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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張嘉銓 | |
| dc.contributor.author | Jhih-Jhong Wang | en |
| dc.contributor.author | 王致中 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:34:13Z | - |
| dc.date.available | 2021-08-26 | |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-16 | |
| dc.identifier.citation | 1. 新編中藥大辭典(1985). 上卷, 出版社：新文豐出版公司, 663–667頁.
2. Huang, T.-C.; Hsieh, C.-F.; Boufford, D.E.; Kuoh, C.-S.; Ohashi, H.; Peng, C.-I.; Tsai, J.-L.; Yang, K.-C.; Hsiao, A.; Tsai, J.-M. Flora of Taiwan, 2nd ed.; National Science Council, R.O.C., 1984; Vol. 6; pp 845–847. 3. Wen, C.-L. Dendrobium Cassiope. R.O.C. patent 980,092, Sep 16, 2009. 4. Luo, A.; He, X.; Zhou, S.; Fan, Y.; He, T.; Chun, Z. In vitro antioxidant activities of a water-soluble polysaccharide derived from Dendrobium nobile Lindl. Extracts. Int. J. Biol. Macromol. 2009, 45, 359–363. 5. Luo, A.; He, X.; Zhou, S.; Fan, Y.; Luo, A.; Chun, Z. Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl. Carbohydr. Polym. 2010, 79, 1014–1019. 6. Luo, A. Immune stimulating activity of water-soluble polysaccharide fractions from Dendrobium nobile Lindl. Afr. J. Pharm. Pharmacol. 2011, 5, 625–631. 7. Xu, J.; Li, S.-L.; Yue, R.-Q.; Ko, C.-H.; Hu, J.-M.; Liu, J.; Ho, H.-M.; Yi, T.; Zhao, Z.-Z.; Zhou, J.; Leung, P.-C.; Chen, H.-B.; Han, Q.-B. A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study. Anal. Bioanal. Chem. 2014, 406, 6409–6417. 8. Meng, L.-Z.; Lv, G.-P.; Hu, D.-J.; Cheong, K.-L.; Xie, J.; Zhao, J.; Li, S.-P. Effects of polysaccharides from different species of Dendrobium (Shihu) on macrophage function. Molecules 2013, 18, 5779–5791. 9. Xing, X.; Cui, S. W.; Nie, S.; Phillips, G. O.; Goff, H. D.; Wang, Q. Study on Dendrobium officinale O-acetyl-glucomannan: part II. fine structures of O-acetylated residues. Carbohydr. Polym. 2015, 117, 422–433. 10. Li, Q.; Xie, Y.; Su, J.; Ye, Q.; Jia, Z. Isolation and structural characterization of a neutral polysaccharide from the stems of Dendrobium densiflorum. Int. J. Biol. Macromol. 2012, 50, 1207–1211. 11. Hsieh, Y. S.-Y.; Chien, C.; Liao, S. K.-S.; Liao, S.-F.; Hung, W.-T.; Yang, W.-B.; Lin, C.-C.; Cheng, T.-J. R.; Chang, C.-C.; Fang, J.-M.; Wong, C.-H. Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense. Bioorg. Med. Chem. 2008, 16, 6054–6068. 12. Shi, L. Bioactivities, isolation and purification methods of polysaccharides from natural products: a review. Int. J. Biol. Macromol. 2016, 92, 37–48. 13. Johnson Jr., C. S. Diffusion ordered nuclear magnetic resonance spectroscopy: principles and applications. Prog. Nucl. Magn. Reson. Spectrosc. 1999, 34, 203–256. 