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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蕭信宏 | |
| dc.contributor.author | Chun-Hao Huang | en |
| dc.contributor.author | 黃俊豪 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:46:12Z | - |
| dc.date.available | 2012-09-09 | |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-05 | |
| dc.identifier.citation | Alphey, L. (2009). Natural and engineered mosquito immunity. J Biol 8, 40.
An, C., Jiang, H., and Kanost, M.R. (2010). Proteolytic activation and function of the cytokine Spätzle in the innate immune response of a lepidopteran insect, Manduca sexta. FEBS J 277, 148-162. Arnot, C.J., Gay, N.J., and Gangloff, M. (2010). Insights into the molecular mechanism that induces activation of Spätzle, the ligand for the Drosophila Toll receptor. J Biol Chem M109.098186. Arnot, C.J., Gay, N.J., and Gangloff, M. (2010). Molecular mechanism that induces activation of Spätzle, the ligand for the Drosophila Toll receptor. J Biol Chem 285, 19502-19509. Attardo, G.M., Hansen, I.A., and Raikhel, A.S. (2005). Nutritional regulation of vitellogenesis in mosquitoes: implications for anautogeny. Insect Biochem Mol Biol 35, 661-675. Attardo, G.M., Hansen, I.A., Shiao, S.H., and Raikhel, A.S. (2006). Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction. J Exp Biol 209, 3071-3078. Bian, G., Raikhel, A.S., and Zhu, J. (2008). Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito. Insect Biochem Mol Biol 38, 190-200. Bischoff, V., Vignal, C., Boneca, I.G., Michel, T., Hoffmann, J.A., and Royet, J. (2004). Function of the drosophila pattern-recognition receptor PGRP-SD in the detection of Gram-positive bacteria. Nat Immunol 5, 1175-1180. Bownes, M. (1986). Expression of the genes coding for vitellogenin(yolk protein). Ann Rev Entomol 31,507-531. Cerione, R.A. (2004). Cdc42: new roads to travel. Trends Cell Biol 14, 127-132. Costa, A., Jan, E., Sarnow, P., and Schneider, D. (2009). The Imd pathway is involved in antiviral immune responses in Drosophila. PLoS One 4, e7436. Daopin, S., Piez, K.A., Ogawa, Y., and Davies, D.R. (1992). Crystal structure oftransforming growth factor-beta 2: an unusual fold for the superfamily. Science 257,369-373. Dhadialla, T.S., Raikhel, A.S. (1994). Endocrinology of mosquito vitellogenesis. In: Perspectives in Comparative Endocrinology. National Research Council of Canada, Canada 275-281. Gangloff, M., Murali, A., Xiong, J., Arnot, C.J., Weber, A.N., Sandercock, A.M., Robinson, C.V., Sarisky, R., Holzenburg, A., Kao, C., et al. (2008). Structural insight into the mechanism of activation of the Toll receptor by the dimeric ligand Spätzle. J Biol Chem 283, 14629-14635. Gay, N.J., Gangloff, M., and Weber, A.N. (2006). Toll-like receptors as molecular switches. Nat Rev Immunol 6, 693-698. Gillies, M.T. (1955). The pre-gravid phase of ovarian development in anophelesfunestus. Ann Trop Med Parasitol 49, 320-325. Gordon, M.D., Dionne, M.S., Schneider, D.S., and Nusse, R. (2005). WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity. Nature 437, 746-749. Gottar, M., Gobert, V., Matskevich, A.A., Reichhart, J.M., Wang, C., Butt, T.M., Belvin, M., Hoffmann, J.A., and Ferrandon, D. (2006). Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors. Cell 127, 1425-1437. Hagedorn, H.H. (1985). The role of ecdysteroids in reproduction. In: Kerkut, G., Gilbert, L.(Eds.), Comprehensive Insect Physiology. Biochemistry and Pharmacology8, 205-261. Hagedorn, H.H. (1989). Physiological roles of hemolymph ecdysteroid in the adult insect. In: Koolman, J.