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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳俊顯 | |
dc.contributor.author | Yao-Min Liu | en |
dc.contributor.author | 劉耀閔 | zh_TW |
dc.date.accessioned | 2021-06-15T03:51:20Z | - |
dc.date.available | 2010-07-26 | |
dc.date.copyright | 2010-07-26 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-13 | |
dc.identifier.citation | (1) Daniel, M.-C.; Astruc, D. Chem. Rev. 2004, 104, 293-346.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44579 | - |
dc.description.abstract | 本論文以目視觀察相變化(phase segregation)偵測特定濃度範圍銅離子。利用半胱胺酸(cysteine, Cys)與銅離子反應產生棉絮狀聚合物(polymer),使溶液變為不均相。實驗發現當[Cu2+]0/[Cys]0介於0.4~0.5時,不均相於3分鐘之內產生,當[Cu2+]0/[Cys]0小於0.4時,不均相產生所需時間隨[Cu2+]0減少而增加,當[Cu2+]0/[Cys]0大於0.5時,不產生不均相。加入奈米金可吸附於棉絮狀聚合物,藉以突顯相變化;或應用廷得耳效應(Tyndall effect),使相變化容易以肉眼觀察。觀察3分鐘內不均相出現與否,判斷銅離子濃度範圍。
藉由半胱胺酸的硫醇官能基(-SH)與二價銅離子(Cu2+)發生氧化還原反應,半胱胺酸被氧化為胱胺酸(cystine),二價銅離子被還原成ㄧ價銅離子(Cu+),Cu+與溶液中未被氧化的半胱胺酸形成1:1錯合物(Cu+Cys),此錯合物將進一步形成如棉絮狀的聚合物[Cu+Cys]n,而使原來均勻溶液相變為不均相。當[Cu2+]0/[Cys]0大於0.5時,將還原出無法與半胱胺酸形成Cu+Cys之過量free Cu+,此free Cu+於溶液中進行自身氧化還原後生成Cu2+及Cu0,Cu2+氧化Cu+Cys錯合物中之Cys影響聚合物之生成,而使溶液維持均相。 | zh_TW |
dc.description.abstract | Visual and rapid determination of whether an analyte falls within or outside of a concentration range has been validated. The target analyte demonstrated in this study is Cu2+ which, among other transition metal ions, receives considerable attention of its biological and environmental importance. Concerning the reactions of cysteine (Cys) and Cu2+ under proper conditions, the colloidal gold solution with Cys and Cu2+ becomes fluffily flocculated in 3 minutes. The fluffy floccules appear reddish and the solution between the floccules becomes colorless owing to the adsorption of gold nanoparticles on the floccules. According to our experimental results and literature studies, the mercapto group from Cys is oxidized due to reducing Cu2+ into Cu+ which be bound with excess Cys and generates Cu+Cys. These Cu+Cys complexes will further form μ-thiolato complex polymers [Cu+Cys]n ascribed to the fluffy floccules and phase segregation. The visualization of the polymerization takes less than 3 minutes for [Cu2+]0/[Cys]0 ranging from 0.4 to 0.5. When [Cu2+]0/[Cys]0 > 0.5, the solution exhibits free Cu+ because of the limited amount of Cys. The Cu2+ is regenerated by the disproportionation of free Cu+. The Cu2+ oxidizes Cys from Cu+Cys monomer. As a result, no phase segregation is observed when [Cu2+]0/[Cys]0 > 0.5. As [Cu2+]0/[Cys]0 < 0.4, the time required for fluffy floccules formation is over 3 minutes. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T03:51:20Z (GMT). No. of bitstreams: 1 ntu-99-R97223130-1.pdf: 1012048 bytes, checksum: fb65bdcb2d91eaee01e92ed4d22f9c4a (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 I
英文摘要 II 總目錄 III 圖目錄 VI 第一章 緒論 1 1-1 前言 1 1-2 奈米金之相關介紹 2 1-2-1 奈米金之簡介 2 1-2-2 奈米金之表面電漿共振(surface plasmon resonance) 3 1-2-3 奈米金之製備方式 7 1-3 銅離子之相關介紹 9 1-3-1 銅離子對人體重要性之簡介 9 1-3-2 銅離子偵測方法之文獻回顧 12 1-3-3 半胱胺酸與銅離子反應之文獻回顧 15 1-4 本論文研究目的 21 第二章 實驗部分 22 2-1 實驗藥品及耗材 22 2-1-1 實驗藥品 22 2-1-2 實驗器材 23 2-2 實驗儀器設備 25 2-3 檸檬酸鹽還原及保護的奈米金之製備 26 2-4 儀器量測 28 2-4-1 UV/Vis的量測 28 2-4-2 粒徑量測 28 2-4-3 TEM的量測 29 2-4-4 XPS的量測 29 2-4-5 ICP-AES的量測 30 2-4-6 電灑法質譜儀(ESI-MS)的量測 31 2-5 溶液的配製 32 2-6 以相變化偵測銅離子 33 2-7 真實樣品的處理 34 第三章 結果與討論 35 3-1 利用半胱胺酸與銅離子反應產生不均相辨識銅離子 35 3-2 棉絮狀懸浮物之性質探討 39 3-3 半胱胺酸與銅離子反應產生相變化之推測機制 41 3-3-1 反應式(1) Cu2+還原為Cu+之驗證 43 3-3-2 反應式(2) Cu+Cys錯合物生成之驗證 45 3-3-3 反應式(3) polymer組成之討論 46 3-3-4 反應式(4) Cu+自身氧化還原之驗證 50 3-3-5 反應式(5)自身氧化還原生成Cu2+與Cu+Cys反應之驗證 52 3-4 應用廷得耳效應以改善偵測極限 54 3-5 真實樣品之量測 56 第四章 結論 58 第五章 參考文獻 59 | |
dc.language.iso | zh-TW | |
dc.title | 利用半胱胺酸與銅離子反應產生不均相辨識特定濃度範圍銅離子 | zh_TW |
dc.title | Visual Detection for a Concentration Range of Cu2+ by Phase Segregation of Cu-Cysteine Complex | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉春櫻,張哲政 | |
dc.subject.keyword | 半胱胺酸,銅離子,不均相, | zh_TW |
dc.subject.keyword | cysteine,Cu,phase segregation, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-13 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
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