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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊平世 | |
dc.contributor.author | Jui-Fan Hsieh | en |
dc.contributor.author | 謝瑞帆 | zh_TW |
dc.date.accessioned | 2021-06-15T05:45:58Z | - |
dc.date.available | 2015-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-18 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47043 | - |
dc.description.abstract | 麗叩甲屬 (Campsosternus Latreille, 1834)是少數帶有金屬光澤之叩頭蟲種類,全屬約 80 種,主要分布於東洋區 (Oriental realm),唯一例外種 C. papuensis Van Zwaluwenburg, 1931 分布於澳洲區 (Australian realm) 的巴布亞新幾內亞 (Paupa New Guinea)。往昔文獻一共記錄 5 種麗叩頭蟲分佈於臺灣島內,分別為大青叩頭蟲 (C. auratus (Drury, 1773))、臺灣大青叩頭蟲 (C. guishuni Ohira, 1977)、大綠叩頭蟲 (C. mirabilis Fleutiaux, 1930)、虹彩叩頭蟲 (C. watanabei Miwa, 1927) 以及紅緣大青叩頭蟲 (C. yasuakii Suzuki, 2002)。鑑定特徵主要依據成蟲之體型大小、體色、斑紋、觸角長寬比和比對雄蟲外部生殖器。然而此屬叩頭蟲體色及斑紋之變異極大,鑑定上相當困難。因此,本研究利用分子標記針對臺灣產麗叩甲屬進行親緣關係之研究;研究結果顯示粒線體 DNA COI 與 16S rDNA 皆能有效鑑別臺灣產麗叩甲之物種,確認中國產之朱肩麗叩頭蟲 (C. gemma Candèze, 1857)、虹彩叩頭蟲及紅緣大青叩頭蟲等為 3 個獨立的分類單元。大綠叩頭蟲與紅緣大青叩頭蟲則無法區分為 2 個獨立種,應處理為同物異名。臺灣大青叩頭蟲,至今仍只有一隻雌蟲的模示標本,由於沒有分子資料且外部形態比較也缺乏樣本,因此需要更多線索才能證實此種是否為大綠叩頭蟲之變種。 | zh_TW |
dc.description.abstract | The species in genus Campsosternus Latreille, 1834 are few of the click beetles which have metallic colors. This genus contains about 80 species mostly from Oriental realm, with an exceptional species: C. papuensis Van Zwaluwenburg, 1931, distributed in Papau New Guinea in Australian realm. According to references, 5 species was recorded in Taiwan: C. auratus (Drury, 1773), C. guishuni Ohira, 1977, C. mirabilis Fleutiaux, 1930, C. watanabei Miwa, 1927, and C. yasuakii Suzuki, 2002. The diagnosis is based on adult morphology: body size, body color, maculation, antennal length-width ratio, and compare the male genitalia. However, the species is difficult to identify due to the highly variable body color and maculation. Therefore, a molecular phylogeny study of Campsosternus in Taiwan was conducted in this study by molecular marker. The results of mitochondrial DNA COI and 16S rDNA show excellent resolution on species level. Both genes support that C. gemma Candèze, 1857, C. watanabei, and C. yasuakii are three independent taxa. C. mirabilis and C. yasuakii cannot be separate from each other and should be treated as synonym. C. guishuni has only one collection female type specimen as representative. It needs more evidence to check whether C. guishuni is a variant of C. mirabilis or not. | en |
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dc.description.tableofcontents | 目錄
