請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50268
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 郭宗甫 | |
dc.contributor.author | Yu-Hao Liu | en |
dc.contributor.author | 劉宇豪 | zh_TW |
dc.date.accessioned | 2021-06-15T12:34:28Z | - |
dc.date.available | 2021-08-24 | |
dc.date.copyright | 2016-08-24 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-01 | |
dc.identifier.citation | Agung M, Ochi M, Yanada S, Adachi N, IzutaY, Yamasaki T, Toda K. Mobilization of bone marrow-derived mesenchymal stem cells into the injured tissues after intraarticular injection and their contribution to tissue regeneration. Knee Surg Sports Traumatol Arthrosc 14:1307-1314, 2006.
Anitua, E., Prado, R., & Orive, G. (2013). Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics. Trends Biotechnol, 31(6), 364-374. Doi: 10.1016/j.tibtech.2013.04.003. Arthur A, Rychkov G, Shi S, Koblar SA, Gronthos S. Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem cells. 2008;26:1787-95. Bain JR, Mackinnon SE, Hudson AR, et al. The peripheral nerve allograft in the primate immunosuppressed with Cyclosporin A: I. Histologic and electrophysiologic assessment. Plast Reconstr Surg 1992;90:1036e46. Barry F, Boynton RE, Liu B, Murphy JM. Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components. Exp Cell Res 268: 189-200, 2001. Barry FP. Biology and clinical applications of mesenchymal stem cells. Birth Defects Res C Embryo Today 69: 250-6, 2003. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. The international journal of biochemistry & cell biology. 2004;36:568-84. Blair Price, Flaumenhaft Robert . Platelet a-granules: Basic biology and clinical correlates. Blood Reviews 23 177–189, 2009. Burnouf T, Su CY, Radosevich M, Goubran H, El-Ekiaby M. Blood-derived 29 biomaterials: fibrin sealant, platelet gel and platelet fibrin glue. SBT Science Series 4, 136–142, 2009. Caplan AI. Mesenchymal stem cells. Handbook of Stem Cell 299-308, 2004. Choukroun, J., Diss, A. Simonpieri,A., Girard, M. O., Schoeffler, C., Dohan, S. L.,….Dohan, D. M. (2006). Platelet-rich fibrin (PRF) : a second-generation platelet concentrate. Oral Surg OralMed Oral Pathol Oral Radiol Endod, 101(3), e56-60. Doi: 10.1016/j.tripleo.2005.07.011 Chung TY, Chiu FY, Tsai YA, et al. The comparison of eletrophysiologic findings of traumatic brachial plexopathies in a teriary care center. Injury 33,591-595, 2002. Damien P. Kuffler. An assessment of current techniques for inducing axon regeneration and neurological recovery following peripheral nerve trauma. Progress in Neurobiology 116 (2014) 1–12. David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly, DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:E37-44 David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly, DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:E45-50 David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly, DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part III: Leucocyte activation: A new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:E51-5. David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly, DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:E56-60 David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly, DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303 David M. Dohan, DDS, MS, Joseph Choukroun, MD, Antoine Diss, DDS, MS, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, DDS, PhD, and Bruno Gogly , DDS, MS, PhD. