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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 周晉澄 | |
| dc.contributor.author | Sun-Yu Fu | en |
| dc.contributor.author | 傅孫鈺 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:43:50Z | - |
| dc.date.available | 2010-08-20 | |
| dc.date.copyright | 2010-08-20 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-19 | |
| dc.identifier.citation | Archer, G., 1998. Staphylococcus aureus: A well-armed pathogen. Clin Infect Dis. 26, 1179– 1180
Balaban, N., Rasooly, A., 2000. Staphylococcal enterotoxins. Int. J. Food. Microbiol. 61, 1–10. Bania, J., Dabrowska, A., Bystron, J., Korzekwa, K., Chrzanowska, J., Molenda, J., 2006. Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food. Int. J. Food. Microbiol. 108, 36–41. Becker, K., Friedrich, A.W., Lubritz, G., Weilert, M., Peters, B., von Eiff, C., 2003. Prevalence of genes coding pyrogenic toxin superantigens and exfoliative toxins among strains of isolated from blood and nasal specimens. J. Clin. Microbiol. 41, 1434–1439. Becker, K., Friedrich, A.W., Peters, G., von Eiff, C., 2004. Systematic survey on the prevalence of genes coding for staphylococcal enterotoxins SElM, SElO, and SElN. Mol. Nutr. Food. Res. 48, 488–495. Becker, K., Keller, B., von Eiff, C., Brück, M., Lubritz, G., Etienne, J., Peters, G., 2001. Enterotoxigenic potential of Staphylococcus intermedius. Appl. Environ. Microbiol. 67, 5551–5557. Becker. K., Roth, R., Peters, G., 1998. Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J. Clin. Microbiol. 36, 2548–2553. Bendahou, A., Abid, M., Bouteldoun, N., Catelejine, D., Lebbadi, M., 2009. Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, lben and jben, in northern Morocco. J. Infect. Dev. Ctries. 3, 169–176. Betley, M.J., Mekalanos, J.J., 1988. Nucleotide sequence of the type A staphylococcal enterotoxin gene. J. Bacteriol. 170, 34–41. Bohach, G.A., Kreiswirth, B.N., Novick, R.P., Schlievert, P.M., 1989. Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B and C1 with use of Southern hybridization and immunologic assays. Rev. Infect. Dis. 11, S75–S82. Bonness, S., Szekat, C., Novak, N., Bierbaum, G., 2008. Pulsed-field gel electrophoresis of Staphylococcus aureus isolates from atopic patients revealing presence of similar strains in isolates from children and their parents. J. Clin. Microbiol. 46, 456–461. Boost, M., O'Donoghue, M., James, A., 2008. Investigation of the role of dogs as reservoirs of Staphylococcus aureus and the transmission of strains between pet owners and their dogs. Hong Kong Med. J. 15–18. Brakstad, O.G., Aasbakk, K., Maeland, J.A., 1992. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol. 30, 1654–1660. Campbell, S.J., Deshmukh, H.S., Nelson, C.L., Bae, I.G., Stryjewski, M.E., Federspiel, J.J., Tonthat, G.T., Rude, T.H., Barriere, S.L., Corey, R., Fowler, V.G. Jr., 2008. Genotypic characteristics of Staphylococcus aureus isolates from a multinational trial of complicated skin and skin structure infections. J. Clin. Microbiol. 46, 678–684. Cefai, C., Ashurst, S., Owens, C., 1994. Human carriage of methicillin-resistant Staphylococcus aureus linked with pet dog. Lancet. 344, 539–540. Cha, J.O., Lee, J.K., Jung, Y.H., Yoo, J.I., Park, Y.K., Kim, B.S., Lee, Y.S., 2005. Molecular analysis of Staphylococcus aureus isolates associated with staphylococcal food poisoning in South Korea. J. Appl. Microbiol. 101, 864–871. Chiang, Y.C., Liao, W.W., Fan, C.M., Pai, W.Y., Chiou, C.S., Tsen, H.Y., 2008. PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int. J. Food Microbiol. 121, 66–73. Cooke, F.J., Brown, N.M., 2010. Community-associated methicillin-resistant Staphylococcus aureus infections. Br. Med. Bull. 94, 215–227. Denny, C.B., Humber, J.Y., Bohrer, C.W., 1971. Effect of toxin concentration on the heat inactivation of staphylococcal enterotoxin A in beef bouillon and in phosphate buffer. Appl. Microbiol. 