請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47300
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 閔明源(Ming-Yuan Min) | |
dc.contributor.author | Hsiao-Hsuan Chen | en |
dc.contributor.author | 陳曉萱 | zh_TW |
dc.date.accessioned | 2021-06-15T05:54:07Z | - |
dc.date.available | 2015-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-18 | |
dc.identifier.citation | References
Almeida TF, Roizenblatt S, Tufik S. 2004. Afferent pain pathways: a neuroanatomical review. Brain Res 1000:40-56. Bajic D, Proudfit HK. 1999. Projections of neurons in the periaqueductal gray to pontine and medullary catecholamine cell groups involved in the modulation of nociception. J Comp Neurol 405:359-379. Bajic D, Van Bockstaele EJ, Proudfit HK. 2001. Ultrastructural analysis of ventrolateral periaqueductal gray projections to the A7 catecholamine cell group. Neuroscience 104:181-197. Balcita-Pedicino JJ, Sesack SR. 2007. Orexin axons in the rat ventral tegmental area synapse infrequently onto dopamine and gamma-aminobutyric acid neurons. J Comp Neurol 503:668-684. Benarroch EE. 2001. Pain-autonomic interactions: a selective review. Clin Auton Res 11:343-349. Benarroch EE. 2008. Descending monoaminergic pain modulation: bidirectional control and clinical relevance. Neurology 71:217-221. Bernard JF, Besson JM. 1990. The spino(trigemino)pontoamygdaloid pathway: electrophysiological evidence for an involvement in pain processes. J Neurophysiol 63:473-490. Besson JM. 1999. The neurobiology of pain. Lancet 353:1610-1615. Clark FM, Proudfit HK. 1991a. The projection of noradrenergic neurons in the A7 catecholamine cell group to the spinal cord in the rat demonstrated by anterograde tracing combined with immunocytochemistry. Brain Res 547:279-288. Clark FM, Proudfit HK. 1991b. Projections of neurons in the ventromedial medulla to pontine catecholamine cell groups involved in the modulation of nociception. Brain Res 540:105-115. Cui M, Feng Y, McAdoo DJ, Willis WD. 1999. Periaqueductal gray stimulation-induced inhibition of nociceptive dorsal horn neurons in rats is associated with the release of norepinephrine, serotonin, and amino acids. J Pharmacol Exp Ther 289:868-876. Cutler DJ, Morris R, Sheridhar V, Wattam TA, Holmes S, Patel S, Arch JR, Wilson S, Buckingham RE, Evans ML, Leslie RA, Williams G. 1999. Differential distribution of orexin-A and orexin-B immunoreactivity in the rat brain and spinal cord. Peptides 20:1455-1470. Dahlstroem A, Fuxe K. 1964. Evidence for the Existence of Monoamine-Containing Neurons in the Central Nervous System. I. Demonstration of Monoamines in the Cell Bodies of Brain Stem Neurons. Acta Physiol Scand Suppl:SUPPL 232:231-255. Date Y, Mondal MS, Matsukura S, Nakazato M. 2000. Distribution of orexin-A and orexin-B (hypocretins) in the rat spinal cord. Neurosci Lett 288:87-90. de Lecea L, Kilduff TS, Peyron C, Gao X, Foye PE, Danielson PE, Fukuhara C, Battenberg EL, Gautvik VT, Bartlett FS, 2nd, Frankel WN, van den Pol AN, Bloom FE, Gautvik KM, Sutcliffe JG. 1998. