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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 賴明宗(Ming-Zong Lai) | |
| dc.contributor.author | Cheng-Hsun Jao | en |
| dc.contributor.author | 饒承訓 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:43:11Z | - |
| dc.date.available | 2021-08-26 | |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-15 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49706 | - |
| dc.description.abstract | 半乳凝集素-3是一種β-半乳糖苷結合凝集素，它對於各種生物功能，例如細胞-細胞相互作用、細胞週期調控、細胞生長、發炎反應和腫瘤發展進程中扮演重要角色。此外，研究也顯示，半乳糖凝集素-3亦可調節各種傳染病和某些特定類型的癌症。然而，半乳凝集素-3在發炎反應中所扮演的角色仍未被釐清。我們使用了骨髓源巨噬細胞來進行研究，探討半乳凝集素-3在脂多醣所誘發的發炎反應中所扮演的角色。結果發現，發炎性細胞激素和發炎小體的前驅物的表現在半乳凝集素-3缺乏的巨噬細胞中有顯著下降的情形。進一步也發現類鐸受體-4下游的信號也較弱。然而，在我們的動物模型所做出來的結果卻認為半乳凝集素-3是一個負向調控者，這個結果暗示著，半乳凝集素-3在不同亞型的巨噬細胞中可能扮演著不同的角色。從我們的實驗結果可以歸納出，內源性半乳凝集素-3在不同類型的巨噬細胞中可能有著不同的功能。未來我們會繼續著重在不同亞型的巨噬細胞，進一步去釐清半乳凝集素-3在發炎小體活化中所扮演的角色，讓我們能更清楚地知道半乳凝集素-3具有怎樣的功能。 | zh_TW |
| dc.description.abstract | Galectin-3 is a beta-galactoside-binding lectin that participates in orchestrating various biological functions such as cell-cell interaction, cell cycle control, cell growth, inflammation and tumor progression. Also, studies have shown that galectin-3 is involved in the regulation of various infectious diseases and certain type of cancer. Nevertheless, a number of issues related to the role of galectin-3 in inflammation remain to be addressed. Here, we investigated the role of galectin-3 in inflammatory responses triggered by LPS treatment within bone marrow-derived macrophages. As a result, we found that proinflammatory cytokines and precursors of inflammasomes were significantly downregulated in galecitn-3-deficient macrophages. Moreover, TLR4 downstream signaling was impaired. However, our in vivo assay showed that galectin-3 might be a negative regulator. These results imply that galectin-3 might have different functions in different subtypes of macrophage. In summary, our results suggest that endogenous galectin-3 might play distinct roles in various macrophage subtypes. Additional studies are required to elucidate how endogenous galecitn-3 regulates inflammasome activation in different subtypes of macrophages, which it may also help us understand its role in innate immune system. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:43:11Z (GMT). No. of bitstreams: 1 ntu-105-R03449012-1.pdf: 1387201 bytes, checksum: ae8230beb2071b7fa1506a466b43aa90 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | Abstract I
摘要 II Introduction 1 1. Macrophages – role of macrophages in homeostasis 1 2. Innate immunity 2 2-1. Pattern-recognition receptors (PRRs) 2 2-2. NLRP3 Inflammasomes 3 3. Galectins 7 3-1. Galectin family (β-galactoside binding proteins) 7 3-2. Galectin-3 biology 8 3-3. The function of galectin-3 8 3-4. Crosstalk between galectin-3 and NLRP3 inflammasome 9 Materials and Methods 12 1. Reagents. 12 2. Mice. 12 3. The generation of bone marrow-derived macrophages and ell culture. 13 4. Protein analysis by western blot and ELISA. 13 5. Immunoprecipitation. 15 6. Quantitative PCR. 15 7. Flow cytometry. 16 8. Monosodium urate (MSU)-mediated peritonitis. 16 9. Immunofluorescence assay. 17 10. Statistical analysis. 18 Result 19 1. Comparable macrophage surface marker expression between WT and Gal3-/- BMDMs 19 2. Reduced gene expression levels of TNFα, IL-1β and NLRP3 in Gal3-/- BMDMs. 19 3. Reduced levels of proinflammatory cytokines in Gal3-/- BMDMs. 20 4. Galectin-3 deficiency impairs the level of precursors in NLRP3 inflammasome. 20 5. Galectin-3 is required for TLR4 signaling. 21 6. Interaction of galectin-3 with NLRP3, procaspase-1 and ASC. 22 7. Enhanced MSU-induced peritonitis in Gal3-deficient peritoneal macrophages 22 Discussion 24 Reference 27 Figure contents 36 Figure 1. Comparable macrophage surface marker expression between WT and Gal3-/- BMDMs. 36 Figure 2. Reduced gene expression levels of TNFα, IL-1β and NLRP3 in Gal3-/- BMDMs. 37 Figure 3. Reduced cytokine secretion in Gal3-/- BMDMs. 38 Figure 4. Deficiency of galectin-3 impairs the level of NLRP3 and proIL-1β, leading to reduced IL-1β production. 39 Figure 5. Galectin-3 is required for the NFκB pathway. 40 Figure 7. Increased cytokine secretion in Gal3-/- BMDMs. 42 Figure 8. Interaction between galectin-3 and NLRP3. 43 Appendix - 1 44 Appendix – 2 48 | |
| dc.language.iso | en | |
| dc.subject | 巨噬細胞 | zh_TW |
| dc.subject | 半乳糖凝集素-3 | zh_TW |
| dc.subject | NLRP3 發炎小體 | zh_TW |
| dc.subject | 類鐸受體-4 | zh_TW |
| dc.subject | Galectin-3 | en |
| dc.subject | TLR4. macrophages | en |
| dc.subject | NLRP3 inflammasome | en |
| dc.title | 半乳糖凝集素-3在介白素-1β的表現中所扮演的角色 | zh_TW |
| dc.title | The role of Galectin-3 in IL-1β production. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 劉扶東(Fu-Tong Liu) | |
| dc.contributor.oralexamcommittee | 伍安怡(Betty Wu-Hsieh) | |
| dc.subject.keyword | 半乳糖凝集素-3,NLRP3 發炎小體,類鐸受體-4,巨噬細胞, | zh_TW |
| dc.subject.keyword | Galectin-3,NLRP3 inflammasome,TLR4. macrophages, | en |
| dc.relation.page | 48 | |
| dc.identifier.doi | 10.6342/NTU201602665 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-15 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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