21, 1064–1066. Devore-Carter, D., Kar, S., Vellucci, V., Bhattacherjee, V., Domanski, P., Hostetter, M.K., 2008. Superantigen-like effects of a Candida albicans polypeptide. J. Infect. Dis. 197, 981–989. Dice, L.R., 1945. Measures of the amount of ecologic association between specie. In: Ecology. 26, 297–302. Dinges, M.M., Orwin, P.M., Schlievert, P.M., 2000. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 13, 16-34. Evans, A.G., Moser, J.M., Krug, L.T., Pozharskaya, V., Mora, A.L., Speck, S.H., 2008. A gammaherpes virus-secreted activator of V beta4+ CD8+ T cells regulates chronic infection and immunopathology. J. Exp. Med. 205, 669–684. Evenson, M.L., Hinds, M.W., Bernstein, R.S., Bergdoll, M.S., 1988. Estimation of human dose of staphylococcal enterotoxin A from a large outbreak of staphylococcal food poisoning involving chocolate milk. Int. J. Food. Microbiol. 7, 311–316. Faires, M.C., Tater, K.C., Weese, J.S., 2009. An investigation of methicillin-resistant Staphylococcus aureus colonization in people and pets in the same household with an infected person or infected pet. J. Am. Vet. Med. Assoc. 235, 540–543. Fessler, A., Scott, C., Kadlec, K., Ehricht, R., Monecke, S., Schwarz, S., 2010. Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis. J. Antimicrob. Chemother. 65, 619–625. Genigeorgis, C.A., 1989. Present state of knowledge on staphylococcal intoxication. Int. J. Food. Microbiol. 9, 327–360. Hamdan-Partida, A., Sainz-Espuñes, T., Bustos-Martínez, J., 2010. Characterization and persistence of Staphylococcus aureus strains isolated from the anterior nares and throats of healthy carriers in a Mexican community. J. Clin. Microbiol. 48, 1701–1705. Haveri, M., Roslöf, A., Rantala, L., Pyörälä, S., 2007. Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. J. Appl. Microbiol. 103, 993–1000. Hilker, J.S., Heilman, W.R., Tan, P.L., Denny, C.B., Bohrer, C.W., 1968. Heat inactivation of enterotoxin A from Staphylococcus aureus in veronal buffer. Appl. Microbiol. 16, 308–310. Holtfreter, S., Grumann, D., Schmudde, M., Nguyen, H.T., Eichler, P., Strommenger, B., Kopron, K., Kolata, J., Giedrys-Kalemba, S., Steinmetz, I., Witte, W., Bröker, B.M., 2007. Clonal distribution of superantigen genes in clinical Staphylococcus aureus isolates. J. Clin. Microbiol. 45, 2669–2680. Hsieh, J.M., Chen, R.S., Tsai, T.Y., Pan, T.M., Chou, C.C., 2008. Phylogenetic analysis of livestock oxacillin-resistant Staphylococcus aureus. Vet. Microbiol. 126, 234–242. Hu, D.L., Omoe, K., Shimoda, Y., Nakane, A., Shinagawa, K., 2003. Induction of emetic response to staphylococcal enterotoxins in the house musk shrew (Suncus murinus). Infect. Immun. 71, 567–570. Hu, D.L., Omoe, K., Inoue, F., Kasai, T., Yasujima, M., Shinagawa, K., Nakane, A., 2008. Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates. J. Med. Microbiol. 57, 1106–1112. Huber, B.T., Hsu, P.N., Sutkowski, N., 1996. Virus-encoded superantigens. Microbiol. Rev. 60, 473–482. Jarraud, S., Peyrat, M.A., Lim, A., Tristan, A., Bes, M., Mougel, C., Etienne, J., Vandenesch, F., Bonneville, M., Lina, G., 2001. egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus. J. Immunol. 166, 669–677. Johnson, W.M., Tyler, S.D., Ewan, E.P., Ashton, F.E., Pollard, D.R., Rozee, K.R., 1991. Detection of genes for enterotoxins, exfoliative toxins, and toxin shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J. Clin. Microbiol. 29, 426– 430. Kadlec, K., Ehricht, R., Monecke, S., Steinacker, U., Kaspar, H., Mankertz, J., Schwarz, S., 2009. Diversity of antimicrobial resistance pheno- and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine. J. Antimicrob. Chemother. 