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006 ;101:E37- 44. di Summa PG et al., Adipose-derived stem cells enhance peripheral nerve regeneration, J Plast Reconstr Aesthet Surg (2009), doI: 10.1016 / j.bjps. 2009.0 9.012. Dohan DM. Safety issue associated with platelet-rich fibrin method. Oral Med Oral Radiol Endod 103: 587-592, 2007. Dohan DM, Rasmusson L, Alberktsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends in Biotechology 27: 158-167, 2009. Duailibi MT, Ashton BA, et al. Bioengineered teeth from cultured rat tooth bud cells. J Dent Res 83(7):523-8, 2004. Evans GRD. Peripheral nerve injury: A review and approach to tissue engineering constricts. The Anatomical Record 2001;263:396–404. Friedenstein AJ, Piatetzky S, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16: 381-90, 1966. Frisbie DD, Kisiday JD, Kawacak CE, Werpy NM, Wayne McIlwraith C. Evaluation of Adipose-Derived Stromal Vascular Fraction or Bone Marrow-Derived Mesenchymal Stem Cells for Treatment of Osteoarthritis. J Orthop Res 27:1675-1680, 2009. Gazitt Y, Reading CC, Hoffman R, Wickrema A, Vesole DH, Jagannath S, Condino J, Lee B, Barlogie B, Tricot G. Purified CD34+ Lin- Thy+ stem cells do not contain clonal myeloma cells. Blood 86, 381-389, 1995. Ghannam S, Bouffi C, Djouad F, Jorgensen C, Noel D. Immunosuppression by masenchymal stem cells:mechanisms and clinical applications. Stem cell Research&Therapy 1:2, 2010. Graziano A, d’Aquino R, Laino G, Papaccio G. Dental pulp stem cells: a promising tool for bone regeneration. Stem cells reviews. 2008;4:21-6. Gronthos S, Brahim J et al. Stem cell properties of human dental pulpstem cells. J dent Res. 2002;81(8):531-5. Gronthos S, Franklin DM, Leddy HA. Surface protein characterization of human adipose tissue-deriver stromal cell. JcellPhysiol 189:57-63, 2001. Gronthos S, Mankani M, et al., Postnatal human dental pulp stem cells (DPSCs) in vivo and in vitro. Proc Natl Acad Sci USA. 2000;97(25):13625-30. Goel, R.K et al. Effect of bone marrow-derived mononuclear cells on nerve regeneration in the transection model of the rat sciatic nerve. Journal of Clinical Neuroscience 16 (2009) 1211–1217 Hall S. Nerve repair: a neurobiologist’s view. J Hand Surg Br 2001;26:129–36. Haynesworth SE, Baber MA, Caplan AI. Cytokine expression by human-marrow derived mesenchymal progenitor cells in vitro:effects of dexamethason and IL-alpha. J Cell Physiol 166:585-592, 1999. Hu J, Zhu QT, Liu XL, et al. Repair of extended peripheral nerve lesions in rhesus monkeys using acellular allogenic nerve grafts implanted with autologous mesenchymal stem cells. Exp Neurol 2007;204:658e66. Hudson TW, Evans GRD, Schmidt CE. Engineering strategies for peripheral nerve repair. Clin Plast Surg 1999;26:617–628. Honda MJ, Ohara T, et al. Preliminary study of tissue-engineered odontogenesis in the canine jaw. J Oral Maxillofac Surg 64(2):283-9,2006. Honda MJ, Sumita Y, et al. Histological and immunohistochemical studies of tissue engineered odontogenesis. Arch Histol Cytol 68(2):89-101,2005. Ikeda M, Uemura T, Takamatsu K, Okada M, Kazuki K, Tabata Y, Ikada Y, Nakamura H. 2014. Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system. J Biomed Mater Res Part A 2014:102A:1370–1378. Javazon EH. Rat marrow stromal cells are more sensitive to plating density and 33 expand more rapidly from single-cell-derived colonies than marrow stromal cells. Stem cell 19:219-225, 2001. Keane, T.J. and S.F. Badylak. Biomaterials for tissue engineering applications. Semin Pediatr Surg 23(3):112-118. Kim BC, Bae H, Kwon IK, Lee EJ, Park JH, Khademhosseini A, et al. Osteoblastic/cementoblastic and neural differentiation of dental stem cells and their applications to tissue engineering and regenerative medicine. Tissue engineering Part B, Reviews. 2012;18:235-44. Kiyoshi Sakai, Akihito Yamamoto, et al. Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms. J Clin Invest. 