請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46489完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 童心欣(Hsin-Hsin Tung) | |
| dc.contributor.author | Yu-Han Chien | en |
| dc.contributor.author | 簡宇涵 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:11:44Z | - |
| dc.date.available | 2012-08-09 | |
| dc.date.copyright | 2010-08-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-23 | |
| dc.identifier.citation | Anthony, C. (1982). 'The Biochemistry of Methylotrophs, Academic press, London.'
ATSDR (1989). 'Toxicological profile for n-Nitrosodimethylamine. Agency for Toxic Substances and Disease Registry (ATSDR), U.S. Public Health Service In collaboration with U.S. Environmental Protection Agency , December.'. Bracklow, U., Drew, A., Vocks, M., Kraume, M. (2007). Comparison of nutrients degradation in small scale membrane bioreactors fed with synthetic/domestic wastewater, Elsevier Science Bv. Bradley, P. M., Carr, Steve A., Baird, Rodger B., Chapelle, Francis H. (2005). 'Biodegradation of N-nitrosodimethylamine in soil from a water reclamation facility.' Bioremediation Journal 9(2): 115-120. Bruins, M. R., Kapil, S., Oehme, F. W. (2000). 'Pseudomonas pickettii: A common soil and aerobic groundwater bacteria with pathogenic and biodegradation properties.' Ecotoxicology and Environmental Safety 47(2): 105-111. CDPH (2002). 'California Department of Public Health ; Drinking Water Notification Levels and Response Levels: An Overview. http://www.cdph.ca.gov/certlic/drinkingwater/Documents/Notificationlevels/NotificationLevels.pdf.' CDPH (2006). 'A Brief History of NDMA Findings in Drinking Water; http://www.cdph.ca.gov/certlic/drinkingwater/Pages/NDMAhistory.aspx.' Choi, J. H., Valentine, R. L. (2002). 'Formation of N-nitrosodimethylamine (NDMA) from reaction of monochloramine: a new disinfection by-product.' Water Research 36(4): 817-824. Dean, J. A. (1985). 'Lange's Handbook of Chemistry', 13th ed. (p10-5). McGraw-Hill Co., New York. Dedysh, S. N., Liesack, W., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Semrau, J. D., Bares, A. M., Panikov, N. S., Tiedje, J. M. (2000). 'Methylocella palustris gen. nov., sp nov., a new methane-oxidizing acidophilic bacterium from peat bags, representing a novel subtype of serine-pathway methanotrophs.' International Journal of Systematic and Evolutionary Microbiology 50: 955-969. Felske, A., Rheims, H., Wolterink, A., Stackebrandt, E., Akkermans, A. D. L. (1997). 'Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils.' Microbiology-Uk 143: 2983-2989. Fiddler, W., Pensabene, J. W., Doerr, R. C., Dooley, C. J. (1977). 'PRESENCE OF DIMETHYL-NITROSAMINES AND DIETHYL-NITROSAMINES IN DEIONIZED WATER.' Food and Cosmetics Toxicology 15(5): 441-443. Fournier, D., Hawari, J., Halasz, A., Streger, S. H., McClay, K. R., Masuda, H., Hatzinger, P. B. (2009). 'Aerobic Biodegradation of N-Nitrosodimethylamine by the Propanotroph Rhodococcus ruber ENV425.' Applied and Environmental Microbiology 75(15): 5088-5093. Fournier, D., Hawari, J., Streger, S. H., McClay, K., Hatzinger, P. B. (2006). 