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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 曾國藩(Guo-Fang Tseng) | |
| dc.contributor.author | Li-Jin Chen | en |
| dc.contributor.author | 陳儷今 | zh_TW |
| dc.date.accessioned | 2021-06-15T07:05:34Z | - |
| dc.date.available | 2011-03-03 | |
| dc.date.copyright | 2011-03-03 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-12-04 | |
| dc.identifier.citation | Abe, T., Black, P.M. and Foley, L. (1984). Changes in parenchymal and ventricular pressure with experimental epidural compression. Surg. Neurol. 22, 477-480.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48631 | - |
| dc.description.abstract | 臨床醫學上，大腦在許多的病理情況如: 腦瘤、腦血腫、頭部外傷、水腦症等會有受到擠壓及壓迫的情形，但先前鮮少有研究針對此做探討。鑒於此，我們採用”大鼠硬腦膜外壓迫”的動物模式來探討物理性壓迫對於大腦神經元塑性的影響。我們在大鼠硬腦膜外，植入半圓形輕量硬物，壓迫大鼠體感覺運動區域上方。我們發現，物理性壓迫會立即造成壓迫源下方，大腦皮質內錐狀細胞的樹突發生擠壓及扭曲。在形態上此扭曲變形的樹突，會在持續壓迫3天後回復到原先平直的狀態。受壓迫的錐狀細胞在重新塑形的過程中，伴隨有微管相關蛋白(microtubule-associated proteins)磷酸化的增加。微管相關蛋白磷酸化的增加會使得細胞骨架產生不穩定的狀況，進而使細胞增加塑性以重組細胞形態。我們發現在壓迫後，微管相關蛋白MAP2和tau的磷酸化，分別在壓迫30分鐘到1小時以及壓迫10分鐘到12小時都有增加的情況發生。利用免疫染色方法，亦證實了磷酸化增加的MAP2和tau在受壓迫的錐狀細胞細胞體和樹突內表現量都上升。伴隨著微管相關蛋白磷酸化的增加，我們同時發現在壓迫10分鐘到壓迫1天，去磷酸酶-PP2A的酵素活性會降低，但是去磷酸酶-PP2B的酵素活性則沒有太大改變。在蛋白激酶方面，物理性壓迫會使得蛋白激酶-Erk1/2和p38的活性短暫增加。蛋白激酶和去磷酸酶的活性變化的時程，都和受到壓迫的細胞，形態重新塑形的時程相吻合。本實驗指出，物理性壓迫會立即引起細胞內蛋白激酶和去磷酸酶的活性平衡發生改變，進而導致微管相關蛋白磷酸化程度上升，使得受壓迫的細胞樹突內細胞骨架不穩定，導致壓迫後細胞形態上的改變。綜合上述，大腦受到壓迫時，很快的神經細胞即開始變化，進而造成神經細胞形態乃至於功能上的改變，因此臨床上因病變或外傷而引起腦壓迫時，宜儘速進行解壓迫，以避免細胞型態改變的分子在迅速的被啟動後，造成無法回復的變化。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T07:05:34Z (GMT). No. of bitstreams: 1 ntu-99-F93446001-1.pdf: 3813070 bytes, checksum: 3e7a7eb32b597ea18949c9b22a84e01e (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目次
中文摘要 iii Abstract vi I. Introduction 1 The Rat Epidural Compression Model 2 Microtubules 6 Microtubule-associated proteins (MAPs) 7 Phosphorylation of MAPs 10 Kinases and phosphatases of MAPs 11 II. Materials and methods 12 Epidural compression of cerebral cortex 13 Lysate preparation for SDS PAGE 14 Immunoblotting and analysis 14 Immunohistochemistry 15 Phosphatase activity assay 16 Statistical analysis 17 III. Results 18 Compression distorted microtubules in apical dendritic trunks 18 Compression increased MAP2 phosphorylation 18 Compression induced tau phosphorylation 19 Compression differentially altered the activities of several kinases 20 Compression downregulated protein phosphatase 2A activity specifically 21 IV. Discussion 22 Effects of compression on MAPs 22 Effects of compression on protein kinases and phosphatases 25 The significance of this study in the context of traumatic brain injury 29 V. Reference 33 VI. Figures and Figure legends 47 | |
| dc.language.iso | en | |
| dc.subject | 壓迫 | zh_TW |
| dc.subject | 體內實驗 | zh_TW |
| dc.subject | 神經塑性 | zh_TW |
| dc.subject | 頭部外傷 | zh_TW |
| dc.subject | tau 蛋白 | zh_TW |
| dc.subject | in vivo studies | en |
| dc.subject | compression | en |
| dc.subject | tau | en |
| dc.subject | traumatic brain injury | en |
| dc.subject | neuroplasticity | en |
| dc.title | 探討壓迫引起大腦皮質神經元樹突快速重組的分子機制: 大鼠皮質硬腦膜外壓迫動物模式 | zh_TW |
| dc.title | The molecular events underlying the compression-induced rapid cortical neuronal dendritic remodeling: an investigation in a rat epidural cerebral compression model | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 陳建榮(Jeng-Rung Chen),王慈娟(Tsyr-Jiuan Wang),王曰然(Yueh-Jan Wang),黃敏銓(Min-Chuan Huang) | |
| dc.subject.keyword | 體內實驗,神經塑性,頭部外傷,tau 蛋白,壓迫, | zh_TW |
| dc.subject.keyword | in vivo studies,neuroplasticity,traumatic brain injury,tau,compression, | en |
| dc.relation.page | 75 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-12-06 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
| 顯示於系所單位： | 解剖學暨細胞生物學科所 | |
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