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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李君男 | |
| dc.contributor.author | Li-Yi Wong | en |
| dc.contributor.author | 翁立佾 | zh_TW |
| dc.date.accessioned | 2021-06-15T03:54:40Z | - |
| dc.date.available | 2016-10-07 | |
| dc.date.copyright | 2011-10-07 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-22 | |
| dc.identifier.citation | 參考文獻
1. Adler JL & Zickl R. 1969. Winter vomiting disease. J Infect Dis. 119:668-673. 2. Ambert-Balay K, Bon F, Le Guyader F, et al. 2005. Characterization of new recombinant noroviruses. J Clin Microbiol. 43:5179-5186. 3. Anderson AD, Heryford AG, Sarisky JP, et al. 2003. A waterborne outbreak of Norwalk-like virus among snowmobilers-Wyoming, 2001. J Infect Dis. 187:303-306. 4. Ando T, Monroe SS, Gentsch JR, et al. 1995. Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization. J Clin Microbiol. 33:64-71. 5. Ando T, Noel JS, Fankhauser RL. 2000. Genetic classification of ‘‘Norwalk-like viruses’’. J Infect Dis. 181(Suppl 2):S336-S348. 6. Asanaka M, Atmar RL, Ruvolo V, et al. 2005. Replication and packaging of Norwalk virus RNA in cultured mammalian cells. Proc Natl Acad Sci U S A. 102:10327-10332. 7. Atmar RL & Estes MK. 2001. Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses. Clin Microbiol Rev. 14:15-37. 8. Atmar RL & Estes MK. 2006. The Epidemiologic and Clinical Importance of Norovirus Infection. Gastroenterol Clin North Am. 35:275-90, viii. 9. Belliot G, Kamel AH, Estienney M, et al. 2010. Evidence of emergence of new GGII.4 norovirus variants from gastroenteritis outbreak survey in France during the 2007-to-2008 and 2008-to-2009 winter seasons. J Clin Microbiol. 48:994-998. 10. Blanton LH, Adams SM, Beard RS, et al. 2006. Molecular and epidemiologic trends of caliciviruses associated with outbreaks of acute gastroenteritis in the United States, 2000-2004. J Infect Dis. 193:413-421. 11. Bok K, Abente EJ, Realpe-Quintero M, et al. 2009. Evolutionary dynamics of GII.4 noroviruses over a 34-year period. J Virol. 83:11890-11901. 12. Boom R, Sol CJ, Salimans MM, et al. 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 28:495-503. 13. Bruggink LD & Marshall JA. 2009. Molecular and epidemiological features of GIIb norovirus outbreaks in Victoria, Australia, 2002-2005. J Med Virol. 81:1652-1660. 14. Buesa J, Collado B, Lopez-Andujar P, et al. 2002. Molecular epidemiology of caliciviruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain. J Clin Microbiol. 40:2854-2859. 15. Bull RA, Hansman GS, Clancy LE, et al. 2005. Norovirus recombination in ORF1/ORF2 overlap. Emerg Infect Dis. 11:1079-1085. 16. Bull RA, Tanaka MM, White PA. 2007. Norovirus recombination. J Gen Virol. 88:3347-3359. 17. Bull RA, Tu ET, McIver CJ, et al. 2006. Emergence of a new norovirus genotype II.4 variant associated with global outbreaks of gastroenteritis. J Clin Microbiol. 44:327-333. 18. Bull RA, Eden JS, Rawlinson WD, et al. 2010. Rapid evolution of pandemic noroviruses of the GII.4 lineage. PLoS Pathog. 6:e1000831. 19. Burton-MacLeod JA, Kane EM, Beard RS, et al. 2004. Evaluation and comparison of two commercial enzymelinked immunosorbent assay kits for detection of antigenically diverse human noroviruses in stool samples. J Clin Microbiol. 42:2587-2595. 20. Calderon-Margalit R, Sheffer R, Halperin T, et al. 2005. A largescale gastroenteritis outbreak associated with Norovirus in nursing homes. Epidemiol Infect. 