'Biotransformation of N-nitrosodimethylamine by Pseudomonas mendocina KR1.' Applied and Environmental Microbiology 72(10): 6693-6698. Gregory A. Brusseau, Tsien, H. C., Richard S. Hanson & Lawrence P. Wackett* (1990). 'Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity.' Biodegradation 1. Hamptom, D., Zatman, L.J (1973). 'The metabolism to tetramethylammonium chloride by bacterium 5H2.' Biochemical Society Transactions 1: 667-668. Han, J. I., Lontoh, S., Semrau, J. D. (1999). 'Degradation of chlorinated and brominated hydrocarbons by Methylomicrobium album BG8.' Archives of Microbiology 172(6): 393-400. Hanson, R. S., Hanson, T. E. (1996). 'Methanotrophic bacteria.' Microbiological Reviews 60(2): 439-+. Horvath, R. S. (1972). 'MICROBIAL CO-METABOLISM AND DEGRADATION OF ORGANIC COMPOUNDS IN NATURE.' Bacteriological Reviews 36(2): 146-&. IARC (1978). 'International Agency for Research on Cancer monographs on the evaluation of the carcinogenic risk of chemicals to humans. Some N-nitroso compounds ' 17: 125-175. Kaplan, D. L., Kaplan, A. M. (1985). 'BIODEGRADATION OF N-NITROSODIMETHYLAMINE IN AQUEOUS AND SOIL SYSTEMS.' Applied and Environmental Microbiology 50(4): 1077-1086. Kemper, J. M., Westerhoff, P., Dotson, A., Mitch, W. A. (2009). 'Nitrosamine, Dimethylnitramine, and Chloropicrin Formation during Strong Base Anion-Exchange Treatment.' Environmental Science & Technology 43(2): 466-472. Kimoto, W. I., Dooley, C. J., Carre, J., Fiddler, W. (1980). 'ROLE OF STRONG ION-EXCHANGE RESINS IN NITROSAMINE FORMATION IN WATER.' Water Research 14(7): 869-876. Koh, S. C., Bowman, J. P., Sayler, G. S. (1993). 'SOLUBLE METHANE MONOOXYGENASE PRODUCTION AND TRICHLOROETHYLENE DEGRADATION BY A TYPE-I METHANOTROPH, METHYLOMONAS-METHANICA-68-1.' Applied and Environmental Microbiology 59(4): 960-967. Krauss, M., Longree, P., Dorusch, F., Ort, C., Hollender, J. (2009). 'Occurrence and removal of N-nitrosamines in wastewater treatment plants.' Water Research 43(17): 4381-4391. Lee, S. W., Keeney, D. R., Lim, D. H., Dispirito, A. A., Semrau, J. D. (2006). 'Mixed pollutant degradation by Methylosinus trichosporium OB3b expressing either soluble or particulate methane monooxygenase: Can the tortoise beat the hare?' Applied and Environmental Microbiology 72(12): 7503-7509. Lee, W., Westerhoff, P., Croue, J. P. (2007). 'Dissolved organic nitrogen as a precursor for chloroform, dichloroacetonitrile, N-Nitrosodimethylamine, and trichloronitromethane.' Environmental Science & Technology 41(15): 5485-5490. Liang, S., Min, J. H., Davis, M. K., Green, J. F., Remer, D. S. (2003). 'Use of pulsed-UV processes to destroy NDMA.' Journal American Water Works Association 95(9): 121-131. Loperena, L., Ferrari, M. D., Diaz, A. L., Ingold, G., Perez, L. V., Carvallo, F., Travers, D., Menes, R. J., Lareo, C. (2009). 'Isolation and selection of native microorganisms for the aerobic treatment of simulated dairy wastewaters.' Bioresource Technology 100(5): 1762-1766. Mirvish, S. S. (1975). 