請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47491完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林中天 | |
| dc.contributor.author | Pei-Shaw Ko | en |
| dc.contributor.author | 柯沛劭 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:02:34Z | - |
| dc.date.available | 2010-08-20 | |
| dc.date.copyright | 2010-08-20 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-16 | |
| dc.identifier.citation | References
Baratz KH, Nau CB, Winter EJ, McLaren JW, Hodge DO, Herman DC and Bourne WM. 2006. Effects of glaucoma medications on corneal endothelium, keratocytes, and subbasal nerves among participants in the ocular hypertension treatment study. Cornea 25(9): 1046-1052. Blocker T and Van Der Woerdt A. 2001. A comparison of corneal sensitivity between brachycephalic and Domestic Short-haired cats. Vet Ophthalmol 4(2): 127-130. Bohnke M and Masters BR. 1999. Confocal microscopy of the cornea. Prog Retin Eye Res 18(5): 553-628. Cafaro TA, Ortiz SG, Maldonado C, Esposito FA, Croxatto JO, Berra A, Ale OL, Torrealday JI, Urrets-Zavalia EA, Urrets-Zavalia JA and Serra HM. 2009. The cornea of Guinea pig: structural and functional studies. Vet Ophthalmol 12(4): 234-241. Chang PY, Carrel H, Huang JS, Wang IJ, Hou YC, Chen WL, Wang JY and Hu FR. 2006. Decreased density of corneal basal epithelium and subbasal corneal nerve bundle changes in patients with diabetic retinopathy. Am J Ophthalmol 142(3): 488-490. Croft AP and Przyborski SA. 2006. Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture. Stem Cells 24(8): 1841-1851. Erie JC, McLaren JW, Hodge DO and Bourne WM. 2005. The effect of age on the corneal subbasal nerve plexus. Cornea 24(6): 705-709. Erie JC, McLaren JW and Patel SV. 2009. Confocal microscopy in ophthalmology. Am J Ophthalmol 148(5): 639-646. Espana EM, Kawakita T, Romano A, Di Pascuale M, Smiddy R, Liu CY and Tseng SC. 2003. Stromal niche controls the plasticity of limbal and corneal epithelial differentiation in a rabbit model of recombined tissue. Invest Ophthalmol Vis Sci 44(12): 5130-5135. Gekka M, Miyata K, Nagai Y, Nemoto S, Sameshima T, Tanabe T, Maruoka S, Nakahara M, Kato S and Amano S. 2004. Corneal epithelial barrier function in diabetic patients. Cornea 23(1): 35-37. Grierson I, Heathcote L, Hiscott P, Hogg P, Briggs M and Hagan S. 2000. Hepatocyte growth factor/scatter factor in the eye. Prog Retin Eye Res 19(6): 779-802. Grupcheva CN, Wong T, Riley AF and McGhee CN. 2002. Assessing the sub-basal nerve plexus of the living healthy human cornea by in vivo confocal microscopy. Clin Experiment Ophthalmol 30(3): 187-190. Jalbert I, Stapleton F, Papas E, Sweeney DF and Coroneo M. 2003. In vivo confocal microscopy of the human cornea. Br J Ophthalmol 87(2): 225-236. Kafarnik C, Fritsche J and Reese S. 2007. In vivo confocal microscopy in the normal corneas of cats, dogs and birds. Vet Ophthalmol 10(4): 222-230. Kafarnik C, Fritsche J and Reese S. 2008. Corneal innervation in mesocephalic and brachycephalic dogs and cats: assessment using in vivo confocal microscopy. Vet Ophthalmol 11(6): 363-367. Kallinikos P, Berhanu M, O'Donnell C, Boulton AJ, Efron N and Malik RA. 2004. Corneal nerve tortuosity in diabetic patients with neuropathy. Invest Ophthalmol Vis Sci 45(2): 418-422. Kawakita T, Espana EM, He H, Li W, Liu CY and Tseng SC. 2005. Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure. Am J Pathol 167(2): 381-393. Ledbetter EC and Scarlett JM. 2009. In vivo confocal microscopy of the normal equine cornea and limbus. Vet Ophthalmol 12 Suppl 1: 57-64. Li W, Hayashida Y, Chen YT and Tseng SC. 2007. Niche regulation of corneal epithelial stem cells at the limbus. Cell Res 17(1): 26-36. Malik RA. 2003. Current and future strategies for the management of diabetic neuropathy. Treat Endocrinol 2(6): 389-400. Malik RA, Kallinikos P, Abbott CA, van Schie CH, Morgan P, Efron N and Boulton AJ. 2003. Corneal confocal microscopy: a non-invasive surrogate of nerve fibre damage and repair in diabetic patients. Diabetologia 46(5): 683-688. Mann T. 1944. Studies on the metabolism of mould fungi: 1. Phosphorus metabolism in moulds. Biochem J 38(4): 339-345. McLaren JW, Nau CB, Patel SV and Bourne WM. 2007. Measuring corneal thickness with the ConfoScan 4 and z-ring adapter. Eye Contact Lens 33(4): 185-190. Midena E, Brugin E, Ghirlando A, Sommavilla M and Avogaro A. 2006. Corneal diabetic neuropathy: a confocal microscopy study. J Refract Surg 22(9 Suppl): S1047-1052. Mocan MC, Durukan I, Irkec M and Orhan M. 2006. Morphologic alterations of both the stromal and subbasal nerves in the corneas of patients with diabetes. Cornea 25(7): 769-773. Patel DV and McGhee CN. 2006. Mapping the corneal sub-basal nerve plexus in keratoconus by in vivo laser scanning confocal microscopy. Invest Ophthalmol Vis Sci 47(4): 1348-1351. Patel DV, Tavakoli M, Craig JP, Efron N and McGhee CN. 2009. Corneal sensitivity and slit scanning in vivo confocal microscopy of the subbasal nerve plexus of the normal central and peripheral human cornea. Cornea 28(7): 735-740. Patel SV, Erie JC, McLaren JW and Bourne WM. 2007. Confocal microscopy changes in epithelial and stromal thickness up to 7 years after LASIK and photorefractive keratectomy for myopia. J Refract Surg 23(4): 385-392. Pearton DJ, Yang Y and Dhouailly D. 2005. Transdifferentiation of corneal epithelium into epidermis occurs by means of a multistep process triggered by dermal developmental signals. Proc Natl Acad Sci U S A 102(10): 3714-3719. Rosen EM, Nigam SK and Goldberg ID. 1994. Scatter factor and the c-met receptor: a paradigm for mesenchymal/epithelial interaction. J Cell Biol 127(6 Pt 2): 1783-1787. Rosenberg ME, Tervo TM, Immonen IJ, Muller LJ, Gronhagen-Riska C and Vesaluoma MH. 2000. Corneal structure and sensitivity in type 1 diabetes mellitus. Invest Ophthalmol Vis Sci 41(10): 2915-2921. Saghizadeh M, Brown DJ, Castellon R, Chwa M, Huang GH, Ljubimova JY, Rosenberg S, Spirin KS, Stolitenko RB, Adachi W, Kinoshita S, Murphy G, Windsor LJ, Kenney MC and Ljubimov AV. 2001. Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations. Am J Pathol 158(2): 723-734. Saghizadeh M, Chwa M, Aoki A, Lin B, Pirouzmanesh A, Brown DJ, Ljubimov AV and Kenney MC. 2001. Altered expression of growth factors and cytokines in keratoconus, bullous keratopathy and diabetic human corneas. Exp Eye Res 73(2): 179-189. Saghizadeh M, Kramerov AA, Tajbakhsh J, Aoki AM, Wang C, Chai NN, Ljubimova JY, Sasaki T, Sosne G, Carlson MR, Nelson SF and Ljubimov AV. 2005. Proteinase and growth factor alterations revealed by gene microarray analysis of human diabetic corneas. Invest Ophthalmol Vis Sci 46(10): 3604-3615. Seigel GM, Sun W, Salvi R, Campbell LM, Sullivan S and Reidy JJ. 2003. Human corneal stem cells display functional neuronal properties. Mol Vis 9: 159-163. Stachs O, Knappe S, Zhivov A, Kraak R, Stave J and Guthoff RF. 2006. [Three-dimensional confocal laser scanning microscopy of the corneal nerve structure]. Klin Monbl Augenheilkd 223(7): 583-588. Tavakoli M, Hossain P and Malik RA. 2008. Clinical applications of corneal confocal microscopy. Clin Ophthalmol 2(2): 435-445. Tavakoli M, Kallinikos PA, Efron N, Boulton AJ and Malik RA. 2007. Corneal sensitivity is reduced and relates to the severity of neuropathy in patients with diabetes. Diabetes Care 30(7): 1895-1897. Tavakoli M, Quattrini C, Abbott C, Kallinikos P, Marshall A, Finnigan J, Morgan P, Efron N, Boulton AJ and Malik RA. 2010. Corneal Confocal Microscopy: A Novel Non-invasive Test to Diagnose and Stratify the Severity of Human Diabetic Neuropathy. Diabetes Care. Tervo T and Moilanen J. 2003. In vivo confocal microscopy for evaluation of wound healing following corneal refractive surgery. Prog Retin Eye Res 22(3): 339-358. Wiley L, SundarRaj N, Sun TT and Thoft RA. 1991. Regional heterogeneity in human corneal and limbal epithelia: an immunohistochemical evaluation. Invest Ophthalmol Vis Sci 32(3): 594-602. Wilson SE, He YG, Weng J, Li Q, McDowall AW, Vital M and Chwang EL. 1996. Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin-1 system in the modulation of corneal tissue organization and wound healing. Exp Eye Res 62(4): 325-327. Wilson SE, He YG, Weng J, Zieske JD, Jester JV and Schultz GS. 1994. Effect of epidermal growth factor, hepatocyte growth factor, and keratinocyte growth factor, on proliferation, motility and differentiation of human corneal epithelial cells. Exp Eye Res 59(6): 665-678. Wilson SE, Mohan RR, Ambrosio R, Jr., Hong J and Lee J. 2001. The corneal wound healing response: cytokine-mediated interaction of the epithelium, stroma, and inflammatory cells. Prog Retin Eye Res 20(5): 625-637. Xu KP, Li Y, Ljubimov AV and Yu FS. 2009. High glucose suppresses epidermal growth factor receptor/phosphatidylinositol 3-kinase/Akt signaling pathway and attenuates corneal epithelial wound healing. Diabetes 58(5): 1077-1085. Zhao X, Das AV, Thoreson WB, James J, Wattnem TE, Rodriguez-Sierra J and Ahmad I. 2002. Adult corneal limbal epithelium: a model for studying neural potential of non-neural stem cells/progenitors. Dev Biol 250(2): 317-331. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47491 | - |
