請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47792
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾顯雄(Shean-Shong Tzean) | |
dc.contributor.author | Wei-Chun Lin | en |
dc.contributor.author | 林暐峻 | zh_TW |
dc.date.accessioned | 2021-06-15T06:18:49Z | - |
dc.date.available | 2015-08-16 | |
dc.date.copyright | 2010-08-16 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-10 | |
dc.identifier.citation | 袁如陵, 2005. 靈芝A交配型基因座之選殖及功能界定. 碩士論文 國立台灣大學植物病理與微生物學研究所.
張東柱, 1983. 台灣數種靈芝生物學上之研究. 碩士論文 國立台灣大學植物病蟲害學研究所. 張東柱, 周文能, 2005. 野菇入門. 台北市: 遠流出版事業股份有限公司. 蔡宗統, 廖淑女, 1982. 靈芝之採集、栽培與利用. 台中市: 參雲出版社. Almeida, A. J., Martins, M., Carmona, J. A., Cano, L. E., Restrepo, A., Leao, C. & Rodrigues, F. (2006). New insights into the cell cycle profile of Paracoccidioides brasiliensis. Fungal Genet Biol 43, 401-409. Almeida, A. J., Matute, D. R., Carmona, J. A. & other authors (2007). Genome size and ploidy of Paracoccidioides brasiliensis reveals a haploid DNA content: flow cytometry and GP43 sequence analysis. Fungal Genet Biol 44, 25-31. Becker, W. M., Kleinsmith, L. J. & Hardin, J. (2006).The word of the cell. San Francisco: Benjamin Cummings Boh, B., Berovic, M., Zhang, J. & Zhi-Bin, L. (2007). Ganoderma lucidum and its pharmaceutically active compounds. Biotechnol Annu Rev 13, 265-301. Cano, M. I. N., Cisalpino, P. S., Galindo, I., Ramirez, J. L., Mortara, R. A. & da Silveira, J. F. (1998). Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis. J Clinl Microbiol 36, 742-747. Cantor, C. R., Gaal, A. & Smith, C. L. (1988). High resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA .3. Effect of electrical field shape. Biochemistry 27, 9216-9221. Chavez, R., Fierro, F., Gordillo, F., Martin, J. F. & Eyzaguirre, J. (2001). Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes. FEMS Microbiol Lett 205, 379-383. Chu, G., Vollrath, D. & Davis, R. W. (1986). Separation of large DNA molecules by contour clamped homogeneous electric fields. Science 234, 1582-1585. Cuadrado, A. & Jouve, N. (2007). Similarities in the chromosomal distribution of AG and AC repeats within and between Drosophila, human and barley chromosomes. Cytogenet Genome Res 119, 91-99. Cuadrado, A. & Jouve, N. (2010). Chromosomal detection of simple sequence repeats (SSRs) using nondenaturing FISH (ND-FISH). Chromosoma Online First, 1-9. Dean, R. A., Talbot, N. J., Ebbole, D. J. & other authors (2005). The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434, 980-986. Esser, K. (1995).The Mycota II Genetics and Biotechnology. New York: Springer-Verlag Berlin Heidelberg. Eusebio-Cope, A., Suzuki, N., Sadeghi-Garmaroodi, H. & Taga, M. (2009). Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica. Fungal Genet Biol 46, 342-351. Francis, D. M. & Michelmore, R. W. (1993). 2 Classes of chromosome sized molecules are present in Bremia lactucae. Experiment Mycol 17, 284-300. Galagan, J. E., Calvo, S. E., Borkovich, K. A. & other authors (2003). The genome sequence of the filamentous fungus Neurospora crassa. Nature 422, 859-868. Galagan, J. E., Calvo, S. E., Cuomo, C. & other authors (2005). Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438, 1105-1115. Garmaroodi, H. & Taga, M. (2007). Duplication of a conditionally dispensable chromosome carrying pea pathogenicity (PEP) gene clusters in Nectria haematococca. Mol Plant-Microbe Interact 20, 1495-1504. Gray, J., Carrano, A., Steinmetz, L., Van Dilla, M., Moore, D., Mayall, B. & Mendelsohn, M. (1975). Chromosome measurement and sorting by flow systems. Proc Nati Acad Sci 72, 1231. Gurrieri, S., Rizzarelli, E., Beach, D. & Bustamante, C. (1990). Imaging of kinked configurationa of DNA molecules undergoing orthogonal field alternating gel electrophoresis by fluorescence microscopy. Biochemistry 29, 3396-3401. Harper, L. & Zacheus Cande, W. (2000). Mapping a new frontier; development of integrated cytogenetic maps in plants. Funct Integr Genomics 1, 89-98. Herschleb, J., Ananiev, G. & Schwartz, D. C. (2007). Pulsed-field gel electrophoresis. Nat Protocols 2, 677-684. Hijri, M. & Sanders, I. R. (2004). The arbuscular mycorrhizal fungus Glomus intraradices is haploid and has a small genome size in the lower limit of eukaryotes. Fungal Genet Biol 41, 253-261. Horton, J. S. & Raper, C. A. (1991). Pulsed field gel electrophoretic analysis of Schizophyllum commune chromosomal DNA. Current Genetics 19, 77-80. Janda, J., Bartos, J., Safar, J. & other authors (2004). Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat. Theor Appl Genet 109, 1337-1345. Kubalakova, M., Kovarova, P., Suchankova, P., Cihalikova, J., Bartos, J., Lucretti, S., Watanabe, N., Kianian, S. F. & Dolezel, J. (2005). Chromosome sorting in tetraploid wheat and its potential for genome analysis. Genetics 170, 823-829. Laat, A. & Blaas, J. (1984). Flow-cytometric characterization and sorting of plant chromosomes. Theor Appl Genet 67, 463-467. Lalithakumari, D. (2000). Fungal Protoplast A Biotechnological Tool. Enfield: Science Publishers, Inc. Larraya, L., Perez, G., Penas, M., Baars, J., Mikosch, T., Pisabarro, A. & Ramirez, L. (1999). Molecular karyotype of the white rot fungus Pleurotus ostreatus. Appl Environ Microbiol 65, 3413-3417. Lewin, B. (2004). Genes VIII. New Jersey: Pearson Prentice Hall. Li, L., Gerecke, E. & Zolan, M. (1999). Homolog pairing and meiotic progression in Coprinus cinereus. Chromosoma 108, 384-392. Li, S., Harris, C. & Leong, S. (1993). Comparison of fluorescence in situ hybridization and primed in situ labeling methods for detection of single-copy genes in the fungus Ustilago maydis. Experiment Mycol 17, 301-308. Loftus, B. J., Fung, E., Roncaglia, P. & other authors (2005). The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science 307, 1321-1324. Lysak, M. A., Cihalikova, J., Kubalakova, M., Simkova, H., Kunzel, G. & Dolezel, J. (1999). Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.). Chrom Res 7, 431-444. Machida, M., Asai, K., Sano, M. & other authors (2005). Genome sequencing and analysis of Aspergillus oryzae. Nature 438, 1157-1161. Martinez, D., Larrondo, L. F., Putnam, N. & other authors (2004). Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nat Biotechnol 22, 695-700. Martinez, D., Berka, R. M., Henrissat, B. & other authors (2008). Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat Biotechnol 26, 553-560. Mathew, M. K., Hui, O. F., Smith, C. L. & Cantor, C. R. (1988a). High resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA .4. Influence of DNA topology. Biochemistry 27, 9222-9226. Mathew, M. K., Smith, C. L. & Cantor, C. R. (1988b). High resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA.1. DNA size standards and the effect of agarose and temperature. Biochemistry 27, 9204-9210. Mathew, M. K., Smith, C. L. & Cantor, C. R. (1988c). High resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA .2. Effect of pulse time and electric field strength and implications for models of the separation process. Biochemistry 27, 9210-9216. McGrath, C. L. & Katz, L. A. (2004). Genome diversity in microbial eukaryotes. Trends Ecol Evol 19, 32-38. Mehrabi, R., Taga, M. & Kema, G. H. J. (2007). Electrophoretic and cytological karyotyping of the foliar wheat pathogen Mycosphaerella graminicola reveals many chromosomes with a large size range. Mycologia 99, 868-876. Migheli, Q., Berio, T. & Gullino, M. (1993). Electrophoretic Karyotypes of Fusarium spp. Experiment Mycol 17, 329-337. Nierman, W. C., Pain, A., Anderson, M. J. & other authors (2005). Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438, 1151-1156. O'Gorman, C. M., Fuller, H. T. & Dyer, P. S. (2009). Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus. Nature 457, 471-474. Orbach, M. J., Vollrath, D., Davis, R. W. & Yanofsky, C. (1988). An electrophoretic karyotype of Neurospora crassa. Mol Cellul Biol 8, 1469-1473. Orbach, M. J., Chumley, F. G. & Valent, B. (1996). Electrophoretic karyotypes of Magnaporthe grisea pathogens of diverse grasses. Mol Plant-Microbe Interact 9, 261-271. Park, Y., Kim, J., Kong, W., Song, E., Lee, C., Kim, H., Hahn, J., Kang, H. & Lee, B. (2010). Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes. Microbiolog Res 165, 321-328. Pel, H. J., de Winde, J. H., Archer, D. B. & other authors (2007). Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat Biotechnol 25, 221-231. Safar, J., Bartos, J., Janda, J. & other authors (2004). Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat. Plant J 39, 960-968. Sankaran, K. V., Bridge, P. D. & Gokulapalan, C. (2005). Ganoderma diseases of perennial crops in India--an overview. Mycopathologia 159, 143-152. Schriml, L. M., Padilla-Nash, H. M., Coleman, A., Moen, P., Nash, W. G., Menninger, J., Jones, G., Ried, T. & Dean, M. (1999). Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27, 608-613. Schwartz, D. C. & Cantor, C. R. (1984). Separation of yeast chromosome sized DNAS by pulsed field gradient gel electrophoresis. Cell 37, 67-75. Schwarzacher, T. & Heslop-Harrison, P. (2000).Practical in situ Hybridization. New York: BIOS Scientific Publishers Ltd. Shiao, M. S. (2003). Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chemic Rec 3, 172-180. Shirane, N., Masuko, M. & Hayashi, Y. (1988). Nuclear behavior and division in germinating conidia of Botrytis cinerea. Phytopathology 78, 1627-1630. Shirane, N., Masuko, M. & Hayashi, Y. (1989). Light microscopic observation of nuclei and mitotic chromosomes of Botrytis species. Phytopathology 79, 728-730. Solieri, L., Cassanelli, S., Croce, M. A. & Giudici, P. (2008). Genome size and ploidy level: New insights for elucidating relationships in Zygosaccharomyces species. Fungal Genet Biol 45, 1582-1590. Sonnenberg, A. S. M., deGroot, P. W. J., Schaap, P. J., Baars, J. J. P., Visser, J. & vanGriensven, L. (1996). Isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes. Appl Environ Microbiol 62, 