請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49375
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張靜文(Ching-Wen Chang) | |
dc.contributor.author | Yun-Tzu Ting | en |
dc.contributor.author | 丁韻滋 | zh_TW |
dc.date.accessioned | 2021-06-15T11:25:51Z | - |
dc.date.available | 2021-08-26 | |
dc.date.copyright | 2016-08-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-18 | |
dc.identifier.citation | Agranovski, I. E., Agranovski, V., Grinshpun, S. A., Reponen, T., & Willeke, K. (2002). Collection of airborne microorganisms into liquid by bubbling through porous medium. Aerosol Science & Technology, 36(4), 502-509.
An, H., Mainelis, G., & Yao, M. (2004). Evaluation of a high‐volume portable bioaerosol sampler in laboratory and field environments. Indoor air, 14(6), 385-393. Awad, A. H. A., Elmorsy, T. H., Tarwater, P. M., Green, C. F., & Gibbs, S. G. (2010). Air biocontamination in a variety of agricultural industry environments in Egypt: a pilot study. Aerobiologia, 26(3), 223-232. doi:10.1007/s10453-010-9158-y Bellanger, A.-P., Reboux, G., Scherer, E., Vacheyrou, M., & Millon, L. (2012). Contribution of a cyclonic-based liquid air collector for detecting Aspergillus fumigatus by QPCR in air samples. Journal of occupational and environmental hygiene, 9(1), D7-D11. Bernstein, J., Alexis, N., Bacchus, H., Bernstein, I. L., Fritz, P., Horner, E., . . . Tarlo, S. (2008). The health effects of nonindustrial indoor air pollution. Journal of Allergy and Clinical Immunology, 121(3), 585-591. Bettin, F., Montanari, Q., Calloni, R., Gaio, T. A., Silveira, M. M., & Dillon, A. J. (2009). Production of laccases in submerged process by Pleurotus sajor-caju PS-2001 in relation to carbon and organic nitrogen sources, antifoams and Tween 80. J Ind Microbiol Biotechnol, 36(1), 1-9. doi:10.1007/s10295-008-0463-1 Chang, C.-W., & Chou, F.-C. (2011). Assessment of Bioaerosol Sampling Techniques for ViableLegionella pneumophilaby Ethidium Monoazide Quantitative PCR. Aerosol science and technology, 45(3), 343-351. doi:10.1080/02786826.2010.537400 Chang, C. W., Chang, C. W., & Wang, L. J. (2015). Methods for quantifyingStaphylococcus aureusin indoor air. Indoor air, 25(1), 59-67. Cieplik, Z. (1997). Zagrzybienie księgozbioru Biblioteki Polonistycznej wydziału Filologicznego Uniwersytetu Śląskiego. Stud Bibliol, 10, 107-119. Deloge-Abarkan, M., Ha, T.-L., Robine, E., Zmirou-Navier, D., & Mathieu, L. (2007). Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH). Journal of Environmental Monitoring, 9(1), 91-97. Deshpande, M., Calenoff, E., & Daniels, L. (1995). Rapid large-scale growth of Helicobacter pylori in flasks and fermentors. Applied and environmental microbiology, 61(6), 2431-2435. Fischer, G., & Dott, W. (2003). Relevance of airborne fungi and their secondary metabolites for environmental, occupational and indoor hygiene. Archives of Microbiology, 179(2), 75-82. Fisk, W. J., Lei Gomez, Q., & Mendell, M. J. (2007). Meta-analyses of the associations of respiratory health effects with dampness and mold in homes. Indoor air, 17(4), 284-296. Giese, E., Covizzi, L., Dekker, R. F. H., & Barbosa, A. D. M. (2004). Influência de Tween na produção de lacases constitutivas e indutivas pelo Botryosphaeria sp. Acta Scientiarum. Biological Sciences, 26(4), 463. Godish, D. R., & Godish, T. J. (2007). Relationship between sampling duration and concentration of culturable airborne mould and bacteria on selected culture media. Journal of applied microbiology, 102(6), 1479-1484. Griffiths, W., Stewart, I., Reading, A., & Futter, S. (1996). Effect of aerosolisation, growth phase and residence time in spray and collection fluids on the culturability of cells and spores. Journal of aerosol science, 27(5), 803-820. Grinshpun, S., Chang, C.