'Formation of N-nitroso compounds: Chemistry, kinetics, and in vivo occurrence.' Toxicology and Applied Pharmacology 31(3): 325-351. Mitch, W. A., Sedlak, D. L. (2002). 'Formation of N-nitrosodimethylamine (NDMA) from dimethylamine during chlorination.' Environmental Science & Technology 36(4): 588-595. Mitch, W. A., Sharp, J. O., Trussell, R. R., Valentine, R. L., Alvarez-Cohen, L., Sedlak, D. L. (2003). 'N-nitrosodimethylamine (NDMA) as a drinking water contaminant: A review.' Environmental Engineering Science 20(5): 389-404. Murrell, J. C., Gilbert, B., McDonald, I. R. (2000). 'Molecular biology and regulation of methane monooxygenase.' Archives of Microbiology 173(5-6): 325-332. Najm, I., Trussell, R. R. (2000). 'NDMA formation in water and wastewater. 2000 Water Quality Technology Conference, Salt Lake City, USA.' Pehlivanoglu-Mantas, E., Sedlak, D. L. (2006). 'The fate of wastewater-derived NDMA precursors in the aquatic environment.' Water Research 40(6): 1287-1293. Plumlee, M. H., Lopez-Mesas, M., Heidlberger, A., Ishida, K. P., Reinhard, M. (2008). 'N-nitrosodimethylamine (NDMA) removal by reverse osmosis and UV treatment and analysis via LC-MS/MS.' Water Research 42(1-2): 347-355. Radajewski, S., Ineson, P., Parekh, N. R., Murrell, J. C. (2000). 'Stable-isotope probing as a tool in microbial ecology.' Nature 403(6770): 646-649. Saravia, V., Murro, D., Ferrari, M. D., Lareo, C., Loperena, L. (2004). 'Butter oil as model substrate to evaluate milk fat degrading microorganisms used in bioaugmentation strategies.' Fresenius Environmental Bulletin 13(4): 353-355. Sedlak, D. L., Deeb, R. A., Hawley, E. L., Mitch, W. A., Durbin, T. D., Mowbray, S., Carr, S. (2005). 'Sources and fate of nitrosodimethylamine and its precursors in municipal wastewater treatment plants.' Water Environment Research 77(1): 32-39. Shapley, D. (1976). 'Nitrosamines: Scientists on the Trail of Prime Suspect in Urban Cancer ' Science 191: 268-270 Sharp, J. O., Sales, C. M., LeBlanc, J. C., Liu, J., Wood, T. K., Eltis, L. D., Mohn, W. W., Alvarez-Cohen, L. (2007). 'An inducible propane monooxygenase is responsible for N-nitrosodimethylamine degradation by Rhodococcus sp strain RHA1.' Applied and Environmental Microbiology 73(21): 6930-6938. Sharp, J. O., Wood, T. K., Alvarez-Cohen, L. (2005). 'Aerobic biodegradation of n-nitrosodimethylamine (NDMA) by axenic bacterial strains.' Biotechnology and Bioengineering 89(5): 608-618. Stanley, S. H., Prior, S. D., Leak, D. J., Dalton, H. (1983). 'Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: Studies in batch and continuous cultures ' Biotechnology Letters 5: 487-492. Stefan, M. I., Bolton, J. R. (2002). 'UV direct photolysis of N-nitrosodimethylamine (NDMA): Kinetic and product study.' Helvetica Chimica Acta 85(5): 1416-1426. Tsien, H. C., Brusseau, G. A., Hanson, R. S., Wackett, L. P. (1989). 'BIODEGRADATION OF TRICHLOROETHYLENE BY METHYLOSINUS-TRICHOSPORIUM OB3B.' Applied and Environmental Microbiology 55(12): 3155-3161. Urakami, T., Arakia, H., Kobayashia, H. (1990). 