口服裂褶多醣對免疫調節反應之影響

Abstract

本實驗主要利用由裂褶菌中萃取出的裂褶多醣，以不同餵食頻率給予正常小鼠及擔癌小鼠，進而探討裂褶多醣對於小鼠的免疫調節及腫瘤抑制的效用。 首先取不同濃度裂褶多醣，0.1 ml餵食正常小鼠一次，隔天犧牲解剖並取出脾臟，實驗結果觀察到其中以餵食0.3 %裂褶多醣溶液，能夠有效提昇小鼠脾臟單核球細胞毒殺能力以及增生效率，因此在接下來的實驗當中，皆選用0.3 %裂褶多醣溶液餵食小鼠，並且搭配不同餵食頻率給予正常小鼠裂褶多醣一周，分別為每日給予、間隔一日給予、間隔兩日給予，並追蹤一周內免疫能力的變化。其中以每日餵食處理，於一周內能夠有效激活小鼠脾臟單核球細胞毒殺能力及增生活性效率較佳，兩天一次的處理，於一周內能夠有效提昇小鼠血清中細胞激素含量效率較好，顯示每日餵食及間隔一日餵食對於正常小鼠免疫能力提升效果較佳。 在小鼠的腫瘤抑制試驗中，分別使用每日餵食及間隔一日餵食的餵食頻率，於小鼠擔癌前七天開始餵食，連續餵食21天後犧牲小鼠並觀察其腫瘤大小與免疫調節能力。其中每日餵食組別抑制腫瘤增殖率較佳，並且檢測擔癌小鼠之脾臟單核球細胞毒殺能力、單核球細胞增生活性以及血清中細胞激素含量等免疫指標，發現有餵食多醣體皆能有效激活擔癌小鼠之各項免疫活性。 綜合以上結果我們可以發現，裂褶多醣能夠提升正常小鼠與擔癌小鼠的免疫活性及抗腫瘤能力，並且多醣的給予頻率確實會影響多醣體的免疫調節活性，其中以使用0.3 %的多醣濃度，搭配每日餵食的頻率，有較佳的免疫調節能力及腫瘤抑制率，因此認為每日給予小鼠0.3 %裂褶多醣為較適當的餵食頻率。

The present study aims to investigate Schizophyllan(SPG), a β-glucan polymer from Schizophyllum commune, efficacy on the activation of immune cells as well as antitumor activity on mice at various delivery frequency levels by oral route. The results on the obserivation of efficacy of immune-enhancement in mice v.s Schizophyllan concentrations show that 0.3 % SPG solution in the optimal concentration for the experiment. It is therefore this concentration is used for the further study to investigate the most effective frequency for three groups of mice administrated with 0.3 % SPG at daily, two or three days interval in one week. The cytotoxicity, cell proliferation of splenic mononucleus cell and concentration of IL-12, IFN-γ in serum for the mice was observed. The results shows that daily administration of 0.3 % SPG for one week is superior then those received with the same dose by 2 days/once or 3 days/once on the enhancement of cytotoxicity activity and increases of splenic mononucleus cell proliferation of experiment mice. However, the highest IL-12 and IFN-γ in serum was observed in those mice received with Schizophyllan at 2 days/once group. In the obserivation of tumor supression, oral administration of Schizophyllan daily or 2 days/once at an experimental intervals, shows daily gave a better results. It is also shows that Schizophyllan signification enhanced cytotoxicity, cell proliferation and cytokine level than control. Considerind all these results, it suggested that oral Schizophyllan at 0.3 %, daily promoted better immunomodulation and antitumor activity.