-W., Nevalainen, A., & Willeke, K. (1994). Inlet characteristics of bioaerosol samplers. Journal of aerosol science, 25(8), 1503-1522. Grinshpun, S., Willeke, K., Ulevicius, V., Juozaitis, A., Terzieva, S., Donnelly, J., . . . Brenner, K. (1997). Effect of Impaction, Bounce and Reaerosolization on the Collection Efficiency of Impingers. Aerosol science and technology, 26(4), 326-342. Han, T., & Mainelis, G. (2012). Investigation of inherent and latent internal losses in liquid-based bioaerosol samplers. Journal of aerosol science, 45, 58-68. Heseltine, E., & Rosen, J. (Eds.). (2009). WHO guidelines for indoor air quality: dampness and mould.: WHO Regional Office Europe. Hulme, M., & Stranks, D. (1970). Induction and the regulation of production of cellulase by fungi. Kane, A. V., & Plaut, A. G. (1996). Unique susceptibility of Helicobacter pylori to simethicone emulsifiers in alimentary therapeutic agents. Antimicrobial agents and chemotherapy, 40(2), 500-502. Karbowska-Berent, J., Górny, R. L., Strzelczyk, A. B., & Wlazło, A. (2011). Airborne and dust borne microorganisms in selected Polish libraries and archives. Building and Environment, 46(10), 1872-1879. doi:10.1016/j.buildenv.2011.03.007 Le Goff, O., Godon, J.-J., Steyer, J.-P., & Wéry, N. (2011). New specific indicators for qPCR monitoring of airborne microorganisms emitted by composting plants. Atmospheric environment, 45(30), 5342-5350. Li, C.-S., & Lin, Y.-C. (2001). Storage effects on bacterial concentration: determination of impinger and filter samples. Science of the total environment, 278(1), 231-237. Lin, W.-H., & Li, C.-S. (2003). Influence of Storage on the Fungal Concentration Determination of Impinger and Filter Samples. AIHA Journal, 64(1), 102-107. Lin, X., A. Reponen, T., Willeke, K., Grinshpun, S., Foarde, K., & Ensor, D. (1999). Long-term sampling of airborne bacteria and fungi into a non-evaporating liquid. Atmospheric environment, 33(26), 4291-4298. Lin, X., Reponen, T., Willeke, K., Wang, Z., Grinshpun, S., & Trunov, M. (2000a). Survival of Airborne Microorganisms During Swirling Aerosol Collection. Aerosol science and technology, 32(3), 184-196. Lin, X., Reponen, T., Willeke, K., Wang, Z., Grinshpun, S. A., & Trunov, M. (2000b). Survival of Airborne Microorganisms During Swirling Aerosol Collection. Aerosol science and technology, 32(3), 184-196. doi:10.1080/027868200303722 Lin, X., Willeke, K., Ulevicius, V., & Grinshpun, S. A. (1997). Effect of sampling time on the collection efficiency of all-glass impingers. American Industrial Hygiene Association Journal, 58(7), 480-488. Méheust, D., Le Cann, P., Reboux, G., Millon, L., & Gangneux, J.