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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊健志(Chien-Chih Yang) | |
| dc.contributor.author | Chen-Hua Ma | en |
| dc.contributor.author | 馬蓁華 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:12:42Z | - |
| dc.date.available | 2021-08-25 | |
| dc.date.copyright | 2016-08-25 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-22 | |
| dc.identifier.citation | 高艾玲
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48972 | - |
| dc.description.abstract | 本研究室之前的研究指出，新穎阿拉伯芥血基質蛋白 AtMAPR3 (membrane associated progesterone receptor 3 in Arabidopsis thilana) 的基因表現量，會受外加的過氧化氫及茉莉花酸 (jasmonate, JA) 誘導而上升。此外，查詢微陣列資料庫，AtMAPR3 的表現量在許多逆境下，例如以葡萄灰黴菌 (Botrytis cinerea) 感染後，亦有受誘導而上升的現象。
為了進一步了解AtMAPR3 的生理功能是否和 ROS 產生之逆境有關，本研究比較野生型阿拉伯芥和 atmapr3-1 突變株在各種 ROS 生成逆境下的性狀差異，發現以 B. cinerea 感染五周大之阿拉伯芥植株，atmapr3-1 突變株葉部的感染面積 (lesion area) 約為野生型的 1.7 倍。另外，感染 B. cinerea 的兩天後，野生型阿拉伯芥中的 AtMAPR3 的表現量上升至約 11 倍。 進一步利用 qPCR 研究 AtMAPR3 的存在是否會影響 ROS 反應相關基因。將阿拉伯芥野生型和 atmapr3-1 突變株以 B. cinerea 感染之後，兩種病原菌誘導基因 PR5 (pathogenesis-related gene 5) 及 PDF1.2b (plant defensin 1.2b) 在野生型阿拉伯芥中，分別在 24 小時及 36 小時後，表現量分別上升約 5 倍及 3.5 倍。而在 atmapr3-1 突變株中，受誘導的表現量均有過敏感的現象 (分別達 11至 15倍)。此外，兩種 ROS 反應基因 TAT3 (tyrosine aminotransferase 3) 和 ZAT10 (salt tolerance zinc finger 10) 在以 B. cinerea 感染之後，在阿拉伯芥野生型中有和 atmapr3-1 突變株中呈現不同的表現模式。其中 TAT3 基因在野生型阿拉伯芥中，在感染後表現量逐漸上升，至 48 小時後達到最高 (約 14倍)。然而在 atmapr3-1 突變株中，感染 24 小時後表現量即達最高 (約 9 倍)。ZAT10 亦有類似的表現模式。這些結果顯示 AtMAPR3 的存在與否的確影響了許多 ROS 反應相關基因。 本研究顯示，在植物遭受病原菌侵害時，與其所引發的 ROS 訊息傳遞路徑 (ROS signaling pathway)，會促進 AtMAPR3 的表現，而 AtMAPR3 可能與該種逆境的生理反應相關。 | zh_TW |
| dc.description.abstract | It is known that the expression of AtMAPR3 (membrane associated progesterone receptor 3 in Arabidopsis thaliana), a novel heme binding protein, is induced by exogenesis hydrogen peroxide and jasmonate. As revealed from public microarray database, the expression of AtMAPR3 is upregulated under various biotic stresses, such as infection by Botrytis cinerea and Phytophthora infestans.
To further understand whether the physiological role of AtMAPR3 is related to ROS generating stresses, this study compare the phenotypic difference between wild type and a knock out mutant of AtMAPR3, atmapr3-1. It was found that the lesion area of atmapr3-1 mutant was larger than wild type by 1.7 fold when infected with B. cinerea. Also, the expression of AtMAPR3 in wild type was increased to 11 fold when infected for two days, indicate AtMAPR3 is responsive to the B. cinerea infection. To clarify if the presence of AtMAPR3 affects ROS responsive genes, the expression of these genes were examined in wild type or atmapr3-1 with B. cinerea infection. The expression of two pathogen responsive genes, PR5 (pathogenesis-related gene 5) and PDF1.2b (plant defensin 1.2b) in wild type infected with B. cinerea increased by 5 and 3.5 folds respectively after 24 hours and 36 hours. However, in atmapr3-1 mutants, the induced expression of those two genes were hypersensitive (reach to 11 and 15 folds respectively) to. Moreover, the expression of two ROS related genes TAT3 (tyrosine aminotransferase 3) and ZAT10 (salt tolerance zinc finger 10) in response to B. cinerea