口試委員會審定書……………………………………………………………….…....i 致謝................................................................................................................................ii 中文摘要……………………………………………………………………………...iii 英文摘要.......................................................................................................................iv 第一章 前言………...……………………………………………………….............1 第二章 往昔研究………………………………………………………....................4 2.1 傳統分類…………………………………………………………………...4 2.1.1 叩頭蟲科高階分類…………………………………………………4 2.1.2 麗叩甲屬與梳角叩甲屬之關係……………………………………5 2.1.3 麗叩甲屬之分類學研究……………………………………………5 2.2 分子標記…………………………………………………………………...6 第三章 材料與方法…………………………………………………………………8 3.1 標本來源…………………………………………………………...………8 3.2 標本採集……………………………………………………………..…….8 3.3 標本製作與保存…………………………………………………………...8 3.3.1 浸液標本 (分子資料建立前)……………………………………...8 3.3.2 乾燥標本 (分子資料建立後)……………………………………...9 3.4 形態描述…………………………………………………………………...9 3.5 形態測量……………………………………………………………….....12 3.6 研究使用之樣本……………………………………………………….....12 3.7 DNA序列分析…………………………………………………………....13 3.7.1 叩頭蟲 DNA 之萃取………………………………………….....13 3.7.2 DNA 增幅引子…………………………………………………...13 3.7.3 序列處理………………………………………………………......14 3.7.4 核酸取代模示之選取…………………………………………......14 3.7.5 分子系統發生樹之重建………………………………………......14 第四章 結果……………………………………………………………….………15 4.1 親緣關係………………………………………………….………………15 4.1.1 序列分析……………………………………………….………….15 4.1.2 分子樹………………………………………………….………….15 4.1.3 遺傳距離……………………………………………….………….16 4.2 分類學….…………………………………………………………………18 第五章 討論………………………………………………………………………..37 5.1 序列分析比較……………………………………………………….……37 5.2 親緣關係…………………………………………………………….……37 5.2.1 粒線體 COI 親緣關係樹之探討…………………………….......37 5.2.2 粒線體 16S 親緣關係樹之探討…………………………………38 5.2.3 粒線體 COI + 16S 親緣關係樹之探討……………………….…39 5.3 分類問題與保育名錄之修訂………………………………….................39 引用文獻……………………………………………………………………………..41 附錄 表目錄 表一 麗叩頭蟲樣本………………………………………………………………..47 表二 探討麗叩頭蟲屬之分子標記序列核苷酸組成...……………………..…….50 表三 粒線體 COI 序列之 Kishino-Hasegawa檢驗.…………………………….51 表四 粒線體 16S rDNA 序列之 Kishino-Hasegawa檢驗.……………………...52 表五 粒線體 COI + 16S 序列之 Kishino-Hasegawa 檢驗.…………………….53 表六 臺灣產 4 種麗叩頭蟲評分結果及建議保育等級.………………………...54 圖目錄 圖一 尖鞘叩頭蟲亞科下之族、屬級成員。………………………………………55 圖二 前胸背板帶有鋸齒狀邊緣。………………………………………..………..55 圖三 翅鞘末端呈尖刺狀。….…………………………………………………….55 圖四 大青叩頭蟲幼蟲之觸角。………………………………………………..…..56 圖五 大青叩頭蟲幼蟲第二節觸角之感覺孔群。…………………………………56 圖六 叩頭蟲背側 (Campsosternus auratus)。……………………………………57 圖七 叩頭蟲腹側 (Campsosternus auratus)。……………………………………58 圖八 雄性外生殖器 (Campsosternus mirabilis)。………………………………..59 圖九 雌性外生殖器 (Campsosternus mirabilis)。………………………………..59 圖十 交尾囊 (Campsosternus mirabilis)。………………………………………..60 圖十一 交尾囊內骨片分佈 (Campsosternus yasuakii)。…………………………60 圖十二 交尾囊內骨片 (Campsosternus yasuakii)。………………………………61 圖十三 交尾囊內骨片 (Campsosternus yasuakii)。………………………………61 圖十四 麗叩頭蟲屬粒線體 COI 序列核苷酸替換之轉換 (s) 及顛換 (v) 飽和度測試。…………………………………………………………………………62 圖十五 麗叩頭蟲屬粒線體 16S 序列核苷酸替換之轉換 (s) 及顛換 (v) 飽和度測試。…………………………………………………………………………63 圖十六 粒線體 COI 序列建立最大似然樹,並進行 1000 次bootstrap 檢測。………………………………………………………………………………64 圖十七 粒線體 COI 序列建立貝氏系統樹,運算 2,000,000 個世代。………………………………………………………………………………65 圖十八 粒線體 16S 序列建立最大似然樹,並進行 1000 次bootstrap 檢測。………………………………………………………………………………66 圖十九 粒線體 16S 序列建立貝氏系統樹,運算 2,000,000 個世代。