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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
dc.contributor.author | Chih-Zong Deng | en |
dc.contributor.author | 鄧志宗 | zh_TW |
dc.date.accessioned | 2021-06-15T12:31:34Z | - |
dc.date.available | 2021-08-24 | |
dc.date.copyright | 2016-08-24 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-03 | |
dc.identifier.citation | [1] Institute, N.C., Prostate-Specific Antigen (PSA) Test. [Online] Available: http://www.cancer.gov/types/prostate/psa-fact-sheet#q3.
[2] Glick, S., Rosalyn Sussman Yalow (1921-2011). Nature, Vol.(7353), No.(7353), pp. 580-580, 2011. [3] 范育睿, 利用微粒子追蹤測速儀量測C反應蛋白之布朗運動及其反應檢測. 碩士論文, 國立台灣大學,2008. [4] Burgi, D.S. and R.L. Chien, Optimization in sample stacking for high-performance capillary electrophoresis. Analytical chemistry, Vol.(18), No.(18), pp. 2042-2047, 1991. [5] Quirino, J.P. and S. Terabe, Exceeding 5000-fold concentration of dilute analytes in micellar electrokinetic chromatography. Science, Vol.(5388), No.(5388), pp. 465-468, 1998. [6] Robert S. Foote .,Julia Khandurina .,Stephen C. Jacobson ., and J. Michael Ramsey., Preconcentration of proteins on microfluidic devices using porous silica membranes. Analytical chemistry, Vol.(1), No.(1), pp. 57-63, 2005. [7] Quirino, J.P. and S. Terabe, Approaching a million-fold sensitivity increase in capillary electrophoresis with direct ultraviolet detection: cation-selective exhaustive injection and sweeping. Analytical chemistry, Vol.(5), No.(5), pp. 1023-1030, 2000. [8] Richard D. Oleschuk ., Loranelle L. Shultz-Lockyear ., Yuebin Ning ., and D. Jed Harrison., Trapping of bead-based reagents within microfluidic systems: on-chip solid-phase extraction and electrochromatography. Analytical chemistry, Vol.(3), No.(3), pp. 585-590, 2000. [9] Wang, Y.-C., A.L. Stevens, and J. Han, Million-fold preconcentration of proteins and peptides by nanofluidic filter. Analytical chemistry, Vol.(14), No.(14), pp. 4293-4299, 2005. [10] Qiaosheng Pu ., Jongsin Yun ., Henryk Temkin ., and Shaorong Liu., Ion-enrichment and ion-depletion effect of nanochannel structures. Nano letters, Vol.(6), No.(6), pp. 1099-1103, 2004. [11] Sung Jae Kim., Ying-Chih Wang., Jeong Hoon Lee., Hongchul Jang and Jongyoon Han1., Concentration polarization and nonlinear electrokinetic flow near a nanofluidic channel. Physical review letters, Vol.(4), No.(4), pp. 044501, 2007. [12] Lee, J.H., Y.-A. Song, and J. Han, Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane. Lab on a Chip, Vol.(4), No.(4), pp. 596-601, 2008. [13] Jeong Hoon Lee ., Yong-Ak Song ., Steven R. Tannenbaum ., and Jongyoon Han., Increase of reaction rate and sensitivity of low-abundance enzyme assay using micro/nanofluidic preconcentration chip. Analytical chemistry, Vol.(9), No.(9), pp. 3198-3204, 2008. [14] Nyborg, W.L., Acoustic streaming near a boundary. The Journal of the Acoustical Society of America, Vol.(4), No.(4), pp. 329-339, 1958. [15] Elder, S.A., Cavitation microstreaming. The Journal of the Acoustical Society of America, Vol.(1), No.(1), pp. 54-64, 1959. [16] Robin H. Liu ., Jianing Yang ., Maciej Z. Pindera , Mahesh Athavale and Piotr Grodzinski., Bubble-induced acoustic micromixing. Lab on a Chip, Vol.(3), No.