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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳羿貞 | |
dc.contributor.author | Tai-Yu Shen | en |
dc.contributor.author | 沈泰宇 | zh_TW |
dc.date.accessioned | 2021-06-15T05:50:16Z | - |
dc.date.available | 2010-09-09 | |
dc.date.copyright | 2010-09-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-18 | |
dc.identifier.citation | Amler MH. 1969. The time sequence of tissue regeneration in human extraction wounds. Oral Surg Oral Med Oral Pathol 27: 309-318.
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Matsunaga T, Yanagiguchi K, Yamada S, Ohara N, Ikeda T, Hayashi Y. 2006. Chitosan monomer promotes tissue regeneration on dental pulp wounds. Journal of Biomedical Materials Research Part A 76: 711-720. Mayer T, Basdra EK, Komposch G, Staehle HJ. 1994. Localized alveolar ridge augmentation before orthodontic treatment. A case report. Int J Oral Maxillofac Surg 23: 226-228. Muzzarelli RA, G b, M B, L S, C C, G F. 1993. osteoconduction exerted by methylpyrrolidinone chitosan used in dental surgery. Biomaterials 14: 39-43. Muzzarelli RA, MM b, C T, G b, G F, MA B, M F, R G, P I. 1994. stimulatory effect on bone formation exerted by a modified chitosan. Biomaterials 15: 1075-1081. Nabers CL, Reed OM, Hamner JE. 1972. Gross and histologic evaluation of an autogenous bone graft 57 months postoperatively. . Journal of Periodontology 43: 702-704. Nielsen IM, Ellegaard B, Karring T. 1980. Kielbone® in healing interradicular lesions in monkeys. Journal of Periodontal Research 15: 328-337. P.R. K, L V, EB K, GW B. 1996. osteogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol 67: 1170-1175. Rao SB, Sharma CP. 1997. use of chitosan as a biomaterial: studies on its safety and hemostatic potential. journal of Biomedical Materials Research 34: 21. Schallhorn RG. 1967. eradication of bifurcation defects utilizing frozen autogenous hip marrow implants. Periodontal abstracts 15: 101-105. Serino G, Rao W, Iezzi G, Piattelli A. 2008. Polylactide and polyglycolide sponge used in human extraction sockets: bone formation following 3 months after its application. Clin Oral Implants Res 19: 26-31. Shi B, Zhou Y, Wang YN, Cheng XR. 2007. Alveolar ridge preservation prior to implant placement with surgical-grade calcium sulfate and platelet-rich plasma: a pilot study in a canine model. Int J Oral Maxillofac Implants 22: 656-665. Tiefengraber J, Diedrich P, Fritz U, Lantos P. 2002. Orthodontic space closure in combination with membrane supported healing of extraction sockets (MHE) a pilot study. J Orofac Orthop 63: 422-428. 方紹威. 1990. 幾丁質及幾丁聚糖在廢水處理,生化,食品和醫藥上之研究發展現況. 藥物食品檢驗局調查研究年報 8: 20-30. 巫冠誼. 2009. 以動物實驗探討不同方向扭力對迷你骨釘卸除扭力的影響台灣大學. 林佳妏等. 2001. 幾丁聚糖於生醫材料之應用與特性. 化工 48: 84-91. 林淑君等. 2006. 幾丁聚糖在牙周組織工程的應用. 中華牙周醫誌 11: 87-96. 柯天來等. 2002. 幾丁聚糖:新的牙周組織工程材料. 中華牙周醫誌 7: 109-118. 陳源厚. 2005. 評估矯正治療中運用迷你骨釘穩定性及危險性台灣大學. 陳瑞雲, 董醒任. 1999. 可吸收阻隔膜於引導骨再生的應用. Pages 79-95. 中華牙周醫誌. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47189 | - |
