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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45635完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 董成淵 | |
| dc.contributor.author | Kuang-Yu Liu | en |
| dc.contributor.author | 劉光育 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:31:47Z | - |
| dc.date.available | 2019-08-20 | |
| dc.date.copyright | 2009-08-21 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-19 | |
| dc.identifier.citation | 1 Denk, W., Strickler, J.H., & Webb, W.W., 2-Photon Laser Scanning Fluorescence Microscopy. Science 248 (4951), 73-76 (1990).
2 Li, F.C. et al., In vivo dynamic metabolic imaging of obstructive cholestasis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology 296 (5), G1091-G1097 (2009). 3 Ross, M.H., Kaye, G.I., & Pawlina, W., Histology : a text and atlas, 4th ed. (Lippincott Williams & Wilkins, Philadelphia, Pa., 2003). 4 Lehninger, A.L., Nelson, D.L., & Cox, M.M., Lehninger principles of biochemistry, 4th ed. (W.H. Freeman, New York, 2005). 5 Weiss, T.F., Cellular biophysics. (MIT Press, Cambridge, Mass., 1996). 6 Valeur, B., Molecular fluorescence : principles and applications. (Wiley-VCH, Weinheim, 2002). 7 Boyd, R.W., Nonlinear optics, 3rd ed. (Academic, London, 2008). 8 Niemz, M.H. & SpringerLink (Online service) Laser-Tissue Interactions Fundamentals and Applications in Biological and Medical Physics, Biomedical Engineering, (Springer-Verlag Berlin Heidelberg, Berlin, Heidelberg, 2007). 9 Born, M. & Wolf, E., Principles of optics : electromagnetic theory of propagation, interference and diffraction of light, 7th (expanded) ed. (Cambridge University Press, Cambridge, UK ; New York ;, 1999). 10 Diaspro, A., Confocal and two-photon microscopy : foundations, applications, and advances. (Wiley-Liss, New York, 2002). 11 Cantz, T., Nies, A.T., Brom, M., Hofmann, A.F., & Keppler, D., MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells. American Journal of Physiology-Gastrointestinal and Liver Physiology 278 (4), G522-G531 (2000). 12 Haugland, R.P., Spence, M.T.Z., & Johnson, I.D., Handbook of fluorescent probes and research chemicals, 6th ed. (Molecular Probes, Eugene, OR, USA (4849 Pitchford Ave., Eugene 97402), 1996). 13 Hoefel, D., Grooby, W.L., Monis, P.T., Andrews, S., & Saint, C.P., A comparative study of carboxyfluorescein diacetate and carboxyfluorescein diacetate succinimidyl ester as indicators of bacterial activity. Journal of Microbiological Methods 52 (3), 379-388 (2003). 14 Lue, N. et al., Tissue refractometry using Hilbert phase microscopy. Optics Letters 32 (24), 3522-3524 (2007). | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45635 | - |
| dc.description.abstract | 雙光子螢光顯微術自西元1990年以來被廣泛應用活體影像的研究上。利用其非侵入性和光學切片的特性，搭配活體肝臟觀測平台，可得到高影像品質的肝臟動態資訊。為了探討肝臟的基礎生物物理，嘗試定量活體內肝細胞代謝的分子數量，在得到肝臟代謝趨勢的同時，還能提供肝細胞分子數量的實際改變。
以6-carboxyfluoescein diacetate為試劑，藉由觀測小鼠肝細胞將6-carboxyfluorescein diacetate酯化成6-carboxyfluorescein並排出細胞的過程，將肝細胞內6-carboxyfluorescein的螢光訊號針對肝臟組織與球面相差造成的訊號衰減進行校正後，與已知濃度的6-carboxyfluorescein螢光劑進行訊號強度比較，推得肝臟代謝期間肝細胞內6-carboxyfluorescein的最大分子濃度約在10~100μM，並乘上估計的肝細胞體積5000μm3，得到肝臟代謝期間內一個肝細胞代謝的最大6-carboxyfluorescein分子數量約在107到108個左右。 | zh_TW |
| dc.description.abstract | Two photon fluorescence microscopy has been extensively employed on in vivo imaging since A.D.1990. It is possible to get the high quality dynamic imaging of liver by combining its non-invasive property and optical sectioning ability with liver chamber. In order to investigate fundamental biophysics of liver, try to quantify molecule metabolism of hepatocyte to offer the actual change of the molecule quantity of hepatocyte while getting the trend of hepatic metabolism.
Using 6-carboxyfluorescein diacetate as reagent, observe the process of 6-carboxyfluorescein diacetate esterifies into 6-carboxyfluorescein in mouse hepatocyte. Correcting the signal attenuation of 6-carboxyfluorescein in hepatocyte induced by absorption and scattering of liver tissue and spherical aberration caused of refractive index mismatch, compare it with fluorescent intensity of known concentration of 6-carboxyfluorescein to get the maximum molecule concentration in hepatocyte is 10~100μM during hepatic metabolism. The maximum molecular number of 6-carboxyfluorescein in each hepatocyte is 107 to 108 by multiplying the volume of hepatocyte 5000μm3 to the concentration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:31:47Z (GMT). No. of bitstreams: 1 ntu-98-R96222036-1.pdf: 1664701 bytes, checksum: cee34b569b3cea82d86b833257044404 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 摘要 i
Abstract ii 目錄 iii 圖目錄 v 表目錄 vi 第1章 緒論 1 第2章 肝的生理 3 2-1 肝的構造 3 2-2 肝臟的血液供應與膽道樹 6 2-3 肝的結構組成 8 2-4 細胞膜的運輸 9 第3章 實驗原理 12 3-1 螢光 12 3-2 雙光子激發 14 3-3 二倍頻 19 3-4 點擴函數與數位孔徑 20 第4章 實驗儀器與材料 25 4-1 實驗儀器 25 4-2 實驗動物與肝臟視窗裝設 27 4-3 螢光藥劑 29 4-4 實驗方法概述 30 第5章 實驗結果與分析 32 5-1 數據分析 32 5-2 訊號校正 32 5-3 肝細胞代謝分子數目 35 第6章 結論與未來展望 39 誌謝 40 參考文獻 41 | |
| dc.language.iso | zh-TW | |
| dc.subject | 量化 | zh_TW |
| dc.subject | 雙光子顯微術 | zh_TW |
| dc.subject | 活體 | zh_TW |
| dc.subject | 肝臟代謝 | zh_TW |
| dc.subject | 訊號衰減 | zh_TW |
| dc.subject | quantification | en |
| dc.subject | signal attenuation | en |
| dc.subject | hepatic metabolism | en |
| dc.subject | in vivo | en |
| dc.subject | two photon microscopy | en |
| dc.title | 利用多光子顯微術量化活體內肝細胞分子代謝 | zh_TW |
| dc.title | Utilizing Multiphoton Microscopy to Quantify Molecular Metabolism of Hepatocyte In Vivo | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 李宣書 | |
| dc.contributor.oralexamcommittee | 張顏暉,曹培熙 | |
| dc.subject.keyword | 雙光子顯微術,活體,肝臟代謝,訊號衰減,量化, | zh_TW |
| dc.subject.keyword | two photon microscopy,in vivo,hepatic metabolism,signal attenuation,quantification, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-19 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 物理研究所 | zh_TW |
| 顯示於系所單位： | 物理學系 | |
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