(3), pp. 151-157, 2002. [17] Marmottant, P. and S. Hilgenfeldt, Controlled vesicle deformation and lysis by single oscillating bubbles. Nature, Vol.(6936), No.(6936), pp. 153-156, 2003. [18] Tovar, A.R. and A.P. Lee, Lateral cavity acoustic transducer. Lab on a Chip, Vol.(1), No.(1), pp. 41-43, 2009. [19] KrishnaáJuluri, B. and T. JunáHuang, A millisecond micromixer via single-bubble-based acoustic streaming. Lab on a Chip, Vol.(18), No.(18), pp. 2738-2741, 2009. [20] Daniel Ahmed., Hari S. Muddana., Mengqian Lu., Jarrod B. French., Adem Ozcelik., Ye Fang., Peter J. Butler.,Stephen J. Benkovic., Andreas Manz and Tony Jun Huang., Acoustofluidic chemical waveform generator and switch. Analytical chemistry, Vol.(23), No.(23), pp. 11803-11810, 2014. [21] Brenner, H., The slow motion of a sphere through a viscous fluid towards a plane surface. Chemical engineering science, Vol.(3-4), No.(3-4), pp. 242-251, 1961. [22] Goldman, A., R. Cox, and H. Brenner, Slow viscous motion of a sphere parallel to a plane wall—II Couette flow. Chemical engineering science, Vol.(4), No.(4), pp. 653-660, 1967. [23] Lin, B., J. Yu, and S.A. Rice, Direct measurements of constrained Brownian motion of an isolated sphere between two walls. Physical Review E, Vol.(3), No.(3), pp. 3909, 2000. [24] Lee, G.M., A. Ishihara, and K.A. Jacobson, Direct observation of Brownian motion of lipids in a membrane. Proceedings of the National Academy of Sciences, Vol.(14), No.(14), pp. 6274-6278, 1991. [25] Daniel Lavalette., Mark A. Hink., Martine Tourbez Catherine Te´treau and Antonie J. Visser., Proteins as micro viscosimeters: Brownian motion revisited. European Biophysics Journal, Vol.(6), No.(6), pp. 517-522, 2006. [26] K. D. Kihm., A. Banerjee., C. K. Choi and T. Takagi., Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM). Experiments in Fluids, Vol.(6), No.(6), pp. 811-824, 2004. [27] Yu Jui Fan., Horn Jiunn Sheen., Zheng Yu Chen., Yi Hsing Liu., Jing Fa Tsai., and Kuang Chong Wu.,TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum. Microfluidics and Nanofluidics, Vol.(1), No.(1), pp. 85-94, 2015. [28] Fan, Y.-J., et al., Detection of orchid viruses by analyzing Brownian diffusion of nanobeads and virus–immunobead association. Analytical Methods, Vol.(13), No.(13), pp. 5476-5482, 2015. [29] Melling, A., Tracer particles and seeding for particle image velocimetry. Measurement Science and Technology, Vol.(12), No.(12), pp. 1406, 1997. [30] Keane, R., R. Adrian, and Y. Zhang, Super-resolution particle imaging velocimetry. Measurement Science and Technology, Vol.(6), No.(6), pp. 754, 1995. [31] Raffel, M., C.E. Willert, and J. Kompenhans, Particle image velocimetry: a practical guide. Springer, 2013. [32] J. G. Santiago., S. T. Wereley., C. D. Meinhart., D. J. Beebe and R. J. Adrian., A particle image velocimetry system for microfluidics. Experiments in fluids, Vol.(4), No.(4), pp. 316-319, 1998. [33] R. sadr., M. yoda ., Z. zheng and A.T. conlisk., An experimental study of electro-osmotic flow in rectangular microchannels. Journal of fluid mechanics, Vol.(99), Issue(4-27), 357-367, 2004. [34] Prasad, A. and R.J. Adrian, Stereoscopic particle image velocimetry applied to liquid flows. Experiments in Fluids, Vol.(1), No.