-P. (2014). Indoor fungal contamination: Health risks and measurement methods in hospitals, homes and workplaces. Critical Reviews in Microbiology, 40(3), 248-260. Méndez, J., & Mendez, J. (2004). Standardised evaluation of the performance of a simple membrane filtration-elution method to concentrate bacteriophages from drinking water. Journal of virological methods, 117(1), 19-25. May, K. R., & Harper, G. J. (1957). The efficiency of various liquid impinger samplers in bacterial aerosols. British journal of industrial medicine, 14(4), 287-297. McGinnis, M. (2004). Pathogenesis of indoor fungal diseases*. Medical mycology, 42(2), 107-117. Morales Morales, H., Vidal, G., Olszewski, J., Rock, C., Dasgupta, D., Oshima, K., & Smith, G. (2003). Optimization of a reusable hollow-fiber ultrafilter for simultaneous concentration of enteric bacteria, protozoa, and viruses from water. Applied and environmental microbiology, 69(7), 4098-4102. Morey, P. R. (1990). Practical aspects of sampling for organic dusts and microorganisms. American journal of industrial medicine, 18(3), 273-278. Mudarri, D., & Fisk, W. J. (2007). Public health and economic impact of dampness and mold. Indoor air, 17(3), 226-235. Pardo, A. G. (1996). Effect of surfactants on cellulase production by Nectria catalinensis. Current Microbiology, 33(4), 275-278. Piotrowska, M., Zielińska-Jankiewicz, K., Kozajda, A., & Gutarowska, B. (2006). Zanieczyszczenie powietrza grzybami strzępkowymi w archiwach i bibliotekach. Ochrona przed korozją, 9, 176-196. Polaczyk, A., Narayanan, J., Cromeans, T., Hahn, D., Roberts, J., Amburgey, J., & Hill, V. (2008). Ultrafiltration-based techniques for rapid and simultaneous concentration of multiple microbe classes from 100-L tap water samples. Journal of microbiological methods, 73(2), 92-99. Polaczyk, A., Roberts, J., & Hill, V. (2007). Evaluation of 1MDS electropositive microfilters for simultaneous recovery of multiple microbe classes from tap water. Journal of microbiological methods, 68(2), 260-266. Quansah, R., Jaakkola, M., Hugg, T., Heikkinen, S. A. M., Jaakkola, J. J. K., & Behrens, T. (2012). Residential Dampness and Molds and the Risk of Developing Asthma: A Systematic Review and Meta-Analysis. PLoS ONE, 7(11), e47526. Rancaño, G., Lorenzo, M., Molares, N., Rodrı́guez Couto, S., & Sanromán, M. Á. (2003). Production of laccase by Trametes versicolor in an airlift fermentor. Process Biochemistry, 39(4), 467-473. doi:10.1016/s0032-9592(03)00083-9 Reese, E., Lola, J., & Parrish, F. (1969). Modified substrates and modified products as inducers of carbohydrases. Journal of bacteriology, 100(3), 1151-1154. Reese, E., & Maguire, A. (1969). Surfactants as stimulants of enzyme production by microorganisms. Applied microbiology, 17(2), 242-245. Reynolds, S., Parker, D., Vesley, D., Janni, K., & McJilton, C. (1994). Occupational Exposure to Organic Dusts and Gases in the Turkey Growing Industry. Applied occupational and environmental hygiene, 9(7), 493-502. Riemenschneider, L., Woo, M. H., Lundgren, D., Wander, J., Wu, C. Y., Lundgren, D., . . . Heimbuch, B. (2010). Characterization of reaerosolization from impingers in an effort to improve airborne virus sampling. Journal of applied microbiology, 108(1), 315-324. Rule, A., Schwab, K., Kesavan, J., & Buckley, T. (2009). Assessment of Bioaerosol Generation and Sampling Efficiency Based onPantoea agglomerans. Aerosol science and technology, 43(6), 620-628. Samson, R. A., Flannigan, B., Flannigan, M. E., Verhoeff, A. P., Adan, O. C. G., & Hoekstra, E. S. (1994). Health implications of fungi in indoor environments.: Elsevier Science. Springorum, A. C., Clauss, M., Hartung, J., & Clauß, M. (2011). A Temperature-Controlled AGI-30 Impinger for Sampling of Bioaerosols. Aerosol science and technology, 45(10), 1231-1239. Thorne, P., Lange, J., Bloebaum, P., & Kullman, G. (1994). Bioaerosol Sampling in Field Studies: Can Samples be Express Mailed? AIHA Journal, 