133:35-40 21. Chan MC, Sung JJ, Lam RK, et al. 2006. Fecal viral load and norovirus-associated gastroenteritis. Emerg Infect Dis. 12:1278-1280. 22. Cheesbrough JS, Green J, Gallimore CI, Wright PA, et al. 2000. Widespread environmental contamination with Norwalk-like viruses (NLV) detected in a prolonged hotel outbreak of gastroenteritis. Epidemiol Infect. 125:93-98. 23. Chen SM, Ni YH, Chen HL, Chang MH. 2006. Microbial etiology of acute gastroenteritis in hospitalized children in Taiwan. J Formos Med Assoc. 105:964-970. 24. Chen SY, Tsai CN, Lai MW, et al. 2009. Norovirus infection as a cause of diarrhea-associated benign infantile seizures. Clin Infect Dis. 48:849-855. 25. Dai YC, Hu GF, Zhang XF, et al. 2011. Molecular epidemiology of norovirus gastroenteritis in children in Jiangmen, China, 2005-2007. Arch Virol. 26. de Bruin E, Duizer E, Vennema H, Koopmans MP. 2006. Diagnosis of Norovirus outbreaks by commercial ELISA or RT-PCR. J Virol Methods. 137:259-264. 27. Dey SK, Phan TG, Mizuguchi M, et al. 2011. Genetic diversity and emergence of norovirus GII/4-2006b in Japan during 2006-2007. Clin Lab. 57:193-199. 28. Donaldson EF, Lindesmith LC, Lobue AD, Baric RS. 2010. Viral shape-shifting: norovirus evasion of the human immune system. Nat Rev Microbiol. 8:231-241. 29. Duizer E, Schwab KJ, Neill FH, et al. 2004. Laboratory efforts to cultivate noroviruses. J Gen Virol. 85:79-87. 30. Fankhauser RL, Monroe SS, Noel JS, et al. 2002. Epidemiologic and molecular trends of 'Norwalk-like viruses' associated with outbreaks of gastroenteritis in the United States. J Infect Dis. 186:1-7. 31. Fukuda S, Sasaki Y, Takao S, Seno M. 2008. Recombinant norovirus implicated in gastroenteritis outbreaks in Hiroshima Prefecture, Japan. J Med Virol. 80:921-928. 32. Gallimore CI, Lewis D, Taylor C, Cant A, et al. 2004. Chronic excretion of a norovirus in a child with cartilage hair hypoplasia (CHH). J Clin Virol. 30:196-204. 33. Garcia C, DuPont HL, Long KZ, Santos JI, Ko G. 2006. Asymptomatic norovirus infection in Mexican children. J Clin Microbiol. 44:2997-3000. 34. Graham DY, Jiang X, Tanaka T, et al. 1994. Norwalk virus infection of volunteers: new insights based on improved assays. J Infect Dis. 170:34-43. 35. Green KY. 2007. Caliciviridae: The noroviruses. In:Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE, editors. Fields virology, 5th edition. Philadelphia: Lippincott Williams & Wilkins, pp. 949–979. 36. Hardy ME. 2005. Norovirus protein structure and function. FEMS Microbiol Lett. 253:1-8. 37. Iritani N, Kaida A, Kubo H, et al. 2010. Molecular epidemiology of noroviruses detected in seasonal outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan, from 1996-1997 to 2008-2009. J Med Virol. 82:2097-2105. 38. Iritani N, Seto Y, Haruki K, et al. 2000. Major change in the predominant type of 'Norwalk-like viruses' in outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan, between April 1996 and March 1999. J Clin Microbiol. 38:2649-2654. 39. Jiang X, Graham DY, Wang K, et al. 1990. Norwalk virus genome cloning and characterization. Science 250:1580-1583. 40. Jiang X, Huang PW, Zhong WM, et al. 1999. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J Virol Methods. 83:145-154. 41. Jiang X, Wang M, Graham DY, et al. 1992. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 66:6527-6532. 42. Jiang X, Wang M, Wang K, Estes MK. 1993. Sequence and genomic organization of Norwalk virus. Virology. 195:51-61. 43. Jiang X, Wilton N, Zhong WM, et al. 2000. Diagnosis of Human Caliciviruses by Use of Enzyme Immunoassays. J Infect Dis. 181(Suppl 2):S349-S359. 44. Johnson PC, Mathewson JJ, DuPont HL, et al. 1990. Multiple-challenge study of host susceptibility to Norwalk gastroenteritis in US adults. J Infect Dis. 161:18-21. 45. Kageyama T, Shinohara M, Uchida K, et al. 2004. Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan. J Clin Microbiol. 42:2988-2995. 46. Kapikian AZ, Wyatt RG, Dolin R, et al. 1972. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 10:1075-1081. 47. Kaplan JE, Gary GW, Baron RC, et al. 1982b. Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis. Ann Intern Med. 96:756-761. 48. Kaplan JE, Schonberger LB, Varano G, et al. 1982a. An outbreak of acute nonbacterial gastroenteritis in a nursing home. Demonstration of person-to-person transmission by temporal clustering of cases. Am J Epidemiol. 116:940-948. 49. Karst SM, Wobus CE, Lay M, et al. 2003. STAT1-dependent innate immunity to a Norwalk-like virus. Science. 299:1575-1578. 50. Katayama K, Shirato-Horikoshi H, Kojima S, et al. 2002. Phylogenetic analysis of the complete genome of 18 Norwalk-like viruses. Virology 299:225-239. 51. Kim MJ & Kao C. 2001. Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA-RNA recombination. Proc Natl Acad Sci U S A. 98:4972-4977. 52. Kojima S, Kageyama T, Fukushi S, et al. 2002. Genogroup-specific PCR primers for detection of Norwalk-like viruses. J Vir Methods. 100:107-114. 53. Koopmans M. 2008. Progress in understanding norovirus epidemiology. Curr Opin Infect Dis. 21:544-552. 54. Kroneman A, Verhoef L, Harris J, et al. 2008. Analysis of integrated virological and epidemiological reports of norovirus outbreaks collected within the Foodborne Viruses in Europe network from 1 July 2001 to 30 June 2006. J Clin Microbiol. 46:2959-2965. 55. La Rosa G, Pourshaban M, Iaconelli M, Muscillo M. 2008. Detection of genogroup IV noroviruses in environmental and clinical samples and partial sequencing through rapid amplification of cDNA ends. Arch Virol. 153:2077-2083. 56. Le Guyader F, Estes MK, Hardy ME, et al. 1996. Evaluation of a degenerate primer for the PCR detection of human caliciviruses. Arch Virol. 141:2225-2235. 57. Lewis D. 1991. Norwalk agent and other small-round structured viruses in the U.K. J Infect. 23:220-222. 58. Lewis D, Ando T, Humphrey CD, et al. 1995. Use of solid-phase immune electron microscopy for classification of Norwalk-like viruses into six antigenic groups from 10 outbreaks of gastroenteritis in the United States. J Clin Microbiol. 33:501-504. 59. Li HY, Jin M, Zhang Q, et al. 2009. Molecular characterization of GIIb recombinants and a novel genotype of Norovirus detected in China. J Infect. 59:215-218. 60. Lindesmith LC, Donaldson EF, Lobue AD, et al. 2008. Mechanisms of GII.4 norovirus persistence in human populations. PLoS Med. 5:e31. 61. Lopman BA, Adak GK, Reacher MH, Brown DW. 2003. Two epidemiologic patterns of norovirus outbreaks: surveillance in England and Wales, 1992-2000. Emerg Infect Dis. 9:71-77. 62. Lopman BA, Reacher MH, Vipond IB, et al. 2004a. Clinical manifestation of norovirus gastroenteritis in health care settings. Clin Infect Dis. 39:318-324. 