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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蘇南維 | |
| dc.contributor.author | Yen-Lun Kung | en |
| dc.contributor.author | 龔彥綸 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:00:21Z | - |
| dc.date.available | 2012-07-30 | |
| dc.date.copyright | 2010-07-30 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-28 | |
| dc.identifier.citation | 王德男、李文立（2006）。木瓜產業研討會專刊。行政院農業委員會農業試驗所，台中，台灣。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46257 | - |
| dc.description.abstract | 本研究利用先前發表之酵素聯結親和吸附分析法（Food Chem. 2009, 113: 1218-1225），以聚丙烯醯胺聯結N-乙醯半乳糖胺（GalNAc）做為篩選凝集素之介質，發現木瓜子中含有與GalNAc專一結合之凝集素。檢驗不同成熟度之木瓜子與凝集素含量的關係，發現青木瓜中未成熟之白子具有較高的凝集素比活性。本研究依下列方式分離木瓜子凝集素：首先將樣品冷凍乾燥後磨碎過篩（40 mesh），以20倍（v/w）PBS buffer（pH 7.4）於4oC萃取隔夜，離心後取上清液加硫酸銨至70%飽和度，離心取沉澱回溶透析後，再經超過濾進行分子量區分。將分子量大於50 kDa的區分，以HiTrap CM FF進行離子交換層析及Superdex 200 GL膠體過濾區分後，分離得純化之凝集素（Carica papaya lectin, CPL）。SDS-PAGE結果顯示，於38及40 kDa分別有一明顯的色帶；Shodex KW-804膠體過濾層析顯示，原態CPL的分子量為804 ± 30 kDa，推測CPL應由α、β兩個次單元組成的多聚體。溫度及酸鹼安定性方面，CPL在70oC會失活，且於pH 6.0~8.0之間皆保有最高的活性。以EDTA和Ca2+、Mg2+、Mn2+、Zn2+處理CPL，發現其不需要二價金屬離子來維持活性。醣類抑制試驗結果指出，CPL對GalNAc有顯著特異性，其次是lactose。對人類不同血型紅血球的凝集能力結果顯示，CPL對A型血球具有較高的凝集活性。另外，以自行衍生的GalNAc-Sepharose 6B親和性管柱來純化CPL，可提高其純度達粗萃液的6000倍。 | zh_TW |
| dc.description.abstract | Based on our previous study, a novel N-acetylgalactosamine binding protein in papaya seed (Carica papaya lecin, CPL) was discovered by GalNAc-polyacrylamide based enzyme-linked adsorbent assay (Food Chem. 2009, 113: 1218-1225). Papaya seeds with different maturity were collected, and the extracts of seeds in green papaya fruits shows relatively high activity. Further purification of CPL was conducted as following: freeze-dried papaya seeds were ground into powder, extracted with 20 folds (w/v) of 50 mM phosphate buffered saline (pH 7.4) at 4°C overnight, centrifuged and collected the supernatant, added ammonium sulfate to 70% saturation. Precipitations were resuspended and dialyzed against PBS, then fractionated by a 50 kDa MWCO ultrafiltration. The retentate was further purified by HiTrap CM FF ion exchange chromatography and Superdex 200 GL gel filtration chromatography. SDS-PAGE and HPLC gel filtration indicated that CPL is a polymer with a molecular mass of 804 ± 30 kDa and composed of two different subunits of 38 and 40 kDa associated by non-covalent bonds. It was heat stable until up to 70oC for 30 min and showed