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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49491完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 柯淳涵(Chun-Han Ko) | |
| dc.contributor.author | Yen-Ting Liu | en |
| dc.contributor.author | 劉彥廷 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:31:14Z | - |
| dc.date.available | 2021-08-30 | |
| dc.date.copyright | 2016-08-30 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-16 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49491 | - |
| dc.description.abstract | 造紙行業是個不可缺少的重要產業。而在造紙過程中，所生產出來之產品均經過打漿處理，而此處理方式會增加纖維的帚化及接合能力。第二代生質燃料是受到高度重視的新能源，而其透過水解木質纖維素，作為生產生質燃料的主要來源。將酵素加入紙漿中進行一段時間的處理後，纖維素酶添加量對紙漿所產生之影響也有進一步的討論，隨著酵素劑量的增加，其所得到的還原糖產量也隨之上升。然而酵素劑量與其酵素水解效率並非成正比關係。隨著使用不同的酵素劑量進行水解，其酵素水解效率也不盡相同。過高及過低的酵素劑量不能使酵素水解效率達到最好的狀況，需要適當的酵素劑量才能達到最好的水解效率。
而在水解實驗方面，較多的全纖維素可以有較高的可及性及反應性，此結果可以由轉變出來之還原糖量所得知。較高的結晶度(CI)會降低酵素對纖維的可及性，而木質素則會在酵素水解纖維素時產生阻礙。增加反應表面積也可以增加水解效率，但還原糖變化沒有隨著纖維尺寸改變而有明顯的變化。 本研究藉由不同前處理、酵素添加量及打將能量消耗，來探討對不同生質物所產生之影響。而不同生質物的特性與酵素水解後所產生之還原糖產量之關聯也在本研究中進行進一步的探討。 | zh_TW |
| dc.description.abstract | Paper industry is an important and indispensable industry. During paper making process, the production fibers has to be refined, and this mechanically treated has present its bonding potential. Refining is a highly energy consuming stage, therefore, various treated of cellulase to fibers as a pretreatment were investigated to reduce the energy consumption and improve paper properties. The enzyme wat added into pulp and treated for a couple of time. The impact of cellulase loading that added to the pulp was identified, with the enzyme loading increasing, the conversion of sugars is increased. However, enzyme loading is not proportional to the enzyme efficiency. With different dosages for the hydrolysis, different enzyme efficiency was measured. Both excessive and low loading of cellulase could not achieve optimized enzyme efficiency, and moderate loading of cellulase could have achieved.
In the aspect of hydrolysis, more holocellulose have more accessibility and reactivity which are estimated by conversion of sugars. Higher crystallinity index (CI) decreased the accessibility of the enzyme, and lignin was also an obstacle that obstruct the enzyme hydrolysis with cellulose. The surface area can increase the efficiency, but the conversion has no significant different from the fiber sizes. This study investigated different properties of biomass by different pretreatments, enzyme loading and the refined energy demand. Correlation between properties of different biomass and glucose yield after enzyme hydrolysis were also researched. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:31:14Z (GMT). No. of bitstreams: 1 ntu-105-R03625042-1.pdf: 2477341 bytes, checksum: 01898701d996d994efb0c38375f450af (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 謝誌 i
摘要 ii Abstract iii Abbreviation iv Contents vi Figures Index viii Tables Index x Chapter 1 Introduction 1 Chapter 2 Literature Review 4 2.1 Structure of Lignocellulose 4 2.1.1 Cellulose 4 2.1.2 Hemicellulose 5 2.1.3 Lignin 5 2.2 Enzymatic Hydrolysis 6 2.3 Refining 8 2.3.1 Hollander Beater 9 2.3.2 PFI Mill 10 2.3.3 Specific Edge Load Theory 11 2.4 Catalytic Domains and Carbohydrate Binding Modules 12 2.5 Pretreatment 13 2.5.1 Fiber Size 15 2.5.2 Steam Explosion 16 2.5.3 Delignification 18 Chapter 3 Materials and Method 20 3.1 Materials 20 3.1.1 Substrates 20 3.1.2 Enzyme 21 3.2 Experiment and Analytical Methods 22 3.2.1 Materials Sieved 22 3.2.2 PFI Refining 22 3.2.3 Hollander Beater Refining 23 3.2.4 CSF Test 24 3.2.5 Enzyme Hydrolysis 24 3.2.6 Reducing Sugar Determination 25 3.2.7 Fiber Morphology 25 3.2.8 X-ray Diffraction Measurements 26 Chapter 4 Result and Discussion 27 4.1 Properties of Lignocellulose 27 4.1.1 Chemical Composition of Pulps 27 4.1.2 Crystallinity Indexes 28 4.2 The Effect of Different Substrate 30 4.2.1 The Reducing Sugar on Hydrolysis 30 4.2.2 The Enzyme Efficiency 31 4.2.3 The effect on the Hollander beater pretreatment 33 4.2.4 Effects on PFI Pretreatment 35 4.2.5 Crystallinity Indexes 36 4.2.6 Fiber Morphology 38 4.3 The Effect of Different Enzyme Loading 42 4.3.1 The Reducing Sugar on Hydrolysis 42 4.3.2 The Enzyme Efficiency 46 4.3.3 The degree of Crystallinity 50 4.3.4 Fiber Morphology 52 4.4 Effects of Different Pretreatments 57 4.4.1 The Pretreatment Effect on Hydrolysis 58 4.4.2 Effects on Refining Pretreatment 63 4.4.3 Fiber Morphology 67 Chapter 5 Conclusion 75 Reference 77 | |
| dc.language.iso | en | |
| dc.subject | 結晶度 | zh_TW |
| dc.subject | 可及性 | zh_TW |
| dc.subject | 打漿 | zh_TW |
| dc.subject | 酵素水解 | zh_TW |
| dc.subject | 前處理 | zh_TW |
| dc.subject | pretreatment | en |
| dc.subject | accessibility | en |
| dc.subject | crystallinity | en |
| dc.subject | enzyme hydrolysis | en |
| dc.subject | refining | en |
| dc.title | 不同前處理生質物對纖維素酶水解之影響 | zh_TW |
| dc.title | Effect of Pretreatments of Biomass on Enzymatic Hydrolysis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 施增廉,徐秀福(Hsiu-Fu Hsu) | |
| dc.subject.keyword | 前處理,可及性,結晶度,酵素水解,打漿, | zh_TW |
| dc.subject.keyword | pretreatment,accessibility,crystallinity,enzyme hydrolysis,refining, | en |
| dc.relation.page | 81 | |
| dc.identifier.doi | 10.6342/NTU201602877 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-17 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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