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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 梁國淦 | |
| dc.contributor.author | Dao-Ming Chang | en |
| dc.contributor.author | 張道明 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:14:16Z | - |
| dc.date.available | 2017-09-08 | |
| dc.date.copyright | 2016-09-08 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-21 | |
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Nature Methods, 2010. 7(11): 865-866. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49036 | - |
| dc.description.abstract | 於本研究中，為了量測貼覆型真核細胞的細胞膜力學構造，我建立了基於原子力顯微鏡 (Atomic force microscopy, AFM) 之測量與分析方法。由於量測時細胞膜和其底下細胞骨架可能同時被量測到，我利用與前人不同之分析方法，利用將力圖漸進分段分析之方式得到細胞表面之硬度分布。利用螢光標定位於細胞膜之膜蛋白或醣脂質後，利用將相同位置之螢光圖與硬度分佈相疊合的方式，將分子和硬度之分布相關聯。利用此方法，我測量了位於醣脂質 monosialotetrahexosyl- ganglioside (GM1) 以及膜蛋白GABAA subtype (alpha1)2(beta2)2 gamma2受體周圍之細胞膜硬度及高度，發現醣脂質 GM1 以及膜蛋白GABAA subtype (alpha1)2(beta2)2gamma2受體所在之位置，相較於周圍皆於較硬且較高。 | zh_TW |
| dc.description.abstract | In this study, I established an atomic force microscopy-based method to study the mechanical structure of the membrane of adherent eukaryotic cells. Since both the membrane and the underneath cytoskeletons could be simultaneously measured, I adapt a new analytical method for the force curve analysis. With a progressive truncation-refitting force curve analysis, the distribution of surface stiffness can be calculated. After the fluorescence labeling of sphingolipids or membrane protein, I correlate the stiffness and the molecular distribution by overlapping the fluorescence image with the stiffness distribution. With this method, I studied the membrane on the glycosphingolipid monosialotetrahexosylganglio (GM1) and the membrane around the membrane protein, gamma-aminobutyric acid type A receptor (GABAA receptor), subtype (alpha1)2(beta2)2 gamma2. I observed that both the GM1 and the GABAA receptor, subtype (alpha1)2(beta2)2 gamma2, are partitioned into a stiffer and higher domain than adjacent region. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:14:16Z (GMT). No. of bitstreams: 1 ntu-105-R03b22043-1.pdf: 6168111 bytes, checksum: 3f2249467ec6654382d6637bb74cd362 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
謝誌 II Contents III List of figures V List of table VII 中文摘要 VIII Abstract IX Chapter 1. Introduction 1 1.1. Membranes and general anesthesia 1 1.2. Mammalian plasma membrane structure 2 1.2.1. Major components of biological plasma membrane 2 1.2.2. Lipid raft hypothesis 7 1.2.3. Cytoskeleton-membrane interaction: fences-and-pickets model 13 1.3. HEK293T 16 1.4. AFM stiffness tomography 17 1.4.1. Working principle of AFM 17 1.4.2. Characteristics of a force-distance curve 21 1.4.3. Hertzian model: deriving stiffness from force-indentation curve 22 1.4.4. Stiffness tomography 24 1.5. GABAA receptors 27 1.6. Work plan 28 Chapter 2. Materials and methods 30 2.1 Cell Culture 30 2.2 Combined FL-AFM method with cytoskeleton, membrane (GM1) and protein (GABAA-R) labeling 30 2.2.1. Fluorescent Immunocytochemistry 31 2.2.2. AFM measurements 32 2.2.3. Data processing 32 2.3. Construction of AFM-ST algorithm 33 2.4. Exogenous expression of GABAA-R 34 2.4.1. Plasmid maintenance 34 2.4.2. Cell transfection with calcium phosphate method 35 2.4.3. Western blot 36 Chapter 3. Results 38 3.1 Expression level and spatial distribution of the GABAA receptor with a1b2g2 subunits 38 3.2 Parameters for HEK293T force mapping 42 3.3 Constructing the stiffness tomography (ST) algorithm 46 3.4 Correlating the distribution of GM1 molecules with surface stiffness 48 3.5 Membrane mechanical property around the GABAA receptor 54 Chapter 4. Discussions and conclusions 55 References 59 Appendices 63 Appendix 1. Calibration of the indentation from z-length and deflection 63 Appendix 2. The filter sets used in epifluorescence microscope 64 Appendix 3. The code of mathematica-based stiffness tomography 65 Appendix 4. The slides for thesis oral defense 66 | |
| dc.language.iso | en | |
| dc.subject | 硬度斷層成像 | zh_TW |
| dc.subject | force volume | zh_TW |
| dc.subject | 膜硬度 | zh_TW |
| dc.subject | GABA(A) 受體 | zh_TW |
| dc.subject | membrane stiffness | en |
| dc.subject | force volume | en |
| dc.subject | GABA(A) receptor | en |
| dc.subject | stiffness tomography | en |
| dc.title | 利用基於原子力顯微鏡之硬度斷層成像探測 HEK293T 細胞之膜力學構造 | zh_TW |
| dc.title | Probing the Membrane Mechanical Structure of HEK293T Cell by AFM-based Stiffness Tomography | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 魏培坤,周百里,余慈顏,楊啟伸 | |
| dc.subject.keyword | force volume,膜硬度,硬度斷層成像,GABA(A) 受體, | zh_TW |
| dc.subject.keyword | force volume,membrane stiffness,stiffness tomography,GABA(A) receptor, | en |
| dc.relation.page | 71 | |
| dc.identifier.doi | 10.6342/NTU201602741 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-21 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| Appears in Collections: | 生化科技學系 | |
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|---|---|---|---|
| ntu-105-1.pdf Restricted Access | 6.02 MB | Adobe PDF |
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