(Ed.), Ecdysone from Chemistry to Mode of Action. New York, Thieme Medical Publishers, Inc, 79-289 Hansen, I.A., Attardo, G.M., Roy, S.G., and Raikhel, A.S. (2005). Target of rapamycin-dependent activation of S6 kinase is a central step in the transduction of nutritional signals during egg development in a mosquito. J Biol Chem 280, 20565-20572. Hansen, I.A., Geoffrey, M.A., Park, J.H., Peng, Q., Raikhel, A.S. (2004). Target of rapamycin-mediated amino acid signaling in mosquito anautogeny. PNAS 101(29),10626-10631. Hoffmann, A., Funkner, A., Neumann, P., Juhnke, S., Walther, M., Schierhorn, A.,Weininger, U., Balbach, J., Reuter, G., and Stubbs, M.T. (2008). Biophysicalcharacterization of refolded Drosophila Spätzle, a cystine knot protein, revealsdistinct properties of three isoforms. J Biol Chem 283, 32598-32609. Hoffmann, J.A., Kafatos, F.C., Janeway, C.A., and Ezekowitz, R.A. (1999). Phylogenetic perspectives in innate immunity. Science 284, 1313-1318. Jame, S.P., Mizuguchi, K., Gay, N.J. (2001). A Family of Proteins Related to Spätzle,the Toll Receptor Ligand, Are Encoded in the Drosophila Genome.PROTEINS:Structure, Function, and Genetics 45(1), 71-80. Jang, I.H., Chosa, N., Kim, S.H., Nam, H.J., Lemaitre, B., Ochiai, M., Kambris, Z.,Brun, S., Hashimoto, C., Ashida, M., et al. (2006). A Spätzle-processing enzyme required for toll signaling activation in Drosophila innate immunity. Dev Cell 10, 45-55. Jiang, R., Kim, E.H., Gong, J.H., Kwon, H.M., Kim, C.H., Ryu, K.H., Park, J.W., Kurokawa, K., Zhang, J., Gubb, D., et al. (2009). Three pairs of protease-serpin complexes cooperatively regulate the insect innate immune responses. J Biol Chem 284, 35652-35658. Kambris, Z., Brun, S., Jang, I.H., Nam, H.J., Romeo, Y., Takahashi, K., Lee, W.J.,Ueda, R., and Lemaitre, B. (2006). Drosophila immunity: a large-scale in vivo RNAiscreen identifies five serine proteases required for Toll activation. Curr Biol 16,808-813. Kategaya, L.S., Changkakoty, B., Biechele, T., Conrad, W.H., Kaykas, A., Dasgupta, R., and Moon, R.T. (2009). Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6. PLoS One 4, e6129. Kurata, S. (2001). Extracellular and intracellular pathogen recognition by Drosophila PGRP-LE and PGRP-LC. Int Immunol 22, 143-148. Lan, Q., Grier, C.A. (2004). Critical period for pupal commitment in the yellow fever mosquito, Aedes aegypti. J Insect Physiol 50(7), 667-676 Lemaitre, B., and Hoffmann, J. (2007). The host defense of Drosophila melanogaster. Annu Rev Immunol 25, 697-743. Levashina, E.A., Langley, E., Green, C., Gubb, D., Ashburner, M., Hoffmann, J.A., and Reichhart, J.M. (1999). Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila. Science 285, 1917-1919. Li, C., Kapitskaya, M.Z., Zhu, J., Miura, K., Segraves, W., and Raikhel, A.S. (2000). Conserved molecular mechanism for the stage specificity of the mosquito vitellogenic response to ecdysone. Dev Biol 224, 96-110. Michel, T., Reichhart, J.M., Hoffmann, J.A., and Royet, J. (2001). Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognitionprotein. Nature 414, 756-759. Neil, W.I. (1995). Cystine knots. Structural Biology 5,391-395. O’Meara, G.F. (1979). Variable expressions of autogeny in three mosquito species. Int J Invert Repro 1,253-261. Oefner, C., D'Arcy, A., Winkler, F.K., Eggimann, B., and Hosang, M. (1992). Crystal structure of human platelet-derived growth factor BB. EMBO J 11, 3921-3926. Pal, S., and Wu, L.P. (2009). Pattern recognition receptors in the fly: lessons we can learn from the Drosophila melanogaster immune system. Fly (Austin) 3, 121-129.Pierceall, W.E., Li, C., Biran, A., Miura, K., Raikhel, A.S., and Segraves, W.A. (1999). E75 expression in mosquito ovary and fat body suggests reiterative use of ecdysone-regulated hierarchies in