optimum sugar-binding activity from pH 6.0 to 8.0. Also, CPL did not require Ca2+, Mg2+, Mn2+, Zn2+for its activity. Of various sugars tested, the lectin was best inhibited by GalNAc. CPL agglutinated all trypsinized human RBC types, with a slight preference for the A blood group which immunodeterminant is GalNAc. Based on the specificity, we performed an alternative ripid, simple purification method via GalNAc-Sepharose 6B affinity chromatography column, and gave the lectin with a 6000-fold purification as compared to the crude extract. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:00:21Z (GMT). No. of bitstreams: 1 ntu-99-R97623007-1.pdf: 27575592 bytes, checksum: a3eee534a6e7b42b8bedfc7379961d30 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目錄 I
表目錄 VI 縮寫表 VII 中文摘要 X 英文摘要 XI 第一章 前言 1 第二章 文獻回顧 3 第一節 凝集素之介紹 3 1. 凝集素之定義與特性 3 2. 凝集素之研究發展簡史 5 3. 凝集素與醣類結合之機制 8 4. 凝集素之檢測 9 5. 凝集素之來源與分類 11 5.1 植物來源凝集素（Plant lectins） 11 5.2 動物來源凝集素（Animal lectins） 14 5.3 其他來源凝集素 17 6. 植物凝集素於自然界之功能 18 6.1 凝集素可做為植物天然的防禦蛋白 18 6.2 凝集素參與植物和固氮細菌的共生作用 20 7. 植物凝集素之應用 21 7.1 植物凝集素做為分析工具 21 7.2 植物凝集素可刺激細胞增生 22 7.3 植物凝集素用於疾病診斷與治療 22 7.4 植物凝集素的其他應用 24 第二節 番木瓜簡介 26 第三節 番木瓜種子之相關研究 29 1. 木瓜子中之異硫氰酸苄酯（Benzyl isothiocyanate, BITC） 29 2. 木瓜子萃取物對生殖能力之影響 31 第三章 材料與方法 32 第一節 實驗大綱 32 第二節 實驗材料 32 第三節 實驗儀器 35 第四節 實驗方法 36 1. 不同成熟度木瓜子之凝集素活性檢測 36 1.1 樣品前處理與凝集素活性追蹤 36 1.2 統計分析 36 2. 木瓜子凝集素之純化與生化特性分析 37 2.1 凝集素之分離與純化 37 2.2 蛋白質含量測定 38 2.3 凝集素活性追蹤 38 2.4 膠體電泳 40 2.5 熱安定性試驗 40 2.6 酸鹼值對凝集素活性之影響 40 2.7 還原劑對凝集素活性之影響 41 2.8 二價金屬離子對凝集素活性之影響 41 2.9 醣類抑制試驗 41 2.10 LC-MS/MS 42 3. 利用親和性膠體純化木瓜子凝集素 43 3.1 親和性膠體製備 43 3.2 親和性管柱層析 43 3.3 膠體過濾管柱層析 44 第四章 結果與討論 45 第一節 不同成熟度木瓜子之凝集素活性檢測 45 第二節 木瓜子凝集素之純化與生化特性分析 49 1. 木瓜子凝集素之分離與純化 49 2. 膠體電泳與分子量測定 50 3. 熱處理、酸鹼值及還原劑對凝集素活性之影響 51 4. 二價金屬離子對凝集素活性之影響 51 5. 醣類抑制試驗 51 6. 不同紅血球之凝集狀況分析 52 7. LC-MS/MS 54 第三節 利用親和性膠體純化木瓜子凝集素 72 第五章 結論 79 第六章 參考文獻 80 附錄一 90 附錄二 91 附錄三 92 | |
| dc.language.iso | zh-TW | |
| dc.title | 番木瓜種子凝集素之純化與生化特性探討 | zh_TW |
| dc.title | Purification and Biochemical Properties of an N-acetylgalactosamine Specific Lectin from Papaya (Carica papaya) Seeds | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李敏雄,許輔,林銘澤,古國隆 | |
| dc.subject.keyword | 番木瓜,乙醯半乳糖胺,親醣蛋白,凝集素, | zh_TW |
| dc.subject.keyword | papaya,N-acetylgalactosamine,suger-binding protein,lectin, | en |
| dc.relation.page | 92 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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