55(11), 1072-1079. Thorne, P. S., Kiekhaefer, M. S., Whitten, P., & Donham, K. J. (1992). Comparison of bioaerosol sampling methods in barns housing swine. Applied and environmental microbiology, 58(8), 2543-2551. Verreault, D., Gendron, L., Rousseau, G. M., Veillette, M., Massé, D., Lindsley, W. G., . . . Duchaine, C. (2011). Detection of airborne lactococcal bacteriophages in cheese manufacturing plants. Applied and environmental microbiology, 77(2), 491-497. Viegas, C., Faria, T., Gomes, A. Q., Sabino, R., Seco, A., & Viegas, S. (2014). Fungal Contamination in Two Portuguese Wastewater Treatment Plants. Journal of toxicology and environmental health. Part A, 77(1-3), 90-102. willeke, K., Lin, X., & Grinshpun, S. (1998). Improved Aerosol Collection by Combined Impaction and Centrifugal Motion. Aerosol science and technology, 28(5), 439-456. Xu, Z., & Yao, M. (2011). Analysis of culturable bacterial and fungal aerosol diversity obtained using different samplers and culturing methods. Aerosol science and technology, 45(9), 1143-1153. 解惟棠, & 夏滄琪. (2004). 國史館典藏庫房空中真菌相之調查. Biodegradation,53, 165-170. 楊心豪, 林宜長, 張振平, & 莊啟佑. (2009). 農業生技產業生物氣膠暴露特性調查 -以養鴨場爲例. 勞工安全衛生研究季刊, 17(4), 436-445. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49375 | - |
dc.description.abstract | 真菌廣泛存在於各式環境中,人體暴露於空氣中的真菌可引發過敏、呼吸道疾病與其他健康不良效應。為能評估人員的暴露風險,應使用有效的採樣方法搭配適當的樣本儲存方式,以正確定量空氣中真菌。以液體捕集真菌氣膠的採樣方法具有採樣時間較為彈性以及後續分析方法多元等優勢,然現今對於該方法效能評估之研究有限,且尚未被廣泛應用。
為找出針對真菌氣膠以液體為介質之適當定量方法,本研究使用BioSampler與Coriolis μ sampler兩種採樣器搭配8種不同配方之收集液,於室內環境中進行真菌氣膠採樣,同時使用BioStage與前兩種採樣器同步進行採樣,藉以校正不同環境與批次之環境真菌濃度差異,做為基準比較採樣器搭配收集液不同組合間之採樣效能。樣本均推於含氯黴素之麥芽抽出物培養基 (Malt Extract Agar with Chloramphenicol) 上經25℃培養7日後定量真菌濃度 (CFU/m3)。另為評估儲存溫度以及儲存時間對於真菌濃度之影響,採集之環境樣本另亦儲存於4℃與室溫下,並分別於2、4、6日後分析可培養真菌濃度之變化。研究使用之8種收集液其主要基質為磷酸鹽緩衝液 (phosphate buffer saline, PBS),並搭配有無添加非離子型介面活性劑 (Tween 20, Tween 80 或 Triton X-100) 以及消泡劑 (antifoam) 而組成。 本研究共進行10次環境採樣,收集160件環境樣本與160件現場空白樣本;另共分析1120件培養樣本,用於評估採集效率與儲存條件之影響。另外計算RBioStage值 (RBioStage = 液體採樣方法所得濃度/ BioStage採樣所得之濃度),再將RBioStage值取log轉換做為評估採樣效率之指標,若是某一個液體採樣組合之log RBioStage值越大 (即RBioStage值越大),表示採樣效率越高。結果顯示,BioSampler與Coriolis μ sampler相比具有較高之採集效率,其中又以使用Triton X-100之PBS收集液的採集效率最高 (平均log RBioStage = -0.19±0.29)。整體顯著影響採樣效率的因子包含採樣器種類 (P<0.0001)、收集液種類 (P<0.0001)、採樣地點風速 (P=0.0007)、採樣地點濕度 (P=0.02)。在儲存條件方面,將樣本經過不同條件儲存後之濃度 (CFU/mL) 以未儲存之真菌濃度 (CFU/mL) 為基準計算Rstorage值 (Rstorage = 儲存後之樣本濃度 / 未儲存之樣本濃度),再將Rstorage值取log轉換做為評估指標。log RStorage值離0越近 (即RStorage值離1越近),表示樣本經過儲存後濃度越接近採樣後未經儲存之濃度值,代表其較能反映環境中真菌之實態。結果顯示儲存時間增加,樣本濃度與未經儲存時之濃度差異增加,而儲存於4℃時之樣本濃度變異程度較低且較不受時間因素影響 (儲存2-6天平均log RStorage值 = -0.02±0.39),比室溫環境 (儲存2-6天平均log RStorage值 = 0.13±0.67) 適合存放樣本。顯著影響樣本儲存後濃度改變程度之因子包含樣本儲存時間與溫度 (P < 0.0001)、採樣器種類 (P < 0.0001)、收集液種類 (P < 0.0001)、地點 (P < 0.0001)。結合採樣效率與儲存效應進行液體定量真菌方法之評估結果,建議使用BioSampler搭配Triton X-100加PBS之收集液對於空氣中真菌進行採樣,採集回來的樣本若需儲存,則建議置於4℃之冷藏環境並於兩日內分析完畢。 | zh_TW |
dc.description.abstract | Adverse health effects and degradation of indoor air quality induced by fungi are growing concern. Among various sampling methods for fungi, liquid-based impinger provides multiple choices in sample analysis. However, this method has not been comprehensively assessed for its sampling efficiency (SE) and storage effect after collection. Therefore, this study aims to evaluate various liquid-based sampling methods to quantify airborne fungi and determine the significant factors affecting SE. In addition, effects of storage time and temperature on fungi concentration were also assessed.
