.2 DNA接合(DNA ligation) 20 17.3 純化PDX3重組蛋白 20 17.4 抗體代製 21 18. 蛋白質表現分析 21 18.1阿拉伯芥蛋白質萃取 21 18.2 蛋白質定量 21 18.3 蛋白質膠體電泳(SDS-PAGE) 22 18.4 Coomassie Blue染色 22 18.5 西方墨點法 22 19. pdx3-3/sos4雙重突變株之建立及基因型鑑定 23 20. 阿拉伯芥維生素B6含量測定 23 第三章 結果 24 1. 不同濃度glutamine對阿拉伯芥生長之影響 24 2. 篩選對glutamine敏感之突變株23844 26 3. 比較不同氮源處理下突變株23844之根部型態 27 4. 以EdU染色技術比較突變株23844之根尖細胞分裂能力 27 5. 突變株23844以glutamine作為氮源時根部出現細胞凋亡的性狀 29 6. 不同濃度glutamine對突變株23844之影響 30 7. 以map-based cloning及次世代定序技術定位突變株23844之突變基因 31 8. 比較突變株23844及weak allele, pdx3-1性狀之差異 32 9. 比較突變株23844及pdx3-1內PDX3基因及蛋白質表現量 33 10. 以35S: PDX3遺傳互補突變株23844 (pdx3-3)之性狀 34 11. 基因靜默植株出現和pdx3-3相似的性狀 35 12. 外加維生素B6無法化學互補pdx3-3之性狀 36 13. pdx3-3和另一個salvage pathway突變株sos4具有相似的性狀 38 14. 觀察pdx3-3及sos4在土壤中的性狀 39 15. 測定pdx3-3及sos4中維生素B6含量 39 第四章 討論 40 1. 對glutamine過度敏感突變株pdx3-3影響維生素B6補救途徑(salvage pathway) 40 2. Salvage pathway的功能 41 3. 阿拉伯芥PDX3具有PPOX domain及epimerase domain 42 4. 維生素B6 salvage pathway與植物荷爾蒙的關聯 44 參考文獻 45 附錄 55 圖目錄 圖一、植物體內初級氮同化作用 55 圖二、維生素B6生合成途徑 56 圖三、不同胺基酸作為氮源對阿拉伯芥幼苗生長的影響 57 圖四、不同濃度硝酸銨或glutamine對阿拉伯芥幼苗生長的影響 58 圖五、突變株23844以NH4NO3或glutamine做為氮源之植株性狀 60 圖六、突變株23844以NH4NO3或glutamine做為氮源之根尖細胞型態 61 圖七、突變株23844以NH4NO3或glutamine做為氮源之EdU染色結果 62 圖八、突變株23844以NH4NO3或glutamine做為氮源之Evans blue染色結果 63 圖九、不同濃度glutamine做為氮源對突變株23844之影響 64 圖十、定位突變株23844突變基因位置 65 圖十一、比較突變株23844及pdx3-1性狀 66 圖十二、突變株23844及pdx3-1中PDX3基因及蛋白質表現 67 圖十三、以35S: PDX3互補突變株23844性狀 68 圖十四、過度表達PDX3之轉基因植物性狀 69 圖十五、基因靜默(gene silencing)植株之性狀 70 圖十六、外加維生素B6對pdx3-3的影響 71 圖十七、sos4及pdx3-3/sos4以NH4NO3或glutamine做為氮源之植株性狀 72 圖十八、pdx3-3, PDX3基因靜默品系及sos4於土壤中的性狀 73 圖十九、pdx3-3 及sos4維生素B6含量測定實驗 74 圖二十、阿拉伯芥PDX3示意圖 75 附錄目錄 附錄一、引子使用對照表 76 附錄二、TOPO cloning使用之載體 pENTRTM/D-TOPO 78 附錄三、重組蛋白使用之載體 pQE-30 79 | |
| dc.language.iso | zh-TW | |
| dc.subject | 穀氨醯胺 | zh_TW |
| dc.subject | 維生素B6補救途徑 | zh_TW |
| dc.subject | PDX3 | zh_TW |
| dc.subject | sos4 | zh_TW |
| dc.subject | pdx3-3 | zh_TW |
| dc.subject | 穀氨醯胺 | zh_TW |
| dc.subject | 維生素B6補救途徑 | zh_TW |
| dc.subject | PDX3 | zh_TW |
| dc.subject | sos4 | zh_TW |
| dc.subject | pdx3-3 | zh_TW |
| dc.subject | Glutamine | en |
| dc.subject | pdx3-3 | en |
| dc.subject | sos4 | en |
| dc.subject | Glutamine | en |
| dc.subject | pdx3-3 | en |
| dc.subject | sos4 | en |
| dc.subject | PDX3 | en |
| dc.subject | vitamin B6 salvage pathway | en |
| dc.subject | PDX3 | en |
| dc.subject | vitamin B6 salvage pathway | en |
| dc.title | 阿拉伯芥中穀氨醯胺對維生素B6生合成途徑的影響 | zh_TW |
| dc.title | Effects of Glutamine on Vitamin B6 Biosynthesis in Arabidopsis thaliana | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 謝明勳(Ming-Hsiun Hsieh) | |
| dc.contributor.oralexamcommittee | 林讚標,張英?,常怡雍 | |
| dc.subject.keyword | 穀氨醯胺,維生素B6補救途徑,PDX3,sos4,pdx3-3, | zh_TW |
| dc.subject.keyword | Glutamine,vitamin B6 salvage pathway,PDX3,sos4,pdx3-3, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-02-02 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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