14. Groves, P.; Rasmussen, M. O.; Molero, M. D.; Samain, E.; Cañada, F. J.; Driguez, H.; Jiménez-Barbero, J. Diffusion ordered spectroscopy as a complement to size exclusion chromatography in oligosaccharide analysis. Glycobiology 2004, 14, 451–456. 15. Agrawal, P. K. NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides. Phytochemistry 1992, 31, 3307–3330. 16. Human Metabolome Database: 1H NMR Spectrum (HMDB00258) http://www.hmdb.ca/spectra/nmr_one_d/1293 (accessed Jun 6, 2016). 17. Human Metabolome Database: 13C NMR Spectrum (HMDB00258) http://www.hmdb.ca/spectra/nmr_one_d/1220 (accessed Jun 6, 2016). 18. Shrestha, S.; Lee, D.-Y.; Park, J.-H.; Cho, J.-G.; Lee, D.-S.; Li, B.; Kim, Y.-C.; Kim, G.-S.; Bang, M.-H.; Baek, N.-I. Phenolic components from Rhus parviflora fruits and their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages. Nat. Prod. Res. 2013, 27, 2244–2247. 19. Cheng, J.-J.; Lin, C.-Y.; Lur, H.-S.; Chen, H.-P.; Lu, M.-K. Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola. Process Biochem. 2008, 43, 829–834. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49549 | - |
| dc.description.abstract | 金奮石斛 (Dendrobium Cassiope, 金童1號) 為金釵石斛 (Dendrobium nobile, ♀) 及奮起湖銅皮石斛 (D. moniliforme, ♂) 之雜交種，是一種蘭科石斛屬藥用植物，人工培養於臺灣。石斛是使用數千年的中草藥，具有多種生物活性，例如：抗糖尿病、預防心血管疾病、免疫調節等。石斛內含豐富的多醣類；然而，以層析法純化及分析多醣的方法相當地複雜及耗費時間，因此開發簡化的純化方法是必需的。本研究利用離心過濾法自金奮石斛水提取物中快速分離得到分子量大於3 kDa，介於3 kDa及1 kDa，以及小於1 kDa共三個部分。分子量3 kDa以上部分經由二乙胺基乙基纖維素管柱層析、核磁共振技術、單醣組成分析，主要成分DC-PS1為葡萄甘露聚醣(glucomannan)，甘露糖：葡萄糖之組成比例為100 : 12.5，並具有部分2-O及3-O乙醯基取代。其主幹為1,4鍵結的β-甘露糖和1,4鍵結的β-葡萄糖，另有1,6-α-甘露糖分支及β-甘露糖之終端糖基。經由高效分子篩層析及擴散排序核磁共振波譜分析其分子量為3.308 kDa，聚合度為20。分子量1 kDa至3 kDa部分則得到sucrose及shikimic acid，其中α-葡萄糖及β-果糖之比例為5 : 3。此部分不如預期得到寡醣成分，顯示離心過濾法對於分子量在1 kDa區間之分離效果不理想。 | zh_TW |
| dc.description.abstract | Dendrobium Cassiope (Taiseed Golden Boy No.1), a hybrid of Dendrobium nobile (♀) and D. moniliforme (♂), is a medicinal dendrobium (Shi Hu; Orchidaceae) cultivated in Taiwan. Dendrobium has been used as a traditional Chinese medicine for thousands of years. It shows several bioactivities, such as anti-hyperglycemic, beneficial for cardiovascular diseases, and immunity modulation. Dendrobium is abundant in polysaccharides (PS). Chromatographic purification and analyses of PS are complicated and time-consuming. Therefore, development of strategies with simplified procedures for PS are necessary. The present study utilized centrifugal filtration for rapid purification of the PS with different molecular weight (>3 kDa, 1 kDa-3kDa, and < 1 kDa) from the aqueous extract of Dendrobium Cassiope. The PSs with a molecular weight over 3 kDa and between 1 kDa to 3 kDa were purified by DEAE-cellulose column chromatography and characterized by nuclear magnetic resonance spectroscopy (NMR) and monosaccharides composition analysis. The purified PSs (DC-PS1) with a molecular weight over 3 kDa are acetyl glucomannans, with a backbone