infection showed different expression patterns between wild type and atmapr3-1 mutant. The expression of TAT3 in wild type infected by B. cinerea increased along time and reached to its peak at 48 hours (about 14 folds). However in the atmapr3-1 mutant, the expression of those genes reached to its peak at 24 hours (about 9 folds). The ZAT gene response to B. cinerea infection showed similar expression pattern. These results implied the presence of AtMAPR3 affected the expression of some ROS responsive genes. I proposed that when plant infected by pathogens, the triggered ROS signaling pathway might induce the expression of AtMAP3, and the physiological role of AtMAPR3 might relate to this stress. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:12:42Z (GMT). No. of bitstreams: 1 ntu-105-R03b22033-1.pdf: 2489129 bytes, checksum: 3a8a97f3617c24335c7059b1a0ce79bb (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 目錄
摘要 I Abstract II 常用縮寫與全名對照表 III 目錄 VI Chapter 1緒論 1 1.1 血基質在植物體中扮演多種功能 1 1.2 ROS 之生成與清除可微調細胞體內之訊息 2 1.2.1 ROS 之種類與來源 2 1.2.2 植物中 ROS 的解毒 (detoxification) 3 1.2.3 ROS 作為訊息分子之功能與測定 4 1.2.4 過氧化酶及其功能 5 1.3 病原菌感染逆境 6 1.3.1 植物的防禦系統作用機制 6 1.3.2 葡萄灰黴菌 (B. cinerea)之作用機制 7 1.3.3 茉莉花酸功能與作用機制 7 1.4 次級細胞壁在植物病原菌防禦系統之功能 9 1.4.1 次級細胞壁相關代謝基因 9 1.4.2 次級細胞壁與植物防禦系統 10 1.5 MAPR family 在本研究室的相關研究成果 11 1.5.1 AtMAPR3 的基因表現 11 1.5.2 微陣列資料分析AtMAPR3 的生理功能 12 Chapter 2 材料與方法 13 2.1 植物材料 13 2.2 阿拉伯芥基因體 DNA (genomic DNA) 抽取 13 2.3 接種葡萄灰黴菌 14 2.4 抽取 RNA 及反轉錄 cDNA 14 2.5 即時定量聚合酶連鎖反應 (Quantitative real-time PCR, qPCR) 15 2.6 確認qPCR 產物 15 2.7分離原生質體 16 2.8 偵測 ROS 在原生質體中的表現 16 2.9 微矩陣列資列分析 16 2.10 熱處理 16 Chapter 3 結果 18 3.1 AtMAPR3 與高溫熱逆境 18 3.2 AtMAPR3 與病原菌感染 19 3.3 AtMAPR3 與創傷逆境 20 3.4 對AtMAPR3 突變株之轉錄體分析 20 3.4.1 轉錄因子 20 3.4.2 次級細胞壁代謝相關基因 21 3.4.3 逆境及病原菌誘導相關基因 21 3.4.4 ROS 反應基因 22 3.4.5 脂質及異戊二烯代謝相關基因 22 3.5 以 qPCR 分析突變株中相關差異化基因 22 3.5.1 次級細胞壁代謝相關基因之表現 22 3.5.2 逆境及病原菌誘導基因之表現 23 3.5.3 ROS 反應基因之表現 23 3.6 以 qPCR 分析經 B. cinerea 處理之相關目標基因 24 3.6.1 次級細胞壁代謝基因 24 3.6.2 逆境及病原菌誘導基因 24 3.6.3 ROS 反應基因 25 Chapter 4 討論與結論 26 4.1 AtMAPR3 的功能和若干 ROS 生成逆境密切相關 26 4.2 轉錄體分析顯示 AtMAPR3 缺失造成許多基因表現量的變化 27 4.2.1 轉錄因子 27 4.2.2 次級細胞壁代謝基因 28 4.2.3 逆境及病原菌誘導基因、ROS反應基因 28 4.3 AtMAPR3 可能的功能與其血基質結合特性密切相關 29 References 31 Figures…………………………………………………………………………….45 Table………………………………………………………………………………62 論文口試問答記錄 83 | |
| dc.language.iso | zh-TW | |
| dc.subject | 活性氧化物 | zh_TW |
| dc.subject | 葡萄灰黴菌 | zh_TW |
| dc.subject | 血基質蛋白質 | zh_TW |
| dc.subject | 植物防禦系統 | zh_TW |
| dc.subject | Botrytis | en |
| dc.subject | heme protein | en |
| dc.subject | ROS | en |
| dc.subject | plant defense | en |
| dc.title | 阿拉伯芥血基質蛋白 AtMAPR3 可能藉由活性氧化物
訊息路徑參與植物防禦反應 | zh_TW |
| dc.title | AtMAPR3, a heme protein from Arabidopsis, might play a role
in plant defense via the ROS signaling pathway | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王愛玉(AI-YU WANG),鄭秋萍(Chiu-Ping Cheng),常怡雍(Yee-yung Charng),陳佩燁(Rita P.-Y. Chen) | |
| dc.subject.keyword | 血基質蛋白質,活性氧化物,葡萄灰黴菌,植物防禦系統, | zh_TW |
| dc.subject.keyword | heme protein,ROS,Botrytis,plant defense, | en |
| dc.relation.page | 85 | |
| dc.identifier.doi | 10.6342/NTU201603533 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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