| dc.description.abstract | 在人類及獸醫眼科學中，眼角膜細胞的再生與修復在眼角膜傷口的癒合上扮演著重要的角色，活體共軛焦顯微鏡為一非侵入式的儀器，可以用來了解眼角膜傷口癒合過程中細胞的變化，本研究中的糖尿病病人接受玻璃體切除術前，會先施行眼角膜上皮清創步驟，活體共軛顯微鏡用以觀察並記錄術前、術後一週、兩週、三週、四週、三個月、六個月，眼角膜細胞及神經型態變化，觀察眼角膜基底上皮細胞、表層上皮細胞、基質層、內皮細胞、基底層下神經的型態，也以量化方式對內皮細胞進行細胞密度計算。在術後一個月、三個月、六個月，基底上皮癒合不全的比例為72.1%、15.2% 、0%，表層上皮癒合不全的比例為81.1%、9.1%、0%，結果顯示糖尿病患角膜再生過程較正常個體緩慢，基底層下神經彎曲的型態變化在糖尿病病人跟STZ誘發糖尿病症大鼠的活體共軛焦顯微鏡，皆可觀察到。 | zh_TW |
| dc.description.abstract | Corneal regeneration plays an important role in corneal wound healing in human and veterinary ophthalmology. In vivo confocal microscopy was used to observe the corneal epithelial cells during corneal wound healing. In the present study, the corneal wounds of diabetic patients who underwent corneal epithelial debridement during pars plana vitrectomy for proliferative diabetic retinopathy were investigated. In vivo confocal microscopy was used to evaluate images of the corneal basal and apical surface epithelial cells before surgery weekly for the first month, and at the third and sixth month after surgery, respectively. In vivo confocal microscopy showed incomplete healing of basal epithelial cells in 72.1%, 15.2%, and 0% of eyes and incomplete healing of surface apical epithelial cells in 81.1%, 9.1%, and 0% of eyes at 1, 3, and 6 months after surgery, respectively. The tortuous morphology of subbasal corneal nerves were observed in diabetic patients and STZ-induced diabetic rats. The results revealed the slower corneal regeneration process in the diabetic patients than non-diabetic patients. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:02:34Z (GMT). No. of bitstreams: 1 ntu-99-R96643012-1.pdf: 1335220 bytes, checksum: 7809362aa0e6af3da4ac4154166b23fe (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 中文摘要 iii
英文摘要 iv 第一章 緒論 1 第一節 眼角膜上皮細胞的再生 1 第二節 活體共軛焦顯微鏡 2 第三節 研究目的 3 第二章 文獻探討 4 第一節 眼角膜細胞的修復機制 4 第二節 糖尿病角膜病變 6 第三節 活體共軛焦顯微鏡於眼角膜的應用 8 第三章 實驗材料與方法 11 第一節 實驗材料 11 第二節 實驗方法 13 一、 活體共軛焦顯微鏡 13 二、 統計分析方法 14 第四章 實驗結果 15 第一節 以活體共軛焦顯微鏡觀察人類眼角膜 15 一、 以活體共軛焦顯微鏡觀察角膜上皮基底細胞 15 二、 以活體共軛焦顯微鏡觀察角膜頂端表層細胞 16 三、 以活體共軛焦顯微鏡分析基質層反應 16 四、 角膜內皮細胞密度分析 16 五、 以活體共軛焦顯微鏡分析基底細胞層下神經 17 第二節 以活體共軛焦顯微鏡觀察大鼠角膜 18 第五章 討論 19 第一節 角膜上皮細胞再生機制 19 第二節 糖尿病角膜傷口癒合之型態學討論 21 第三節 糖尿病角膜傷口延遲癒合討論 24 第四節 活體共軛焦顯微鏡於實驗室及臨床上的應用 25 第六章 結論 27 參考文獻 28 圖目錄 33 表目錄 44 | |
| dc.language.iso | zh-TW | |
| dc.subject | 活體共軛焦顯微鏡 | zh_TW |
| dc.subject | 角膜傷口癒合 | zh_TW |
| dc.subject | 糖尿病 | zh_TW |
| dc.subject | diabetics | en |
| dc.subject | in vivo confocal microscopy | en |
| dc.subject | corneal wound healing | en |
| dc.title | 以活體共軛焦顯微鏡探討眼角膜傷口癒合 | zh_TW |
| dc.title | Investigation of Corneal Wound Healing
by in vivo Confocal Microscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 陳偉勵 | |
| dc.contributor.oralexamcommittee | 林大盛,劉振軒 | |
| dc.subject.keyword | 活體共軛焦顯微鏡,角膜傷口癒合,糖尿病, | zh_TW |
| dc.subject.keyword | in vivo confocal microscopy,corneal wound healing,diabetics, | en |
| dc.relation.page | 46 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-17 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床動物醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床動物醫學研究所 | |
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