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity. Proc Natl Acad Sci U S A 95:322-327. Dong HL, Fukuda S, Murata E, Zhu Z, Higuchi T. 2006. Orexins increase cortical acetylcholine release and electroencephalographic activation through orexin-1 receptor in the rat basal forebrain during isoflurane anesthesia. Anesthesiology 104:1023-1032. Ferguson AV, Samson WK. 2003. The orexin/hypocretin system: a critical regulator of neuroendocrine and autonomic function. Front Neuroendocrinol 24:141-150. Fields H. 2004. State-dependent opioid control of pain. Nat Rev Neurosci 5:565-575. Gauriau C, Bernard JF. 2002. Pain pathways and parabrachial circuits in the rat. Exp Physiol 87:251-258. Gray EG. 1959a. Electron microscopy of neuroglial fibrils of the cerebral cortex. J Biophys Biochem Cytol 6:121-122. Gray EG. 1959b. Electron microscopy of synaptic contacts on dendrite spines of the cerebral cortex. Nature 183:1592-1593. Haynes AC, Jackson B, Chapman H, Tadayyon M, Johns A, Porter RA, Arch JR. 2000. A selective orexin-1 receptor antagonist reduces food consumption in male and female rats. Regul Pept 96:45-51. Helmstetter FJ, Tershner SA, Poore LH, Bellgowan PS. 1998. Antinociception following opioid stimulation of the basolateral amygdala is expressed through the periaqueductal gray and rostral ventromedial medulla. Brain Res 779:104-118. Hervieu GJ, Cluderay JE, Harrison DC, Roberts JC, Leslie RA. 2001. Gene expression and protein distribution of the orexin-1 receptor in the rat brain and spinal cord. Neuroscience 103:777-797. Holden JE, Proudfit HK. 1998. Enkephalin neurons that project to the A7 catecholamine cell group are located in nuclei that modulate nociception: ventromedial medulla. Neuroscience 83:929-947. Holden JE, Schwartz EJ, Proudfit HK. 1999. Microinjection of morphine in the A7 catecholamine cell group produces opposing effects on nociception that are mediated by alpha1- and alpha2-adrenoceptors. Neuroscience 91:979-990. Horvath TL, Peyron C, Diano S, Ivanov A, Aston-Jones G, Kilduff TS, van Den Pol AN. 1999. Hypocretin (orexin) activation and synaptic innervation of the locus coeruleus noradrenergic system. J Comp Neurol 415:145-159. Howorth PW, Teschemacher AG, Pickering AE. 2009. Retrograde adenoviral vector targeting of nociresponsive pontospinal noradrenergic neurons in the rat in vivo. J Comp Neurol 512:141-157. Hughes DI, Mackie M, Nagy GG, Riddell JS, Maxwell DJ, Szabo G, Erdelyi F, Veress G, Szucs P, Antal M, Todd AJ. 2005. P boutons in lamina IX of the rodent spinal cord express high levels of glutamic acid decarboxylase-65 and originate from cells in deep medial dorsal horn. Proc Natl Acad Sci U S A 102:9038-9043. Hunt SP, Mantyh PW. 2001. The molecular dynamics of pain control. Nat Rev Neurosci 2:83-91. Iremonger KJ, Benediktsson AM, Bains JS. 2010. Glutamatergic synaptic transmission in neuroendocrine cells: Basic principles and mechanisms of plasticity. Front Neuroendocrinol 31:296-306. Jochem J. 2009. Orexin type 1 receptor antagonist SB 334867 inhibits the central histamine-induced resuscitating effect in rats subjected to haemorrhagic shock. Inflamm Res 58 Suppl 1:36-37. Joh TH, Hwang O. 1987. Dopamine beta-hydroxylase: biochemistry and molecular biology. Ann N Y Acad Sci 493:342-350. Kajiyama S, Kawamoto M, Shiraishi S, Gaus S, Matsunaga A, Suyama H, Yuge O. 2005. Spinal orexin-1 receptors mediate anti-hyperalgesic effects of intrathecally-administered orexins in diabetic neuropathic pain model rats. Brain Res 1044:76-86. Kaminski