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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 鄧麗珍 | |
| dc.contributor.author | Xiao-Mei Wang | en |
| dc.contributor.author | 王曉梅 | zh_TW |
| dc.date.accessioned | 2021-06-15T12:53:50Z | - |
| dc.date.available | 2026-12-31 | |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-07-18 | |
| dc.identifier.citation | 1. Lowy FD: Staphylococcus aureus Infections. New Engl J Med 1998, 339(8):520-532.
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Ruimy R, Armand-Lefevre L, Barbier F, Ruppe E, Cocojaru R, Mesli Y, Maiga A, Benkalfat M, Benchouk S, Hassaine H et al: Comparisons between geographically diverse samples of carried Staphylococcus aureus. J Bacteriol 2009, 191(18):5577-5583. 11. Ruimy R, Angebault C, Djossou F, Dupont C, Epelboin L, Jarraud S, Lefevre LA, Bes M, Lixandru BE, Bertine M et al: Are host genetics the predominant determinant of persistent nasal Staphylococcus aureus carriage in humans? J Infect Dis 2010, 202(6):924-934. 12. Jenney A, Holt D, Ritika R, Southwell P, Pravin S, Buadromo E, Carapetis J, Tong S, Steer A: The clinical and molecular epidemiology of Staphylococcus aureus infections in Fiji. BMC Infect Dis 2014, 14:160. 13. Monecke S, Stieber B, Roberts R, Akpaka PE, Slickers P, Ehricht R: Population structure of Staphylococcus aureus from Trinidad & Tobago. PLoS One 2014, 9(2):e89120. 14. Argudín M, Dodémont M, Vandendriessche S, Rottiers S, Tribes C, Roisin S, de Mendonça R, Nonhoff C, Deplano A, Denis O: Low occurrence of the new species Staphylococcus argenteus in a Staphylococcus aureus collection of human isolates from Belgium. Eur J Clin Microbiol Infect Dis 2016, 35(6):1017-1022. 15. Chantratita N, Wikraiphat C, Tandhavanant S, Wongsuvan G, Ariyaprasert P, Suntornsut P, Thaipadungpanit J, Teerawattanasook N, Jutrakul Y, Srisurat N et al: Comparison of community-onset Staphylococcus argenteus and Staphylococcus aureus sepsis in Thailand: a prospective multicentre observational study. Clin Microbiol Infect 2016. 16. Tong SY, Sharma-Kuinkel BK, Thaden JT, Whitney AR, Yang SJ, Mishra NN, Rude T, Lilliebridge RA, Selim MA, Ahn SH et al: Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin. J Infect Dis 2013, 208(3):520-527. 17. 李浩: 非典型dnaJ基因型金黃色葡萄球菌之特性探討. 2014. 18. 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Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B: Interpreting Chromosomal DNA Restriction Patterns Produced by Pulsed-Field Gel-Electrophoresis - Criteria for Bacterial Strain Typing. Journal of clinical microbiology 1995, 33(9):2233-2239. 34. van Wamel WJ, Rooijakkers SH, Ruyken M, van Kessel KP, van Strijp JA: The innate immune modulators staphylococcal complement inhibitor and chemotaxis inhibitory protein of Staphylococcus aureus are located on beta-hemolysin-converting bacteriophages. J Bacteriol 2006, 188(4):1310-1315. 35. Grumann D, Nubel U, Broker BM: Staphylococcus aureus toxins--their functions and genetics. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2014, 21:583-592. 36. Uchiyama T, Imanishi K, Miyoshi-Akiyama T, Kato H: Staphylococcal superantigens and the diseases they cause. The comprehensive sourcebook of bacterial protein toxins 2006:830-843. 37. Marr JC, Lyon JD, Roberson JR, Lupher M, Davis WC, Bohach GA: Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications. Infect Immun 1993, 61(10):4254-4262. 38. Salgado-Pabón W, Herrera A, Vu BG, Stach CS, Merriman JA, Spaulding AR, Schlievert PM: Staphylococcus aureus β-toxin Production is Common in Strains with the β-toxin Gene Inactivated by Bacteriophage. J Infect Dis 2014:jiu146. 39. Carfora V, Caprioli A, Marri N, Sagrafoli D, Boselli C, Giacinti G, Giangolini G, Sorbara L, Dottarelli S, Battisti A: Enterotoxin genes, enterotoxin production, and methicillin resistance in Staphylococcus aureus isolated from milk and dairy products in Central Italy. Int Dairy J 2015, 42:12-15. 40. Baba T, Takeuchi F, Kuroda M, Yuzawa H, Aoki K-i, Oguchi A, Nagai Y, Iwama N, Asano K, Naimi T: Genome and virulence determinants of high virulence community-acquired MRSA. The Lancet 2002, 359(9320):1819-1827. 41. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50708 | - |