(1), pp. 49-60, 1993. [35] Willert, C., Stereoscopic digital particle image velocimetry for application in wind tunnel flows. Measurement science and technology, Vol.(12), No.(12), pp. 1465, 1997. [36] Abhay Vishnoi., Amit Saxena and Aman Bhatia., Biochip-a review on cutting-edge biochip technology. International Journal of Scientific Research and Management Studies, Vol.(2) Issue(10), 405-411 [37] Terry, S.C., J.H. Jerman, and J.B. Angell, A gas chromatographic air analyzer fabricated on a silicon wafer. Electron Devices, IEEE Transactions on, Vol.(12), No.(12), pp. 1880-1886, 1979. [38] Manz, A., N. Graber, and H.á. Widmer, Miniaturized total chemical analysis systems: a novel concept for chemical sensing. Sensors and actuators B: Chemical, Vol.(1), No.(1), pp. 244-248, 1990. [39] Xia, Y. and G.M. Whitesides, Soft lithography. Annual review of materials science, Vol.(1), No.(1), pp. 153-184, 1998. [40] McDonald, J.C. and G.M. Whitesides, Poly (dimethylsiloxane) as a material for fabricating microfluidic devices. Accounts of chemical research, Vol.(7), No.(7), pp. 491-499, 2002. [41] Gongadze, E., U. Rienen, and A. Iglič, Generalized stern models of the electric double layer considering the spatial variation of permittvity and finite size of ions in saturation regime. Cellular and Molecular Biology Letters, Vol.(4), No.(4), pp. 576-594, 2011. [42] 鍾珮珊, 利用電流特性觀察微奈米濃縮晶片之作用機制及其應用. 碩士論文,國立台灣大學, 2013. [43] Young, T., An essay on the cohesion of fluids. Philosophical Transactions of the Royal Society of London, Vol.(95), pp. 65-87, 1805. [44] Wang, Shutao, and Lei Jiang. 'Definition of superhydrophobic states.'Advanced Materials 19.21 (2007): 3423-3424.. [45] Leighton, T., Acoustic bubble detection-I. The detection of stable gas bodies. Environmental engineering, Vol.(7), pp. 9-16, 1994. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50172 | - |
dc.description.abstract | 本研究成功開發了一種新型檢測方法,整合了預濃縮機制(preconcentration mechanism)、聲學激發震盪的氣泡閥門(acoustically excited oscillating-bubble valve)、以及布朗運動檢測(Brownian motion detection)。首先,利用微影製程技術,製作出微米級流道,再將此兩條流道中間,舖以多孔性奈米材料Nafion,作為離子選擇性薄膜(ion-selective membrane),其中微米級流道上設計有兩對凹槽,當流體通入微流道時,因為表面張力與微流道本身材料之疏水特性,微型氣泡將會生成於凹槽中。
本研究之生物樣本為C反應蛋白(C-reactive protein, CRP),是一種研究上常使用的非特異性的反應蛋白。實驗時,先將修飾於335 nm螢光奈米粒子(fluorescent sphere)的anti-CRP與不同濃度的CRP混合後,通入微流道中,再施加電位差於Nafion的兩端,以產生濃度極化效應(Ion Concentration Polarization, ICP),此時一端為離子富集區(ion enrichment region),另一端為離子空乏區(ion depletion region),接者在空乏區一側施加偏壓,便可利用第二種電滲流 (electroosmosis of the second kind),使空乏區之高電壓側產生濃縮區塊(preconcentration plug),此時因為流道的特殊設計,濃縮區塊會穩定的停滯在流道特定位置的腔體內。濃縮區塊產生後,啟動事前黏附在晶片上的壓電片(piezoelectric transducer),調整至適當的振幅及特定的頻率後,於流道凹槽裡位於濃縮區塊兩側的氣泡對,將因為壓電片震動產生特定的共振頻率,而快速膨脹,直到可以完全阻擋流道,進而捕捉濃縮區塊。之後,由於無電位差及壓力差,流體呈現靜止狀態,此時以粒子追蹤測速儀(Particle Tracking Velocimetry, PTV)量測螢光粒子的布朗運動速度,並進行分析。當anti-CRP因與CRP有專一性而互相鍵結,螢光粒子的布朗運動速度會因為粒子變大而減緩,而布朗運動速度降低的程度可作為待測樣本濃度高低的依據。 此種感測技術可廣泛應用在蛋白質或是病毒感測,可以達到快速、價格低廉、且可及時(real-time)感測。此研究成功地可以量測低濃度之CRP,再利用預濃縮機制,可達到極低偵測極限(0.105 ng/ml)的效果。 | zh_TW |
