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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳文中(Wen-Jong Wu) | |
| dc.contributor.author | Chao-Jung Cheng | en |
| dc.contributor.author | 鄭兆榮 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:07:18Z | - |
| dc.date.available | 2012-07-29 | |
| dc.date.copyright | 2010-07-29 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-26 | |
| dc.identifier.citation | 1
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46403 | - |
| dc.description.abstract | 本論文探討主題為: 測試新型材料:雙苯基化合物 (CB2C)應用於製作生物感測器的功效，並研究施加表面電場對於生物感測器的精度是否能達到有效提升的效果。實驗先以微機電製程，製作微米級的電晶體結構空晶片，接著在晶片上依序用不同的化合材料形成共價鍵以連接後續的生物分子，之後以不同濃度的抗體/抗原對鍵結於晶片之上，並以螢光實驗輔助觀察分子鍵結的情形，最後用自行架設的量測平台進行 電流測量。實驗的結果發現，新型化合材料CB2C應用於製作電晶體型的生物感測器時，比起傳統常用的Glutaraldehyde (戊二醛)擁有更佳的導電度，且具有受外加電場控制的特性。藉由此特性，可以放大通過晶片的電流，更容易觀察到電訊號的差異值，進而達到精度提升的效果。根據結果數據顯示，加上垂直電場時，比起不加垂直電場，所能測到的最高精度由原先的0.1 µg/ml∼1 µg/ml上升到0.1 µg/ml，另外也以S-100以及CRP兩種不同的抗體/抗原對進行相互比對，皆可觀察到同樣的效應。而本實驗結果也成功證實外加電場對生物感測器的精度能有效提升。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:07:18Z (GMT). No. of bitstreams: 1 ntu-99-R97525021-1.pdf: 2770238 bytes, checksum: d73e40a3c2032dca38bf3a3fa67fdecc (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 iii Abstract iv 目錄 v 圖目錄 vii 表目錄 x 第一章 緒論 - 1 - 1-1 前言 - 1 - 1-2 文獻回顧與介紹 - 2 - 1-2-1 生物感測器簡介 - 2 - 1-2-2 電化學生物感測器 - 3 - 1-2-2-1 基本理論 - 3 - 1-2-2-2 辨識分子與分析法 - 6 - 1-2-3 生物場效電晶體 - 10 - 1-2-4 總結比較 - 11 - 1-3 研究動機 - 13 - 1-4 論文架構 - 14 - 第二章 材料介紹及應用 - 15 - 2-1 實驗藥品 - 15 - 2-2 自組裝層材料 - 16 - 2-2-1 SAM自組裝層技術 - 16 - 2-2-2 3-氨基丙基三乙氧基硅烷(3APTES) - 17 - 2-2-3 雙苯基化合物CB2C介紹 - 19 - 2-3 活化與阻層材料 - 21 - 2-4 生物分子樣本 - 24 - 第三章 生物有機場效電晶體製作流程與量測 - 28 - 3-1 晶片結構 - 28 - 3-2 製作流程 - 29 - 3-3 生物分子固定化流程 - 37 - 3-4 量測儀器與方法 - 42 - 第四章 實驗結果與討論 - 51 - 4-1量測平台與商用儀器之比較 - 51 - 4-2 影響實驗之因素與改進方法 - 58 - 4-3實驗結果與比較 - 66 - 4-3-1 空片測試 - 66 - 4-3-2 3APTES與CB2C測試 - 68 - 4-3-3 活化與阻層後的影響 - 71 - 4-3-4 S100濃度測試 - 72 - 4-3-5 不同閘極電壓對實驗影響 - 76 - 4-3-6螢光實驗測試 - 79 - 4-3-7 PBS濃度與CB2C之效能探討 - 81 - 4-3-8 CRP與專一檢測的驗證 - 87 - 4-3-9機制討論 - 91 - 第五章 結論與未來展望 - 96 - 5-1 結論 - 96 - 5-2 未來展望 - 97 - 參考資料 - 98 - | |
| dc.language.iso | zh-TW | |
| dc.subject | CRP | zh_TW |
| dc.subject | 表面電場 | zh_TW |
| dc.subject | S100 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | S100 | en |
| dc.subject | Bio-sensor | en |
| dc.subject | surface electric field | en |
| dc.subject | CRP | en |
| dc.title | 應用雙苯基化合物於提升場效應生物分子感測技術之研究 | zh_TW |
| dc.title | The Implementation of Biphenyl Compound in Field-Effect Biomolecular Sensing Technology | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林致廷(Chih-Ting Lin) | |
| dc.contributor.oralexamcommittee | 李世光(Chih-Kung Lee),李世元(Shih-Yuan Lee) | |
| dc.subject.keyword | 生物感測器,表面電場,S100,CRP, | zh_TW |
| dc.subject.keyword | Bio-sensor,surface electric field,S100,CRP, | en |
| dc.relation.page | 100 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-27 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 工程科學及海洋工程學研究所 | zh_TW |
| 顯示於系所單位： | 工程科學及海洋工程學系 | |
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