T, Smolinska N, Nitkiewicz A, Przala J. 2010. Expression of orexin receptors 1 (OX1R) and 2 (OX2R) in the porcine pituitary during the oestrous cycle. Anim Reprod Sci 117:111-118. Krout KE, Jansen AS, Loewy AD. 1998. Periaqueductal gray matter projection to the parabrachial nucleus in rat. J Comp Neurol 401:437-454. Lobo MV, Alonso FJ, Arenas MI, Caso E, Fraile B, del Rio RM. 2002. Ultrastructural staining with sodium metaperiodate and sodium borohydride. J Histochem Cytochem 50:11-19. Lu XY, Bagnol D, Burke S, Akil H, Watson SJ. 2000. Differential distribution and regulation of OX1 and OX2 orexin/hypocretin receptor messenger RNA in the brain upon fasting. Horm Behav 37:335-344. Manning BH, Mayer DJ. 1995. The central nucleus of the amygdala contributes to the production of morphine antinociception in the rat tail-flick test. J Neurosci 15:8199-8213. Marcus JN, Aschkenasi CJ, Lee CE, Chemelli RM, Saper CB, Yanagisawa M, Elmquist JK. 2001. Differential expression of orexin receptors 1 and 2 in the rat brain. J Comp Neurol 435:6-25. Martin WJ, Coffin PO, Attias E, Balinsky M, Tsou K, Walker JM. 1999. Anatomical basis for cannabinoid-induced antinociception as revealed by intracerebral microinjections. Brain Res 822:237-242. McMillan PN, Luftig RB. 1975. Preservation of membrane ultrastructure with aldehyde or imidate fixatives. J Ultrastruct Res 52:243-260. Min MY, Shih PY, Wu YW, Lu HW, Lee ML, Yang HW. 2009. Neurokinin 1 receptor activates transient receptor potential-like currents in noradrenergic A7 neurons in rats. Mol Cell Neurosci 42:56-65. Min MY, Wu YW, Shih PY, Lu HW, Lin CC, Wu Y, Li MJ, Yang HW. 2008. Physiological and morphological properties of, and effect of substance P on, neurons in the A7 catecholamine cell group in rats. Neuroscience 153:1020-1033. Moore RY, Bloom FE. 1978. Central catecholamine neuron systems: anatomy and physiology of the dopamine systems. Annu Rev Neurosci 1:129-169. Moore RY, Bloom FE. 1979. Central catecholamine neuron systems: anatomy and physiology of the norepinephrine and epinephrine systems. Annu Rev Neurosci 2:113-168. Nambu T, Sakurai T, Mizukami K, Hosoya Y, Yanagisawa M, Goto K. 1999. Distribution of orexin neurons in the adult rat brain. Brain Res 827:243-260. Nuseir K, Heidenreich BA, Proudfit HK. 1999. The antinociception produced by microinjection of a cholinergic agonist in the ventromedial medulla is mediated by noradrenergic neurons in the A7 catecholamine cell group. Brain Res 822:1-7. Nuseir K, Proudfit HK. 2000. Bidirectional modulation of nociception by GABA neurons in the dorsolateral pontine tegmentum that tonically inhibit spinally projecting noradrenergic A7 neurons. Neuroscience 96:773-783. Ohno K, Sakurai T. 2008. Orexin neuronal circuitry: role in the regulation of sleep and wakefulness. Front Neuroendocrinol 29:70-87. Palay SL. 1956a. Structure and function in the neuron. Prog Neurobiol 1:64-82. Palay SL. 1956b. Synapses in the central nervous system. J Biophys Biochem Cytol 2:193-202. Paxinos G, Watson C. 2005. The rat brain in stereotaxic coordinates, 5th ed. Amsterdam ; Boston: Elsevier Academic Press. Peters A, Hinds PL, Vaughn JE. 1971. Extent of stain penetration in sections prepared for electron microscopy. J Ultrastruct Res 36:37-45. Peters A, Palay