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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47928完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張麗冠(Li-Kwan Chang) | |
| dc.contributor.author | Chien-Sin Chen | en |
| dc.contributor.author | 陳建炘 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:43:05Z | - |
| dc.date.available | 2016-07-25 | |
| dc.date.copyright | 2011-07-25 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-06 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47928 | - |
| dc.description.abstract | 極早期蛋白質Rta是促進Epstein-Barr virus (EBV) 進入溶裂循環時很重要的轉錄因子。Tripartite-motif 5 alpha (TRIM5alpha) 是反轉錄病毒限制因子 (restriction factor)，具有泛素E3連接酶 (ubiquitin E3 ligase) 的活性，在特定靈長類中能保護宿主免於反轉錄病毒 (retrovirus) 的侵害。然而，目前報導中，TRIM5alpha的限制目標大都限於反轉錄病毒，而鮮少有DNA病毒。本研究發現人類的TRIM5alpha能與Rta在細胞質直接結合。兩者的交互作用透過Rta的N端序列與TRIM5alpha C端的B30.2功能區而發生。另外，TRIM5alpha會促進Rta的泛素化，若過量表現TRIM5alpha也會導致Rta的濃度降低及下游的轉錄活化遭到抑制，進而緩解EBV的溶裂循環。綜合以上，本研究第一個發現人類TRIM5alpha作用於DNA病毒的蛋白質上，透過與Rta的結合並促進其泛素化，使EBV的溶裂循環受到抑制。 | zh_TW |
| dc.description.abstract | An immediate-early protein, Rta, is an important transcription factor required for initiating the lytic cycle of Epstein-Barr virus (EBV). Tripartite-motif 5 alpha (TRIM5alpha) is a restriction factor of retroviruses with the ubiquitin E3 ligase activity, which protects primates form retrovirus infection in a species-specific manner. However, the documented target spectrum of TRIM5alpha was limited to RNA viruses. This study finds that TRIM5alpha is a binding partner of Rta and colocalizes with Rta in the cytoplasm. The interaction involves the N-terminal region in Rta and the C-terminal B30.2 domain in TRIM5alpha. Moreover, overexpression of TRIM5alpha facilitates the ubiquitination of Rta and reduces the level of Rta, thereby inhibiting the transactivation activity of Rta. Collectively, this study identifies Rta to be the first DNA viral target of human TRIM5alpha, and the interaction prevents EBV lytic progression by promoting Rta's ubiquitination. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:43:05Z (GMT). No. of bitstreams: 1 ntu-100-R98B47402-1.pdf: 21432411 bytes, checksum: 3d3b666f7f4c87a40ee229b400f9de2b (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員審定書.............................................................................................................I
誌謝...............................................................................................................................II 中文摘要……………………………………………………………………………..III 英文摘要……………………………………………………………………………..IV 常用縮寫表…………………………………………………………………………...V 前言……………………………………………………………………………………1 一、 Epstein-Barr virus (EBV)…………………………………………………...1 二、 EBV的生活史……………………………………………………………...2 EBV的潛伏期…………………………………………………………………...2 EBV的溶裂循環………………………………………………………………...2 三、 EBV的極早期基因………………………………………………………...3 轉錄因子Zta……………………………………………………………………..3 Zta與宿主蛋白質的互動………………………………………………………..4 Zta的特殊上位基因 (epigenetic) 調控…………………………………...……5 轉錄因子Rta……………………………………………………………………..5 Rta與宿主蛋白質的互動………………………………………………………..6 Rta轉錄活性的調控……………………………………………………………..6 Rta與免疫系統的關聯…………………………………………………………..7 四、 EBV的溶裂複製…………………………………………………………...8 五、 泛素與泛素化 (ubiquitin and ubiquitination)……………………………...8 泛素與泛素化機制的發現………………………………………………………9 泛素鏈結系統 (ubiquitin-conjugating system) 的作用機制…………………..9 泛素化的連結方式……………………………………………………………..10 泛素化調控的生理反應………………………………………………………..11 病毒與宿主泛素系統的對抗…………………………………………………..12 六、 限制因子 (restriction factors) tripartite motif 5 alpha (TRIM5alpha)………..12 人類免疫缺乏病毒1型 (human immunodeficiency virus 1, HIV-1)…………13 TRIM5alpha對HIV-1的限制作用…………………………………………………14 TRIM5alpha蛋白質的功能區……………………………………………………...14 