64, 1156–1164. Kérouanton, A., Hennekinne, J.A., Letertre, C., Petit, L., Chesneau, O., Brisabois, A., De Buyser, M.L., 2007. Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int. J. Food. Microbiol. 115, 369–375. Lämmler, Ch., Kneden, Ӧ., Annemüller, C., Wolter, W., Zschӧck, M., 2000. Molecular analysis of virulence factors of Staphylococcus aureus from bovine subclinical mastitis. In: Symposium Immunology of Ruminant Mammary Gland, Stresa, 326–330. Larkin, E.A., Carman, R.J., Krakauer, T., Stiles, B.G., 2009. Staphylococcus aureus: the toxic presence of a pathogen extraordinaire. Curr. Med. Chem. 16, 4003–4019. Lee, I.C., Stevenson, K.E., Harmon, L.G., 1977. Effect of beef broth protein on the thermal inactivation of staphylococcal enterotoxin B1. Appl. Environ. Microbiol. 33, 341–344. Letertre, C., Perelle, S., Dilasser, F., Fach, P., 2003. Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. J. Appl. Microbiol. 95, 38–43. Lina, G., Bohach, G.A., Nair, S.P., Hiramatsu, K., Jouvin-Marche, E. Mariuzza, R., 2004. Standard nomenclature for the superantigens expressed by Staphylococcus. J. Infect. Dis. 189, 2334–2336. Manian, F.A., 2003. Asymptomatic nasal carriage of mupirocin-resistant, methicillin-resistant Staphylococcus aureus (MRSA) in a pet dog associated with MRSA infection in household contacts. Clin. Infect. Dis. 36, e26–28. Marrack, P., Kappler, J., 1990. The staphylococcal enterotoxin and their relatives. Science. 248, 705–711. McDougal, L.K., Steward, C.D., Killgore, G.E., Chaitram, J.M., McAllister, S.K., Tenover, F.C., 2003. Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the united states: establishing a national database. J. Clin. Microbiol. 41, 5113–5120. Monday, S.R., Bohach, G.A., 1999. Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. J. Clin. Microbiol. 37, 3411–3414. Morandi, S., Brasca, M., Andrighetto, C., Lombardi, A., Lodi, R., 2009. Phenotypic and genotypic characterization of Staphylococcus aureus strains from italian dairy products. Int. J. Microbiol. 501362. Munson, S.H., Tremaine, M.T., Betley, M.J., Welch, R.A., 1998. Identification and characterization of staphylococcal enterotoxin types G and I from Staphylococcus aureus. Infect. Immun. 66, 3337–3348. Mørk, T., Kvitle, B., Mathisen, T., Jørgensen, H.J., 2010. Bacteriological and molecular investigations of Staphylococcus aureus in dairy goats. Vet. Microbiol. 141, 134–141. Nashev, D., Toshkova, K., Bizeva, L., Akineden, O., Lämmler, C., Zschöck, M., 2007. Distribution of enterotoxin genes among carriage- and infection-associated isolates of Staphylococcus aureus. Lett. Appl. Microbiol. 45, 681–685. Oehler, R.L., Velez, A.P., Mizrachi, M., Lamarche, J., Gompf, S., 2009. Bite-related and septic syndromes caused by cats and dogs. Lancet. Infect. Dis. 9, 439–447. Omoe, K., Hu, D.L., Takahashi-Omoe, H., Nakane, A., Shinagawa, K., 2003. Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids. Infect. Immun. 71, 6088–6094. Omoe, K., Hu, D.L., Takahashi-Omoe, H., Nakane, A., Shinagawa, K., 2005. Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. FEMS. Microbiol. Lett. 246, 191–198. Omoe, K., Imanishi, K., Hu, D.L., Kato, H., Fugane, Y., Abe, Y., Hamaoka, S., Watanabe, Y., Nakane, A., Uchiyama, T., Shinagawa, K., 2005. Characterization of novel staphylococcal enterotoxin-like toxin type P. Infect. Immun. 73, 5540–5546. Omoe, K., Imanishi, K., Hu, D.L., Kato, H., Takahashi-Omoe, H., Nakane, A., Uchiyama, T., Shinagawa, K., 2004. Biological properties of staphylococcal enterotoxin-like toxin type R. Infect. Immun. 72, 3664–3667. Omoe, K., Ishikawa, M., Shimoda, Y., Hu, D.L., Ueda, S., Shinagawa, K., 2002. Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harbouring seg, seh, or sei genes. J. Clin. Microbiol. 