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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王淑珍(Shu-Jen Wang) | |
| dc.contributor.author | Hui-Hsin Hsiao | en |
| dc.contributor.author | 蕭惠心 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:53:13Z | - |
| dc.date.available | 2012-09-08 | |
| dc.date.copyright | 2011-09-08 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-19 | |
| dc.identifier.citation | 參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48342 | - |
| dc.description.abstract | 高等植物體內，蔗糖從細胞間質裝載進入韌皮部進行運送及由韌皮部卸載至周圍細胞的過程中，蔗糖轉運蛋白 (sucrose transporters; SUTs) 扮演極重要的角色。水稻 (Oryza sativa L.) 蔗糖轉運蛋白基因家族成員為 OsSUT1、2、3、4及5。本研究主要藉由 OsSUT2 啟動子 POsSUT2::GUS 及 OsSUT4 啟動子 POsSUT4::GUS 轉殖水稻探討兩基因之組織專一性表現及生長發育上之差異表現。分析結果顯示 OsSUT2 及 OsSUT4 基因於根及葉部均具維管束韌皮部表現專一性；在穎花發育過程中，OsSUT2 及 OsSUT4 在抽穗前主要表現於內及外穎之穎脈，隨著抽穗、開花及授粉，穎脈專一性表現消失，轉而於雌蕊及雄蕊有明顯之表現。在充實穀粒中，OsSUT2 及 OsSUT4 主要在胚及糊粉層具顯著表現。在研究根系生長發育過程中，發現 OsSUT2 及 OsSUT4 啟動子主要於細胞生長快速之部位表現，例如種子根之根尖延長區及側根形成處。藉由外加醣類處理之試驗，得知 3% 之蔗糖及葡萄糖會促進 OsSUT2 及 OsSUT4 基因表現，而甘露醇與未處理醣類之表現差異不大。另外，利用 real-time RT-PCR 分析發現 OsSUT2 及 OsSUT4 兩基因之表現於機械傷害後 15 分鐘及 1 小時內即會被大量誘導，然而隨著時間增長，此兩基因之表現將逐漸下降。 | zh_TW |
| dc.description.abstract | In higher plants, sucrose transporters (SUTs) play important roles for the sucrose loading , which sucrose enters into phloem for further transportation and then unloads to surrounding cells. The rice (Oryza sativa L.) SUT gene family are composed of five members, OsSUT1 to 5. This study focused on the investigation of the tissue-specific and developmental regulation of OsSUT2 and OsSUT4 promoter in POsSUT2::GUS and POsSUT4::GUS transgenic rice plants. The results showed that GUS was specifically expressed in phloem tissue, both in OsSUT2 and OsSUT4. In developing caryopses, the activities of OsSUT2 and OsSUT4 promoters, were obviously observed in vascular bundles of lemma and palea at pre-heading stage. After heading and flowering, expression GUS in lemma and palea become undetectable but can be found in ovaries and anthers. In germinating seeds, OsSUT2 and OsSUT4 promoter activities were dominantly presented in embryos and aleurone layers. In developing roots, OsSUT2 and OsSUT4 promoters were both active in rapid growing regions, such as elongation zone of seminal roots and the sites of lateral root formation. Expression of OsSUT2 and OsSUT4 could be enhanced by 3% sucrose and glucose in root tissues. Real-time RT-PCR analysis showed that OsSUT2 and OsSUT4 mRNA levels in leaves would quickly increase after wounding treatment for 15 min and 1 hr, respectively, but following the mRNA levels declined gradually. | en |
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| dc.description.tableofcontents | 誌謝……………………………………………………………………............................i
中文摘要………………………………………………………………………………...ii Abstract………………………………………………………………………………....iii 目錄……………………………………………………………………………………..iv 表目錄…………………………………………………………………………………..vi 圖目錄………………………………………………………………………………….vii 附圖及附表目錄……………………………………………………………………......ix 縮寫字對照……………………………………………………………………………...x 前言 1. 水稻蔗糖轉運蛋白之生理功能及分類………………………………….…...….1 2. 水稻種子發芽時期內部醣類變化及運輸……………………………….…...….4 3. 水稻穎花內花粉發育過程及醣類運輸…………………………………...….….5 4. 醣類對水稻根系發育及側根生長之影響………………….……..………….….6 5. 醣類參與植物對傷害逆境之防禦機制..………………………………..…….....7 6. 本論文之研究主題………………………………………………………….....…8 材料與方法 1. 植物材料、種植及處理方法…………………...………….………………..……9 2. OsSUT2 及OsSUT4 啟動子:: GUS 轉殖水稻之分子鑑定..………..…….….….12 3. 轉殖水稻GUS 活性之組織化學染色分析….…………………….….…..….....13 4. 轉殖水稻之GUS 活性定量分析…..……………………………….……….......13 5. 即時定量RT-PCR 基因表現之分析…..…..………...……………….……..…..15 結果 1. OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻之分子鑑定…..……………..…..18 2. OsSUT2 及OsSUT4 啟動子-GUS 之組織專一性表現分析……………..........18 3. OsSUT2 及OsSUT4 啟動子於不同生育時期之活性表現分析……….………19 3.1. OsSUT2 及OsSUT4 啟動子於種子發芽生長時期之活性表現分析.…..19 3.2. OsSUT2 及OsSUT4 啟動子於穎花發育過程之活性表現分析…………20 3.3. 