Airborne fungi were sampled by two widely-used liquid samplers (BioSampler and Coriolis μ sampler) respectively loaded with eight types of collection fluid (contained phosphate buffer saline (PBS), surfactant and antifoam) in three types of location, including storage room, library and animal house. Besides, BioStage was used as reference to compare fungi concentration that collected by each collection fluid (N=160), in order to estimate SE. The present study use log RBioStage as the indicator of SE, which presents a log-transformed ratio of cell concentration in the air collected by liquid methods to that collected by BioStage. The higher the log RBioStage is, the higher the SE is. Effects of storage time (from 0 to 6 days) and temperature (4℃ and room temperature) were estimated by the ratio of fungi concentration in fluids stored for t and 0 day (N=1120). This study use log RStorage as indicator of storage effects, which presents a log-transformed ratio of cell concentration in the analytical sample (CFU/mL) right after sampling to that at various storage times. Sample concentration after storage is close to original concentration when the log RStorage close to 0, which means the results are representive. Generalized linear model (GLM) was used to estimate effects of different factors on SE and storage effect. The GLM results showed that SE was significantly influenced by sampler (P<0.0001), collection fluid type (P<0.0001), wind speed (P=0.0007) and relative humidity (P=0.02). Additionally, sampling by BioSampler with collection fluid which consisted of PBS and Triton X-100 showed the highest efficiency (average log RBioStage = -0.19±0.29). Moreover, sample concentration after storage was significantly affected by sampler (P < 0.0001), collection fluid type (P < 0.0001), sampling location (P < 0.0001), storage time and temperature (P < 0.0001). Fungi concentration tended to be overestimated as storage time increasing when stored at room temperature (average log RStorage = -0.02±0.39, stored for 2-6 days) but remain relatively stable at 4℃ (average log RStorage =0.13±0.67, stored for 2-6 days). Summarizing the results of sampling efficiency and storage effects, sampling by BioSampler with collection fluid consisted of PBS and Triton X-100 followed by sample storage at 4℃ is recommended. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:25:51Z (GMT). No. of bitstreams: 1 ntu-105-R03844006-1.pdf: 1929283 bytes, checksum: 84075586b06af44b876f9176066bfbb5 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 表目錄 4
圖目錄 7 第一章 前言 8 第二章 文獻回顧 9 2.1 空氣中真菌 9 2.2 空氣中真菌之定量方法 10 2.3 生物氣膠收集液成份 12 2.4 儲存效應(Storage effects) 14 第三章 研究目的 16 第四章 研究架構 17 第五章 材料與方法 19 5.1 材料 19 5.1.1採樣設備與採樣介質 19 5.1.2收集液及實驗用培養基 21 5.1.3 採樣設備清單 25 5.1.4 實驗室分析儀器清單 25 5.2環境採樣策略 26 5.2.1採樣地點與位置 26 5.2.2環境因子測量 26 5.2.3採樣前準備 29 5.2.4 採樣流程 29 5.3現場空白樣本 32 5.3.1收集液空白樣本 32 5.3.2 BioStage空白樣本 32 5.4運送條件 32 5.5收集液剩餘體積 32 5.6樣本前處理 33 5.7樣本分析 33 5.7.1 BioStage樣本分析 33 5.7.2採樣效率評估 33 5.7.3儲存效應評估 38 5.8偵測下限 (Detection limit, DL) 40 5.9統計分析 42 第六章 結果 43 6.1環境因子 43 6.2 偵測下限 45 6.3液體流失率 51 6.4 採樣效率(Sampling efficiency) 54 6.4.1 採樣器與收集液之影響 58 6.4.2 八種液體採樣方法與BioStage方法比較 60 6.4.3 採樣地點與採樣效率之關聯性 62 6.4.4 環境因子與採樣效率之關聯性 63 6.5 儲存效應 (Storage effect) 64 6.5.1 儲存溫度與時間 67 6.5.2 收集液種類之影響 71 6.5.3 採樣地點之影響 89 第七章 討論 91 7.1 偵測下限與陽性率 91 7.2 採樣效率之影響因子 95 7.2.1 液體流失率 95 7.2.2 採樣器種類 101 7.2.3 液體成份 102 7.2.4 環境因子 105 7.3 儲存效應 106 7.3.1 儲存溫度與時間 106 7.3.2 收集液種類 110 7.3.3 採樣地點 113 7.4 結合採樣效率與儲存效應之評估 116 第八章 研究限制與未來建議 120 8.1 研究限制 120 8.2 對未來研究之建議 120 第九章 結論 122 附錄 123 參考文獻 125 口試委員問題與回覆 131 | |
dc.language.iso | zh-TW | |
dc.title | 以液體方法定量空氣中真菌之效能評估 | zh_TW |
dc.title | Evaluation of liquid-based sampling methods to quantify airborne fungi | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 余國賓(Kuo-Pin Yu),陳乃慈(Nai-Tzu Chen) | |
dc.subject.keyword | 真菌,生物氣膠,收集液,採集效率,儲存效應, | zh_TW |
dc.subject.keyword | fungi,bioaerosol,collection fluid,sampling efficiency,storage effect, | en |
dc.relation.page | 135 | |
dc.identifier.doi | 10.6342/NTU201602896 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-18 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 環境衛生研究所 | zh_TW |
顯示於系所單位: | 環境衛生研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 1.88 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。