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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林中天 | |
dc.contributor.author | Yi-Jyun Lin | en |
dc.contributor.author | 林怡君 | zh_TW |
dc.date.accessioned | 2021-06-15T05:44:24Z | - |
dc.date.available | 2010-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-19 | |
dc.identifier.citation | 1. Agrawal VB, and Tsai RJ. Corneal epithelial wound healing. Indian journal of ophthalmology 51: 5-15, 2003.
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Suzuki K, Saito J, Yanai R, Yamada N, Chikama T, Seki K, and Nishida T. Cell-matrix and cell-cell interactions during corneal epithelial wound healing. Progress in retinal and eye research 22: 113-133, 2003. 38. Wakuta M, Morishige N, Chikama T, Seki K, Nagano T, and Nishida T. Delayed wound closure and phenotypic changes in corneal epithelium of the spontaneously diabetic Goto-Kakizaki rat. Investigative ophthalmology & visual science 48: 590-596, 2007. 39. Watson SL, Secker GA, and Daniels JT. The effect of therapeutic human serum drops on corneal stromal wound-healing activity. Current eye research 33: 641-652, 2008. 40. Xu KP, Li Y, Ljubimov AV, and Yu FS. High glucose suppresses epidermal growth factor receptor/phosphatidylinositol 3-kinase/Akt signaling pathway and attenuates corneal epithelial wound healing. Diabetes 58: 1077-1085, 2009. 41. Yamada C, King KE, and Ness PM. Autologous serum eyedrops: literature review and implications for transfusion medicine specialists. Transfusion 48: 1245-1255, 2008. 42. Yamada N, Yanai R, Kawamoto K, Nagano T, Nakamura M, Inui M, and Nishida T. Promotion of corneal epithelial wound healing by a tetrapeptide (SSSR) derived from IGF-1. Investigative ophthalmology & visual science 47: 3286-3292, 2006. 43. Yoon KC, Im SK, and Seo MS. Changes of tear film and ocular surface in diabetes mellitus. Korean journal of ophthalmology : KJO 18: 168-174, 2004. 44. Yoon KC, Im SK, Park YG, Jung YD, Yang SY, and Choi J. Application of umbilical cord serum eyedrops for the treatment of dry eye syndrome. Cornea 25: 268-272, 2006. 45. Yoon KC, You IC, Im SK, Jeong TS, Park YG, and Choi J. Application of umbilical cord serum eyedrops for the treatment of neurotrophic keratitis. Ophthalmology 114: 1637-1642, 2007. 46. Yu FS, Yin J, Xu K, and Huang J. Growth factors and corneal epithelial wound healing. Brain research bulletin 81: 229-235, 2010. 47. Zagon IS, Jenkins JB, Sassani JW, Wylie JD, Ruth TB, Fry JL, Lang CM, and McLaughlin PJ. Naltrexone, an opioid antagonist, facilitates reepithelialization of the cornea in diabetic rat. Diabetes 51: 3055-3062, 2002. 48. Zagon IS, Klocek MS, Sassani JW, and McLaughlin PJ. Use of topical insulin to normalize corneal epithelial healing in diabetes mellitus. Archives of ophthalmology 125: 1082-1088, 2007. 49. Zhang M, Lv XY, Li J, Xu ZG, and Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Experimental diabetes research 2008: 704045, 2008. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46973 | - |