2012;122(1):80–90. doi:10.1172/JCI59251. Kuo TF, Lin MF, Lin YH, Lin YC, Su RJ, Lin HW,Chan WP. Implantation of platele-rich fibrin and cartilage granules can facilitate cartilage repair in the injured rabbit knee:preliminary report. Clinics(Sao Paulo)66(10):1835-1838, 2011. Langer, R. and Vacanti . Tissue engineering. Science 260(5110):920-926, 1993. Lanza RP, Cibelli JB, West MD. Prospects for the use of nuclear transfer in human transplantation. Nature Biotechnology 17, 1171-1174, 1999. Laurens N, Koolwijk P, de Maat MP. Fibrin structure and wound healing. J Thromb Haemost 4: 932-939, 2006. Leitner GC, Gruber R, Neumuller J, Wagner A, Kloimstein P, Hocker P, Kormoczi GF, Buchta C. Platelet content and growth factor release in platelet-rich plasma: a comparison of four different system. Vox Sang 91: 135-139, 2006. Li, Hongmian. Et al. Autologous Platelet-Rich Plasma Promotes Neurogenic Differentiation of Human Adipose-Derived Stem Cells in vitro. International Journal of Neuroscience, 2013; 123(3): 184–190 LICHTENFELS, MARTINA. Et al. EFFECT OF PLATELET RICH PLASMA AND PLATELET RICH FIBRIN ON SCIATIC NERVE REGENERATION IN A RAT MODEL. Microsurgery 33:383–390, 2013. Levy, M., et al., Regenerative cellular therapies for neurologic diseases. Brain Research (2015). Lunquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrinogen factors in platelet-rich fibrin. Wound Rep Reg 16: 356-363, 2008. Majumdar MK, Thieda MA, Mosca JD, Moorman M, Gerson SL. Phenotype and functional comparison stem cells (MSC) and stromal cells. J Cell Physio 176:57-66, 1988. Mason, C. and P. Dunnill. A brief definition of regenerative medicine. Regen Med 3()1:1-5,2008. Meek MF, Coert JH. Clinical use of nerve conduits in peripheralnerve repair: Review of the literature. J Reconstr Microsurg 2002; 18:97–109. Metz, GA., Whishaw, IQ. Drug-induced rotation intensity in unilateral dopamine-depleted rats is not correlated with end point or qualitative measures of forelimb or hindlimb motor performance. Neuroscience 111, 325-36 (2002). Metz G.A., Whishaw I.Q. (2009). The Ladder Rung Walking Task: A Scoring System and its Practical Application.. JoVE. 28. http://www.jove.com/index/Details.stp?ID=1204, doi: 10.3791/1204 Miura M, Gronthos S, Zhoa M. SHED:stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U.S.A 100:5807-5812, 2003 Modino SA, and Sharpe PT. Tissue engineering of teeth usingadult stem cells. Arch Oral Biol 50(2:)255-8, 2005. Mosca JD. Mesenchymal stem cells as vehicles for gene therapy. Clin. Orthop Rel Res 379-S:71-90, 2000. Muraglia A, Cancedda R, Quarto R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J Cell Sci 113 ( Pt 7): 1161-6, 2000. Nakashima M, Reddi AH. The application of bone morphogenetic proteins to dental tissue engineering. Nat Biotechnol 21: 1025-1032, 2003. Nichols, C.M., T.M. Myckatyn, S. R. Rickman, K. Fox, T. Hadlock, and S.E. Mackinnon. Choosing the correct functional assay: A comprehensive assessment of functional test in the rat. Behav. Brain Res. 163: 143-158, 2005. Noble, J., C. A. Munro, V. S. Prasad, and R. Midha. Analysis of upper and lower extremity peripheral nerve injuries in a population of patients with multiple injuries. J. Trauma 45: 116-122, 1998. O’Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Rep Reg 16: 749-756, 2008. Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 410: 701-5, 2001. Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G, Becchetti E, Marchionni C, Alviano F, Fossati V, Staffolani N, Franchina M, Grossi A, Bagnara GP. Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation 80: 836-842, 2005. Pountos I, Giannoudis PV. Biology of mesenchymal stem cells. Care Injured 36 S, S8-S12, 2005. Richards EM, Baglin TP. Quantitation of reticulated platelets: methodology and clinical application. British Journal of Haematology Volume 91, Issue 2, pages 445–451, 1995. Rodrı ´guez FJ, Valero-Cabre ´ A, Navarro X. Regeneration and functional recovery following peripheral nerve injury. Drug Discovery Today: Disease Models 2004;1:177–185. Rohit Kumar Goel , Vaishali Suri . Effect of bone marrow-derived mononuclear cells on nerve regeneration in the transection model of the rat sciatic nerve. Journal of Clinical Neuroscience 16 (2009) 1211–1217 Ross MH, Pawlina W. Cartilage. In:Ross MH, ed. Histology, a text and atlas with correlated cell and molecular biology. 