40, 857–862. Ono, H.K., Omoe, K., Imanishi, K., Iwakabe, Y., Hu, D.L., Kato, H., Saito, N., Nakane, A., 2008. Uchiyama T, Shinagawa K. Identification and characterization of two novel staphylococcal enterotoxins, types S and T. Infect. Immun. 76, 4999–5005. Orwin, P.M., Fitzgerald, J.R., Leung, D.Y., Gutierrez, J.A., Bohach, G.A., Schlievert, P.M., 2003. Characterization of Staphylococcus aureus enterotoxin L. Infect. Immun. 71, 2916–2919. Orwin, P.M., Leung, D.Y., Donahue, H.L., Novick, R.P., Schlievert, P.M., 2000. Biochemical and biological properties of Staphylococcal enterotoxin K. Infect. Immun. 69, 360–366. Orwin, P.M., Leung, D.Y., Tripp, T.J., Bohach, G.A., Earhart, C.A., Ohlendorf, D.H., Schlievert, P.M., 2002. Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins. Biochemistry. 41, 14033–14040. Ostyn, A., De Buyser, M.L., Guillier, F., Groult, J., Felix, B., Salah, S., Delmas, G., Hennekinne, J.A., 2010. First evidence of a food poisoning outbreak due to staphylococcal enterotoxin type E, France, 2009. Euro. Surveill. 15, 10–13. Pan, Y.Q., Ding, D., Li, D.X., Chen, S.Q., 2007. Expression and bioactivity analysis of Staphylococcal enterotoxin M and N. Protein. Expr. Purif. 56, 286–292. Park, C.E., Szabo, R., 1986. Evaluation of the reversed passive latex agglutination (RPLA) test kits for detection of staphylococcal enterotoxins A, B, C, and D in foods. Can J Microbiol. 32, 723–727. Peles, F., Wagner, M., Varga, L., Hein, I., Rieck, P., Gutser, K., Keresztúri, P., Kardos, G., Turcsányi, I., Béri, B., Szabó, A., 2007. Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary. Int. J. Food. Microbiol. 118, 186–193. Proft, T., Fraser, J.D., 2003. Bacterial superantigens. Clin. Exp. Immunol. 133, 299–306. Růzicková, V., Karpísková, R., Pantůcek, R., Pospísilová, M., Cerníková, P., Doskar, J., 2008. Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic. Int. J. Food Microbiol. 121, 60–65. Sakoulas, G., Gold, H.S., Venkataraman, L., DeGirolami, P,C., Eliopoulos, G.M., Qian, Q., 2001. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains. J. Clin. Microbiol. 39, 3946–3951. Schlievert, P.M., Jablonski, L.M., Roggiani, M., Sadler, I., Callantine, S., Mitchell, D.T., Ohlendorf, D.H., Bohach, G.A., 2000. Pyrogenic toxin superantigen site specificity in toxic shock syndrome and food poisoning in animals. Infect. Immun. 68, 3630–3634. Shio, M.T., Olivier, M., Jancar, S., Ribeiro-Dias, F., 2008. Crucial cytokine interactions in nitric oxide production induced by Mycoplasma arthritidis superantigen. Microbes. Infect. 10, 1543–1551. Smith, J.L., Buchanan, R.L., Palumbo, S.A., 1983. Effect of food enviroment on staplylococcal enterotoxin synthesis: a review. J. Food. Prot. 46, 545–555. Smyth, D.S., Hartigan, P.J., Meaney, W.J., Fitzgerald, J.R., Deobald, C.F., Bohach, G.A., Smyth, C.J., 2005. Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry. J. Med. Microbiol. 54, 401–411. Su, Y.C., Wong, A.C., 1995. Identification and purification of a new staphylococcal enterotoxin, H. Appl. Environ. Microbiol. 61, 1438–1443. Subedi, A., Ubeda, C., Adhikari, R.P., Penadés, J.R., Novick, R.P., 2007. Sequence analysis reveals genetic exchanges and intraspecific spread of SaPI2, a pathogenicity island involved in menstrual toxic shock. Microbiology. 153, 3235–3245. Sutkowski, N., Palkama, T., Ciurli, C., Sekaly, R.P., Thorley-Lawson, D.A., Huber, B.T., 1996. An Epstein-Barr virus-associated superantigen. J. Exp. Med. 184, 971–980. Tamaparu, S., McKillip, J., Drake, M., 2001. Development of a polymerase chain reaction assay for detection and differentiation of Staphylococcus aureus in dairy products. J. Food. Prot. 64, 664–668. Tavares, D.A., Sá-Leão, R., Miragaia, M., de Lencastre, H., 2010. Large screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal. BMC. Infect. Dis. 10, 110–117. Tenover, F.C., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H., Swaminathan, B., 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33, 2233–2239. Thomas, D.Y., Jarraud, S., Lemercier, B., Cozon, G., Echasserieau, K., Etienne, J., Gougeon, M.L., Lina, G., Vandenesch, F., 2006. Staphylococcal enterotoxin-like toxins U2 and V, two new staphylococcal superantigens arising from recombination within the enterotoxin gene cluster. Infect. Immun. 