63. Lopman B, Vennema H, Kohli E, et al. 2004b. Increase in viral gastroenteritis outbreaks in Europe and epidemic spread of new norovirus variant. Lancet. 363:682-688. 64. Lin CY, Chiu NC, Lee HC, et al. 2010. The emerging importance of norovirus as the etiology of pediatric gastroenteritis in Taipei. J Microbiol Immunol Infect. 43:105-110. 65. Matsui SM & Greenberg HB. 2000. Immunity to calicivirus infection. J Infect Dis. 181(Suppl 2):S331-S335. 66. Mattner F, Sohr D, Heim A, et al. 2006. Risk groups for clinical complications of norovirus infections: an outbreak investigation. Clin Microbiol Infect. 12:69-74. 67. Mead PS, Slutsker L, Dietz V, et al. 1999. Food-related illness and death in the United States. Emerg Infect Dis. 5:607-625. 68. Mohamed N, Belak S, Hedlund KO, Blomberg J. 2006. Experience from the development of a diagnostic single tube real-time PCR for human caliciviruses, Norovirus genogroups I and II. J Virol Meth. 132:69-76. 69. Morens DM, Zweighaft RM, Vernon TM, et al. 1979. A waterborne outbreak of gastroenteritis with secondary person to person spread: association with a viral agent. Lancet. 1:964-966. 70. Noel JS, Ando T, Leite JP, et al. 1997. Correlation of patient immune responses with genetically characterized small round-structured viruses involved in outbreaks of nonbacterial acute gastroenteritis in the United States, 1990 to 1995. J Med Virol. 53:372-383. 71. Noel JS, Fankhauser RL, Ando T, et al. 1999. Identification of a distinct common strain of 'Norwalk-like viruses' having a global distribution. J Infect Dis. 179:1334-1344. 72. Okada M, Ogawa T, Kaiho I, Shinozaki K. 2005. Genetic analysis of noroviruses associated with fatalities in healthcare facilities. J Clin Microbiol. 43:4391-4401. 73. Okada M, Ogawa T, Yoshizumi H, et al. 2007. Genetic variation of the norovirus GII-4 genotype associated with a large number of outbreaks in Chiba prefecture, Japan. Arch Virol. 152:2249-52. 74. Okada S, Sekine S, Ando T, et al. 1990. Antigenic characterization of small, round, structured viruses by immune electron microscopy. J Clin Microbiol. 28:1244-1248. 75. Oliver SL, Dastjerdi AM, Wong S, et al. 2003. Molecular characterization of bovine enteric caliciviruses: a distinct third genogroup of noroviruses (Norwalk-like viruses) unlikely to be of risk to humans. J Virol. 77:2789-2798. 76. Pang XL, Preiksaitis JK, Wong S, et al. 2010. Influence of novel norovirus GII.4 variants on gastroenteritis outbreak dynamics in Alberta and the Northern Territories, Canada between 2000 and 2008. PLoS One. 5:e11599. 77. Parker S, Cubitt D, Jiang JX, Estes M. 1993. Efficacy of a recombinant Norwalk virus protein enzyme immunoassay for the diagnosis of infections with Norwalk virus and other human 'candidate' caliciviruses. J Med Virol. 41:179-184. 78. Parrino TA, Schreiber DS, Trier JS, et al. 1977. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 297:86-89. 79. Patel MM, Widdowson MA, Glass RI, et al. 2008. Systematic literature review of role of noroviruses in sporadic gastroenteritis. Emerg Infect Dis. 14:1224-1231. 80. Pletneva MA, Sosnovtsev SV, Green KY. 2001. The genome of hawaii virus and its relationship with other members of the caliciviridae. Virus Genes. 23:5-16. 81. Puustinen L, Blazevic V, Huhti L, et al. 2011. Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007. Epidemiol Infect. 