'Isolation and identification of tetramethylammonium-biodegrading bacteria.' Journal of Fermentation and Bioengineering 70(1): 41-44. USEPA (2009). 'Drinking Water Contaminant Candidate List ; Contaminant Candidate List 3 (CCL 3). http://www.epa.gov/safewater/ccl/ccl3.html.' USEPA (2002). 'Integrated Risk Information System. Office of Research and Development (ORD), National Center for Environmental Assessment ; http://www.epa.gov/ncea/iris/subst/0045.htm.' Wilczak, A., Assadi-Rad, A., Lai, H. H., Hoover, L. L., Smith, J. F., Berger, R., Rodigari, F., Beland, J. W., Lazzelle, L. J., Kinicannon, E. G., Baker, H., Heaney, C. T. (2003). 'Formation of NDMA in chloraminated water coagulated with DADMAC cationic polymer.' Journal American Water Works Association 95(9): 94-106. Xu, B. B., Chen, Z. L., et al. (2009a). 'Inhibiting the regeneration of N-nitrosodimethylamine in drinking water by UV photolysis combined with ozonation.' Journal of Hazardous Materials 168(1): 108-114. Xu, B. B., Chen, Z. G., Qi, F., Xu, Z. Z. (2009b). 'Degradation of N-nitrosodimethylamine (NDMA) in water by UV/O-3.' Journal of Water Supply Research and Technology-Aqua 58(2): 135-145. Yang, W. C., Gan, J., Liu, W. P., Green, R. (2005). 'Degradation of N-nitrosodimethylamine (NDMA) in landscape soils.' Journal of Environmental Quality 34(1): 336-341. Yoshinari, T., Shafer, D. (1990). 'DEGRADATION OF DIMETHYL NITROSAMINE BY METHYLOSINUS-TRICHOSPORIUM OB3B.' Canadian Journal of Microbiology 36(12): 834-838. 黃筱涵 (2009). '以生物處理法降解含TMAH廢水之研究.' 國立台灣大學環境工程學研究所碩士論文 環檢所 (2000). '中華民國 環境保護署 環境檢驗所：水中生化需氧量檢測方法 NIEA W510.54B, http://www.niea.gov.tw/niea/WATER/W51054B.htm '. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46489 | - |
| dc.description.abstract | 水處理廠為維持出水品質會在淨水流程中進行消毒以去除致病菌活性，而經由成本以及便利性考量，含氯消毒劑皆優於其他消毒程序，因此多數淨水廠與廢水廠多以含氯消毒劑進行消毒。然而含氯消毒劑的使用可能與天然有機物(Nature Organic Matters, NOMs)反應生成多樣的消毒副產物(Disinfection by-products, DBPs)，其中亞硝基二甲基胺(N-Nitrosodimethylamine, NDMA)即為一新興之消毒副產物。NDMA易在以含氯消毒劑進行消毒之水處理廠中被發現，特別是在含氮量較高的情形下，並且經由文獻指出，NDMA具有造成人體致癌的風險而使得NDMA的去除逐漸被受重視。目前已知NDMA可經由物化程序進行有效之去除，然而考量其去除成本較高，現轉而尋求較具經濟效益之微生物處理方法。目前雖有文獻指出NDMA具有微生物可降解性，但對於可降解NDMA的菌株卻所知甚少，因此本研究期從甲烷氧化菌以及水處理廠中找出可降解NDMA之菌株。
為尋找可有效降解NDMA之菌株，本研究將三株甲烷氧化菌Methylosinus trichosporium OB3b、Methylocystis parvus OBBP和 05A-I-S2以及內湖污水廠之活性污泥和經由四甲基氫氧化銨(Tetramethylammonium Hydroxide, TMAH)馴養過後之活性污泥，進行NDMA降解實驗。 研究結果顯示，甲烷氧化菌在表現單氧化酵素時，可依共代謝作用降解NDMA，而當甲烷氧化菌M. trichosporium OB3b 和 05A-I-S2在分泌sMMO之情形下降解NDMA的效率大於M. parvus OBBP分泌pMMO時。此外兩種試驗之污泥皆被發現具有降解NDMA之能力，然而唯有以TMAH馴養過後之污泥中所分離出之一菌株，Uncultured alpha proteobacterium UP2，具有降解NDMA並同時以其作為碳源之能力。 | zh_TW |
| dc.description.abstract | Chlorination is the most common disinfection method to reduce the activities of pathogens in water treatment process due to its low-cost and effectiveness. However, with the existence of nature organic matters (NOMs), the application of chlorine-contained disinfectants may result in the formation of disinfection byproducts (DBPs) which are of particular concern considering their potential impact on people health.