development and reproduction. Mol Cell Endocrinol 150, 73-89. Raikhel, A.S., and Dhadialla, T.S. (1992). Accumulation of yolk proteins in insect oocytes. Annu Rev Entomol 37, 217-251. Raikhel, A.S., Kokoza, V.A., Zhu, J., Martin, D., Wang, S.F., Li, C., Sun, G., Ahmed, A., Dittmer, N., Attardo, G. (2002). Molecular biology of mosquito vitellogenesis: from basic studies to genetic engineering of antipathogen immunity. Insect Biochem Mol Biol 32(10),1275-1286. Raikhel, A.S., Miura, K., Segraves, W.A. (1999). Nuclear receptors in mosquitovitellogenesis. Amer Zoologist 39(4),722-735. Rose, W.C. (1938). The nutritive significance of the amino acids. Physiol Rev18,109-136. Ryu, J.H., Ha, E.M., and Lee, W.J. Innate immunity and gut-microbe mutualism inDrosophila. Dev Comp Immunol 34, 369-376. Schlunegger, M.P., and Grutter, M.G. (1992). An unusual feature revealed by the crystal structure at 2.2 A resolution of human transforming growth factor-beta 2. Nature 358, 430-434. Segraves, W.A., and Hogness, D.S. (1990). The E75 ecdysone-inducible generesponsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily. Genes Dev 4, 204-219. Shi, X.Z., Zhang, R.R., Jia, Y.P., Zhao, X.F., Yu, X.Q., and Wang, J.X. (2009). Identification and molecular characterization of a Spätzle-like protein from Chinese shrimp (Fenneropenaeus chinensis). Fish Shellfish Immunol 27, 610-617. Shiao, S.H., Hansen, I.A., Zhu, J., Sieglaff, D.H., and Raikhel, A.S. (2008). Juvenile hormone connects larval nutrition with target of rapamycin signaling in the mosquito Aedes aegypti. J Insect Physiol 54, 231-239. Shiao, S.H., Whitten, M.M., Zachary, D., Hoffmann, J.A., and Levashina, E.A. (2006).Fz2 and cdc42 mediate melanization and actin polymerization but are dispensable for Plasmodium killing in the mosquito midgut. PLoS Pathog 2, e133. Shin, S.W., Bian, G., and Raikhel, A.S. (2006). A toll receptor and a cytokine, Toll5A and Spz1C, are involved in toll antifungal immune signaling in the mosquito Aedes aegypti. J Biol Chem 281, 39388-39395. Singh, K.R.P., Brown, A.W.A. (1957). Nutritional requirements of Aedes aegypti. J Insect Physiol 1,199-220. Takken, W., Klowden, M.J., and Chambers, G.M. (1998). Effect of body size on host seeking and blood meal utilization in Anopheles gambiae sensu stricto (Diptera: Culicidae): the disadvantage of being small. J Med Entomol 35, 639-645. Tolle, M.A. (2009). Mosquito-borne diseases. Curr Probl Pediatr Adolesc Health Care 39, 97-140. Uchida, K. (1998). Role of nutrition in initiation and promotion of ovarian development in the Japanese house mosquito, Culex pipiens pallens. Med Entomol Zool 49(2),75-85 Wang, H.Y., Liu, T., and Malbon, C.C. (2006). Structure-function analysis of Frizzleds. Cell Signal 18, 934-941. Wang, S.F., Li, C., Sun, G., Zhu, J., and Raikhel, A.S. (2002). Differential expression and regulation by 20-hydroxyecdysone of mosquito ecdysteroid receptor isoforms A and B. Mol Cell Endocrinol 196, 29-42. Wang, Y., Cheng, T., Rayaprolu, S., Zou, Z., Xia, Q., Xiang, Z., and Jiang, H. (2007). Proteolytic activation of pro-Spätzle is required for the induced transcription of antimicrobial peptide genes in lepidopteran insects. Dev Comp Immunol 31, 1002-1012. Wang, Y., and Zhu, S. (2009). Evolutionary and functional epitopes of the Spätzle protein: new insights into activation of the Toll receptor. Cell Mol Life Sci 66, 1595-1602. Waterhouse, R.M., Kriventseva, E.V., Meister, S., Xi, Z., Alvarez, K.S., Bartholomay, L.C., Barillas-Mury, C., Bian, G., Blandin, S., Christensen, B.M., et al. (2007). Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes. Science 316, 1738-1743. Weber, A.N., Tauszig-Delamasure, S., Hoffmann, J.A., Lelievre, E., Gascan, H., Ray, K.P., Morse, M.A., Imler, J.L., and Gay, N.J. (2003). Binding of the Drosophila cytokine Spätzle to Toll is direct and establishes signaling. Nat Immunol 4, 794-800. Xi, Z., Ramirez, J.L., and Dimopoulos, G. (2008). The Aed es aegypti toll pathwaycontrols dengue virus infection. PLoS Pathog 4, e1000098. Zhu, B., Pennack, J.A., McQuilton, P., Forero, M.G., Mizuguchi, K., Sutcliffe, B., Gu, C.J., Fenton, J.C., and Hidalgo, A. (2008). Drosophila neurotrophins reveal a common mechanism for nervous system formation. PLoS Biol 6, e284. Zhu, J., Busche, J.M., and Zhang, X. Identification of juvenile hormone target genes in the adult female mosquitoes. Insect Biochem Mol Biol 40, 23-29. Zhu, J., Chen, L., and Raikhel, A.S. (2003). Posttranscriptional control of the competence factor betaFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A 100, 13338-13343. Zhu, J., Chen, L., and Raikhel, A.S. (2007). Distinct roles of Broad isoforms in regulation of the 20-hydroxyecdysone effector gene, Vitellogenin, in the mosquito Aedes aegypti. Mol Cell Endocrinol 267, 97-105. Zou, Z., Shin, S.W., Alvarez, K.S., Kokoza, V., Raikhel, A.S. (2010). Distinct melanization pathways in the mosquito Aedes aegypti. Immunity 32(1),41-53. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45791 | - |
| dc.description.abstract | 蚊子是許多傳染性疾病的重要媒介，例如瘧疾、登革熱、絲蟲病以及日本腦炎…等疾病。針對這些病媒蚊傳播的疾病，各國的研究團隊除了致力於發展新藥及開發疫苗之外，以遺傳工程技術控制病媒傳病能力的新策略也漸漸受到重視。因此，研究蚊子體內對抗外來病原的相關基因及調控機制能提供此一策略新的目標。本實驗室之前的研究發現埃及斑蚊Inhibitor of Apoptosis-2(IAP2)參與病媒蚊抗菌蛋白合成之調控，而利用岡比亞瘧蚊(Anopheles gambiae)的cDNA microarray分析結果發現，當岡比亞瘧蚊被混和的細菌(包含Staphylococcus aureus 和Escherichia coli)或Plasmodium berghei 誘發免疫反應時，有多個免疫反應基因會隨著IAP2 表現量增加而大量表現，其中我們發現Spätzle-6(Spz6)的功能有待深入探討。在果蠅(Drosophila melanogaster)的研究顯示，果蠅的Toll pathway 可以藉由Spz 活化Toll receptor，進而促進Toll pathway 的訊號傳遞，引發抗菌蛋白的產生。同時，果蠅的Spz6 也被證實參與早期胚胎發育。因此，我們想更進一步研究Spz6 在埃及斑蚊的免疫反應及發育所扮演的角色。首我們藉由RNAinterference(RNAi)的方式來抑制埃及斑蚊Spz6 的表現，再分別去感染Beauveriabassiana, Staphylococcus aureus 和Escherichia coli，最後計算埃及斑蚊之存活率。實驗結果顯示埃及斑蚊的Spz6 被抑制之後，感染E. coli 後的存活率有明顯的上升，因此我們推測Spz6 參與埃及斑蚊體內的IMD pathway 的調控且可能是扮演負調控子的角色。此外，我們也分析埃及斑蚊Spz6 被抑制的時候，分別感染S.aureus 和E. coli 後，再去偵測IMD pathway 下游基因Cecropin A 和Defencin A的表現，實驗結果顯示，在Spz6 被抑制時，再感染E. coli 的情況下，所偵測到的Cecropin A 和Defencin A 表現量和控制組比較起來都是過量表現。接著我們再進一步去分析Spz6 在埃及斑蚊體內不同組織表現的情形，在埃及斑蚊分別感染S. aureus 和E. coli 後，以Real-Time PCR 去偵測Spz6 在不同組織的表現量，實驗結果顯示當細菌感染蚊子之後，Spz6 在蚊子中腸的表現量有顯著的升高。此外，我們也利用RT-PCR測詴Spz6 在發育時期的表現，發現Spz6 會在black pupa時期有大量表現，於是我們也利用RNAi 的方式，在white pupae 時期去抑制Spz6的表現，結果我們發現蚊子的羽化率和羽化時間分別有明顯的降低及延遲，因此我們推測Spz6 在蚊子的發育可能也扮演重要的角色。根據以上的實驗結果，我們發現Spz6 在埃及斑蚊體內扮演者兩樣功能的角色，一個是免疫調控，另一個則是調控其發育。未來，我們希望釐清Spz6 在IMD signal pathway 與其他因子的相對關係以及Spz6 在轉譯表現的模式，並去瞭解Spz6 在變態(Metamorphosis)方面所扮演的角色。 | zh_TW |
| dc.description.abstract | Mosquitoes are major vectors of several devastating human diseases, such as malaria, Dengue fever, West Nile encephalitis, Japanese encephalitis and filariasis. In addition to developing new drugs and vaccines and controlling these diseases researcher have also been devoted to combat the vector-borne diseases by developing new genetic engineering approaches. Therefore, studies of the regulation machinery of mosquito innate immunity against pathogens and development provide new insights on vector-born disease control. In this study, we use the major vector of Dengue fever, the mosquito Aedes aegypti, as our experimental model. Microarray analysis in Anopheles gambiae showed that several immune responsive genes, including Spätzle-6(Spz6), were up-regulated upon immune stimulation including Inhibitor of Apoptosis-2(IAP2). IAP2 was shown to be an important component participated