SL, Webster Hd. 1991. The fine structure of the nervous system : neurons and their supporting cells, 3rd ed. New York: Oxford University Press. Puskas N, Papp RS, Gallatz K, Palkovits M. 2010. Interactions between orexin-immunoreactive fibers and adrenaline or noradrenaline-expressing neurons of the lower brainstem in rats and mice. Peptides 31:1589-1597. Rasmussen K, Hsu MA, Yang Y. 2007. The orexin-1 receptor antagonist SB-334867 blocks the effects of antipsychotics on the activity of A9 and A10 dopamine neurons: implications for antipsychotic therapy. Neuropsychopharmacology 32:786-792. Reynolds ES. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208-212. Sabatini DD, Bensch K, Barrnett RJ. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol 17:19-58. Sakurai T. 2007. [Regulatory mechanism of sleep/wakefulness states by orexin]. Tanpakushitsu Kakusan Koso 52:1840-1848. Sakurai T, Amemiya A, Ishii M, Matsuzaki I, Chemelli RM, Tanaka H, Williams SC, Richarson JA, Kozlowski GP, Wilson S, Arch JR, Buckingham RE, Haynes AC, Carr SA, Annan RS, McNulty DE, Liu WS, Terrett JA, Elshourbagy NA, Bergsma DJ, Yanagisawa M. 1998. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92:1 page following 696. Taheri S, Mahmoodi M, Opacka-Juffry J, Ghatei MA, Bloom SR. 1999. Distribution and quantification of immunoreactive orexin A in rat tissues. FEBS Lett 457:157-161. Tershner SA, Helmstetter FJ. 2000. Antinociception produced by mu opioid receptor activation in the amygdala is partly dependent on activation of mu opioid and neurotensin receptors in the ventral periaqueductal gray. Brain Res 865:17-26. Tose R, Kushikata T, Yoshida H, Kudo M, Furukawa K, Ueno S, Hirota K. 2009. Interaction between orexinergic neurons and NMDA receptors in the control of locus coeruleus-cerebrocortical noradrenergic activity of the rat. Brain Res 1250:81-87. Watson ML. 1958. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475-478. Watson SL, Watson CJ, Baghdoyan HA, Lydic R. 2010. Thermal nociception is decreased by hypocretin-1 and an adenosine A1 receptor agonist microinjected into the pontine reticular formation of Sprague Dawley rat. J Pain 11:535-544. Willie JT, Chemelli RM, Sinton CM, Yanagisawa M. 2001. To eat or to sleep? Orexin in the regulation of feeding and wakefulness. Annu Rev Neurosci 24:429-458. Yeomans DC, Proudfit HK. 1990. Projections of substance P-immunoreactive neurons located in the ventromedial medulla to the A7 noradrenergic nucleus of the rat demonstrated using retrograde tracing combined with immunocytochemistry. Brain Res 532:329-332. Yeomans DC, Proudfit HK. 1992. Antinociception induced by microinjection of substance P into the A7 catecholamine cell group in the rat. Neuroscience 49:681-691. Záborszky L, Wouterlood FG, Lanciego JL, Heimer L. 2006. Neuroanatomical tract-tracing 3 : molecules, neurons, and systems, [3rd ed. New York: Springer. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47300 | - |
dc.description.abstract | 食慾蛋白(orexin)為下視丘分泌的神經蛋白,它參與調節許多的腦部功能,其中包括痛覺的抑制。 有研究指出,A7的兒茶酚胺神經纖維投射到脊髓背角處並且調節脊髓的止痛機制,而食慾蛋白A對於A7的兒茶酚胺神經有興奮的效果。然而,與A7的兒茶酚胺神經細胞相關的食慾蛋白A神經末梢之型態沒有詳細的被描述。因此,本實驗使用小鼠抗酪氨酸羥化酶作為一級抗體標定A7的兒茶酚胺神經細胞,二級抗體使用結合直徑一奈米金粒子的抗小鼠免疫球蛋白,再用銀粒子增加金粒子的直徑,使標定結果可以直接在穿透式電子顯微鏡下被觀察。為了分辨與A7兒茶酚胺神經細胞不同,一級抗體使用兔抗食慾蛋白A來標定有食慾蛋白A的神經纖維。即可在光學顯微鏡下觀察到大量黑色的食慾蛋白A神經纖維在位於三叉神經核區兩百微米前方的A7核區,並且和由酪氨酸羥化酶標定A7兒茶酚胺神經細胞的細胞本體或樹突形成直接的連繫。在電子顯微鏡下,這些接觸是有功能的突觸,在特定區域有突觸前和突觸後細胞膜以及清晰可見的突觸間隙,有增厚的突觸後膜,還有突觸小泡聚集在突觸前側。這些結果顯示食慾蛋白A可經由調控下行的正腎上腺素系統來止痛。 | zh_TW |