dc.description.abstract | 隨著全球糖尿病人口的快速增加,糖尿病眼角膜病變也越來越受到重視。一般傳統的眼科治療對糖尿病眼角膜病變之療效通常不佳。近年來在人或是獸醫臨床上局部投予血清眼藥,已成功治療嚴重的眼睛表面疾病,如嚴重的乾眼症或是持續性上皮缺損等。本研究之目的在探討不同血清濃度的眼藥(100%、50%、20%) 對於正常與糖尿病大鼠之眼角膜傷口癒合過程的影響。本研究以鏈脲黴素 (streptozotocin) 誘導第一型糖尿病大鼠。之後在手術顯微鏡下分別對12週齡的正常大鼠與22週齡的第一型糖尿病大鼠 (糖尿病後13週) 建立雙眼眼角膜直徑5mm的傷口,建立眼角膜傷口後,分別局部投予三種不同濃度的成體血清 (100%、50%、20%) 眼藥,連續2天 (一天7次)。傷口以1%的螢光染劑進行染色,並在有數位相機裝置的立體顯微鏡下,進行眼角膜上皮傷口觀察與紀錄。
實驗結果顯示在第一型糖尿病大鼠中,50%血清濃度對於眼角膜上皮傷口癒合效果較20%者好;而不同血清濃度對正常大鼠之眼角膜傷口癒合則無顯著差異。此外本實驗以鏈脲黴素藥物在食源性肥胖大鼠中,建立穩定併發有糖尿病眼角膜病變的第二型糖尿病大鼠,發現在患糖尿病後16週前,沒有明顯眼角膜上皮傷口延遲癒合的現象。而在糖尿病後22週的第二型糖尿病大鼠,觀察到眼角膜上皮傷口延遲癒合的現象,並局部投予不同濃度的血清,結果顯示100%血清濃度對於眼角膜上皮傷口癒合有較20%者好之趨勢。在本研究結果顯示,不同血清濃度局部點眼對正常或糖尿病動物模式的眼角膜上皮傷口癒合之影響顯著不同。在正常動物模式中,不同血清濃度對眼角膜上皮傷口癒合速率沒有顯著差異;在糖尿病動物模式中,高濃度 (100%或50%) 的血清對於眼角膜上皮傷口癒合,較低濃度 (20%) 者好,可以提供臨床使用局部血清眼藥治療時,血清濃度選擇的依據。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:44:24Z (GMT). No. of bitstreams: 1 ntu-99-R97643010-1.pdf: 1107403 bytes, checksum: b6f13dd910a3c2c3d433899822eb237d (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 摘要 IV
ABSTRACT V 第一章 序言 1 第二章 文獻探討 4 第一節 眼角膜的結構及生理簡介 4 第二節 維持眼角膜上皮細胞健康的生長因子 6 第三節 眼角膜上皮傷口癒合機制 11 第四節 糖尿病眼角膜病變的介紹 13 第五節 已知可促進眼角膜上皮傷口癒合的局部療法 16 第三章 實驗材料與方法 22 第一節 不同濃度同源血清對正常與第一型糖尿病大鼠眼角膜上皮癒合的影響 22 第二節 伴隨糖尿病眼角膜病變之第二型糖尿病大鼠模式建立及不同濃度血清對其眼角膜上皮癒合的影響 26 第四章 實驗結果 29 第一節 第一型及第二型糖尿病大鼠模式的建立 29 第二節 伴隨糖尿病眼角膜病變之第二型糖尿病大鼠模式的建立 33 第三節 局部點眼不同濃度同源血清對眼角膜上皮癒合的影響 34 第五章 討論 36 第一節 第一型與第二型糖尿病大鼠模式的建立 36 第二節 伴隨有糖尿病眼角膜病變之糖尿病大鼠模式的建立 38 第三節 同源血清臨床的運用與其限制 40 第四節 局部點眼不同濃度同源血清對於眼角膜上皮癒合的影響 43 第五節 本研究的限制或缺點 45 第六章 結論 46 第七章 參考文獻 58 | |
dc.language.iso | zh-TW | |
dc.title | 不同濃度同源血清局部點眼對於正常與糖尿病大鼠眼角膜上皮癒合的影響 | zh_TW |
dc.title | Effects of Topical Homologous Serum with Different Concentrations on Corneal Epithelium Wound Healing in Normal and Diabetic Rats | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳偉勵 | |
dc.contributor.oralexamcommittee | 葉力森,詹東榮,劉振軒 | |
dc.subject.keyword | 糖尿病眼角膜病變,血清眼藥,眼角膜上皮傷口癒合,第一型糖尿病大鼠,第二型糖尿病大鼠, | zh_TW |
dc.subject.keyword | diabetic keratopathy,serum eye drops,corneal epithelium wound healing,type I diabetic rats,type II diabetic rats, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-19 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床動物醫學研究所 | zh_TW |
顯示於系所單位: | 臨床動物醫學研究所 |
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