14:1-8. 82. Rabenau HF, Sturmer M, Buxbaum S, et al. 2003. Laboratory diagnosis of Norovirus: which method is the best? Intervirology. 46:232-238. 83. Reuter G, Vennema H, Koopmans M, Szucs G. 2006. Epidemic spread of recombinant noroviruses with four capsid types in Hungary. J Clin Virol. 35:84-88. 84. Richards AF, Lopman B, Gunn A, et al. 2003. Evaluation of a commercial ELISA for detecting Norwalk-like virus antigen in faeces. J Clin Virol. 26:109-115. 85. Richards GP, Watson MA, Fankhauser RL, Monroe SS. 2004. Genogroup I and II noroviruses detected in stool samples by real-time reverse transcription-PCR using highly degenerate universal primers. Appl Environ Microbiol. 70:7179-7184. 86. Rockx B, deWit M, Vennema H, et al. 2002. Natural history of human Calicivirus infection: a prospective cohort study. Clin Infect Dis. 35:246-253. 87. Rockx BH, Bogers WM, Heeney JL, et al. 2005. Experimental norovirus infections in non-human primates. J Med Virol. 75:313-320. 88. Siebenga JJ, Lemey P, Kosakovsky Pond SL, et al. 2010. Phylodynamic reconstruction reveals norovirus GII.4 epidemic expansions and their molecular determinants. PLoS Pathog. 6:e1000884. 89. Siebenga JJ, Vennema H, Renckens B, et al. 2007. Epochal evolution of GGII.4 norovirus capsid proteins from 1995 to 2006. J Virol. 81:9932-9941. 90. Siebenga JJ, Vennema H, Zheng DP, et al. 2009. Norovirus illness is a global problem: emergence and spread of norovirus GII.4 variants, 2001-2007. J Infect Dis. 200:802-812. 91. Shieh Y, Monroe SS, Fankhauser RL, et al. 2000. Detection of norwalk-like virus in shellfish implicated in illness. J Infect Dis. 181(Suppl 2):S360-S366. 92. Tan M & Jiang X. 2005. Norovirus and its histo-blood group antigen receptors: an answer to a historical puzzle. Trends Microbiol. 13:285-293. 93. Teunis PF, Moe CL, Liu P, et al. 2008. Norwalk virus: how infectious is it? J Med Virol. 80:1468-1476. 94. Thornhill TS, Wyatt RG, Kalica AR, et al. 1977. Detection by immune electron microscopy of 26 to 27 nm virus-like particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 135:185-192. 95. Tu ET, Bull RA, Kim MJ, et al. 2008a. Norovirus excretion in an aged-care setting. J Clin Microbiol. 46:2119-2121. 96. Tu ET, Bull RA, Greening GE, et al. 2008b. Epidemics of gastroenteritis during 2006 were associated with the spread of norovirus GII.4 variants 2006a and 2006b. Clin Infect Dis. 46:413-420. 97. van Maarseveen NM, Wessels E, de Brouwer CS, et al. 2010. Diagnosis of viral gastroenteritis by simultaneous detection of Adenovirus group F, Astrovirus, Rotavirus group A, Norovirus genogroups I and II, and Sapovirus in two internally controlled multiplex real-time PCR assays. J Clin Virol. 49:205-210. 98. Vinje J, Hamidjaja RA, Sobsey MD. 2004. Development and application of a capsid VP1 (region D) based reverse transcription PCR assay for genotyping of genogroup I and II noroviruses. J Virol Methods. 116:109-117. 99. Vinje J & Koopmans MP. 1996. Molecular detection and epidemiology of small round-structured viruses in outbreaks of gastroenteritis in The Netherlands. J Infect Dis. 174:610-615. 100. Wolf S, Williamson W, Hewitt J, et al. 2009. Molecular detection of norovirus in sheep and pigs in New Zealand farms. Vet Microbiol. 133:184-189. 101. Worobey M & Holmes EC. 1999. Evolutionary aspects of recombination in RNA viruses. J Gen Virol. 80:2535-2543. 