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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45656完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
| dc.contributor.author | Sheng-Shun Wang | en |
| dc.contributor.author | 王聖舜 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:33:05Z | - |
| dc.date.available | 2019-08-20 | |
| dc.date.copyright | 2009-08-21 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-19 | |
| dc.identifier.citation | 1. Lee WM. Medical progress: Drug-induced hepatotoxicity. New England Journal of Medicine 2003;349:474-485.
2. Lee WM. Acetaminophen and the US Acute Liver Failure Study Group: Lowering the risks of hepatic failure. Hepatology 2004;40:6-9. 3. Palmes D, Spiegel HU. Animal models of liver regeneration. Biomaterials 2004;25:1601-1611. 4. Abella ID. Optical Double-Photon Absorption in Cesium Vapor. Physical Review Letters 1962;9:453-&. 5. Denk W, Strickler JH, Webb WW. 2-Photon Laser Scanning Fluorescence Microscopy. Science 1990;248:73-76. 6. Centonze VE, White JG. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophysical Journal 1998;75:2015-2024. 7. Li FC, Liu Y, Huang GT, Chiou LL, Liang JH, Sun TL, Dong CY, Lee HS. In vivo dynamic metabolic imaging of obstructive cholestasis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology 2009;296:G1091-G1097. 8. Boyd RW. Nonlinear optics. Academic, 2008. 9. Franken PA, Weinreich G, Peters CW, Hill AE. Generation of Optical Harmonics. Physical Review Letters 1961;7:118-&. 10. Young B, Wheater PR. Wheater's functional histology : a text and colour atlas. Churchill Livingstone, 2006. 11. Ross MH, Pawlina W. Histology : a text and atlas : with correlated cell and molecular biology. Lippincott Wiliams & Wilkins, 2006. 12. Fawcett DW, Jensh RP, Bloom W. Bloom & Fawcett's concise histology. Arnold ; Distributed in the USA by Oxford University Press, 2002. 13. Heard KJ. Acetylcysteine for acetaminophen poisoning. New England Journal of Medicine 2008;359:285-292. 14. Kudo A, Kashiwagi S, Kajimura M, Yoshimura Y, Uchida K, Arii S, Suematsu M. Kupffer cells alter organic anion transport through multidrug resistance protein 2 in the post-cold ischemic rat liver. Hepatology 2004;39:1099-1109. 15. Breeuwer P, Drocourt JL, Bunschoten N, Zwietering MH, Rombouts FM, Abee T. Characterization of Uptake and Hydrolysis of Fluorescein Diacetate and Carboxyfluorescein Diacetate by Intracellular Esterases in Saccharomyces-Cerevisiae, Which Result in Accumulation of Fluorescent Product. Applied and Environmental Microbiology 1995;61:1614-1619. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45656 | - |
| dc.description.abstract | 乙醯胺酚為世界上通用的止痛藥與退熱劑。在美國，過量使用普拿疼是藥物造成肝破壞的主因。利用非侵入式多光子顯微術和藉由一級速度模型解析6-CFDA在肝臟細胞的強度變化，讓我們看到肝臟真正的代謝功能。我們觀察過量使用普拿疼24、48、72、96小時後肝臟細代謝的情形。另外，我們利用SYTOX Blue去準確標定死亡的肝細胞，並且比較正常與死亡肝細胞在代謝上的差異。藉由這些技術，我們可以更進一步的研究肝臟的各種疾病與機制。 | zh_TW |
| dc.description.abstract | Acetaminophen (APAP) is a widely used as an analgesic and antipyretic agent. However, the overdose APAP ingestion is also the leading cause of drug-induced liver failure in the United States. Since the metabolic function of liver can be observed by intravital multiphoton microscopy using the intensity variation of 6-carboxyfluorescein diacetate, we used this approach to study the response of liver metabolic function from overdose of APAP after 24, 48, 72, and 96 hr administration. In addition, we used a first-order kinetic model to quantify the extent of liver regeneration. Furthermore, we used SYTOX Blue nucleic acid stain to indicate the dead hepatocytes and found that the uptake and export ability are very different between healthy and dead hepatocytes. Our methodology allows the relationship between liver diseases and metabolic mechanisms to be studied in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:33:05Z (GMT). No. of bitstreams: 1 ntu-98-R96222024-1.pdf: 5015727 bytes, checksum: 16faa0e5e49b3248a7f42a4e4678341d (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 致謝 i
Abstract ii 中文摘要 iii Table of Contents iv Figure Catalog v Chapter 1. Introduction 1 Chapter 2. Basic Principles 5 2.1. Two Photon Excitation 5 2.2. Second Harmonic Generation 10 2.3. Mode locking 13 2.4. Point Spread Function 14 2.5. Resolution 18 Chapter 3. Histology of Liver 21 3.1. Liver Structure and Function 21 3.2. Circulation System in Liver 25 3.3. Cells of Liver 28 Chapter 4. Experimental Setup, Materials and Methods 30 4.1. Acetaminophen Doing 30 4.2. Fluorescent Probes 32 4.3. First-Order Kinetic Model 33 4.4. Liver Imaging Chamber 36 4.5. Experimental Setup 38 4.6. Animal Model and Treatments 40 Chapter 5. Results and Discussion 42 5.1. Visualizing the Effects of APAP on Hepatic Excretory Function 42 5.2. Metabolic Differences between Normal and Dead Hepatocytes 53 Chapter 6. Conclusions 57 References 59 | |
| dc.language.iso | en | |
| dc.subject | 乙醯胺酚 | zh_TW |
| dc.subject | 肝膽功能 | zh_TW |
| dc.subject | 非侵入式多光子顯微術 | zh_TW |
| dc.subject | intravital multiphoton microscopy | en |
| dc.subject | hepatobiiary function | en |
| dc.subject | Acetaminophen | en |
| dc.title | 利用非侵入式多光子顯微術研究乙醯胺酚對肝臟的破壞 | zh_TW |
| dc.title | Utilizing Intravital Multiphoton Microscopy to Investigate Acetaminophen-Induced Liver Injury | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 李宣書(Hsuan-Shu Lee) | |
| dc.contributor.oralexamcommittee | 陳培哲(Pei-Jer Chen),陳永芳 | |
| dc.subject.keyword | 乙醯胺酚,非侵入式多光子顯微術,肝膽功能, | zh_TW |
| dc.subject.keyword | Acetaminophen,intravital multiphoton microscopy,hepatobiiary function, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-19 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 物理研究所 | zh_TW |
| 顯示於系所單位： | 物理學系 | |
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