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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李篤中 | |
dc.contributor.author | Fa-Chin Liu | en |
dc.contributor.author | 劉法志 | zh_TW |
dc.date.accessioned | 2021-06-15T04:46:47Z | - |
dc.date.available | 2013-08-09 | |
dc.date.copyright | 2010-08-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-04 | |
dc.identifier.citation | A. Evren Tugtas, Spyros G. Pavlostathis. ( 2007) , Effect of Sulfide on Nitrate Reduction in Mixed Methanogenic Cultures. Biotechology and Bioengineering, Vol. 97, No. 6,.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45823 | - |
dc.description.abstract | 本文測試異營反硝化菌Pseudomonas sp. C27在不同碳源、溫度、酸鹼、初始硫化物濃度、溶氧量下同步去除硫、氮及碳之效率,並進行連續式反應器的操作。
結果顯示此菌種最容易利用之碳源為Acetate,適合在溫度25~30℃與pH為9~9.5的環境中生長。不同初始硫化物濃度會影響硫化物最終產物型態,在初始NO3--N為105mg/L、硫化物濃度小於100mg/L時,硫化物會被先氧化至Sulfur,Sulfur會進一步被殘餘的Nitrate轉化成Thiosulfate,而當Sulfide被完全氧化後異營反硝化作用才會進行,以過量Acetate將剩餘的Nitrate與Nitrite,在初始Sulfide濃度100mg/L以下時完全反硝化為N2。當初始Sulfide濃度達到200mg/L以上時反硝化最終產物為NO2-而非N2。在微氧條件下(DO=0~10%)反應遲滯期較厭氧條件下縮短了48小時,且能夠進行完全反硝化,Sulfide Oxidase、Nitrate Reductase及Nitrite Reductase活性在微氧條件皆高於厭氧條件下,但繼續提高溶氧量則此效果降低。 在連續式反應器中Pseudomonas sp. C27在微氧條件下可在Sulfide、NO3--N、Ac-C負荷量分別為0.215kg/m3day、0.146kg/m3day、0.092 kg/m3day達到84.7%、99.5%及74.1%之移除率。 使用Pseudomonas sp. C27進行同步脫硫反硝化程序不需仰賴其它菌種協同作用共生完成,與生長速度快,為新型的脫硫反硝化程序。 | zh_TW |
dc.description.abstract | Denitrification is a critical step in biological wastewater treatment process since nitrogen reduction is easily affected by various conditions. The complicated compositions in the wastewater (such as types of carbon source, sulfide concentration) and reaction conditions (e.g. temperature, pH, dissolved oxygen) affect efficiency and pathways of the denitrifying microbial dissimilation. Previous studies are not shown clear statements on related mechanisms and constrains. As a result, this study performed a modified denitrfication process, namely simultaneous denitrification and sulfide degradation (SDSD), via one unique denitrfier, Pseudomonas sp. C27. This heterotrophic species was isolated from an expanded granular sludge bed (EGSB) reactor and is reported more efficient than autotrophic species.
Experiments are carried out in a tailor-made continuous-flow stirred reactor (CSTR). Composition of feed, i.e. various carbon sources (including alcohol, organic acid and carbonhydrate), and initial sulfide concentration (0, 50, 96.1, 193.5, 302.1 and 404.6 mg.L-1), are prepared in the lab and tested SDSD efficiency. In the meanwhile, effects of operational conditions, pH (6.5, 7.0, 8.5, 9.0, 9.5, 10.15 and 11) and temperature (20, 25, 30, 35, 40, 45℃), on SDSD efficiency are investigated. Results are shown that the optimum conditions for this strain to achieve SDSD are at 25~30℃ and pH 9~9.5. The most easy assimilated carbon source for Pseudomonas sp. C27 is acetate although other smaller molecular organics (such as succinate and malate) can be biodegraded within 24 hours. The sulfide and acetate were used as electron donors, and the end product of sulfide oxidation was sulfur or thiosulfate by the NO3-N/S2—S molar ratios. Moreover, products of nitrate