102. Wu FT, Oka T, Katayama K, et al. 2006. Genetic diversity of noroviruses in Taiwan between November 2004 and March 2005. Arch Virol. 151:1319-1327. 103. Wu HM, Fornek M, Schwab KJ, et al. 2005. A norovirus outbreak at a long-term-care facility: the role of environmental surface contamination. Infect Control Hosp Epidemiol. 26:802-810. 104. Wu TC, Liu HH, Chen YJ, et al. 2008. Comparison of clinical features of childhood norovirus and rotavirus gastroenteritis in Taiwan. J Chin Med Assoc. 71:566-570. 105. Yan H, Yagyu F, Okitsu S, et al. 2003. Detection of norovirus (GI, GII), Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR. J Virol Methods. 114:37-44. 106. Yang LC, Chiang PC, Huang TH, et al. 2010. Residents had an increasing risk of norovirus gastroenteritis infection than health care workers during an outbreak in a nursing home. J Am Med Dir Assoc. 11:592-597. 107. Yang SY, Hwang KP, Wu FT, et al. 2010. Epidemiology and clinical peculiarities of norovirus and rotavirus infection in hospitalized young children with acute diarrhea in Taiwan, 2009. J Microbiol Immunol Infect. 43:506-514. 108. Yoon JS, Lee SG, Hong SK, et al. 2008. Molecular epidemiology of norovirus infections in children with acute gastroenteritis in South Korea in November 2005 through November 2006. J Clin Microbiol. 46:1474-1477. 109. Zheng DP, Ando T, Fankhauser RL, et al. 2006. Norovirus classification and proposed strain nomenclature. Virology. 346:312-323. 110. Zheng DP, Widdowson MA, Glass RI, Vinje J. 2010. Molecular epidemiology of genogroup II-genotype 4 noroviruses in the United States between 1994 and 2006. J Clin Microbiol. 48:168-177. 111. 莎日娜 (2007) 台灣諾若病毒重組株之全長基因體序列分析。國立臺灣大學醫學檢驗暨生物技術學研究所碩士論文 112. 吳芳姿 (2006) 病毒性腹瀉症候群分子檢測監測系統之發展及流行病學研究-III。行政院衛生署疾病管制局九十五年度科技研究計畫 113. 吳和生 (2008) 建置腹瀉感染症即時監測系統。行政院衛生署疾病管制局九十七年度科技研究計畫 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44775 | - |
| dc.description.abstract | 諾羅病毒為杯狀病毒科之成員，其基因體為單股正向RNA，由三個開放讀架 (ORF) 組成，在ORF1與ORF2之間有重疊區。ORF1表現包含RNA聚合酶在內等非結構性蛋白質產物，ORF2及ORF3分別轉譯出主要 (VP1) 及次要 (VP2) 結構性外殼蛋白質。諾羅病毒依據聚合酶及外殼蛋白質基因的核酸序列相似性區分為五種基因群 (GI - GV)。與人類疾病有關之GI和GII基因群可分別再細分為8種及17種基因型。諾羅病毒是造成人類病毒性急性腸胃炎的主要致病原之一，所有年齡層皆有可能遭受感染，然而對於嬰幼兒、老年人和免疫功能不全者易造成危害，目前並無有效的抗病毒藥物，感染後也無法獲得終生免疫。根據統計，在開發中國家每年約有二十萬五歲以下孩童死於諾羅病毒的感染。近年來研究顯示諾羅病毒基因組有高度突變和重組的傾向，常導致新興病毒株的產生和大流行。
本研究收集來自台大醫院病毒室1981~1993年及2006~2007年共計3391件糞便檢體，針對外殼蛋白質基因N/S區域之核酸序列 (以下簡稱C區) 進行反轉錄-聚合酶連鎖反應，以檢測是否存在諾羅病毒。PCR陽性檢體再針對部分聚合酶基因序列 (以下簡稱A區) 及C區序列進行核酸定序及基因分型，最後偵測到172個諾羅病毒株，並納入本實驗室之前分析的2002~2005年及2008年初已定序之78個病毒株，進行整體分析，以瞭解北台灣地區諾羅病毒的分子流行病學。 扣除來自相同病人的檢體，239個病毒株中有235個屬於GII基因群 (98.3%)，僅有4個屬於GI基因群 (1.7%)。依據A區及C區序列進行基因分型，以GII.4基因型所佔比例最高 (72.4%)，共計173株，其次為GII.6，共計22株 (9.2%)。不同年代流行之GII.4病毒株有所差異，1988~1992年屬於Camberwell (或Bristol) 變異株；2002年以 Farmington Hills變異株為主，並於上半年偵測到零星幾個US95/96變異株；2004~2005年間之冬季是Hunter變異株；2006~2008年以2006b變異株為主，2006年尚偵測到 Hunter變異株及零星幾個2006a變異株。另於1980年代早期檢出編號395/84/TW之GII.4病毒株，它不屬於前述任一群，其C區序列經BLAST顯示為CHDC2094/74/US 相似株 (核酸相似度94.3%)，但A區序列則接近Bristol/93/UK病毒株 (核酸相似度92.3%)。 A區及C區序列分屬不同型別之疑似重組株共計15個，以GII.b/GII.3和GII.12/GII.4兩種重組型較多。分析10個重組病毒株的ORF1至ORF2核酸序列 (7株病毒已於民國96年莎日娜碩士論文中探討)，顯示重組位點在ORF1/2重疊區附近。 本研究顯示北台灣地區在2002至2008年間，諾羅病毒株的基因序列確實有因年度而發生變化，並與國際流行趨勢相似。而本地早期病毒株395/84/TW是屬於70年代 [CHDC2094/74/US] 或80年代 [Camberwell (或Bristol) 群] 之病毒，或是另一種變異株，仍有待後續基因體全長序列分析，以瞭解病毒之演化。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T03:54:40Z (GMT). No. of bitstreams: 1 ntu-100-R97424025-1.pdf: 11906323 bytes, checksum: cb10b74bbd9d14da7c1fb7dd2ecf3f2b (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員會審定書....................................................................................i