74, 4724–4734. van Belkum, A., Melles, D.C., Snijders, S.V., van Leeuwen, W.B., Wertheim, H.F., Nouwen, J.L., Verbrugh, H.A., Etienne, J., 2006. Clonal distribution and differential occurrence of the enterotoxin gene cluster, egc, in carriage- versus bacteremia-associated isolates of Staphylococcus aureus. J. Clin. Microbiol. 44, 1555–1557. van Duijkeren, E., Wolfhagen, M.J., Box, A.T., Heck, M.E., Wannet, W.J., Fluit, A.C., 2004. Human-to-dog transmission of methicillin-resistant Staphylococcus aureus. Emerg. Infect. Dis. 10, 2235–2237. Varshney, A.K., Mediavilla, J.R., Robiou, N., Guh, A., Wang, X., Gialanella, P., Levi, M.H., Kreiswirth, B.N., Fries, B.C., 2009. Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York. Appl. Environ. Microbiol. 75, 6839–6849. Vitale, C.B., Gross, T.L., Weese, J.S., 2006. Methicillin-resistant Staphylococcus aureus in cat and owner. Emerg. Infect. Dis. 12, 1998–2000. Wang, S.J., Chow, L.W., Wu, M.J., 2002. Multiplex PCR for the simultaneous detection of the SEA, SEB, SED, and SEE genes of enterotoxigenic Staphylococcus aureus. J. Food and Drug Analysis. 10, 164–169. Weese, J.S., Dick, H., Willy, B.M., McGeer, A., Kreiswirth, B.N., Innis, B., 2006. Suspected transmission of methicillin-resistant Staphylococcus aureus between domestic pets and humans in veterinary clinics and in the household. Vet. Microbiol. 115, 148–155. Wieneke, A.A., Roberts, D., Gilbert, R.J., 1993. Staphylococcal food poisoning in the United Kingdom, 1969–1990. Epidemiol. Infect. 110, 519–531. Zhang, S., Iandolo, J.J., Stewart, G.C., 1998. The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant (sej). FEMS. Microbiol. Lett. 168, 227–233. Zhao, J., Hayashi, T., Saarinen, S., Papageorgiou, A.C., Kato, H., Imanioshi, K., Kirikae, T., Abe, R., Uchiyama, T., Miyoshi-Akiyama, T., 2007. Cloning, expression, and characterization of the superantigen streptococcal pyrogenic exotoxin G from Streptococcus dysgalactiae. Infect. Immun. 75, 1721–1729. Zouharova, M., Rysanek, D., 2008. Multiplex PCR and RPLA Identification of Staphylococcus aureus enterotoxigenic strains from bulk tank milk. Zoonoses. Public. Health. 55, 313–319. Zschöck, M., Kloppert, B., Wolter, W., Hamann, H.P., Lämmler, Ch., 2005. Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis. Vet. Microbiol. 108, 243–249. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46947 | - |
| dc.description.abstract | 本研究針對台灣北部地區（台北市、台北縣、桃園縣），無金黃色葡萄球菌感染臨床症狀之寵物及其畜主之鼻腔以棉棒採樣，分離出金黃色葡萄球菌並檢測其腸毒素之基因型，並使用逆向被動乳膠凝集法（reverse passive latex agglutination, RPLA）檢測其傳統型腸毒素A、B、C、D、E之表現型，及使用限制酵素SmaI切割細菌之DNA後，進行脈衝式膠體電泳（Pulse-field gel electrophoresis, PFGE）分析菌株之相關性，並且作抗生素感受性試驗，以了解不同腸毒素基因型和抗藥性與其脈衝式膠體電泳分析分析結果之關係。結果共分離到116株金黃色葡萄球菌，之中有74株（63.8%）至少帶有一種腸毒素基因，最常被偵測到的腸毒素基因型為sea、seb、sek、sep、seq、egc等，另外，採集於人的分離株（95株）帶有腸毒素基因之比例（69.5%），顯著高於分離自寵物（狗與貓）之分離株（21株）（38.1%）（P < 0.05）。本研究分離得到的116株金黃色萄球菌中，鑑定出23株為抗methicillin金黃色葡萄球菌（Methicillin-resistant Staphylococcus aureus, MRSA），另外93株為對methicillin具感受性之金黃色葡萄球菌（Methicillin-sensitive Staphylococcus aureus, MSSA），並且MRSA有顯著高的比率帶有腸毒素基因（91.3%），MSSA的比率則較低（57%）（P < 0.01）。本研究有45.7%的分離株帶有與葡萄球菌性食物中毒有重要相關性的傳統型腸毒素基因型，這些菌株為引起葡萄球菌性食物中毒之可能潛在的危險因子。在脈衝式膠體電泳的相關性樹狀圖分析中，許多腸毒素基因如seb、sed、sek、sep、seq、egc可以發現帶有這些基因的菌株之間彼此可見有群聚現象，推測腸毒素的基因主要藉由菌株傳播（clonal spread）來散佈，因此特定的病原性菌株會有他們的地域性及族群分布特性，而脈衝式膠體電泳可能為病原株的追蹤有所助益。 | zh_TW |