reduction were determined by the initial sulfide concentrations, when the initial sulfide concentrations were set below 96.1mg/L, the nitrate was conveted to nitrogen gas; Moreover, the sulfide concentration reached 193.5mg/L, the nitrite reductase was strongly inhibited by sulfide casuing the main end product of denitrification was nitrite. When the sulfide concentration was as high as 302.1mg/L, the isolate C27 didn’t display growth and the ability of denitrification. In micro-oxygen condition (DO=0~10%) the growth time of bacterium was shorted 48 hours compare with anaerobic condition, because of the specify activity of enzymes were higher than anaerobic condition. Testing in a CSTR at sustainable loadings of 0.215 kg m-3day-1 for sulfide, 0.146 kg m-3day-1 for nitrate-nitrogen , 0.092 kg m-3day-1 for acetate-carbon with 84.7%, 99.5%, 74.1% efficiency respectively. Mass balance of sulfur are calculated to facilitate analyzing microbial preferred reactions and possible SDSD pathways for Pseudomonas sp. C27 are suggested in the end of this thesis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:46:47Z (GMT). No. of bitstreams: 1 ntu-99-R97524071-1.pdf: 1202147 bytes, checksum: b74f2a5cb4330c1a7e88baaa84a9ea46 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 致謝………..……………………………………………………………………………...I
中文摘要………………………………………………………………………………....II Abstract………………………………………………………………………………...III 目錄 V 圖目錄 VIII 表目錄 XI 第一章 前言 1 第二章 文獻回顧 2 2-1 含硫含氮有機廢水的危害 2 2-2 含硫廢水生物處理技術 2 2-2-1 硫化物生物氧化程序 2 2-2-2 利用Achromatiaceae的硫化物氧化程序 3 2-2-3 利用光合硫细菌的硫氧化程序 3 2-3 含氮廢水生物處理技術研究現況 4 2-3-1 短程硝化反硝化 6 2-3-2 同時硝化反硝化 6 2-3-3 好氧反硝化 7 2-3-4 限氧自營硝化—反硝化 7 2-3-5 藻類養殖脱氮 7 2-4 生物同步脫硫脫氮技術 8 2-4-1 自營硫氧化反硝化 8 2-4-2 自營/異營反硝化聯合生物技術 10 2-4-3 同步脱硫反硝化程序 11 2-5 同步去除硫、氮、碳研究 12 2-6 本研究目的 15 第三章 實驗材料與方法 16 3-1 實驗材料 16 3-1-1 微生物 16 3-1-2 培養基組成 17 3-2 實驗設計 18 3-2-1 碳源測試 18 3-2-2 酸鹼值實驗 20 3-2-3 溫度實驗 20 3-2-3 硫化物濃度實驗 21 3-2-4 含氧量實驗 21 3-2-5 連續流反應器 23 3-3 實驗方法 23 3-3-1 硫化物測定 23 3-3-2 氨氮測定 26 3-3-3 蛋白質測定 28 3-3-4 質量平衡計算單質硫產率 30 3-3-4 生物產氣及氮氣測定 30 3-3-5 陰離子濃度分析 32 3-3-6 酸鹼值(pH)測定 35 3-3-7 發酵槽操作 35 3-3-8 酵素比活性測定 36 第四章 結果與討論 44 4-1 碳源測試 44 4-2 酸鹼測試 46 4-3 溫度測試 56 4-4 不同硫化物濃度之影響 65 4-4-1 控制對照組 65 4-4-2 初始硫化物濃度193.5mg/L測試 65 4-4-3 自營培養與異營培養 67 4-4-4 初始硫化物濃度50mg/L測試 70 4-4-5 初始硫化物濃度96.1mg/L測試 72 4-4-6 初始硫化物濃度302.1mg/L測試 74 4-4-7 初始硫化物濃度404.6mg/L測試 76 4-4-8 產氣情形 78 4-4-9 不同初始硫化物下對Psedomonas sp. C27代謝之推導 79 4-5 不同含氧量(DO)實驗 88 4-5-1 厭氧(DO=0%)與微氧(DO<10%)實驗的比較 88 4-5-2 高溶氧量實驗 95 4-5-3 以氧氣作為電子受體 104 4-5-4 初始硫化物濃度310.2mg/L在微氧條件下效果 108 4-6 連續式反應器操作 111 第五章 結論 119 第六章 參考文獻 120 | |
dc.language.iso | zh-TW | |
dc.title | 以異營反硝化菌Pseudomonas sp. C27進行同步脫硫反硝化 | zh_TW |
dc.title | Use of heterotrophic denitrifier Pseudomonas sp. C27 to achieve simultaneous removal of sulfide, nitrate and organic carbon | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 周志雄,朱曉萍,黃志彬,劉志成,張嘉修 | |
dc.subject.keyword | 同步脫硫反硝化, | zh_TW |
dc.subject.keyword | Simultaneous denitrifying sulfide removal, | en |
dc.relation.page | 128 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-05 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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