One of the emerging DBPs, N-Nitrosodimethylamine (NDMA), is considered as a potent carcinogen. It is often found in finished water with chlorine or chloramine applied, especially in water with high nitrogen contents. Thus the removal of NDMA in water treatment process is crucial to ensure the safety of aquatic environment. The removal of NDMA by physical-chemical process has been proved to be effective; however, with consideration of cost, physical-chemical technology may not be the best available strategy, promoting biodegradation as an alternative. Previous literature indicated that NDMA is biodegradable, but with limited information regarding functional bacteria provided. The objective of this study is to identify the bacteria with capability to degrade NDMA. Three methanotrophs, i.e., Methylosinus trichosporium OB3b, Methylocystis parvus OBBP and isolated strain 05A-I-S2, as well as two activated sludges including one from Neihu municipal wastewater treatment plant and the other cultivated by tetramethylammonium hydroxide (TMAH) were chosen to examine their biodegradability of NDMA. The result showed that NDMA was degradable by three methanotrophs through cometabolism along with expression of methane monooxygenase (MMO). Note that the efficiency of degradation was higher to methanotrophs with inducement of the soluble MMO (sMMO) than those with particulate MMO (pMMO) produced. In addition, NDMA can also be biodegraded by both sources of sludge adopted in this study with only one pure strain isolated from the TMAH-cultivated sludge, Uncultured alpha proteobacterium UP2, being capable to utilize NDMA as the carbon source while biodegrading. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:11:44Z (GMT). No. of bitstreams: 1 ntu-99-R96541112-1.pdf: 1394752 bytes, checksum: 2d8d034f314646cc9dc1e53dfd5a9949 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 誌謝 I
摘要 II Abstract IV 圖目錄 IX 表目錄 X 第一章 緒論 1 1.1研究前言 1 1.2研究架構 2 第二章 文獻回顧 4 2.1 亞硝基二甲胺(N-Nitrosodimethylamine, NDMA) 4 2.2 NDMA的來源 5 2.2.1 亞硝化(Nitrosation) : 藉由亞硝酸鹽產生NDMA 5 2.2.2偏二甲肼(Unsymmetrical dimethylhydrazine, UDMH)的氧化 6 2.2.3其他形成途徑 9 2.3 四甲基氫氧化銨(tetramethylammonium hydroxide, TMAH) 10 2.4 甲基氧化菌(Methylotrophs)與甲烷氧化菌(Methanotrophs) 11 2.5 NDMA之處理方法 14 2.6以微生物降解NDMA 15 2.7以共代謝作用降解NDMA時生物轉換及可能的代謝產物 19 第三章實驗材料與方法 23 3.1 細菌及污泥 23 3.2.1甲烷氧化菌之培養 25 3.2.2活性污泥降解測試以及馴養與純化 27 3.2.2.1內湖污水處理廠活性污泥降解測試 28 3.2.2.2以TMAH馴養過之活性污泥降解測試 29 3.2.3活性污泥之馴養與純化 31 3.3微生物濃度與總蛋白質之關係 32 3.4甲烷氧化菌之酵素鑑定 33 3.5 NDMA之降解實驗 34 3.5.1甲烷氧化菌 34 3.5.2 內湖污水廠以及以TMAH馴養後之污泥 34 3.6 高濃度之NDMA分析方法 35 3.7 菌株的鑑定 36 第四章 結果與討論 37 4.1甲烷氧化菌 37 4.1.1甲烷氧化菌之酵素測試 37 4.1.3降解速率 41 4.2內湖污水廠生活污泥與以TMAH馴養過後之污泥 46 4.2.1降解測試 46 4.2.2細菌純化 48 4.2.3分離菌株降解NDMA試驗 48 4.2.4菌種鑑定 49 4.2.5小結 49 第五章 結論與建議 52 5.1結論 52 5.2建議 53 第六章 參考文獻 54 | |
| dc.language.iso | zh-TW | |
| dc.title | N-亞硝基二甲胺之生物降解 | zh_TW |
| dc.title | Biodegradation of N-Nitrosodimethylamine | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林郁真(Angela Yu-Chen Lin),黃良銘 | |
| dc.subject.keyword | 亞硝基二甲基胺,生物降解,甲烷氧化菌,甲烷單氧化酵素, | zh_TW |
| dc.subject.keyword | N-Nitrosodimethylamine (NDMA),Biodegradation,Methanotrophs,Methane Monooxygenase (MMO), | en |
| dc.relation.page | 62 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-23 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| 顯示於系所單位： | 環境工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 目前未授權公開取用 | 1.36 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。