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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周崇熙 | |
dc.contributor.author | Shr-Fong Jiang | en |
dc.contributor.author | 蔣世峯 | zh_TW |
dc.date.accessioned | 2021-06-15T04:54:22Z | - |
dc.date.available | 2015-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-30 | |
dc.identifier.citation | 林正忠,2008。台灣上市期豬、雞沙氏桿菌之分離率、血清型分佈及抗藥性研究。碩士論文。國立中興大學獸醫學系暨研究所。
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Infect Immun. 69(12):7873-9. Wilson IG, 2002, Salmonella and Campylobacter contamination of raw retail chickens from different producers: a six year survey. Epidemiol Infect. 129(3):635-45. Yan SS, Pendark ML, Abela-Ridder B, Punderson JW, Fedorko DP, Foley SL, 2004, An overview of Salmonella typing Public health perspectives. Applied Immunol Rev. 4: 189-204. Yu CY, Chu C, Chou SJ, Chao MR, Yeh CM, Lo DY, Su YC, Horng YM, Weng BC, Tsay JG, Huang KC, 2008, Comparison of the association of age with the infection of Salmonella and Salmonella enterica Serovar Typhimurium in Pekin ducks and Roman geese. Poult Sci. 87(8):1544-9. Zhao C, Ge B, De Villena J, Sudler R, Yeh E, Zhao S, White DG, Wagner D, Meng J, 2001, Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the greater Washington, DC area. Appl Environ Microbiol. 67(12):5431-6. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46113 | - |
dc.description.abstract | 沙門氏菌是造成人類食因病的重要病因之一,經常藉由食入受汙染的禽肉與禽製品感染。本調查的目的是希望能找出並釐清臺灣白肉種雞場之汙染模式,以有助於政府對白肉種雞場進行的監測計畫與沙門氏菌控制計畫之研擬。本研究針對臺灣地區十四場白肉種雞場自其一日齡雛雞進雛起進行縱向性長期觀測,樣本總共2132個,包括一日齡雛雞(只有11場從一日齡雛雞開始監測)、沾有墊料和糞便的鞋套、飼料、水樣、中止蛋與孵化出之子代小雞,並依樣本種類不同共分成為1080個檢驗樣本,總分離率為8.3% (90/1080);其中一日齡雛雞之分離率為24.6% (15/61);沾有墊和料糞便的鞋套之分離率為13.1% (52/398);飼料之分離率為5.5% (16/292);水樣之分離率為0% (0/133);中止蛋與孵化出之子代小雞之分離率為3.6% (7/196)。在這14場白肉種雞場中只有12場能從一日齡雛雞與鞋套樣本中分離到沙門氏菌,場陽性率為85.7%(12/14),其中以S. Albany、S. Duesseldorf與S. Schwarzengrund等血清型最常被分離到,同時利用脈衝式電泳之分子分型以將分離株進行親緣性關係鑑定。從本次調查的結果發現在臺灣地區的白肉種雞場中,以一日齡雛雞是白肉種雞場內重要的沙門氏菌感染來源,而由飼料樣本分離到之沙門氏菌血清型與基因型來看,發現飼料雖然有被沙門氏菌汙染的情形出現,但造成白肉種雞場內持續傳播感染的機率卻不高。並且同一基因型的沙門氏菌在感染後可以持續於雞場內傳播,並可能經由垂直傳播模式傳給子代的商用白肉雞,因此在選購沙門氏菌清淨的小種雞是很重要的關鍵。另外在本次調查中亦發現白肉種雞場的種雞飼育隻數、飼育設備與白肉種雞場之沙門氏菌感染關係不大。 | zh_TW |
dc.description.abstract | Salmonella is one of the important causes of human food disease, and people were often infected through ingestion of contaminated poultry and poultry products. The purpose of this study is to identify and clarify the pollution patterns of broiler breeder farms in Taiwan, and help the government carry out the monitor plan and develop the Salmonella control plan of broiler breeder farms. In this study, 14 broiler breeder farms in Taiwan were investigated by longitudinal observation since the first days of the 1-day-old chicken. The total numbers of samples were 2132, including 1-day-old chicken (there are only 11 farms sent samples of 1-day-old chichken), shoe covers stained with litter and feces, feed, water, suspended eggs and hatched chicken, and was divided into 1080 test samples according to the different types of samples. The total isolation rate was 8.3% (90/1080); the isolation rate of 1-day-old chicken was 24.6% (15/61); the isolation rate of shoe covers stained with litter and feces was 13.1% (52/398); the isolation rate of feed was 5.5% (16/292); the isolation rate of water was 0% (0/133); the isolation