中文摘要...................................................................................................ii 英文摘要...................................................................................................iv 第一章 緒論 …………………………………………………………..1 1.1急性腸胃炎 ...........................................................................1 1.2 諾羅病毒簡介 ......................................................................1 1.3 傳染途徑與臨床症狀 ..........................................................2 1.4 實驗室診斷 ..........................................................................4 1.5 諾羅病毒的分類 ..................................................................5 1.6 流行病學 ..............................................................................6 1.7 研究動機與目的 ..................................................................9 第二章 材料與方法 ............................................................................10 2.1 材料 ....................................................................................10 2.2 檢體前處理 ........................................................................10 2.3 病毒RNA萃取 ...................................................................10 2.4 反轉錄聚合酶連鎖反應 ....................................................12 2.5 洋菜膠體電泳分析 ............................................................13 2.6 PCR產物純化 ....................................................................14 2.7 核酸定序 ............................................................................15 2.8 病毒重組株之分析.....................................................17 第三章 結果 ………………................................................................22 3.1核酸萃取法之建立 ..............................................................22 3.2引子對之選用 ......................................................................23 3.3諾羅病毒在台灣之流行概況 ..............................................24 3.4諾羅病毒之基因分析………………………………………26 3.5重組株之分析 ......................................................................27 第四章 討論 ........................................................................................29 參考文獻 ................................................................................................34 圖 ............................................................................................................43 表 ............................................................................................................56 附錄 ........................................................................................................68 | |
| dc.language.iso | zh-TW | |
| dc.title | 北台灣地區諾羅病毒之分子流行病學研究 | zh_TW |
| dc.title | Molecular Epidemiology of Noroviruses in Northern Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 高全良,張淑媛 | |
| dc.subject.keyword | 諾羅病毒,RNA聚合酶,外殼蛋白質基因N/S區域,GII.4型變異株,病毒重組株, | zh_TW |
| dc.subject.keyword | norovirus,RdRp,capsid N/S region,GII.4 variant,recombinant strain, | en |
| dc.relation.page | 70 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-23 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
| 顯示於系所單位： | 醫學檢驗暨生物技術學系 | |
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|---|---|---|---|
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