4th ed. USA, Lippincott Williams& Wilkins, 182-191, 2006. SCHAAKXS, DOMINIQUE et al. REGENERATIVE CELL INJECTION IN DENERVATED MUSCLE REDUCES ATROPHY AND ENHANCES RECOVERY FOLLOWING NERVE REPAIR. Muscle Nerve 47: 691–701, 2013 Stefano Geuna. The sciatic nerve injury model in pre-clinical research. Journal of Neuroscience Methods 243 (2015) 39–46. Snyder EY, Vescovi AL. The possibilities/perplexities of stem cells. Nature biotechnology 18, 827-828. 2000. Stamm C, Westphal B, Kleine HD, Petzsch M, Kittner C, Klinge H, Schumichen C, Nienaber CA, Freund M, Steinhoff G. Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 361: 45-6, 2003. Stevens A, Zuliani T, Olejnik C, LeRoy H, Obriot H, Kerr-Conte J, et al. Human dental pulp stem cells differentiate into neural crest-derived melanocytes and have label-retaining and sphere-forming abilities. Stem cells and development. 2008;17:1175-84. Stock UA, Vacanti JP. Tissue engineering:current state and prospects. Annu Rev Med 52:443-451, 2001. Su CY, Kuo YP, Nieh HL, Tseng YH, Burnouf T. Quantitative assessment of the kinetics of growth factors release from platelet gel. TRANSFUSION Volume 48: 2414-2420, 2008. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009. Sumita Y, Honda MJ, et al. Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering. Biomaterials 27(17):3238-48, 2006. Tsubasa Yamamoto, Yohei Osako, et al. Trophic Effects of Dental Pulp Stem Cells on Schwann Cells in Peripheral Nerve Regeneration. Cell Transplantation, 2015. Tzong-Fu Kuo et al , Implantation of platelet-rich fibrin and cartilage granules facilitates cartilage repair in the injured rabbit knee: preliminary report , CLINICS 2011;66(10):1835-1838. Ye, Fagang. Et al. Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury. Neural Regen Res. 2012 Oct 15; 7(29): 2286–2292. doi: 10.3969/j.issn.1673-5374.2012.29.007 Zumstein MA, Bielecki T, Dohan Ehrenfest DM. The Future of Platelet Concentrates in Sports Medicine: Platelet-Rich Plasma, Platelet-Rich Fibrin, and the Impact of Scaffolds and Cells on the Long-term Delivery of Growth Factors. Oper Tech Sports Med 19:190-197, 2011. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50268 | - |
dc.description.abstract | 坐骨神經的損傷對於患者來說不論是治療上或是社交上都會是一個沉重的經濟負擔,傳統的直接縫合以及移植治療並非適用於所有的患者。
近幾年,利用幹細胞進行組織再生的細胞性療法在全世界都是一個新興的研究,並且在許多的器官與組織的再生上都取得了相當不錯的成果,比如在治療膝蓋軟骨的損傷、急性腎衰竭、骨質疏鬆症、第一型的糖尿病上,在動物實驗中都已經證實具有治療的效果,此外在牙齒的再生上也已經得到非常顯著且樂觀的成果。 於此同時,由血液中經分離所得到的富含血小板纖維素(PRF),因為血小板內分泌顆粒含有數種生長因子,經過實驗證實其含有的生長因子對於組織修復和再生過程中,可幫助調節細胞的新陳代謝,以促進組織的修復和癒合。 針對大鼠坐骨神經的修復與再生,我們試驗了大鼠骨髓幹細胞分別及牙髓幹細胞各別混合了富含血小板纖維釋放液(PRFr)來進行治療。首先我們進行手術在大鼠的左後肢的坐骨神經上製造斷裂並在神經的兩端產生一段10mm寬的間隙,之後我們將實驗分組為1.)不做任何治療的對照組;2.)僅注射PRFr的治療組;3.)僅注射骨髓或牙髓幹細胞的治療組;4.)注射骨髓或牙髓幹細胞混合PRFr的治療組。注射的幹細胞量為1 x 106/0.1ml。注射治療後的三個月間每隔一個月進行大鼠的行走步伐測試,以藉此間接推測各組大鼠的坐骨神經在損傷後的恢復情形;注射治療後的三個月後犧牲大鼠取出左後肢的坐骨神經做肉眼及組織觀察及比較。結果顯示,不論是步伐測試的結果還是肉眼及組織切片的結果,三種治療方法均對不做任何治療的對照組有著顯著(p <0.05)的治療效果;而三種治療之間則是以幹細胞混合PRFr治療組的效果最好,其次為單獨幹細胞注射治療而PRFr的治療效果遜於另外兩者,而兩種幹細胞(骨髓幹細胞及牙髓幹細胞)之間彼此對於神經的再生效果並沒有顯著性的優劣(p >0.05),均是優於PRFr的治療但遜於幹細胞混合PRFr的治療。 | zh_TW |
dc.description.abstract | The injury of sciatic nerves was a serious problem to the patients, such as costing money for treatment and even worse to change their life. However, there were many limitations in the traditional therapy and as a result a better treatment should be found out. Recently, the cell-based therapy has been focused to repair the broken-down nerves. In the mean times, using Platelet-rich fibrin releasate (PRFr) to repair damaged organs was an insight in Regenerative Medicine.