4542-4547. Taga, M. & Murata, M. (1994). Visualization of mitotic chromosomes in filamentous fungi by fluorescence staining and fluorescence in situ hybridization. Chromosoma 103, 408-413. Taga, M., Murata, M. & Saito, H. (1998). Comparison of different karyotyping methods in filamentous ascomycetes - a case study of Nectria haematococca. Mycolog Res 102, 1355-1364. Taga, M., Murata, M. & VanEtten, H. D. (1999). Visualization of a conditionally dispensable chromosome in the filamentous ascomycete Nectria haematococca by fluorescence in situ hybridization. Fungal Genet Biol 26, 169-177. Tsuchiya, D. & Taga, M. (2001). Cytological karyotyping of three Cochliobolus spp. by the germ tube burst method. Phytopathology 91, 354-360. Tsuchiya, D., Matsumoto, A., Covert, S. F., Bronson, C. R. & Taga, M. (2002). Physical mapping of plasmid and cosmid clones in filamentous fungi by fiber-FISH. Fungal Genet Biol 37, 22-28. Tsuchiya, D., Koga, H. & Taga, M. (2004). Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi. Mycologia 96, 208-210. Tsuchiya, D. & Taga, M. (2010). Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi. Methods Mol Biol 638, 235-257. Wang, C., Harper, L. & Cande, W. (2006). High-resolution single-copy gene fluorescence in situ hybridization and its use in the construction of a cytogenetic map of maize chromosome 9. The Plant Cell Online 18, 529. Wieloch, W. (2006). Chromosome visualisation in filamentous fungi. J Microbiol Methods 67, 1-8. Wood, V., Gwilliam, R., Rajandream, M. A. & other authors (2002). The genome sequence of Schizosaccharomyces pombe. Nature 415, 871-880. Zhong, S. B., Steffenson, B. J., Martinez, J. P. & Ciuffetti, L. M. (2002). A molecular genetic map and electrophoretic karyotype of the plant pathogenic fungus Cochliobolus sativus. Mol Plant-Microbe Interact 15, 481-492. Zhou, X., Lin, J., Yin, Y., Zhao, J., Sun, X. & Tang, K. (2007). Ganodermataceae: natural products and their related pharmacological functions. Am J Chin Med 35, 559-574. Zuccaro, A., Basiewicz, M., Zurawska, M., Biedenkopf, D. & Kogel, K.-H. (2009). Karyotype analysis, genome organization, and stable genetic transformation of the root colonizing fungus Piriformospora indica. Fungal Genet Biol 46, 543-550. Zwirglmaier, K. (2005). Fluorescence in situ hybridisation (FISH)--the next generation. FEMS Microbiol Lett 246, 151-158. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47792 | - |
dc.description.abstract | 靈芝(Ganoderma lucidum)為一種傳統的中藥材,同時靈芝也是一種會造成木材白腐的木材腐朽菌和多種樹木的病原菌。在近代的研究中證實靈芝的次級代謝產物具有抗腫瘤、降低高血壓和保護肝臟的功能。現今關於靈芝的研究很多,但大多都聚焦於靈芝的次級代謝產物上,在基礎研究上相對較為缺乏。本研究主要目的在探討靈芝的染色體特性,包含染色體核型分析以及功能性基因座落位置。在細胞學染色體核型分析,應用發芽管爆破的方式將靈芝單核株37180的染色體釋出,再以螢光染劑DAPI配合螢光顯微鏡觀察染色體的數量和型態。鏡檢顯示G. lucidum 37180 (A2B2)染色體數目為11至15條之間,平均為12.65條,長度在0.8 µm至4.1 µm之間。此外,電泳染色體核型分析則是用脈衝式電泳將染色體DNA依照分子量大小做分離,脈衝式電泳可將G. lucidum 37180的染色體DNA分離成六個訊號強度不同的條帶,大小分別為3.02、3.36、3.67、4.24、4.53和5.05 Mb。此外,也進行G. lucidum 37177(A1B1)的脈衝式電泳;37177的脈衝式電泳結果顯示具7個條帶,比37180多一個約4 Mb的條帶。將經脈衝式電泳分離的染色體DNA轉印到尼龍膜後進行南方氏雜合分析,以G. lucidum 37177 的13條染色體上的序列設計探針,可以在G. lucidum 37180脈衝式電泳的6個條帶上標示出與G. lucidum 37177同源性的13條染色體的位置,並可估計出G. lucidum 37180的基因體大小約為50.35 Mb。此外也利用專一性探針(A2之b1與B2之PR8基因)探測了G. lucidum 37180上交配型基因座的位置;A2交配型基因座位於ChI染色體上,B2交配型基因座則位於ChIII、ChV或ChVI其中一條染色體上。此外也嘗試應用掃描式電子顯微鏡、螢光原位雜交和流式細胞分析儀對G. lucidum染色體做分析。 | zh_TW |