| dc.description.abstract | In order to investigate the Staphylococcus aureus enterotoxin genotypes and phylogenetic relatedness, samples were collected with nasal swab from pet and their owners and using Pulse-field gel electrophoresis (PFGE) patterns of these isolates to analyze their phylogenetic relatedness with enterotoxin genotypes and antimicrobial susceptibility. The commercial RPLA test kit SET-RPLA detecting enterotoxins A, B, C, D, and E was available for this study to compare with the strain genotypes. The results of our previous sampling show that the strains of methicillin-resistant Staphylococcus aureus (MRSA) have a higher percentage (91.3%) of harboring enterotoxin genes than the methicillin-sensitive S. aureus (MSSA) do (57%) (P < 0.01), and the most frequent enterotoxin genotypes detected from free clinical complains pets and humans are sea, seb, sek, sep, seq and egc. To compare the se gene prevalence of isolates from human and pet, the human origins (66/95, 69.5%) were higher than the pet origins (8/21, 38.1%) (P < 0.05). Certain degree of relatedness between enterotoxin genotypes and PFGE phylogenetic dendrogram has been observed, and several enterotoxin genotypes, such as seb, sed, sek, sep, seq and egc have a trend to clump themselves into 60 - 100% cluster similarity. Perhaps that can explain why the distributions of se genes are variable with different origins and areas. Antimicrobial susceptivity test show that MRSAs had a higher resistance to the antibiotics than the MSSAs, and some resistance strains have high phylogenetic relatedness. As these results, S. aureus enterotoxin genes and antimicrobial resistance presents clustered appearance, for the reason that the pathogenic strains should also have their regional and clonal distribution and that would be helpful for tracing the source of pathogenic strain. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:43:50Z (GMT). No. of bitstreams: 1 ntu-99-R97629019-1.pdf: 1124916 bytes, checksum: ec1632d5dbe0dafcf0a12a6670c13e0b (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 誌謝.................................................................................................................................i
中文摘要........................................................................................................................ii 英文摘要.......................................................................................................................iii 目錄................................................................................................................................v 表目錄..........................................................................................................................vii 圖目錄.........................................................................................................................viii 第一章 緒言................................................................................................................1 第二章 文獻探討 2.1 超級抗原（superantigen）之作用機制..............................................................3 2.2 各種新型腸毒素之基本生化特性.....................................................................3 2.3 腸毒素之檢測方法.............................................................................................9 2.4 脈衝式膠體電泳對於金黃色萄球菌之應用...................................................12 2.5 金黃色葡萄球菌腸毒素之流行病學概...........................................................14 2.6 研究動機...........................................................................................................17 第三章 材料與方法 3.1 採樣方法以及細菌分離與培養.......................................................................19 3.2 以PCR方法偵測20種金黃色葡萄球菌之腸毒素基因型.............................20 3.2.1 腸毒素基因型sea、seb、sec、sed、see偵測.................................................21 3.2.2 