水稻花藥OsSUT1 ~ OsSUT5，蔗糖分解及澱粉合成相關基因之表現分 析………………………………………………………………………….20 4. 調控根系生長相關研究………………………….………………………..…….21 4.1. OsSUT4 啟動子於根系發育過程之活性表現分析………………..……. 21 4.2. 醣類對水稻根部OsSUT2 及OsSUT4 基因表現分析…...................…...22 5. 探討水稻蔗糖轉運蛋白基因受機械傷害所誘導之表現....……………...….....22 5.1. OsSUT2 及OsSUT4 基因於機械傷害處理下之表現分析……………...22 5.2. OsSUT4 啟動子受機械傷害誘導之活性定量分析…..…………………..23 討論 1. OsSUTs 基因家族之組織專一特性及不同發育時期調控機制……………...24 2. OsSUTs 基因於根部表現之調控機制……….……….…………………….....26 3. OsSUTs 與受傷防禦機制之探討……………………………………………...26 4. 結語與未來展望……………………………………………………………….28 參考文獻………….……………………………………………………………………29 表目錄 表一、OsSUT2 及OsSUT4 基因之比較….………………...…………………….…...37 圖目錄 圖一、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻之分子鑑定……………...….…38 圖二、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻於根尖處之GUS 組織化學染色 分析………………………………………...………………………………..39 圖三、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻於根尖處橫切面之GUS 組織化 學染色分析……………………………………………………...…………..40 圖四、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻葉片之GUS 組織化學染色分 析…….….…………………………………………………………………...41 圖五、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻葉鞘之GUS 組織化學染色分 析....................................................................................................………….42 圖六、 種子發芽時期之OsSUT 基因表現及啟動子活性分析.….………………...43 圖七、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻於種子發芽時期穀粒之GUS 組 織化學染色分析.. ….…………….…………….…………….……………..44 圖八、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻於穎花發育過程之GUS 組織化 學染色分析….……….…………….…………….…………….………..…..45 圖九、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻花藥內花粉之GUS 組織化學染 色分析…. ….…………….…………….…………….…………….………..46 圖十、 OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻與TNG67 穎花花藥內之花粉其 澱粉累積之表現…….…………….…………….…………….…………….47 圖十一、水稻花藥OsSUT1~OsSUT5、蔗糖分解及澱粉合成相關基因之表現分析 ………....…………………………………………………………………….48 圖十二、OsSUT4 啟動子-GUS 轉殖水稻根系之GUS 組織化學染色分析….........49 圖十三、OsSUT4 啟動子-GUS 轉殖水稻種子根之側根分化區之GUS 組織化學染色 分析.. ….…………….…………….…………….…………….…………….50 圖十四、OsSUT4 啟動子-GUS 轉殖水稻種子根側根分化區之橫切面及縱切面GUS 組織化學染色分析…………………………………………………...….…51 圖十五、水稻根系生長過程中OsSUT2 及OsSUT4 基因受醣類之外觀形態及基因表 現……………………………………….…………….………….………….52 圖十六、OsSUT2 及OsSUT4 啟動子-GUS 轉殖水稻之幼苗葉片以機械傷害後之GUS 組織化學染色分析……………………………... ….…………..…………..53 圖十七、TNG67 水稻幼苗葉部受機械傷害後OsSUT1、OsSUT2 及OsSUT4 基因表現分析……………………………………………………….…..…………..54 圖十八、POsSUT4::GUS 水稻幼苗之啟動子活性定量分析……………………….55 附表及附圖目錄 附表一、Genomic PCR 之專一性引子………………………………………..………56 附表二、Real-time RT-PCR 之專一性引子……………………………………….…..57 附表三、木村氏水耕液配方 …………………………………………………….…....60 附圖一、水稻蔗糖轉運蛋白之系統發生樹……………………………………..…...61 附圖二、OsSUT promoter::GUS 穩定性表現質體建構圖………………….….…....62 附圖三、OsSUT promoter-CYH10 質體建構圖………………………………......….63 附圖四、OsSUT2 基因啟動子序列………………………………………….....….....64 附圖五、OsSUT4 基因啟動子序列………………………………………….……….65 附圖六、POsSUT2 基因啟動子序列之cis-acting element 分析…….…..…….........66 附圖七、POsSUT4 基因啟動子序列之cis-acting element 分析…….……....……...67 | |
| dc.language.iso | zh-TW | |
| dc.subject | 機械傷害 | zh_TW |
| dc.subject | 蔗糖轉運蛋白 | zh_TW |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 啟動子 | zh_TW |
| dc.subject | 醣類 | zh_TW |
| dc.subject | wounding | en |
| dc.subject | sucrose transporter | en |
| dc.subject | rice (Oryza sativa L.) | en |
| dc.subject | promoter | en |
| dc.subject | sugars | en |
| dc.title | 水稻蔗糖轉運蛋白質基因OsSUT2及OsSUT4之表現分析 | zh_TW |
| dc.title | Analysis of expressions of rice sucrose transporter genes OsSUT2 and OsSUT4 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王愛玉(Ai-Yu Wang),黃文理(Wen-Lii Huang),張孟基(Men-Chi Chang),洪傳揚(Chwan-Yang Hong) | |
| dc.subject.keyword | 蔗糖轉運蛋白,水稻,啟動子,醣類,機械傷害, | zh_TW |
| dc.subject.keyword | sucrose transporter,rice (Oryza sativa L.),promoter,sugars,wounding, | en |
| dc.relation.page | 67 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-20 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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