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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45223完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
| dc.contributor.author | Yuan-Syuan Guo | en |
| dc.contributor.author | 郭源軒 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:09:35Z | - |
| dc.date.available | 2014-02-11 | |
| dc.date.copyright | 2010-02-11 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-02-02 | |
| dc.identifier.citation | 李百淵、池雙慶、戴謙、羅玲玲、林恩仲。(2000)。動物育種學解析。國立編譯館。
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Hardcover Wilson, J., Leitao, A., Gonlves, A., Ferreira, C., Reis-Santos, P., Fonseca, A., da Silva, J., Antunes, J., Pereira-Wilson, C. and Coimbra, J. (2007). Modulation of branchial ion transport protein expression by salinity in glass eels (Anguilla anguilla L.). Mar Biol 151, 1633-1645. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45223 | - |
| dc.description.abstract | 莫三比克吳郭魚(Oreochromis mossambicus)存在著耐鹽性不同的品系，造成耐鹽性不同的原因至今仍不清楚，而篩選耐鹽品系吳郭魚對水產養殖上非常重要。經由大量育種選殖出急速轉移到27 ppt 海水中24 小時死亡率低於30 %的耐鹽品系吳郭魚。利用生物晶片比較野生種和耐鹽品系吳郭魚鰓上基因在轉移淡海水的差異，發現大部分被刺激表現的基因的功能為能量代謝和轉錄與轉譯。即時定量聚合酶反應的結果顯示轉移海水後atp1a1 和 slc12a2 在耐鹽品系在3 小時比起野生種有較高的表現量，而slc12a3 在耐鹽品系中明顯下降比野生種迅速。在能量方面基因，耐鹽品系糖解作用相關酵素enolase 1 (eno1)在轉移海水後3 小時有大量表現，而丙酮酸和乳酸在鰓的含量在耐鹽品系皆高於野生種吳郭魚。綜上所述，耐鹽品系較能適應急速鹽度轉移的原因，可能是能更快進行能量代謝及調節運輸蛋白的表現。 | zh_TW |
| dc.description.abstract | Several strains of tilapia (Oreochromis mossambicus) with different salinity tolerances have been found in the field, and the mechanisms of salinity-resistant (SR) are still unknown. Isolation of SR tilapia is very important for aquaculture. In the present study, we identified SR strain tilapia, which exhibits less than 30% mortality after transferred to 27 ppt sea water (SW) for 24 hr. Microarray analysis was used to compare gill gene profiles between the SR strain and wild type (WT) tilapia after SW
transfer. Most of the up-regulated genes are related to energy metabolism, transcription and translation. Quantitative-real time PCR (qPCR) was used to confirm the mRNA expression of some genes selected from the profile list. At 3 hr after SW transfer, the mRNA expression of atp1a1 and slc12a2 was increased in the SR strain that in WT, while that of slc12a3 was reduced in the SR. eno1, a glycolysis gene, was higher in the expression in the RS than in WT. On the other hand, pyruvate and lactate contents in the SR tilapia were higher than those in WT ones. Taken all together, the SR strain of tilapia appear to supply energy and stimulate ion transporters at a better timing and thus survive better than do WT upon SW challenge. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:09:35Z (GMT). No. of bitstreams: 1 ntu-99-R96b41016-1.pdf: 614147 bytes, checksum: 902d373b394cec28f2a929eba40c62a4 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目次
中文摘要----------------------------------------------------- 1 英文摘要----------------------------------------------------- 2 前言--------------------------------------------------------- 3 耐塩吳郭魚對水產養殖的重要性----------------------------- 3 功能性基因體在生物學上的應用----------------------------- 3 魚類為研究環境基因體的好材料----------------------------- 5 硬骨魚類適應海水的滲透壓調節機制------------------------- 6 硬骨魚類面臨滲透壓時的能量代謝--------------------------- 7 研究目的----------------------------------------------------- 9 材料方法----------------------------------------------------- 10 實驗動物------------------------------------------------- 10 選拔耐鹽品系吳郭魚--------------------------------------- 10 吳郭魚之海水轉移----------------------------------------- 10 吳郭魚之全量RNA 抽取------------------------------------- 11 探針製作------------------------------------------------- 11 雜交反應和數據分析--------------------------------------- 12 及時定量聚合酶鏈所反應----------------------------------- 12 冷凍切片------------------------------------------------- 13 原位雜交------------------------------------------------- 13 細胞免疫螢光染色法--------------------------------------- 14 葡萄糖、丙酮酸和乳酸測定比色----------------------------- 14 統計分析方法--------------------------------------------- 14 結果--------------------------------------------------------- 15 耐塩種品系吳郭魚之選拔----------------------------------- 15 微陣列晶片雜合結果--------------------------------------- 15 以即時聚合酶連鎖反應確認微矩陣晶片雜合的準確性----------- 16 與離子運輸蛋白相關基因在吳郭魚轉移海水時的表現----------- 16 與能量代謝相關基因在吳郭魚轉移海水時的表現--------------- 16 在海水轉移後鰓上葡萄糖、丙酮酸、和乳酸的含量變化--------- 17 eno1 基因的定位------------------------------------------ 17 討論--------------------------------------------------------- 18 結論--------------------------------------------------------- 24 參考文獻----------------------------------------------------- 25 附表--------------------------------------------------------- 31 附圖--------------------------------------------------------- 35 附錄(縮寫表)------------------------------------------------- 45 | |
| dc.language.iso | zh-TW | |
| dc.subject | 離子運輸蛋白 | zh_TW |
| dc.subject | 微陣列晶片 | zh_TW |
| dc.subject | 耐鹽品系 | zh_TW |
| dc.subject | 吳郭魚 | zh_TW |
| dc.subject | 能量代謝 | zh_TW |
| dc.subject | energy metabolism | en |
| dc.subject | ion transporter | en |
| dc.subject | microarray | en |
| dc.subject | salinity-resistant | en |
| dc.subject | tilapia | en |
| dc.title | 海水轉移對耐鹽性不同吳郭魚鰓上基因表現之影響 | zh_TW |
| dc.title | Effects of sea water transfer on gill gene expressions in tilapia (Oreochromis mossambicus) with different salinity tolerance | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 黃鵬鵬(Pung-Pung Hwang) | |
| dc.contributor.oralexamcommittee | 陳俊宏(Jiun-Hong Chen),吳淑美(Su-mei Wu) | |
| dc.subject.keyword | 微陣列晶片,耐鹽品系,吳郭魚,能量代謝,離子運輸蛋白, | zh_TW |
| dc.subject.keyword | microarray,salinity-resistant,tilapia,energy metabolism,ion transporter, | en |
| dc.relation.page | 45 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-02-03 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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