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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉懷勝(Hwai-Shen Liu) | |
| dc.contributor.author | Hsiang-Yu Huang | en |
| dc.contributor.author | 黃翔瑜 | zh_TW |
| dc.date.accessioned | 2021-06-15T03:51:31Z | - |
| dc.date.available | 2012-07-21 | |
| dc.date.copyright | 2010-07-21 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-13 | |
| dc.identifier.citation | Bae S., Shoda M. (2004) Bacterial Cellulose Production by Fed-Batch Fermentation in Molasses Medium. Biotechnology Progress 20:1366-1371.
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Journal of Materials Science 24:3141-3145. Yang Y.K., Park S.H., Hwang J.W., Pyun Y.R., Kim Y.S. (1998) Cellulose production by Acetobacter xylinum BRC5 under agitated condition. Journal of Fermentation and Bioengineering 85:312-317. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44593 | - |
| dc.description.abstract | 許多文獻探討如何利用氣舉裝置或攪拌槽的反應器生產細菌纖維素。這個研究是要探討如何以連續式的操作模式來生產纖維素薄膜。
本實驗透過饋料式和連續式的操作來觀察反應器中各項數據變化並同時以批次操作的數據來互相比較。在本實驗中發現，利用兩纖維素薄膜互相連接的特性來減少反應器空間利用的問題。 本實驗於批次操作第四天時開始連續式操作，每三天添加改良後(pH8.0葡萄糖濃度50g/L) 40ml的BSH培養基，並將生產的纖維素薄膜以相隔反應器內培養基0~1mm左右的距離置放，添加八次相同條件培養基後(二十四天)可以得到長度將近170公分且乾重將近2.3g的細菌纖維素。在這其中，每三天大約都保持類似程度的纖維素合成量和長度。 本反應器可以生產任意長度的細菌纖維素薄膜。並提供另一種生產纖維素的模式，同時也看到降低生產纖維素成本的可能性。 | zh_TW |
| dc.description.abstract | Many studies have been conducted to produce bacterial cellulose in the agitated reactors. This research aimed to continuously produce bacterial cellulose pellicles. The experiment was conducted in fed-batch and continuous operation and the results were compared to that in batch operation. The connection of two BC pellicles was found to design the continuous operation of the reactor.
The continuous operation started at the chosen time of the batch operation adding 40ml modified BSH (0.5% yeast extract, 0.5% peptone, 0.115% citric acid, 0.27% Na2HPO4•12H2O and 5% D-glucose pH 8.0) medium every three days produced 170cm, 2.3g bacterial cellulose pellicle(dry basis) after twenty-four days. Before the formed BC pellicle connected to the pellicle synthesized in the following three days, the maximum distance between the formed pellicle and the medium was set at 1mm. The reactor can not only provide a alternative way to produce bacterial cellulose, but also produce arbitrary length of bacterial cellulose pellicle. Also, the reactor may lower the price of bacterial cellulose which can make the price of bacterial cellulose more competitive. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T03:51:31Z (GMT). No. of bitstreams: 1 ntu-99-R97524039-1.pdf: 8564012 bytes, checksum: fa5f64cfcfa03409331437b8148a5a1c (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員審定書 I
致謝 II 摘要 III Abstract IV 目錄 V 表目錄 VII 圖目錄 VIII 第一章 緒論 1 第二章 文獻回顧 2 2-1 細菌纖維素的介紹 2 2-1-1 細菌纖維素結構和化學式 2 2-1-2 細菌纖維素的理化性質 5 2-1-3 細菌纖維素的特性 8 2-1-4 細菌纖纖維素的應用 11 2-2細菌纖維素生產菌株 15 2-2-1生產菌株生理特性 16 2-2-2細菌纖維素的合成路徑 19 2-2-3葡萄糖聚合為β-1,4-glucan鏈 22 2-2-4 β-1,4-glucan鏈聚集形成束狀纖維素 25 2-2-5纖維素合成酶調控機制 28 2-3影響細菌纖維素生成的因素 30 2-3-1培養條件的影響 30 2-3-2 培養方式 35 第三章 實驗流程、方法、藥品及儀器 42 3-1 實驗菌株 42 3-2培養基組成 43 3-3 實驗方法 46 3-3-1 預培養 46 3-3-2 主培養 47 3-3-3 纖維素處理 47 3-4 DNS 試劑檢測還原糖 49 3-5 實驗藥品 52 3-6實驗儀器 54 第四章 實驗結果與討論 55 4-1 環境因子對Gluconacetobacter xylinus生成細菌纖維素產量的影響 …………………………………………………………………55 4-1-1 培養容器表面積的影響 55 4-1-2.1 培養基體積的影響 59 4-1-2.2 以不同體積的BSH培養基進行靜置培養 64 4-1-3 培養溫度的影響 69 4-2 靜置培養中合成纖維素的細菌及纖維素薄膜的連接 75 4-2-1 存在於細菌纖維素的細菌 75 4-2-2利用培養基中細菌作為植菌來源進行批次培養 77 4-2-3纖維素薄膜的連接 81 4-3 一次饋料式操作對於Gluconacetobacter xylinus生成細菌纖維素產量的影響 86 4-3-1剩餘培養基以及預活化作為植菌來源的異同 86 4-3-2 浸泡時間的影響 89 4-3-3選擇批次培養時間進行饋料式培養 93 4-3-4 添加不同培養基葡萄糖濃度的影響 98 4-3-5 添加不同培養基酸鹼度pH的影響 103 4-4連續式操作對於Gluconacetobacter xylinus生成細菌纖維素產量的影響 109 4-4-1連續式操作下添加培養基時間間隔的影響 110 4-4-2連續式操作下添加不同培養基葡萄糖濃度的影響 122 4-4-3連續式操作下添加不同培養基酸鹼度pH的影響 136 第五章 結論 143 參考文獻 146 附錄 150 | |
| dc.language.iso | zh-TW | |
| dc.subject | 細菌纖維素 | zh_TW |
| dc.subject | 連續式生產 | zh_TW |
| dc.subject | 細菌纖維素薄膜連接 | zh_TW |
| dc.subject | bacterial cellulose | en |
| dc.subject | continuous production | en |
| dc.subject | bacterial cellulose pellicles connection | en |
| dc.title | 以Gluconacetobacter xylinus連續式生產細菌纖維素薄膜之研究 | zh_TW |
| dc.title | Continuous Production of Bacterial Cellulose Pellicle by Gluconacetobacter xylinus | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 賴進此,許駿發,王孟菊 | |
| dc.subject.keyword | 細菌纖維素,連續式生產,細菌纖維素薄膜連接, | zh_TW |
| dc.subject.keyword | bacterial cellulose,continuous production,bacterial cellulose pellicles connection, | en |
| dc.relation.page | 154 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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