dc.description.abstract | 近年來由於齒顎矯正治療日益普及,成年矯正病患的人數比例也隨之增加,不過成年病患在尋求矯正治療的同時,往往合併有牙周破壞的狀況,讓矯正醫師在進行牙齒移動時遭受到更大的挑戰。臨床上引導組織再生的技術已十分成熟,也有許多材料被證實可以有效恢復齒槽嵴的高度與寬度,便利矯正治療時牙齒的移動或補綴物的製作。本研究即在探討微觀下葡萄糖胺單體是否具有骨誘導的能力?能否促成骨髓間葉細胞的分化?能否增進拔牙後齒槽骨的癒合?以及觀察齒槽修復時骨生成的進行機制。本實驗以兩隻小獵犬為研究對象,實驗設計如下:(1)dog A下顎拔除第一至第四小臼齒,在第二及第三小臼齒近心齒槽窩填入葡萄糖胺及骨髓混合物為實驗組I,在第二及第三小臼齒遠心齒槽窩僅填入葡萄糖胺為實驗組II,第一小臼齒及第四小臼齒近心齒槽窩則不填入材料當成對照組,實驗期間為六個月;(2)dog A及B的上顎拔除第一至第三小臼齒,在第二小臼齒近心齒槽窩填入葡萄糖胺及骨髓混合物為實驗組I,在第二小臼齒遠心齒槽窩僅填入葡萄糖胺為實驗組II,第三小臼齒近心齒槽窩則不填入材料當成對照組,實驗期間四個月;(3)dog B的下顎實驗設計與dog A下顎相同,但實驗期間則為一個月。實驗期間每隔三星期實施骨頭標定。動物犧牲後將齒槽窩及其周圍骨頭樣本取下,以顯微鏡觀察齒槽骨癒合情形。實驗結果發現:(1)單純充填葡萄糖胺的齒槽骨修復速度比充填葡萄糖胺及骨髓混合物的齒槽骨修復速度慢;(2)有充填材料的實驗組其形成的骨小樑寬度皆比未充填材料的對照組寬;(3)齒槽骨的修復是由外圍至中心,由根尖部至牙冠部;(4)實驗組與對照組的長期觀察結果並無顯著差異,皆形成成熟之骨組織。 | zh_TW |
dc.description.abstract | In recent years, the number of adult patients seeking orthodontic treatment has increased significantly, therefore the orthodontists face challenges in moving the dentition with compromised periodontal support. The orthodontic tooth movement could be facilitated if the alveolar ridge is preserved after tooth extraction. The techniques of guided tissue regeneration have been extensively used in periodontal regeneration. Many biomaterials can provide necessary mechanical support and cell adhesion for bone tissue regeneration, whereas they cannot guide cellular phenotype as growth factors can. Chitosan, a natural polysaccharide biomaterial, exhibits nontoxicity, biodegradability, biocompatibility, and antibacterial properties. The aim of this animal study was to assess the effect of glucosamine, i.e. chitosan monomer, on alveolar bone healing of extraction socket. The study was conducted on the teeth (p1~p4 in mandible and p1~p3 in maxilla) of 2 Mongrel dogs in a split-mouth design. Immediately after tooth extraction, the mesial sockets of the second and third mandibular premolars and the second maxillary premolar were filled with the mixture of glucosamine powder and bone marrow aspirate from femur (experiment group I). The distal sockets of the second and third mandibular premolars and the second maxillary premolar were filled with glucosamine powder only (experiment group II). For each group, the experimental procedures were dome at different time for histomorphometric examination at 3 observation time-point of 30, 120, and 180 days. The extraction sockets at contra-lateral side without glucosamine fillings served as a control group. Moreover, the process of bone repair in extraction socket was examined by using the technique of fluorochrome sequential labeling. After the animals were sacrificed, the specimens were processed for examination by fluorescence microscopy and for paraffin sections and stained with haematoxylin-eosin. The results showed: (1) In the experimental sockets, especially filled with glucosamine and bone marrow aspirate (group I), the signs of bone formation were obvious and the number of proliferating cells were higher than that in control group. (2) The size of bone trabeculae in experimental sockets was larger compared to the control group. (3) Both the histological examinations and the observations via fluorochrome labeling revealed that the alveolar sockets heal from peripheral to the central and from apical to the coronal part of the socket. (4) All the sockets healed completely, and no significant difference was noted among the experimental and control groups after an observation period of 180 days. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:50:16Z (GMT). No. of bitstreams: 1 ntu-99-R96422023-1.pdf: 1946431 bytes, checksum: a38fb2170a1c4b74133a611950723d2d (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 摘要………………………………………………… ………………………………… i