dc.description.abstract | Orexin is a hypothalamic neuropeptide that is involved in many brain functions, including neuropathic pain modulation. Our previous results have shown that orexin-A has excitatory effect on noradrenergic (NAergic) neurons of A7 catecholamine cell groups, which projects NAergic fibers to the dorsal spinal cord and plays a role in spinal antinociception. However morphological detail of that orexin-A terminals associated with NAergic A7 neurons has not been described. Here, we use mouse anti-tyrosine hydroxylase (TH) to identify NAergic neurons in A7 area, and emerge by 1nm gold conjugated anti-mouse IgG, and then intensify by silver enhancement. To distinguish orexin- immunoreactive (ir) fibers from NAergic neurons, we use rabbit anti-orexin-A to identify orexin containing fibers. At light microscopic level, there were many of orexin-ir buttons which were stained in black and found to make physical contacts on the dendrites and soma of A7 NAergic neurons, identified as their immunostaining profile of showing TH-ir and location of ~ 200 micrometers rostral to the trigeminal motor nucleus. Under electron microscopic observation, these contacts were confirmed to be functional synaptic connections as a restricted zone of parallel pre- and postsynaptic membrane specializations with visible synaptic cleft, and/or associated postsynaptic thickening, and the accumulation of synaptic vesicles in the presynaptic profile were identified. These results suggest that orexin-A might exert analgesia through modulation of descending NAergic system. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:54:07Z (GMT). No. of bitstreams: 1 ntu-99-R97b41029-1.pdf: 2875482 bytes, checksum: 26aa6c11f8d51f53402d2afc6216da70 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
致謝 i Abstract ii 中文摘要 iv 目錄 v 緒論 1 A7 兒茶酚胺細胞群的生理和形態上的功能 1 疼痛 2 下行痛覺調控機制(Descending pain modulation) 3 食慾蛋白神經細胞的生理和形態上的功能 4 實驗目標 5 實驗材料和方法 6 灌流和固定 6 切片 6 單一標定免疫金銀染色法(immunogold-silver method) 7 單一標定免疫過氧化酶染色法(immunoperoxidase method) 7 包埋前雙標定染色 8 穿透式電子顯微鏡的組織處理 8 超微結構的定義 9 結果 10 定義A7兒茶酚胺細胞群的正腎上腺素神經細胞在腦幹的位置 10 食慾蛋白神經纖維在A7核區的光學顯微鏡下之特徵 10 食慾蛋白神經纖維在A7核區的超微結構特徵 10 食慾蛋白神經纖維在A7核區與正腎上腺素細胞的關係 11 各種類突觸的數量 12 討論 13 結論 13 食慾蛋白和A7正腎上腺素系統的關係 13 技術上的改進 14 References 15 圖表 23 圖一:A7核區正腎上腺素細胞群的定位 24 圖二:食慾蛋白神經纖維延伸到A7核區和正腎上腺素細胞有接觸,可能有形成突觸 26 圖三:食慾蛋白的varicosities和A7核區中非正腎上腺素細胞的樹突形成突觸 27 圖四:食慾蛋白軸突末端和A7核區的非正腎上腺素細胞形成不對稱突觸 28 圖五:食慾蛋白軸突末端和A7核區的非正腎上腺素細胞形成不對稱突觸 29 圖六:食慾蛋白軸突末端和A7核區的正腎上腺素細胞形成不對稱突觸 31 表一:食慾蛋白軸突在A7核區之各突觸種類的影響力 32 | |
dc.language.iso | zh-TW | |
dc.title | 食慾蛋白神經軸突末端在A7核區的超微結構 | zh_TW |
dc.title | Ultrastructure of orexin releasing axonal terminals on Noradrenergic A7 neurons | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊琇雯(Hsiu-Wen Yang),陳瑞芬(Ruei-Feng Chen),傅毓秀(Yu-Show Fu) | |
dc.subject.keyword | 腦幹,A7 核區,食慾蛋白,下視丘分泌素,超微結構,止痛, | zh_TW |
dc.subject.keyword | brainstem,A7 cell group,orexin,hypocretin,ultrastructure,antinociception, | en |
dc.relation.page | 32 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 2.81 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。