of β-(1→4)-mannose and β-(1→4)-glucose units in a ratio of 100:12.5, and with 1,6-α-mannose branches and β-mannose terminals. The molecular weight of purified PSs were estimated to be 3.308 kDa [a degree of polymerization (DP) of 20] by high performance size exclusion chromatography (HPSEC) and diffusion-ordered NMR (DOSY). The fraction with a molecular weight between 1 kDa to 3 kDa has sucrose and shikimic acid with α-glucose and β-fructose in a ratio of 5:3. The fraction does not contain oligosaccharides, thus the purifying efficacy of centrifugal filtration with molecular weight around 1 kDa is not favorable. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:34:13Z (GMT). No. of bitstreams: 1 ntu-105-R03423021-1.pdf: 5916535 bytes, checksum: 1a5b822d0ec914e856ec81eaa8ad3ed2 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 1 緒論與研究目的 1
1.1 植物簡介 1 1.2 石斛屬葡萄甘露聚醣類之相關研究 2 1.3 多醣的純化方法回顧 4 1.3.1 分級沉澱法 (Graded precipitation) 4 1.3.2 鹽析法 (Salting-out method) 4 1.3.3 金屬配位法 (Metal coordination) 4 1.3.4 膠體過濾法 (Gel filtration) 5 1.3.5 陰離子交換層析法 (Anion exchange chromatography) 5 1.4 離心過濾法 (Centrifugal filtration) 6 1.5 擴散排序核磁共振譜 (Diffusion-Ordered NMR Spectroscopy, DOSY) 7 1.6 研究目的 9 2 實驗結果與討論 10 2.1 葡萄甘露聚醣DC-PS1之化學結構解析 11 2.1.1 單醣組成分析 11 2.1.2 一維及二維核磁共振波譜解析 13 2.1.2.1 核磁共振氫譜 (1H NMR) 圖譜解析 13 2.1.2.2 核磁共振碳譜 (13C NMR) 及DEPT-135圖譜解析 14 2.1.2.3 COSY圖譜解析 15 2.1.2.4 NOESY圖譜解析 17 2.1.2.5 TOCSY圖譜解析 19 2.1.2.6 HSQC圖譜解析 21 2.1.2.7 HMBC圖譜解析 25 2.1.3 單醣單元之α, β組態之辨別 34 2.1.4 分子量測定 35 2.1.4.1 擴散排序核磁共振波譜(Diffusion-Ordered NMR Spectroscopy, DOSY) 35 2.1.4.2 高效分子篩層析色譜 (High performance size exclusion chromatogram, HPSEC) 38 2.1.5 多醣結構之討論 39 2.2 Fr. 1KU之化合物研究 40 2.2.1 Fr. 1KU之單醣組成分析 40 2.2.2 Fr. 1KU之NMR解析 41 2.2.3 Fr. 1KU之甲基化反應及所含化學成分結構推判 43 2.2.4 Fr. 1KU實驗結果之討論 45 2.3 結論 46 3 實驗部分 47 3.1 儀器與器材 47 3.1.1 理化性質測定儀器 47 3.1.2 成分分離及分析之儀器及材料 47 3.1.3 試劑與溶劑 48 3.2 金奮石斛 (Dendrobium Cassiope) 化學成分之分離 49 3.2.1 金奮石斛水提取物來源 49 3.2.2 葡萄甘露聚醣之純化 49 3.2.3 酚－硫酸測試 49 3.2.4 酸水解反應 49 3.2.5 單醣分析 50 3.2.6 甲基化反應 50 3.2.7 HPSEC分子量標準曲線之建立 51 參考文獻 52 附圖 54 | |
| dc.language.iso | zh-TW | |
| dc.subject | 多醣 | zh_TW |
| dc.subject | 石斛 | zh_TW |
| dc.subject | 金奮石斛 | zh_TW |
| dc.subject | Dendrobium Cassiope | en |
| dc.subject | Dendrobium | en |
| dc.subject | Saccharides | en |
| dc.title | 金奮石斛醣類化學成分之研究 | zh_TW |
| dc.title | Chemical Investigation of Saccharides of Dendrobium Cassiope | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李水盛,盧美光 | |
| dc.subject.keyword | 石斛,金奮石斛,多醣, | zh_TW |
| dc.subject.keyword | Dendrobium,Dendrobium Cassiope,Saccharides, | en |
| dc.relation.page | 71 | |
| dc.identifier.doi | 10.6342/NTU201602947 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-17 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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