Abstract…………… …………………………………………………………… ……ii 目錄……………………………………………………… ……………………………...I 圖目錄…………………………………………………………………………… …...III 表目錄………………………………………………………………………….…… ..IV 第一章 文獻回顧……………………………………………………………………….1 1. 拔牙後之齒槽變化………………………………………………………………..1 1-1 齒槽內的骨生成……………………………………………………………..1 1-2 齒槽嵴形態的改變…………………………………………………………..2 2. 齒槽嵴保存術……………………………………………………………………..2 2-1 齒槽嵴保存術的原理………………………………………………………..3 2-2 應用於齒槽嵴保存術的移植物……………………………………………..3 2-2-1 自體移植物…………………………………………………………...4 2-2-2 異體移植物…………………………………………………………...5 2-2-3 異種移植物…………………………………………………………...5 2-2-4 異質移植物…………………………………………………………...5 3. 葡萄糖胺及其衍生物……………………………………………………………..6 3-1 葡萄糖胺及其衍生物之來源與結構..............................................................6 3-2 葡萄糖胺及其衍生物之工業應用..................................................................6 3-3 葡萄糖胺及其衍生物之生醫材料應用……………………………………..7 3-4 葡萄糖胺及其衍生物之牙科應用…………………………………………..7 第二章 實驗目的……………………………………………………………………….9 第三章 實驗材料與方法……………………………………………………………...10 1. 實驗設計………...……………………………………………………………...10 2. 實驗過程……………………………………………………………………...11 2-1 動物麻醉……………………………………………………………...11 2-2 骨髓抽取與材料混合…………………………...……………………12 2-3 牙齒拔除及填充材料…………………………...……………………12 2-4 臨床檢查……………………………………………………………...13 2-5 骨頭標定……………………………………………………………...13 2-6 犧牲過程……………………………………………………………...14 2-7 放射線學檢查………………………………………………………...14 2-8 組織切片的製作與檢查……………………………………………...15 2-9 統計學分析…………………………………………………………...16 第四章 實驗結果……………………………………………………………………...17 1. 拔牙後一個月齒槽骨生成之組織學觀察……………………………………..17 1-1 實驗組I(填充葡萄糖胺及骨髓)一個月之組織學觀察…………………17 1-1-1 Dog B右下第二小臼齒之近心齒槽……………………………….17 1-1-2 Dog B右下第三小臼齒之近心齒槽……………………………….18 1-2 實驗組II(僅填充葡萄糖胺)一個月之組織學觀察……………...………20 1-2-1 Dog B右下第二小臼齒之遠心齒槽……………………………….20 1-2-2 Dog B右下第三小臼齒之遠心齒槽……………………………….21 1-3 對照組一個月之組織學觀察……………………………………………..23 1-4 拔牙後一個月組別之骨小樑面積計算…………………………………..24 2. 錐狀電腦斷層影像重組之數值分析…………………………………………..24 2-1拔牙後一個月組別之齒槽密度與體積計算… ………………… ……24 2-2 拔牙後六個月組別之齒槽嵴高度與寬度計算…………………… ……25 3. 拔牙後四個月齒槽骨生成之觀察……………………………………………..26 3-1 臨床及放射線學觀察…………………..……..…………………………..26 3-2組織學觀察………………………….…..……..…………………………..27 4. 拔牙後六個月齒槽骨生成之組織學觀察.…..……..……………………….....28 4-1 臨床及放射線學觀察…………………..……..…………………………..28 4-2組織學觀察………………………….…..……..…………………………..29 5. 拔牙後齒槽骨生成之未脫鈣磨片螢光觀察..……..…………………………..30 5-1 拔牙後一個月之組別……………….…..……..……………………….....31 5-2 拔牙後四個月之組別……………….…..……..……………………….....32 5-3 拔牙後六個月之組別……………….…..……..……………………….....33 第五章 討論…………………………………………………………………………...34 1. 動物實驗模型的選擇……………………………………………………...…...34 2 實驗設計探討…………………………………………………………………...34 2-1 皮瓣縫合之選擇………………………………….………………………...34 2-2 實驗觀察時間的選擇…………………………….………………………...35 2-3 H&E染色片之處理……………………………….………………………...35 3. 短期觀察組別之組織學表現………………………….………………..……...36 4. 長期觀察組別之組織學表現………………………….………………..……...37 5. 螢光磨片之觀察………………………………………………...……………...39 第六章 結論…………………………………………………………………………...40 第七章 未來展望 ……………………………………………………………………41 參考文獻…………………………………………………………………………….....42 | |
dc.language.iso | zh-TW | |
dc.title | 葡萄糖胺對拔牙後齒槽修復之影響:動物實驗評估 | zh_TW |
dc.title | Effect of glucosamine on alveolar bone repair following tooth extraction: evaluation with an animal model | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 章浩宏,呂炫? | |
dc.subject.keyword | 葡萄糖胺,齒槽嵴,保存,動物實驗, | zh_TW |
dc.subject.keyword | glucosamine,alveolar ridge preservation,animal model, | en |
dc.relation.page | 45 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-19 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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