in the regulation of antimicrobial peptides in the mosquito. In addition, studies in Drosophila melanogaster showed that Spz gene family plays important roles in egulating Toll signaling pathway and the production of antimicrobial peptides. These studies trigger our interest to study the role of Spz6 in the mosquito Aedes aegypti. To understand the role of Aedes aegypti Spz6 in response to invading pathogens, we used RNA interference(RNAi) approach to inhibit the expression of Aedes aegypti Spz6. Survival assay was performed following the challenge of Beauveria bassiana, Staphylococcus aureus or Escherichia coli. The results showed that Aedes aegypti were significantly resistant to E. coli challenge in the absence of Spz6, suggesting that Spz6 acted as an inhibitory regulator in the IMD pathway. Next, we are intrigued to know the effects of Spz6 to the downstream targets of IMD pathway. Therefore, we examined the transcriptional expression profiles of Cecropin A and Defencin A in the absence of Spz6. Interestingly, both Cecropin A and Defencin A showed relatively higher expressional pattern upon S. aureus and E. coli challenge in the absence of Spz6. We further analyzed the Spz6 expressional pattern in different tissues. To our surprise, Spz6 was highly expressed in the mosquito midgut, whereas other Spz members were expressed in the mosquito ovary. On the other hand, we found that Spz6 is highly expressed in the black pupae stage. Therefore, we performed RNAi approach to inhibit the expression of the Spz6 in white pupae stage. We observed the significant reduction of the emerging rate of the adult mosquito in the absence of Spz6. Interestingly, we found that Spz6 process alternative splicing during metamorphosis. The oviposition rate was also reduced in the absence of Spz6. Taken together, our results showed that Spz6 is a negative regulator in the IMD pathway and participate in the vitellogenesis and development. Detail analysis of Spz6 will benefit the development of new strategies for the control of the vector-borne disease. | en |
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| dc.description.tableofcontents | 誌謝................................................................................................................................i
中文摘要........................................................................................................................ii 英文摘要......................................................................................................................iv 第一章 緒論 1.1 蚊子傳播的疾病(mosquito-borne disease)...............................................1 1.1.1 登革熱簡介......................................................................................1 1.1.2 台灣登革熱之流行病學..................................................................1 1.2 雙翅目昆蟲目前對於免疫方面的研究和瞭解之概論...........................3 1.3 目前對埃及斑蚊發育方面的研究...........................................................4 1.3.1 卵黃生成(Vitellogenesis)................................................................4 1.3.2 變態(Metamorphosis)......................................................................6 1.4 Spätzle-6.....................................................................................................8 1.4.1 如何發現Spätzle-6..........................................................................8 1.4.2 Spätzle 簡介......................................................................................8 1.4.3 目前Spätzle在埃及斑蚊的研究......................................................9 1.4.4 目前Spätzle在其他物種的研究......................................................9 1.5 實驗動機與研究目的.............................................................................10 