Bone marrow derived stem cells (BMSCs) and Dental pulp derived stem cells (DPSCs) and PRFr were combined to treat with the animals. The 8 week old- injured nerve rats were separated into 6 groups. (1.) Control group, without any treatment; (2.) PRFr group,0.1 ml PRFr was injected; (3.) BMSCs group, 1x106 / 0.1 ml BMSCs were injected; (4.) DPSCs group, 1x106 / 0.1 ml DPSCs were injected; (5.) BMSC and PRFr group; (6.) DPSC and PRFr group, the rats were treated with 0.1 ml BMSCs and PRFr. Twelve weeks later, rats were sacrificed and the sciatic nerves regeneration was evaluated by gross and microscope. In gross, we measured the diameters of the regeneration sciatic nerves of every rats, then average every group and compared each other; in microscope, we calculate the axon density in the regeneration part of nerve fiber and average every group and compared. The result demonstrated that the combined treatment has significant impact than other groups. Following that was stem cells (BMSCs, DPSCs) treatment. However, the PRFr group was not efficiency as our expect but it still reached significant than the control group. In conclusion, cell based therapy may provide a new way to the nerve regeneration. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T12:34:28Z (GMT). No. of bitstreams: 1 ntu-105-R01644009-1.pdf: 5615912 bytes, checksum: fe75a302319eb97a778dfbd1f6951d16 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 致謝………………………………………………………………………I
目錄……………………………………………………………………II 圖目錄……………………………………………………………………V 表目錄…………………………………………………………………VII 附件……………………………………………………………………VIII 中文摘要…………………………………………………………………IX Abstracts………………………………………………………………XI 第一章 前言……………………………………………………………1 第二章 文獻探討………………………………………………………3 第一節 坐骨神經損傷的介紹…………………………………………3 第二節 血小板之介紹與應用…………………………………………4 2.1高濃度血小板血漿………………………………………………4 2.2富含血小板纖維素………………………………………………5 2.3富含血小板纖維素製作方式……………………………………6 2.4富含血小板纖維素釋放液………………………………………6 第三節 組織工程與再生醫學之介紹…………………………………7 3.1 組織工程…………………………………………………………7 3.2 再生醫學…………………………………………………………8 第四節 幹細胞之介紹與應用…………………………………………9 4.1 幹細胞之應用…………………………………………………9 4.2 間葉幹細胞之介紹 …………………………………………11 4.3 骨髓幹細胞之介紹及臨床應用 ……………………………12 4.4 牙髓幹細胞之介紹及臨床應用 ……………………………13 第三章 材料與方法……………………………………………………14 第一節 實驗材料製備 ………………………………………………14 1.1富含血小板纖維素釋放液的製備 ……………………………14 1.2間葉幹細胞的製備 ……………………………………………14 1.2.1骨髓幹細胞的製備 ………………………………………14 1.2.2牙髓幹細胞的製備 ………………………………………15 第二節 實驗動物 ……………………………………………………16 2.1 實驗動物的來源及飼養管理…………………………………16 2.2 實驗動物分組…………………………………………………17 第三節 實驗動物麻醉及手術 ………………………………………17 3.1 實驗動物麻醉方法及過程……………………………………17 3.2 實驗動物手術步驟……………………………………………17 第四節 手術後觀察 …………………………………………………18 4.1 水平橫梯行走測試……………………………………………18 第五節 犧牲實驗動物 ………………………………………………19 第六節 坐骨神經再生之評估 ……………………………………19 6.1 坐骨神經肉眼之評估 ………………………………………19 6.2 坐骨神經組織學之評估 ……………………………………19 第七節 統計與分析 ………………………………………………20 第四章 實驗結果………………………………………………………21 第一節 手術後的照護…………………………………………………21 第二節 水平橫梯行走測試之結果……………………………………21 第三節 坐骨神經在肉眼下再生之評估………………………………22 第四節 坐骨神經在組織學下再生之評估……………………………22 第五章 討論……………………………………………………………24 第六章 結論……………………………………………………………27 第七章 參考文獻………………………………………………………28 | |
dc.language.iso | zh-TW | |
dc.title | 應用骨髓幹細胞與牙髓幹細胞併合富含血小板纖維釋放液對坐骨神經缺損的修復與再生:以大鼠為模式 | zh_TW |
dc.title | Combination of Bone Marrow Derived Stem Cells and Dental Pulp Derived Stem Cells with Platelet-rich Fibrin Releasate (PRFr) to Repair and Regenerate Sciatic Nerves Injury | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張銘煌,許岩得,許世源 | |
dc.subject.keyword | 骨髓幹細胞,牙髓幹細胞,富含血小板纖維素,神經創傷,神經再生, | zh_TW |
dc.subject.keyword | Bone marrow derived stem cells,Dental pulp derived stem cells,Platelet-rich fibrin releasate,Nerve trauma,Nerve regeneration, | en |
dc.relation.page | 77 | |
dc.identifier.doi | 10.6342/NTU201601582 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-02 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 分子暨比較病理生物學研究所 | zh_TW |
顯示於系所單位: | 分子暨比較病理生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 5.48 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。