dc.description.abstract | In nature, Ganoderma licidum acts as wood white-rot pathogens for many tree species; nevertheless, its fruit body has also been used for century as folk medicine. More recently, researches have shown its polysaccharides or terpenoides with anti-tumor, alleviating high blood pressure and hepatoprotective activity. Though immensive biological activity studies of G. lucidum have been focused on its secondary metabolites, the basic exploration appeared rare. Herein, we concentrated our efforts to study the chromosomal and karyotyping of G. lucidum via germ tube bursting method (GTBM), pulse field gel electrophoresis (PFGE), fluorescence in situ hybridization (FISH), flow cytometry and scanning electromicroscopy. GTBM in combination of fluorescent microscopy indicated that G. lucidum 37180 (A2B2) possessed 11-15 chromosomes, with average of 12.65, and a length of 0.8~4.1 µm, while PFGE showed the six-banded chromosomal zones of 3.02, 3.36, 3.67, 4.24, 4.53 and 5.05 Mb. One additional chromosomal band at 4.0 Mb was found in G. lucidum 37177 (A1B1). Southern blotting of the six GL 37180 chromosomes bands separated by PFGE by using specific probes derived from the thirteen deciphed GL 37177 chromosomes unraveled the 13 allelic chromosomes, respectively. In addition, by specific probe (A2: b1 gene) also can allocate GL A2 mating locus located at chromosome I, also allocating the possible location of B2 mating locus possibly at chromosome III, V or VI by specific probe (B2: PR8 gene). The same approaches undertaken in this study perhaps can be applied to study the karyotyping of other GL monokaryotic strains and other medicinal mushrooms as well. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:18:49Z (GMT). No. of bitstreams: 1 ntu-99-R96633006-1.pdf: 3274562 bytes, checksum: 5281a075c39d3f55b69fee954c456646 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iv 目錄 vi 表目錄 ix 圖目錄 x 壹、前言 1 貳、前人研究 3 靈芝簡介 3 基因體(genome)簡介 5 染色體核型分析(Karyotyping) 8 染色體圖譜(chromosome map) 18 靈芝染色體相關研究 22 参、材料與方法 23 靈芝菌株來源、培養、確認與保存 23 靈芝基因體DNA之抽取與分析 25 聚合酶鏈鎖反應之分析及產物純化 27 靈芝原生質體製備 31 Cytological karyotyping 34 螢光原位雜合(FISH) 35 Electrophoretic karyotyping 38 南方氏雜合分析 (Southern hybridization) 40 流式細胞儀分析(flow cytometry) 43 肆、結果 45 靈芝單核株交配型測試 45 靈芝原生質體製備及原生質體再生 45 Cytological karyotyping 46 Electrophoretic karyotyping 48 流式細胞儀分析 51 伍、討論 54 原生質體製備 54 Cytological karyotyping 55 Electrophoretic karyotyping 58 Flow Cytometry 62 未來展望 64 陸、圖表 66 柒、參考文獻 102 附錄、實驗試劑配方 110 靈芝菌株來源、培養、確認與保存 110 靈芝基因體DNA之抽取與分析 111 聚合酶鏈鎖反應之分析及產物純化 111 原生質體製備 112 Cytological karyotyping 112 螢光原位雜合(FISH) 113 Electrophoretic karyotyping 115 南方氏雜合分析 (Southern hybridization) 116 流式細胞儀分析(flow cytometry) 118 | |
dc.language.iso | zh-TW | |
dc.title | 靈芝染色體核型及功能性基因座落位置之分析 | zh_TW |
dc.title | Karyotyping and localization of functional genes on chromosomes of Ganoderma lucidum | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉瑞芬,張雅君,林乃君 | |
dc.subject.keyword | 靈芝,染色體,染色體核型分析,發芽管爆破,脈衝式電泳, | zh_TW |
dc.subject.keyword | Ganoderma lucidum,chromosome,karyotyping,germ tube burst method,pulsed field gel electrophore, | en |
dc.relation.page | 118 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-11 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 3.2 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。