第二章 材料與方法 2.1 埃及斑蚊的培養....................................................................................11 2.2演化樹(Phylogenetic tree)、序列比對(Sequence alignment)和蛋白質結 構預測............................................................................................................11 2.3 製作製備dsRNA 所需的RNAi clone..................................................11 2.4 Double-stranded RNA(dsRNA)製備與純化...........................................13 vii 2.5 Knockdown 之方法.................................................................................14 2.6 感染埃及斑蚊之方法.............................................................................14 2.7 RNA 萃取及RT-PCR..............................................................................14 2.8 即時核酸序列定量（Real-Time PCR）................................................16 2.9 分別建構s7,cecropin,defence full length sequence plasmid.................18 2.10 產卵率實驗（Oviposition assay）...........................................................19 2.11 西方點墨法（Western blotting）.............................................................19 2.12 羽化率實驗(Emerge rate assay)...........................................................20 第三章 結果 3.1 Spz 的演化樹分析(Phylogenetic tree of Spz)..........................................21 3.2 Spz6 的保留區域(Conserved region of Spz6).........................................21 3.3 感染Staphylococcus aureus 和Escherichia coli 之後的存活率分析....21 3.4 感染Staphylococcus aureus 和Escherichia coli 之後Anti-microbial peptide 的mRNA 表現....................................................22 3.5 感染Staphylococcus aureus 和Escherichia coli 之後Spz6 的mRNA 表現..........................................23 3.6 在埃及斑蚊不同時期Spz6 的表現........................................................23 3.7 埃及斑蚊Spz6 蛋白質的表現...............................................................24 3.8 產卵率實驗(Oviposition assay)..............................................................24 3.9 羽化率實驗(Emerge rate assay).............................................................25 3.10 抑制Spz6 對於metamorphosis 相關基因造成的影響......................25 第四章 討論 4.1 蚊子和果蠅的Spz6 在演化上的關係....................................................26 viii 4.2 埃及斑蚊體內的Spz6 在對抗病原菌上所扮演的角色........................26 4.3 埃及斑蚊在不同的發育時期Spz6 的表現(mRNA 和蛋白質方面).....27 4.4 埃及斑蚊的Spz6 在卵黃生成扮演的角色............................................27 4.5 埃及斑蚊的Spz6 在變態扮演的角色....................................................28 4.6 結論.........................................................................................................29 圖表與說明..................................................................................................................30 參考文獻......................................................................................................................45 | |
| dc.language.iso | zh-TW | |
| dc.subject | 變態 | zh_TW |
| dc.subject | 埃及斑蚊 | zh_TW |
| dc.subject | Sp&auml | zh_TW |
| dc.subject | tzle-6 | zh_TW |
| dc.subject | 卵黃生成 | zh_TW |
| dc.subject | Aedes aegypti | en |
| dc.subject | Metamorphosis | en |
| dc.subject | Vitellogenesis | en |
| dc.subject | tzle-6 | en |
| dc.subject | Sp&auml | en |
| dc.title | 埃及斑蚊Spätzle-6 在免疫反應和發育之雙重角色 | zh_TW |
| dc.title | Dual roles of Spätzle-6 in the immune responses and development in the mosquito, Aedes aegypti | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 徐立中,顧家綺,蔡坤憲 | |
| dc.subject.keyword | 埃及斑蚊,Sp&auml,tzle-6,卵黃生成,變態, | zh_TW |
| dc.subject.keyword | Aedes aegypti,Sp&auml,tzle-6,Vitellogenesis,Metamorphosis, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-05 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 未授權公開取用 | 2.02 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。