| dc.description.abstract | 金黃色葡萄球菌一般具有能夠合成金黃色葡萄球菌色素”staphyloxanthin”的基因，但少數為缺乏色素，澳洲Giffard研究團隊分析缺乏色素菌株─MSHR1132，並在西元2015年正式將其發表名為Staphylococcus argenteus之新菌種。S. argenteus在台灣還未有相關的研究報導；本實驗室發現早在西元2000年(0.7%)、2005年(3%)時此菌群就已存在，在近年2010年(13%)、2011年(17%)和2012年(16.5%)有增加的趨勢且維持相當比例；本研究分析發現96株dnaJ不典型且缺乏色素菌株，進一步確認為S. argenteus。共可分成五種MLST型別，分別是ST2250、ST2793、ST1223、ST2198和Unknown type，其中以ST2250占最多比例有74%(71 of 96)；ST2250特別的是擁有和MSHR1132極為相似的CRISPR/Cas免疫系統，以及能夠依spacer種類和數量分為不同CRISPR型別，我們也觀察到一些spacer會與特定direct repeat一起增減之現象，推測應存在著某種特殊機制並或許有助於基因編輯工具的發展。我們使用16S rRNA序列比對與nuc PCR皆無法區分S. argenteus和S. aureus；MALDI-TOF MS的分析顯示兩者確實有不同的表現。目前臨床鑑定還未能將兩者區分，本實驗室發現以dnaJ PCR-RFLP的鑑定方法能快速且正確分辨S. argenteus和S. aureus菌株。 | zh_TW |
| dc.description.abstract | Most Staphylococcus aureus have the genes for production of staphyloxanthin, but some don’t. A new species lacking pigment designated as Staphylococcus argenteus sp. nov. was published in 2015. It has not been reported in Taiwan yet. We found that S. argenteus isolate has existed in 2000 (0.7%) in Northern Taiwan, followed by 5 (3%) isolates in 2005, and 29 isolates (13%) in 2010. The isolation of it continued in 2011 (17%) and 2012 (16.5%). In this study, we analyzed 96 S. argenteus that could be divided into five MLST types: ST2250, ST2793, ST1223, ST2198 and one unknown ST type. ST2250 is the major clone of these isolates, presents in 74% (71 of 96). In addition, we found ST2250 is unique because all of it possesses the CRISPR/Cas system. And it can be designated as different CRISPR types according to the arrangement of direct repeat (DR) and spacer variants. We also observed the certain spacer is cross-linked to specific DR, results in a set of “spacer-DR” disappeared or integrated into some types. It was proposed that there must be an unknown and special mechanism on CRISPR/Cas system in ST2250 and may be helpful to develop more convinent tool for genome editing in the future. On other hand, S. argenteus can’t be distinguished from S. aureus by 16S rRNA or nuc PCR. We also performed MALDI-TOF MS analysis to determine their different profiles. Most impotantly, we found that dnaJ PCR-RFLP is a fast and correct method can be used to differentiate between S. argenteus and S. aureus in this study. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T12:53:50Z (GMT). No. of bitstreams: 1 ntu-105-R03424011-1.pdf: 2550910 bytes, checksum: 1e43655a663c56cfb8e07b73c4829978 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員會審定書
誌謝 I 中文摘要 II 英文摘要 III 目錄 V 圖目錄 VI 表目錄 VII 第一章 緒論 1 第二章 研究目的 3 第三章 實驗材料及方法 4 第四章 結果 24 第五章 討論 32 第六章 附圖 38 第七章 附表 45 參考文獻 61 | |
| dc.language.iso | zh-TW | |
| dc.subject | CRISPR/Cas | zh_TW |
| dc.subject | 金黃色葡萄球菌 | zh_TW |
| dc.subject | dnaJ | zh_TW |
| dc.subject | 金黃色葡萄球菌 | zh_TW |
| dc.subject | 銀色葡萄球菌 | zh_TW |
| dc.subject | 銀色葡萄球菌 | zh_TW |
| dc.subject | CRISPR/Cas | zh_TW |
| dc.subject | 質譜儀 | zh_TW |
| dc.subject | dnaJ | zh_TW |
| dc.subject | 質譜儀 | zh_TW |
| dc.subject | MALDI-TOF | en |
| dc.subject | Staphylococcus argenteus | en |
| dc.subject | Staphylococcus aureus | en |
| dc.subject | dnaJ | en |
| dc.subject | CRISPR/Cas | en |
| dc.subject | MALDI-TOF | en |
| dc.subject | Staphylococcus argenteus | en |
| dc.subject | Staphylococcus aureus | en |
| dc.subject | dnaJ | en |
| dc.subject | CRISPR/Cas | en |
| dc.title | 北台灣無色素金黃色葡萄球菌之
特性探討 | zh_TW |
| dc.title | Characterization of Non-pigmented Staphylococcus aureus in Nothtern Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖淑貞,邱浩傑,洪薇鈞 | |
| dc.subject.keyword | 銀色葡萄球菌,金黃色葡萄球菌,dnaJ,CRISPR/Cas,質譜儀, | zh_TW |
| dc.subject.keyword | Staphylococcus argenteus,Staphylococcus aureus,dnaJ,CRISPR/Cas,MALDI-TOF, | en |
| dc.relation.page | 66 | |
| dc.identifier.doi | 10.6342/NTU201600923 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-07-19 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
| Appears in Collections: | 醫學檢驗暨生物技術學系 | |
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| ntu-105-1.pdf Restricted Access | 2.49 MB | Adobe PDF |
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