rate of suspended eggs and hatched chicken was 3.6% (7/196). In the 14 breeder farms, Salmonella were isolated from 12 farms, and the farm positive rate was 85.7%(12/14). In this study, S. Albany, S. Duesseldorf, and S. Schwarzengrund were the most frequently isolated serotypes and we used pulsed field gel electrophoresis to identify the genetic relationships of Salmonella isolates. These results showed that samples of 1-day-old chicken are important sources of Salmonella infection and though feed can be contaminated by Salmonella, the risk of Salmonella infection of chicken from feed is not very high. The Salmonella of the same genotype can persistently transmit in the broiler breeder farm, and may transmit to the child commercial broiler through vertical transmittion. Therefore, it is an important key to buy Salmonella free broiler breeder chicks. In this study we also found that the relationship of Salmonella infection between the numbers of broiler breeder and the raising facilities of broiler breeder farms is not close. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:54:22Z (GMT). No. of bitstreams: 1 ntu-99-R97629037-1.pdf: 1929974 bytes, checksum: 36ae9e581bc7f9760f54e2055e3baa20 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目次
致謝………………………………………………………………………Ⅰ 中文摘要…………………………………………………………………Ⅲ Abstract…………………………………………………………………. Ⅴ 目次………………………………………………………………………Ⅶ 表次………………………………………………………………………Ⅹ 圖次……………………………………………………………………ⅩⅠ 第一章 緒言..……………………………………………………………1 第二章 文獻探討………………………………………………………..4 第一節 沙門氏菌簡介…………..………………………………4 第二節 沙門氏菌流行病學……………………………………..8 第三節 雞隻之沙門氏菌感染情形……………………………11 第四節 沙門氏菌之培養與分離………………………………16 第五節 脈衝式電泳……………………………………………20 第六節 縱向性研究……………………………………………24 第七節 危險因子………………………………………………27 第三章 材料與方法……………………………………………………29 第一節 樣本採集………………………………………………29 第二節 沙門氏菌之前增菌培養..……………………………..30 2-1 一日齡小雞………………………………………..30 2-2 鞋套………………………………………………..30 2-3 飼料………………………………………………..30 2-4 水質樣本…………………………………………..31 2-5 中止蛋……………………………………………..31 2-6 產蛋孵化之子代小雞……………………………..31 第三節 沙門氏菌之分離與鑑定………………………………33 3-1 沙門氏菌之分離…………….……………………..33 3-2 O抗原測定………………………………………...34 3-3 H抗原測定………………………………………...34 第四節 脈衝式電泳……………………………………………35 4-1 菌株與試劑製備…………………………………...36 4-2 製作與清洗PFGE菌塊……………………………36 4-3 限制酶之切割……………………………………...37 4-4 PFGE結果…………………………………………38 第四章 實驗結果………………………………………………………39 第一節 沙門氏菌分離率………………………………………39 第二節 沙門氏菌血清型………………………………………42 第三節 沙門氏菌脈衝式電泳之分子生物學分型……………47 第五章 討論……………………………………...…………………….51 第一節 樣本之分離率討論…………………………………...51 第二節 分離株之血清型討論………………………………...57 第三節 分離株之分子生物學分型討論……………………...59 第四節 臺灣地區白肉種雞場沙門氏菌感染之危險因子……69 第六章 結論……………………………………………………………70 第七章 參考文獻………………………………………………………72 表格……………………………………………………………………….80 圖片……………………………………………………………………….90 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣地區白肉種雞場之沙門氏菌汙染模式研究 | zh_TW |
dc.title | The investigation of Salmonella contamination model in broiler breeder farms in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡向榮,張紹光,連一洋,陳秋麟 | |
dc.subject.keyword | 沙門氏菌,白肉種雞場,汙染模式,縱向性研究,脈衝式電泳,危險因子, | zh_TW |
dc.subject.keyword | Salmonella,broiler breeder farm,contamination model,longitudinal study,pulsed field gel electrophoresis,risk factor, | en |
dc.relation.page | 101 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-30 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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