腸毒素基因型seg、seh、sei、sej偵測.........................................................22 3.2.3 腸毒素基因型sel、 sem、sen、seo、seq偵測..............................................22 3.2.4 腸毒素基因型sek偵測..............................................................................23 3.2.5 腸毒素基因型sep偵測.............................................................................23 3.2.6 腸毒素基因型ser偵測..............................................................................23 3.2.7 腸毒素基因型ses偵測..............................................................................24 3.2.8 腸毒素基因型set偵測..............................................................................24 3.2.9 腸毒素基因型seu偵測..............................................................................24 3.3逆向被動乳膠凝集法（RPLA）偵測腸毒素......................................................25 3.4 脈衝式膠體電泳（PFGE）之樣品置備.............................................................25 3.5 SmaI 限制酵素切割..........................................................................................26 3.6 脈衝式膠體電泳之電泳條件...........................................................................26 3.7 脈衝式膠體電泳下菌株之間的關聯分析.......................................................26 3.8 抗生素感受性試驗...........................................................................................27 3.9 統計分析...........................................................................................................27 第四章 結果 4.1 分離株之數量及來源.......................................................................................30 4.2 Multiplex-PCR鑑定二十種腸毒素基因型.......................................................30 4.3逆向被動乳膠凝集法（RPLA）偵測腸毒素A、B、C、D、E表現型....................33 4.4 抗生素感受性試驗...........................................................................................42 4.5 脈衝式膠體電泳分析.......................................................................................49 第五章 討論..............................................................................................................52 第六章 未來展望......................................................................................................59 第七章 參考文獻......................................................................................................60 附錄一 採樣同意書..................................................................................................71 附錄二 採樣基本資料..............................................................................................72 附錄三 採樣步驟......................................................................................................73 附錄四 116株由台灣北部地區之寵物及其畜主分離之金黃色葡萄球菌脈衝式膠體電泳相似度百分比.................................................................................74 | |
| dc.language.iso | zh-TW | |
| dc.subject | 脈衝式膠體電泳 | zh_TW |
| dc.subject | 金黃色葡萄球菌 | zh_TW |
| dc.subject | 腸毒素基因 | zh_TW |
| dc.subject | 抗生素感受性試驗 | zh_TW |
| dc.subject | 相關性樹狀圖 | zh_TW |
| dc.subject | Staphylococcus aureus | en |
| dc.subject | Antimicrobial susceptivity test | en |
| dc.subject | Phylogenetic dendrogram pattern | en |
| dc.subject | Pulse-field gel electrophoresis | en |
| dc.subject | Enterotoxin genes | en |
| dc.title | 北台灣寵物及其畜主之金黃色葡萄球菌腸毒素之分子流行病學研究 | zh_TW |
| dc.title | Molecular Epidemiology of Enterotoxins of Staphylococcus aureus from Pets and Their Owners in Northern Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張照勤,潘子明,周崇熙,張紹光 | |
| dc.subject.keyword | 金黃色葡萄球菌,腸毒素基因,脈衝式膠體電泳,相關性樹狀圖,抗生素感受性試驗, | zh_TW |
| dc.subject.keyword | Staphylococcus aureus,Enterotoxin genes,Pulse-field gel electrophoresis,Phylogenetic dendrogram pattern,Antimicrobial susceptivity test, | en |
| dc.relation.page | 75 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-20 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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