dc.description.abstract | In this study, we demonstrate a new integrated detection method to achieve ultra-low detection limit and high sensitivity. This technique used via electrokinetic trapping (EKT)-based nanofluidic preconcentration mechanism, Brownian diffusion and oscillating trapped bubbles. The geometry-trapped bubbles can be triggered to oscillate at resonance frequency by acoustic wave. This acoustically excited oscillating bubble valves haves several advantages: (1) simple fabrication (one layer compareds to multiple-layer of pneumatic valve), (2) perfect channel-blocking channel perfectly (without leakage problem), (3) multiple valves which can be selectively turn off easily. For immunoassay, the oscillated bubbles grew rapidly to block the microchannel and led to trap preconcentrated antigen plug and antibody-coated nanobeads. The antigen concentration can be quantitatively analyzed by real-time measurement of the immunobeads Brownian diffusion. The test sample is C-reactive protein (CRP) which), CRP is a risk indicator of coronary heart disease and atherosclerosis. The level of CRP increases also response to inflammation, infection and tumor etc. This biomarker is widely used to predict coronary events
In conclusions, we developed an integrated biosensor that can haveachieve sample preconcentrating, sample collecting, and sample concentration sensing. The concentrating factor can reach up to 〖~10〗^6 〖~10〗^6 and the sample plug can be trapped by bubble valves after collecting. Thus, an ultra-sensitive (as low as 0.105 ng/ml) and fast (10 minutes) immunoassay can be achieved. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T12:31:34Z (GMT). No. of bitstreams: 1 ntu-105-R03543028-1.pdf: 3796426 bytes, checksum: 7ac378fb2444ac7e9a1e96f2b81ea607 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 摘要 ii ABSTRACT iii 目錄 iv 第一章 導論 1 1.1 前言 1 1.2 研究動機 2 1.3 研究方法 2 1.4 論文架構 3 第二章 文獻回顧 4 2.1 生物檢測技術 4 2.1.1 免疫分析法(Immunoassay) 4 2.1.2 生物感測 4 2.2 預濃縮技術 5 2.2.1 預濃縮技術發展背景 5 2.2.2 奈米流體預濃縮技術 5 2.3 聲學震盪氣泡 8 2.4 布朗運動 12 2.5 粒子影像測速儀/粒子追蹤測速儀 14 2.6 生物晶片 15 第三章 實驗原理 17 3.1 生物感測基本工作原理 17 3.1.1 生物分子辨識 17 3.1.2 抗體與抗原之特異性鍵結 17 3.2 電驅動微奈米濃縮晶片之工作原理 18 3.2.1 電雙層 18 3.2.2 離子的區域性空乏與濃縮現象 20 3.2.3 預濃縮機制(Mechanism of preconcentration) 22 3.3 震盪氣泡閥門 24 3.3.1 表面張力 24 3.3.2 聲學激發震盪氣泡閥門工作原理 25 3.4 布朗運動 25 3.4.1 愛因斯坦關係式 26 3.4.2 朗之文方程式 28 3.5 粒子影像/追蹤測速技術工作原理 30 第四章 實驗設備架構與實驗方法 32 4.1 氣泡閥門奈米流體預濃縮晶片設計與製程 32 4.1.1 流道設計與製程 33 4.1.2 塗布離子選擇性薄膜 36 4.1.3 氧電漿接合製程 37 4.1.4 貼附壓電片 38 4.2 螢光粒子表面之抗體修飾 39 4.2.1 C反應蛋白(C-reactive protein, CRP)簡介 39 4.2.2 螢光粒子 39 4.2.3 抗體修飾之材料與設備 抗體修飾材料如下: 40 4.2.4 抗體體修飾流程 41 4.2.5 抗體用量估算方式 41 4.3 量測與觀測系統實驗設備 42 4.3.1 觀測系統 42 4.3.2 量測系統 43 4.4 其他實驗設備與軟體 45 第五章 實驗結果與討論 47 5.1 聲學震動氣泡閥門驗證 47 5.2 預濃縮系統驗證 53 5.3 布朗運動量測結果 55 5.3.1 相對高濃度C反應蛋白布朗運動量測 55 5.3.2 預濃縮處理極低濃度C反應蛋白布朗運動檢測 57 第六章 總結與未來展望 61 6.1 結論 61 6.2 未來展望 62 | |
dc.language.iso | zh-TW | |
dc.title | 整合氣泡閥門與奈米流道預濃縮器的生物標記物檢測方法 | zh_TW |
dc.title | A new biomarker detection method by integration of oscillating-bubble valves with nanofluidic preconcentrator | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 田維誠(Wei-Cheng Tian),呂家榮(Chia-Jung Lu) | |
dc.subject.keyword | 布朗運動,氣泡閥門,粒子追蹤測速儀,C反應蛋白,生物感測技術, | zh_TW |
dc.subject.keyword | preconcentrator,oscillating microbubble,nanofluidic,immunoassay,C-reactive protein,Brownian mtion,micro-Particle Tracking Velocimetry (μ-PTV), | en |
dc.relation.page | 65 | |
dc.identifier.doi | 10.6342/NTU201601817 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-04 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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