………………………………………………………………………………67 圖二十 粒線體 COI + 16S 序列建立最大似然樹,並進行 1000 次bootstrap 檢測。………………………………………………………………………………68 圖廿一 粒線體 COI +16S 序列建立貝氏系統樹,運算 2,000,000 個世代。………………………………………………………………………………69 圖廿二 大青叩頭蟲 (Campsosternus auratus) 暗綠色型。……………………...70 圖廿三 大青叩頭蟲 (Campsosternus auratus) 藍綠色型。……………………..71 圖廿四 大青叩頭蟲 (Campsosternus auratus) 銅綠色型。……………………...72 圖廿五 小楯片成六角形 (Campsosternus auratus)。…………………………….73 圖廿六 小楯片成橢圓形 (Campsosternus auratus)。…………………………….73 圖廿七 虹彩叩頭蟲 (Campsosternus watanabei)。……………………………….74 圖廿八 紅緣大青叩頭蟲 (Campsosternus yasuakii)。……………………………75 圖廿九 臺灣大青叩頭蟲 (Ohira 私人收藏)。……………………………………76 圖三十 大綠叩頭蟲 (Campsosternus mirabilis) 深綠色型。…………………….77 圖卅一 大綠叩頭蟲 (Campsosternus mirabilis) 藍綠色型。…………………….78 圖卅二 大綠叩頭蟲 (Campsosternus mirabilis) 深紫色型。…………………….79 圖卅三 大綠叩頭蟲 (Campsosternus mirabilis) 有斑型。………………………80 圖卅四 小楯片呈倒五角形 (Campsosternus watanabei)。………………………81 圖卅五 小楯片呈五角形 (Campsosternus yasuakii)。……………………………81 圖卅六 交尾囊內骨片 (Campsosternus mirabilis)。…………………………..…82 圖卅七 前側骨片 (Campsosternus mirabilis)。…………………………………..82 圖卅八 交尾囊內骨片 (Campsosternus watanebei)。…………………………….83 圖卅九 前側骨片 (Campsosternus watanebei)。………………………………….83 圖四十 雄性外生殖器 (Campsosternus auratus)。……………………………….84 圖四十一 雌性外生殖器 (Campsosternus auratus)。…………………………….84 圖四十二 雌性交尾囊內骨片 (Campsosternus auratus)。……………………….85 圖四十三 交尾囊內骨片 (Campsosternus auratus)。…………………………….86 圖四十四 前側骨片(Campsosternus auratus)。…………………………………..86 圖四十五 大青叩頭蟲 (Campsosternus auratus) 分布圖。……………………..87 圖四十六 臺灣大青叩頭蟲 (Campsosternus guishuni) 分布圖。…………..……88 圖四十七 雄性外生殖器 (Campsosternus mirabilis)。…………………………...89 圖四十八 雌性外生殖器 (Campsosternus mirabilis)。…………………………..89 圖四十九 小麗叩頭蟲 (Campsosternus dohrni ) 有斑型。………………………90 圖五十 小麗叩頭蟲 (Campsosternus dohrni var. fairmairai) 無斑型。………….91 圖五十一 大綠叩頭蟲 (Campsosternus mirabilis) 無斑型分布圖。………..….92 圖五十二 大綠叩頭蟲 (Campsosternus mirabilis) 分布圖。……………….……93 圖五十三 交尾囊內骨片 (Campsosternus gemma)。…………………….………94 圖五十四 前側骨片 (Campsosternus gemma)。……………………………….…94 圖五十五 雄性外生殖器 (Campsosternus watanabei)。……………….…………95 圖五十六 雌性外生殖器 (Campsosternus watanabei)。………………….………95 圖五十七 雌性交尾囊內骨片 (Campsosternus watanabei)。……………….……96 圖五十八 虹彩叩頭蟲 (Campsosternus watanabei) 分布圖。………………..…97 附錄 附錄一 COI 序列………………………………………………………………….98 附錄二 16S rDNA 序列………………………………………………………….101 附錄三 28S rDNA 序列………………………………………………………….104 附錄四 遺傳距離…………………………………………………………………106 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣產麗叩甲屬之親緣關係研究 | zh_TW |
dc.title | Phylogeny of the genus Campsosternus Latreille (Coleoptera: Elateridae) in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 柯俊成 | |
dc.contributor.oralexamcommittee | 李奇峰,鄭明倫 | |
dc.subject.keyword | 臺灣,麗叩甲屬,分子親緣關係,虹彩叩頭蟲, | zh_TW |
dc.subject.keyword | Taiwan,Campsosternus,molecular phylogeny,mitochondrion,COI, | en |
dc.relation.page | 108 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
顯示於系所單位: | 昆蟲學系 |
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