TRIM5alpha限制能力的物種特異性……………………………………………...15 TRIMCyp……………………………………………………………………….16 TRIM5alpha的表現與分佈………………………………………………………...17 細胞膜狀系統及藥物處理對TRIM5alpha限制HIV-1的影響…………………..17 TRIM5alpha與IFN及NF-kappaB的免疫反應路徑…………………………………..18 EBV對IFN及NF-kappaB免疫反應路徑的調控…………………………………18 研究目的……………………………………………………………………………..19 材料與方法…………………………………………………………………………..20 一、 菌種與細胞株……………………………………………………………..20 二、 質體與抗體………………………………………………………………..20 三、 大腸桿菌轉型作用 (transformation)……………………………………..21 四、 質體DNA的萃取…………………………………………………………21 五、 細胞轉染 (transfection)…………………………………………………..21 六、 蛋白質誘導表現…………………………………………………………..21 七、 Glutathione S-transferase (GST) pull down assay………………………...22 八、 免疫沉澱法 (immunoprecipitation, IP)…………………………………..22 九、 免疫螢光染色 (immunefluorescence staining, IF staining)……………...22 十、 變性免疫沉澱法 (denature immunoprecipitation, denature IP)………….23 十一、 冷光報導基因分析 (luciferase reporter assay)………………………......24 十二、 西方點墨分析 (western blot analysis)……………………………………24 結果…………………………………………………………………………………..26 一、 篩選細胞內與Rta可能結合的宿主蛋白質……………………………...26 二、 TRIM5alpha與Rta在細胞體內結合………………………………………….26 三、 TRIM5alpha與Rta結合位於細胞質………………………………………….27 四、 TRIM5alpha與Rta在細胞體外直接結合…………………………………….28 五、 TRIM5alpha以C端與Rta的N端結合……………………………………….28 六、 TRIM5alpha促進Rta的泛素化……………………………………………….29 七、 抑制TRIM5alpha的表現同時會降低Rta的泛素化…………………………30 八、 TRIM5alpha抑制Rta對EBV早期基因BMLF1及BMRF1啟動子上RRE的轉錄活化………………………………………………………………………30 九、 EBV進入溶裂循環時，TRIM5alpha能使Rta總量降低，進而降低下游基因的表現…………………………………………………………………………31 十、 TRIM5alpha所促進Rta的泛素化不會導致Rta進入lysosomal/autophagosomal degradation pathway……………………………..32 十一、 過量表現TRIM5alpha會突破MG132對Rta的穩定作用…………………...33 討論…………………………………………………………………………………..34 圖表…………………………………………………………………………………..41 表1、本研究使用之質體………………………………………………………….41 表2、本研究使用之抗體………………………………………………………….46 圖1、EBV的生活史................................................................................................47 圖2、細胞內與Rta結合的蛋白質……………………………………………….49 圖3、TRIM5alpha與Rta在細胞內的結合…………………………………………...50 圖4、TRIM5alpha與Rta的免疫螢光分析…………………………………………...51 圖5、TRIM5alpha與Rta的免疫螢光分析…………………………………………...52 圖6、TRIM5alpha與Rta的體外結合………………………………………………...53 圖7、TRIM5alpha以蛋白質C端與Rta的N端結合……………………………….54 圖8、TRIM5alpha促進Rta的泛素化………………………………………………...56 圖9、抑制TRIM5alpha時會減低Rta的泛素化…………………………...………...57 圖10、TRIM5alpha會降低Rta對BMLF1及BMRF1啟動子的轉錄活化………...59 圖11、TRIM5alpha會使Rta的濃度降低，進而使EA-D表現量下降…………….60 圖12、TRIM5alpha濃度過高時，會使泛素化Rta的濃度降低…………………….61 圖13、proteasome抑制劑MG132無法復原高濃度TRIM5alpha所造成的Rta濃度 下降…………………………………………………………………...………….62 圖14、TRIM5alpha與Rta結合後可能的模型及在EBV生活史轉換上的意義…...63 參考文獻......................................................................................................................64 附錄…………………………………………………………………………………..90 附錄1、泛素鏈結系統 (ubiquitin-conjugating system)………………………….90 附錄2、TRIM家族蛋白質的結構特徵..................................................................92 附錄3、TRIM5alpha限制HIV-1同時活化NF-kappaB免疫反應路徑.............................93 附錄4、TRIM家族蛋白質調節免疫系統..............................................................94 附錄5、質譜儀鑑定Rta在細胞內可能結合的蛋白質.........................................95 | |
| dc.language.iso | zh-TW | |
| dc.title | TRIM5alpha藉由促進Rta的泛素化緩解Epstein-Barr virus的溶裂循環 | zh_TW |
| dc.title | TRIM5alpha restrains Epstein-Barr virus lytic cycle by mediating ubiquitination of Rta | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉世東,張沛鈞,張世宗 | |
| dc.subject.keyword | Epstein-Barr virus (EBV),Rta,TRIM5alpha,泛素,泛素化,溶裂循環, | zh_TW |
| dc.subject.keyword | Epstein-Barr virus (EBV),Rta,TRIM5alpha,ubiquitin,ubiquitination,lytic cycle, | en |
| dc.relation.page | 96 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-06 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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